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Citations for Calbiochem :
451 - 499 of 499 citations for Cell Division Cycle 2 Like Protein Kinase 6 CDC2L6 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... CTR and KO MEF cells were seeded in a 96-well plate (6,000 cells/well) and treated or not with erastin (0.7μM; Calbiochem).
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bioRxiv - Biophysics 2020Quote: ... The cells were washed three times in the washing buffer 1X of OxyDNA Assay Kit (500095, Calbiochem) and incubated overnight at 37°C in FITC-conjugated anti 8-oxoguanine (500095 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated for 40 hours with the pan-FGFR inhibitor PD173074 [100nM] (Calbiochem, Merck Eurolab, France). Control cells were treated with DMSO vehicle diluted proportionally to the inhibitor ...
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bioRxiv - Biochemistry 2022Quote: Neuro2a cells were grown on glass coverslips and incubated with 20 μM nocodazole (Calbiochem, San Diego CA). Coverslips were removed at zero time and 2 hours and were fixed for 10 min using 3% formaldehyde in PBS prior to indirect immunofluorescence analysis as described below ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were again washed with PBS pH 7.4 (3 × 10 min) and mounted in antifade (Mowiol, Calbiochem).
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bioRxiv - Cell Biology 2023Quote: ... GFP-Rab11 WTlow and DNlow cell culture media was also supplemented with 1.25 μg.mL-1 puromycin (Calbiochem). Cells were regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... cells were pretreated 1 h at 37°C with 20 nM bafilomycin A1 (Calbiochem, stock in DMSO) to block endocytosis ...
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bioRxiv - Biophysics 2023Quote: Pharmacologically treated cells were respectively incubated in OM containing 500 nM latrunculin B (Calbiochem, Millipore, Billerica, MA), 20 U/mL cholesterol oxidase (Calbiochem ...
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bioRxiv - Molecular Biology 2021Quote: ... The macrophage cells were induced with 10 ng/ml Escherichia coli O111:B4 LPS (Calbiochem, La Jolla, CA) for activation unless specified otherwise ...
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bioRxiv - Microbiology 2020Quote: ... and overlaid with standard cell culture media plus 0.37% (w/vol) sodium bicarbonate and 0.8% (w/vol) Aquacide II (Millipore Calbiochem). After 7 days incubation at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were infected for 1 h and an overlay of 1% carboxymethylcellulose (Aquacide II, Calbiochem, San Diego, CA) prepared in RPMI was added ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... Following five days the cells were cultured in basal media and 0.1 µM retinoic acid (Fisher)/0.5 µM SAG (Calbiochem) and for the next 7 days ...
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bioRxiv - Neuroscience 2021Quote: Cytoplasmic and nuclear fractions were prepared from cell lysates using a ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: The cells were lysed in radioimmunoprecipitation assay buffer (RIPA buffer) supplemented with phosphatase inhibitor cocktail set-II (Calbiochem) and protease inhibitor (VWR) ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were washed with ice-cold PBS(-/-) with 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131). The PBS was aspirated ...
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bioRxiv - Neuroscience 2021Quote: ... or P5–P6 DRG rat neurons were diluted to 35,000 cells/ml in Sato’s media and treated with either 1 mM dbcAMP (Calbiochem), mixed population or recombinant Histones (1-20µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... standard imaging analysis was performed for cells grown constitutively in the presence of β-estradiol (3nM; Calbiochem 3301).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... collected pRPE cells were suspended in a hypotonic buffer containing protease inhibitor cocktail (MilliporeSigma™ Calbiochem™, 53913110VL). The pRPE cells underwent disruption with a Dounce tissue homogenizer ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with 10 nm of estradiol-17β or 10 μM γ-secretase inhibitor IX (DAPT; Calbiochem/EMD Millipore #565770-5MG ...
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bioRxiv - Cell Biology 2019Quote: ... cells were lysed at 1 × 109 cells/ml in ice-cold lysis buffer (0.1 µM TLCK, 1 µg/ml Leupeptin, Phosphatase Inhibitor Cocktail II (Calbiochem),1 mM PMSF ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells infected with pBABE-PURO B-RAF-ER were cultivated in the presence of OHT (600nM) and PD98059 (Calbiochem) 50μM ...
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bioRxiv - Biochemistry 2021Quote: ... cells were trypsinized and seeded for selection in complete media supplemented with 4 μg/mL puromycin (Calbiochem, Merck Millipore). All cell lines were passaged 3 times before use and kept in culture supplemented with 2 μg/mL puromycin and/or stored in liquid nitrogen.
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bioRxiv - Cell Biology 2022Quote: ... and at 48 hpi cells transduced with Wasabi-pIPFCW2 were selected with 3-4 mg/ml puromycin (Calbiochem, 540411) and sorted for mid-range expression of Wasabi.
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bioRxiv - Immunology 2022Quote: Cell samples or EV samples were lysed in radioimmunoprecipitation assay (RIPA) buffer supplemented with protease inhibitors (Calbiochem, CA, USA) and phosphatase inhibitors (Calbiochem) ...
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bioRxiv - Biophysics 2022Quote: ... Cell pellets were lysed with an Emulsiflex (Avastin) in the presence of protease inhibitor cocktail Mixture Set V (Calbiochem) and DNAse (Millipore Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Selection of clones with successfully integrated knockdown constructs was accomplished by treating infected hPDL cells with puromycin (Calbiochem, USA) at a final concentration of 5 µg/mL for 24-hours in supplemented MEM-α media ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed in mild non-ionic detergents (0.1% Triton X-100 and Deoxycholate) and protease inhibitor cocktail (Calbiochem) to preserve the integrity of histones harbouring epitopes of interest during cell lysis ...
