Labshake search
Citations for Calbiochem :
451 - 500 of 546 citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... The resistant clone expressing LOX-1 and mutated AT1 in response to doxycycline (Calbiochem, USA) was selected for use in the experiments (CHO-LOX-1-AT1mg ...
-
bioRxiv - Neuroscience 2024Quote: ... probed with horseradish peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies (1:10,000, Calbiochem) and developed using chemiluminescence reagents (ECL ...
-
bioRxiv - Cancer Biology 2022Quote: ... ZBTB18 or ZBTB18-mut as described above or treated with 5 µM RN-1 inhibitor (Calbiochem), for 96h ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated for 1 h at room temperature with primary antibodies (rabbit anti-RAD51 Calbiochem PC130 1/200 and mouse anti-PCNA Santa Cruz PC10 1/500 ...
-
bioRxiv - Cell Biology 2021Quote: ... two other kinase inhibitors were used: 1 μM and 1.5 μM of AKT VIII (Calbiochem #124018) or 100 nM and 200 nM of trametinib (Selleckchem #GSK1120212) ...
-
bioRxiv - Neuroscience 2022Quote: ... Nuclei were counterstained with 1 µg/ml of DAPI and sections were mounted with Fluorsave (Calbiochem).
-
bioRxiv - Neuroscience 2021Quote: ... #MCA1957) and peptide-rich sensory nerve fibres with a rabbit anti-CGRP (1:2,000, Calbiochem, #PC205L). After washes ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... PamChip arrays (PamGene #88101) were blocked for 20 cycles with 1% Bovine serum albumin (Calbiochem #126609) in Tris-buffered saline (TBS) ...
-
bioRxiv - Developmental Biology 2023Quote: Cells were harvested in RIPA buffer with 1:100 protease inhibitor cocktail set I (Calbiochem, #539131) and phosphatase inhibitors (1:100 Na2Ov4 ...
-
bioRxiv - Neuroscience 2023Quote: ... protease inhibitors (PI, 1:4000 dilution of protease inhibitor cocktail 3 w/o EDTA, Calbiochem, #539134) and benzonase (1:1000 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1% Triton X-100 lysis buffer supplemented with protease inhibitors (539134, Calbiochem, San Diego, CA, USA), and collected by scrapping ...
-
bioRxiv - Biochemistry 2024Quote: ... 10 mM Imidazole (Buffer A) supplemented with 1:1000 protease inhibitors (Calbiochem Set III, Merck 539134), 3 µg/mL Benzonase-MBP ...
-
bioRxiv - Molecular Biology 2020Quote: ... Pellets were resuspended in 300 µl of DMEM 2% FBS 1 µg/mL propidium iodide (Calbiochem, 537060) and filtered through a 40-µm cell strainer (BD Falcon ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lyophilized MEISi-1 and MEISi-2 small molecules were dissolved in sterile dimethyl sulfoxide (DMSO, Calbiochem, 317275). Luciferase reporter assays were carried out as we have done previously 13,14,16,17 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The master mix recipe contained polyclonal anti-rabbit anti-MLH1 (Calbiochem, San Diego CA; diluted 1:50) or anti-rabbit anti-DMC1 (mix of DMC1) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed with ice cold PBS and lysed in IP lysis buffer (50 mM Tris, pH 7.5, 10 mM NaCl, 2.5 mM MgCl2, 1 mM DTT, 0.01% digitonin [Calbiochem 300410] and protease inhibitors ...
-
bioRxiv - Cell Biology 2023Quote: ... Controls were treated for 30 minutes with KRBH + 2.8 mM glucose + 1 μM latrunculin B (Calbiochem, #428020) or 1 μM jasplakinolide (Millipore Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... GFP-Rab11 WTlow and DNlow cell culture media was also supplemented with 1.25 μg.mL-1 puromycin (Calbiochem). Cells were regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: polyclonal rabbit anti-tyrosine hydroxylase (TH, catalog # 657012, 1:1000, Calbiochem), monoclonal mouse anti-TH (catalog # MAB318 ...
-
bioRxiv - Cell Biology 2022Quote: ... resuspended in 6 ml lysis buffer (1X PBS, 500 mM NaCl, 1% Triton X-100, lysozyme, Calbiochem EDTA-free protease inhibitor cocktail set III ...
-
bioRxiv - Microbiology 2023Quote: ... cells were pretreated 1 h at 37°C with 20 nM bafilomycin A1 (Calbiochem, stock in DMSO) to block endocytosis ...
-
bioRxiv - Neuroscience 2024Quote: ... incubated overnight in TBST with 1% bovine serum albumin (BSA; Calbiochem by Millipore Sigma, St. Louis, MO) and primary antiserum (Millipore Sigma MAB318 ...
-
bioRxiv - Biochemistry 2024Quote: ... 1 mM phenylmethane sulfonyl fluoride (PMSF) and Protease Inhibitor Cocktail Set III (Calbiochem, San Diego, CA, USA). The cells were disrupted in an EmulsiFlex C3 homogenizer (Avestin Inc. ...
-
bioRxiv - Cell Biology 2024Quote: ... along with protease inhibitors (PI, 1:750 dilution of protease inhibitor cocktail 3 w/o EDTA, Calbiochem) and benzonase (Sigma ...
-
bioRxiv - Cell Biology 2024Quote: ... along with protease inhibitor (PI, 1:1000 dilution of protease inhibitor cocktail 3 w/o EDTA, Calbiochem) and benzonase (Sigma ...
