Labshake search
Citations for Calbiochem :
451 - 500 of 565 citations for 4 15 Oppositene 1 7 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... proteinase K was inactivated by addition of 1 mM phenylmethanesulfonyl fluoride (Calbiochem, 7110). Cells were lysed by addition of 1% Triton X-100 followed by incubation at room temperature for 15 min ...
-
bioRxiv - Biophysics 2024Quote: ... and 1:1,000 dilution of Protease Inhibitor Cocktail Set III (EDTA free, Calbiochem) supplemented with 10 mM of the detergent LMNG (Anatrace) ...
-
bioRxiv - Biochemistry 2020Quote: ... and stable pools were selected with medium containing 500 µg ml-1 G418 (Calbiochem). The expression of the indicated genes in the stable pools was induced by the addition of 1 µg ml-1 doxycycline (Sigma ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... blocked (200 μL, PBS 1% BSA wt/vol, Calbiochem #12659-100GM, 3 h, RT) and washed (3x ...
-
bioRxiv - Immunology 2020Quote: ... blocked (200 µL, PBS 1% BSA wt/vol, Calbiochem #12659-100GM, 3 h, RT) and washed (3x ...
-
bioRxiv - Physiology 2021Quote: ... 0.1% sodium dodecyl sulfate) containing protease inhibitor cocktail (Cocktail set 1, Calbiochem, Bilerica, MA) and phosphatase inhibitors (25 mM NaF ...
-
bioRxiv - Cell Biology 2021Quote: ... 3 and 6 hours with the inhibitors for ILK (1 μM CPD022, Calbiochem, #407331), FAK (5 μM FAK inhibitor 14 ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices were incubated with polyclonal anti-tyrosine hydroxylase rabbit antibodies (1:1000, Calbiochem, Merck) and were incubated overnight in carrier solution (containing in PBS ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells expressing the shRNA constructs were selected using 1 mg/mL Neomycin (Calbiochem, #480100). To induce expression of shRNAs cells were treated for 3 days with 2 µg/mL Dox.
-
bioRxiv - Microbiology 2023Quote: ... diluted 5-fold in 100% MeOH containing 1 µM glycocholic acid (Calbiochem, 100.1% pure), and centrifuged for 10 min at 4000 RPM (Thermo Sorvall ST 40R ...
-
bioRxiv - Microbiology 2024Quote: ... diluted 10-fold in 50% MeOH containing 1 µM glycocholic acid (Calbiochem, 100.1% pure), and centrifuged for 10 min at 4000 RPM (Thermo Sorvall ST 40R ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated with primary antibodies against RAD51 (rabbit anti-Rad51 1/200, Calbiochem PC130) and biotin (mouse anti-biotin 1/6,000 ...
-
bioRxiv - Neuroscience 2021Quote: ... Tissue samples were homogenized in TritonX-100 and protease inhibitor-containing (1:100, #539131, Calbiochem) Tris-HCl buffer (TBS ...
-
bioRxiv - Cell Biology 2021Quote: This study used Latrunculin-A at a final concentration of 1 µM (Calbiochem Cat# 428021), the PAK1 kinase inhibitor ...
-
bioRxiv - Neuroscience 2021Quote: ... One replicate of each sample was treated with 1 mM conduritol B epoxide (CBE, Calbiochem) for 10 min before all samples were incubated for 1 hr with 2.5 mM of the fluorescent GCase substrate methylumbillifery β-D-glucopyranoside (4MUG ...
-
bioRxiv - Biochemistry 2021Quote: ... the cells were incubated with a blocking buffer (1% (w/v) bovine serum albumin (Calbiochem) and 0.5% (w/v ...
-
bioRxiv - Immunology 2023Quote: ... Tissue samples were homogenized in TritonX-100 and protease inhibitor-containing (1:100, #539131, Calbiochem) Tris-HCl buffer (TBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... Immunodetection of RAS proteins was carried out with pan-RAS (Ab-3; Calbiochem; 1:1,000) antibodies ...
-
bioRxiv - Cell Biology 2023Quote: ... The WAPL-AID cell line was supplemented with puromycin (0.5 µg mL-1, Calbiochem, 540411). HeLa SMC4-AID_WAPL-dTAG cells were supplemented with blasticidin S (6 µg mL-1 ...
-
bioRxiv - Cell Biology 2024Quote: ... 1000 ng of previously purified IqgC [55] was added to 1 μM H-Ras (Calbiochem) as a control or to purified GST-RapA(wt ...
-
bioRxiv - Immunology 2024Quote: ... 1% Triton X-100 in the presence of Protease Inhibitor Cocktail Set III (Calbiochem®) and 0.2 mg sodium orthovanadate for 5 min on ice ...
-
bioRxiv - Molecular Biology 2024Quote: ... The resistant clone expressing LOX-1 and mutated AT1 in response to doxycycline (Calbiochem, USA) was selected for use in the experiments (CHO-LOX-1-AT1mg ...
-
bioRxiv - Neuroscience 2024Quote: ... probed with horseradish peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies (1:10,000, Calbiochem) and developed using chemiluminescence reagents (ECL ...
-
bioRxiv - Cancer Biology 2022Quote: ... ZBTB18 or ZBTB18-mut as described above or treated with 5 µM RN-1 inhibitor (Calbiochem), for 96h ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated for 1 h at room temperature with primary antibodies (rabbit anti-RAD51 Calbiochem PC130 1/200 and mouse anti-PCNA Santa Cruz PC10 1/500 ...