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bioRxiv - Cancer Biology 2022Quote: ... The viability of the cells was then evaluated by incubating at 37 °C for 2.5 h with 200 mg/ml MTT (Calbiochem). The MTT solution was removed afterwards ...
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bioRxiv - Microbiology 2019Quote: ... while the basolateral media were replenished with the 1:1 stem cell/L-WRN media with 10μM Y-27632 (Calbiochem) and 100 to 500 ng/ml of the receptor activator of NF-κB ligand (RANKL ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated with N‐[N‐(3,5‐difluorophenacetyl)‐L‐alanyl]‐sphenylglycine t‐butyl ester (DAPT) (Calbiochem, Camarillo, CA, USA) 24 h before performing the experiment ...
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bioRxiv - Immunology 2022Quote: ... and 1 × 106 cells were treated with rapamycin (10 ng/ml, 30 min, 37°C; Calbiochem, San Diego, CA) prior to treatment with rhGH.
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then incubated in a wash buffer consisting of 2X nuclease free SSC and 10% deionized formamide (Calbiochem 4610) for 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Whole cell samples were ground in liquid nitrogen and lysed in 8 M urea supplemented with 1% protease inhibitor cocktail (Calbiochem). Lysates were centrifuged at 12,000 x g and the supernatant was quantified by BCA assays (Beyotime) ...
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bioRxiv - Biochemistry 2020Quote: ... medium was removed and cells were put on ice and immediately lysed by scraping in ice-cold lysis buffer supplemented with protease inhibitor (Calbiochem) and phosphatase inhibitor (1 mM sodium orthovanadate (NaVO3) ...
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bioRxiv - Immunology 2019Quote: Eosinophil degranulation was assessed by performing ELISA for Eosinophil Derived Neurotoxin (EDN) on cell free supernatants from eosinophils cultured for 4hrs with N-Formylmethionyl-leucyl-phenylalanine (FMLP) (Calbiochem) or cytokines ...
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bioRxiv - Systems Biology 2020Quote: ... cell lysis buffer (1X phosphate buffered saline (PBS) with 1% TrintonX-100 and 1% EDTA-free protease inhibitor (Calbiochem, #539134)) was added to each well and the plate was put on ice for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... in PBS for 10 min and the coverslips were mounted cell side down on object glasses with Mowiol (Calbiochem, 475904) containing 50 mg/ml 1,4-diazabicyclo[2.2.2]octane (Dabco ...
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bioRxiv - Cell Biology 2022Quote: ... Forty-eight hours post transfection cells were removed and incubated in 250 μL/well of HEK Lysis Buffer containing protease Inhibitor Cocktail Set V (Calbiochem) before being centrifuged at 13 000 rpm for 10 min ...
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bioRxiv - Neuroscience 2022Quote: Cultures were rinsed twice with PBS and cell membrane extracts were collected according to the method of ProteoExtract Kit (Calbiochem). Samples were heated with equal volumes of SDS sample buffer (Novex ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS-T and glass coverslips were added on cells (mitosis) or coverslips were mounted onto slides with Mowiol containing DAPI (Calbiochem).
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bioRxiv - Neuroscience 2023Quote: ... cells were washed with PBS three times and the coverslips mounted using FluorSave (Millipore Calbiochem; Cat Number: 34-578-9).
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bioRxiv - Immunology 2020Quote: ... Cell lysates were collected in RIPA buffer plus inhibitors (protease: complete mini Roche; 11836153001, and phosphatases: cocktail set II Calbiochem; 524625) and then incubated with the antibody-bead mix overnight at 4°C on a shaker ...
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bioRxiv - Neuroscience 2022Quote: ... frozen neural progenitor cells (NPCs) were thawed in the neural maintenance growth medium and supplemented with ROCK inhibitor (Y-27632; 10 mM Calbiochem, #688000). Cells were incubated in a humidified chamber at 37°C with 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then lysed in Pierce™ IP Lysis Buffer (87788, ThermoFischer Scientific, Rockford, USA) containing proteases (539138, Calbiochem, Darmstadt, Germany) and phosphatases inhibitors (524629 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were released from the arrest by transferring them to α-factor-free medium containing 150 μg/mL of Pronase (Calbiochem) and cultured for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were collected using 75μl Lysis Buffer (Cell Signaling Technology #9803, Phosphatase Inhibitor Cocktail Set II #524625 and Protease Inhibitor Cocktail Set III #539134 both Calbiochem). Upon cell lysis using a sonicator ...
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bioRxiv - Cell Biology 2023Quote: ... RPE-1 cells transfected with either siR-NAs targeting Luciferase or MiniBAR for 72 h were treated with Y27632 (Calbiochem 146986-50-7) at 50 µM for 24 h in DMEM-F12 without SVF before fixation.
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bioRxiv - Neuroscience 2023Quote: Expression of glutathione S-transferase (GST)-fused Amph1 constructs in Escherichia coli BL21 DE3 cells was induced with 1 mM isopropyl β-D-1-thiogalactopyranoside (Calbiochem, cat no. 420322) at OD600nm 0.6-0.8 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.