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Citations for Leica :
1 - 50 of 59 citations for palmitic acid ethoxylated phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... gravid day 1 adults animals were picked onto prepared palmitic acid plates and plates were imaged at 2 and 5 hours at 1.6x on a stereomicroscope (Leica M165 FC) using a 1.3 MP CMOS camera (Thorlabs DCC1645C ...
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bioRxiv - Cell Biology 2020Quote: ... acid alcohol (Leica) for 5 s ...
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bioRxiv - Physiology 2023Quote: ... After fixation in 4% buffered formalin (Neutral Phosphate Buffered Formalin Fixatives, Leica, Germany), cells were permeabilized in 0.25% Triton X-100 and blocked in Sea Block Blocking Buffer (1 h ...
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bioRxiv - Neuroscience 2020Quote: ... Spain) in 0,1 M phosphate-buffered saline (PBS) and sectioned using a vibratome (Leica VT 1000S ...
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bioRxiv - Neuroscience 2023Quote: ... under constant cooling with sterile phosphate buffered saline and visualisation through a surgical microscope (Leica). In a subset of animals ...
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bioRxiv - Neuroscience 2021Quote: ... Sagittal brain sections of 50µm were collected in 0.1M phosphate buffer using a vibratome (Leica VT1000S). After sectioning ...
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bioRxiv - Neuroscience 2021Quote: ... washed in phosphate buffer saline (PBS) and sectioned (100 μm) coronally using a vibratome (Leica VT1000s). Brain sections were mounted on glass slides ...
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bioRxiv - Neuroscience 2021Quote: ... washed in phosphate buffer saline (PBS) and sectioned (300 μm) coronally using a vibratome (Leica VT1000s). To perform the measurements in the hippocampus and in the striatum ...
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bioRxiv - Physiology 2022Quote: ... Obtained conceptuses were immediately fixed in 10% buffered formalin (Neutral Phosphate Buffered Formalin Fixatives, Leica, Germany) or frozen in liquid nitrogen and stored at −80 °C ...
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bioRxiv - Neuroscience 2023Quote: ... washed in phosphate buffer saline (PBS) and sectioned (50 µm) coronally using a vibratome (Leica VT1000s). Brain sections were incubated in blocking buffer containing ...
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bioRxiv - Neuroscience 2024Quote: ... washed in phosphate buffer saline (PBS) and sectioned (50 µm) coronally using a vibratome (Leica VT1000s). For immunohistochemistry ...
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bioRxiv - Neuroscience 2023Quote: ... Brain slices (thickness 100 μm) were prepared under phosphate-buffered saline using a vibratome (Leica VT1000 S) and imaged using a fluorescence microscope (Axio Imager 2 ...
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bioRxiv - Neuroscience 2024Quote: ... Brain slices (thickness 100 µm) were prepared under phosphate-buffered saline using a vibratome (Leica VT1000 S) and imaged using a fluorescence microscope (Axio Imager 2 ...
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bioRxiv - Neuroscience 2020Quote: ... washed in phosphate buffered saline and sectioned along the axial plane (100 μm sections) using a vibratome (Leica VT1000s ...
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bioRxiv - Neuroscience 2024Quote: ... washed briefly in phosphate buffer and 100 µm-thick sections were collected with a vibratome (Leica VT1200 S). Sections were stained with nuclear staining DAPI (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... dissolved in 0.1M phosphate-buffered saline (PBS) and sectioned in 80μm thick frontal sections using a vibratome (Leica VT 1000S ...
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bioRxiv - Genomics 2024Quote: ... The nuclei were differentiated with mild acid treatment (Leica Biosystems 3803598), followed by “bluing” through exposure to a weakly alkaline solution (Leica Biosystems 3802918 ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.1M phosphate buffered saline (PBS, Fisher #BP399-20) at +4°C before slicing on a freezing microtome (Leica) in a 1:12 series of 40 µm thick coronal sections ...
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bioRxiv - Plant Biology 2021Quote: ... resin sections were stained with 0.05 % toluidine blue in 0.1 M 6.8 pH phosphate buffer (O’Brien et al., 1964) and visualized in a Leica DM 5000B microscope (Leica Microsystems) under bright field.