-
bioRxiv - Cell Biology 2021Quote: ... two other kinase inhibitors were used: 1 μM and 1.5 μM of AKT VIII (Calbiochem #124018) or 100 nM and 200 nM of trametinib (Selleckchem #GSK1120212) ...
-
bioRxiv - Neuroscience 2022Quote: ... Nuclei were counterstained with 1 µg/ml of DAPI and sections were mounted with Fluorsave (Calbiochem).
-
bioRxiv - Neuroscience 2021Quote: ... #MCA1957) and peptide-rich sensory nerve fibres with a rabbit anti-CGRP (1:2,000, Calbiochem, #PC205L). After washes ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... PamChip arrays (PamGene #88101) were blocked for 20 cycles with 1% Bovine serum albumin (Calbiochem #126609) in Tris-buffered saline (TBS) ...
-
bioRxiv - Developmental Biology 2023Quote: Cells were harvested in RIPA buffer with 1:100 protease inhibitor cocktail set I (Calbiochem, #539131) and phosphatase inhibitors (1:100 Na2Ov4 ...
-
bioRxiv - Neuroscience 2023Quote: ... protease inhibitors (PI, 1:4000 dilution of protease inhibitor cocktail 3 w/o EDTA, Calbiochem, #539134) and benzonase (1:1000 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1% Triton X-100 lysis buffer supplemented with protease inhibitors (539134, Calbiochem, San Diego, CA, USA), and collected by scrapping ...
-
bioRxiv - Biochemistry 2024Quote: ... 10 mM Imidazole (Buffer A) supplemented with 1:1000 protease inhibitors (Calbiochem Set III, Merck 539134), 3 µg/mL Benzonase-MBP ...
-
bioRxiv - Molecular Biology 2020Quote: ... Pellets were resuspended in 300 µl of DMEM 2% FBS 1 µg/mL propidium iodide (Calbiochem, 537060) and filtered through a 40-µm cell strainer (BD Falcon ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lyophilized MEISi-1 and MEISi-2 small molecules were dissolved in sterile dimethyl sulfoxide (DMSO, Calbiochem, 317275). Luciferase reporter assays were carried out as we have done previously 13,14,16,17 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The master mix recipe contained polyclonal anti-rabbit anti-MLH1 (Calbiochem, San Diego CA; diluted 1:50) or anti-rabbit anti-DMC1 (mix of DMC1) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed with ice cold PBS and lysed in IP lysis buffer (50 mM Tris, pH 7.5, 10 mM NaCl, 2.5 mM MgCl2, 1 mM DTT, 0.01% digitonin [Calbiochem 300410] and protease inhibitors ...
-
bioRxiv - Cell Biology 2023Quote: ... Controls were treated for 30 minutes with KRBH + 2.8 mM glucose + 1 μM latrunculin B (Calbiochem, #428020) or 1 μM jasplakinolide (Millipore Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... GFP-Rab11 WTlow and DNlow cell culture media was also supplemented with 1.25 μg.mL-1 puromycin (Calbiochem). Cells were regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: polyclonal rabbit anti-tyrosine hydroxylase (TH, catalog # 657012, 1:1000, Calbiochem), monoclonal mouse anti-TH (catalog # MAB318 ...
-
bioRxiv - Cell Biology 2022Quote: ... resuspended in 6 ml lysis buffer (1X PBS, 500 mM NaCl, 1% Triton X-100, lysozyme, Calbiochem EDTA-free protease inhibitor cocktail set III ...
-
bioRxiv - Microbiology 2023Quote: ... cells were pretreated 1 h at 37°C with 20 nM bafilomycin A1 (Calbiochem, stock in DMSO) to block endocytosis ...
-
bioRxiv - Neuroscience 2024Quote: ... incubated overnight in TBST with 1% bovine serum albumin (BSA; Calbiochem by Millipore Sigma, St. Louis, MO) and primary antiserum (Millipore Sigma MAB318 ...
-
bioRxiv - Biochemistry 2024Quote: ... 1 mM phenylmethane sulfonyl fluoride (PMSF) and Protease Inhibitor Cocktail Set III (Calbiochem, San Diego, CA, USA). The cells were disrupted in an EmulsiFlex C3 homogenizer (Avestin Inc. ...
-
bioRxiv - Cell Biology 2024Quote: ... along with protease inhibitors (PI, 1:750 dilution of protease inhibitor cocktail 3 w/o EDTA, Calbiochem) and benzonase (Sigma ...
-
bioRxiv - Cell Biology 2024Quote: ... along with protease inhibitor (PI, 1:1000 dilution of protease inhibitor cocktail 3 w/o EDTA, Calbiochem) and benzonase (Sigma ...
-
bioRxiv - Cell Biology 2020Quote: ... and the cells were washed with ice-cold PBS(-/-) with 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131). The PBS was aspirated ...
-
bioRxiv - Neuroscience 2020Quote: ... 14 – 17 DIV hippocampi cultures were stained live with a GluA1 antibody (GluA1 Calbiochem PC246, rabbit, 1:20) for 15 min at 37°C in a humidified chamber ...
-
bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...
-
bioRxiv - Biochemistry 2021Quote: ... Cell pellets were sonicated in 20 mM HEPES buffer pH 7.5 containing 0.25 M sucrose and 1:100 protease inhibitor cocktail set V (Calbiochem) and centrifuged at 2,000 × g for 10 min to pellet nuclear fraction ...