-
bioRxiv - Cell Biology 2020Quote: ... and the cells were washed with ice-cold PBS(-/-) with 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131). The PBS was aspirated ...
-
bioRxiv - Neuroscience 2020Quote: ... 14 – 17 DIV hippocampi cultures were stained live with a GluA1 antibody (GluA1 Calbiochem PC246, rabbit, 1:20) for 15 min at 37°C in a humidified chamber ...
-
bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...
-
bioRxiv - Biochemistry 2021Quote: ... Cell pellets were sonicated in 20 mM HEPES buffer pH 7.5 containing 0.25 M sucrose and 1:100 protease inhibitor cocktail set V (Calbiochem) and centrifuged at 2,000 × g for 10 min to pellet nuclear fraction ...
-
bioRxiv - Biochemistry 2021Quote: ... Data were normalized to maximal inhibition determined by 1 μM decanoyl-Arg-Val-Lys-Arg-chloromethylketone (Calbiochem #344930).
-
bioRxiv - Neuroscience 2021Quote: ... or P5–P6 DRG rat neurons were diluted to 35,000 cells/ml in Sato’s media and treated with either 1 mM dbcAMP (Calbiochem), mixed population or recombinant Histones (1-20µg/ml ...
-
bioRxiv - Immunology 2020Quote: ... Cells were grown at 37°C to OD = 0.8 and expression was induced with 0.3 mM IPTG (Isopropyl b-D-1-thiogalactopyranoside, Calbiochem) overnight at 18°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated for 1 h with the ADAM17 inhibitor Batimastat (BB-94) at 10 μM (196440, Calbiochem), with the selective ADAM10 inhibitor GI254023X (GI ...
-
bioRxiv - Molecular Biology 2024Quote: ... The pellet was suspended in 2 ml of lysis/binding buffer (25 mM HEPES/KOH, pH 7.6, 10 % glycerol, 150 mM KCl, 1 mM dithiothreitol, 0.1 % Elugent (Calbiochem), loaded into SHREDDER tubes (Pressure Biosciences Inc ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... sections were incubated with rabbit anti-c-Fos antibody diluted with the blocking solution (1:20000, #PC38, Calbiochem) for 2 days at 4°C ...
-
bioRxiv - Pathology 2024Quote: ... 2% DDM: 10µl/mg flies) supplemented with 1:1000 (v/v) Protease Inhibitor Cocktail Set III (Calbiochem, USA). Rat anti-HRP-conjugated-HA (3F10 ...
-
bioRxiv - Biophysics 2024Quote: ... Cell pellet was resuspended then resuspended in phosphate-buffer saline (PBS) containing 1 mM phenylmethylsulfonyl fluoride (PMSF) (Calbiochem), 50 μg/mL DNase I (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL, Millipore Calbiochem) or 10 uM TGF-β inhibitors (SB431542 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then harvested using lysis buffer (50mM HEPES pH7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, protease inhibitor cocktail I [Calbiochem) and centrifuged at 14,000rpm for 10 minutes ...
-
bioRxiv - Cancer Biology 2022Quote: ... collected and lysed in lysis buffer RGMT (50mM HEPES pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, plus protease inhibitor cocktail I [Calbiochem]). Cellular lysates were cleared by centrifugation and 225µL of total protein extract was incubated with biotin-tagged CIGB-300 (100μM ...
-
bioRxiv - Molecular Biology 2022Quote: ... The cell pellet was lysed at 0.5 × 109 cells/ml in ice-cold lysis buffer (0.1 mM TLCK, 1 μg/ml Leupeptin, 1x Phosphatase Inhibitor Cocktail II tablet (Calbiochem), 1 mM PMSF ...
-
bioRxiv - Developmental Biology 2022Quote: ... Tissues were homogenized in cold lysis buffer (0.1% NP-40, 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml ...
-
bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.5 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
-
bioRxiv - Biochemistry 2023Quote: ... Ubiquitin was expressed as described above except that the 2 L culture was induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) for four hrs at 37℃ ...
-
bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
-
bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
-
bioRxiv - Molecular Biology 2022Quote: ... pellets were resuspended in 800 μl of ice-cold wash buffer (50 mM NaH2PO4, 300 mM NaCl, 0.05% Tween, 1:200 diluted protease inhibitor cocktail set III (Calbiochem)) supplemented with 0.1 U/μL of recombinant RNase inhibitor (wash buffer-RIn ...
-
bioRxiv - Neuroscience 2024Quote: ... 1 mM Na3VO4 and 500 nM Cantharidin (#3322.1 Roth)] and commercial protease inhibitors (protease inhibitor cocktail III, #539134, Calbiochem). Phosphatase inhibitors were omitted when Lambda phosphatase treatment was required ...
-
bioRxiv - Molecular Biology 2023Quote: Whole-cell protein extracts were prepared using hot lysis buffer (1%SDS, 5mM EDTA, 50mM Tris, pH7.5) supplemented with protease inhibitor mixture (Calbiochem). Protein samples were diluted and boiled in 5X protein loading buffer (10% SDS ...
-
bioRxiv - Cell Biology 2022Quote: ... alone bound to glutathione sepharose 4B beads in 0.7 ml of binding buffer (1X PBS, 150 mM NaCl, 1% Triton X-100, Calbiochem EDTA-free protease inhibitor cocktail set III ...