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bioRxiv - Neuroscience 2020Quote: ... they were washed with phosphate buffered saline (PBS) and then 50 μm thick sliced with a vibratome (Leica VT1200S).
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bioRxiv - Neuroscience 2023Quote: ... The tissue block was then rinsed in 0.1M phosphate buffer (PB) and sectioned at 50 µm thickness via vibratome (VT 1000P, Leica). Tissue sections were stored in a cryoprotectant solution at −30°C until processed for HPF ...
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bioRxiv - Neuroscience 2023Quote: ... 50µm thick coronal sections were prepared in 1x phosphate buffered saline (PBS; Fisher BP399) using a vibratome (Leica VT1000S). All sections containing hippocampus were then mounted for fluorescent imaging with DAPI (SouthernBiotech DAPI Fluoromount-Gm ...
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bioRxiv - Neuroscience 2024Quote: ... sunk in 30% (wt/vol) sucrose in phosphate buffered saline (PBS) and sectioned (50 μm) coronally (Freezing Microtome, Leica). Free-floating sections were permeabilized/blocked with 5% normal goat serum in PBS with 0.2% Triton X-100 (PBST ...
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bioRxiv - Neuroscience 2024Quote: ... myoinositol (3) and ascorbic acid (0.5) using a vibratome (Leica VT1200, Germany). Brains slices were transferred to an interface type holding chamber filled with 28 °C carbogen saturated ASCF containing (in mM) ...
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bioRxiv - Genomics 2021Quote: ... USA) and 30 µm sections cut into phosphate-buffered saline (pH 7.4) using a vibratome (Leica VT1000S Vibratome, Wetzlar, Germany). The sections were examined using bright field microscopy on a BX53 microscope fitted with a UPlanfl N 20x/0.5 objective and coupled to a SC180 camera with cellSens standard software (v 2.3 ...
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bioRxiv - Neuroscience 2022Quote: ... Brain slices (thickness 50 µm or 100 µm) were prepared under phosphate-buffered saline using a vibratome (Leica VT1000 S) and imaged using a fluorescence microscope (Axio Imager 2 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were cryoprotected with 30% sucrose in 0.1 M phosphate buffer to prepare 50 μm-thick cryosections on a cryostat (CM1900; Leica Microsystems). All immunohistochemical incubations were performed at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Spain) in 0,1 M phosphate-buffered saline (PBS) and sectioned using a vibratome (Leica VT 1000S, Leica Microsystems GmbH, Germany) on the frontal ...
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bioRxiv - Developmental Biology 2023Quote: ... Fifty-micrometer-thick coronal sections of the brains were cut in sodium phosphate buffer (PBS 50 mM, pH7.4) using a Leica VT1000S vibratome (Leica Biosystems) and stored at -20°C in cryoprotectant until further processing ...
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bioRxiv - Neuroscience 2023Quote: Brain tissues after electrophysiology brain slice preparation were fixed in 4% PFA in 0.1 M phosphate buffer overnight at 4 °C and then were resected into 70–100 μm slices using a VT1000s vibratome (Leica Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Blocks were rinsed in 0.01M phosphate buffer saline (PBS) and sliced at 50µm in the parasagittal plane with a vibratome (Leica VT-1000S). Slices selected for immunolabeling were photobleached at 4°C for 3-4 days to reduce autofluorescence.
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bioRxiv - Neuroscience 2024Quote: ... 50 μm thick transverse coronal sections of the brain were cut in sodium phosphate buffer (PBS; 50 mM, pH 7.4) using a vibratome (Leica VT1000S) and stored at -20°C in cryoprotectant (ethylene glycol:glycerine:PBS 30:30:40 vol:vol:vol ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were removed and placed in additional 10% formalin phosphate until sectioning into 40 µM thick sections via cryostat (CM 3050s; Leica Biosystems). Brain sections were stained with cresyl violet in order to visually confirm tetrode recording sites.
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Grid cells perform path integration in multiple reference frames during self-motion-based navigationbioRxiv - Neuroscience 2023Quote: ... The brains were washed with phosphate-buffered saline and cut into 50 µm thick sagittal slices with a vibratome (Leica, Germany). Cresyl violet staining was performed ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The other hemibrain was drop-fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) and sectioned (30 μm) with a sliding microtome (Leica SM2000R). All mouse experiments were performed in accordance with European Union Council Directive 86/609/EEC ...
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bioRxiv - Neuroscience 2023Quote: ... titrated to pH 7.4 with concentrated hydrochloric acid) using a VT1200s vibrating microtome (Leica). The transcardial perfusion procedure with 30 ml of chilled NMDG-HEPES aCSF was performed on P60 animals prior brain dissection and slicing ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were then transferred to 1x phosphate-buffered saline (PBS) and 35 µm thick coronal sections were cut using a vibratome (Leica, VT 1000s). Sections were collected in PBS containing 0.2% sodium azide and stored at 4°C until further processed ...
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bioRxiv - Neuroscience 2024Quote: ... dissolved in 0.1M phosphate-buffered saline (PBS) and sectioned in 80μm thick frontal sections using a vibratome (Leica VT 1000S; Leica Microsystems GmbH, Germany). The sections were collected in 4°C PBS and then processed for conventional in situ hybridization or fluorescent in situ hybridization combined with single immunofluorescence.
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bioRxiv - Neuroscience 2020Quote: ... washed in phosphate buffered saline and sectioned along the axial plane (100 μm sections) using a vibratome (Leica VT1000s, Leica Microsystems, Wetzlar, Germany). Cell bodies were stained using NeuroTrace (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: Temporal lobe tissue fixed with 2.5% glutaraldehyde-2% PFA in 0.1 M phosphate buffer (PB) was transversely sectioned at 200 µm using a Leica VT1200S vibratome (Leica Microsystems GmbH, Heidelberg, Germany). Slices were further post-fixed in 2% osmium tetroxide in 0.1 M PB for 1.5 hours at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by decalcification of bone in a formic acid solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a Leica ASP6025 tissue processor ...
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bioRxiv - Molecular Biology 2021Quote: ... and after several washes in phosphate-buffered saline (PBS) and cryo-preservation in 30% sucrose in PBS were embedded in cryomedium (Surgipath Cryogel®, Leica Microsystems, Vienna, Austria). Subsequently ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were rinsed 3 times in phosphate buffered saline and 50 μM coronal brain slices were obtained using a vibratome (Leica VT1000, Buffalo Grove, Il), without embedding the tissue ...
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bioRxiv - Neuroscience 2024Quote: ... They were then perfused transcardially with ice-cold (4°C) phosphate buffered saline (PBS) using the Perfusion Two™ system (Leica Biosystems, Chicago, IL).
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bioRxiv - Microbiology 2022Quote: ... femur and tibia were decalcified in a formic acid and formaldehyde solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a tissue processor (Leica ASP6025 ...
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bioRxiv - Bioengineering 2021Quote: ... The uptake of the fatty acids was monitored in real-time using a fluorescence microscope with incubation (Leica DMi8 with incubation unit ...
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bioRxiv - Biochemistry 2021Quote: ... proteins fixed in 30% ethanol/15% acetic acid and the gel piece embedded in tissue embedding media (Leica). After careful mounting on a cryo-holder ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were rinsed in tap water and dipped 3-5 times in 0.5% Acid Alcohol (Leica Biosystems 3803651), followed by rinsing in tap water and bluing with Scott’s Tap Water (Electron Microscopy Sciences 2607007 ...
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bioRxiv - Microbiology 2024Quote: ... and tibia were decalcified in a formic acid and formaldehyde solution (Surgipath Decalcifier I, Leica Biosystems, Wetzlar, Germany). All fixed tissues were then processed in ethanol and xylene in a tissue processor and embedded in paraffin (Leica ASP6025 ...
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bioRxiv - Cell Biology 2024Quote: Brightfield images (H&E, Acid phosphatase) were collected via a DM4000B upright microscope (Leica Microsystems, Milton Keynes, UK) using either a 10x or 20x objective (HC PL FLUOTAR PH1/PH2 ...