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1 - 50 of 525 citations for pLenti CMV TO Puro DEST 670 1 since 2020

• CLCd and CLCf act redundantly at the TGN/EE and prevent acidification of the Golgi stack

  Stefan Scholl, et al., bioRxiv - Plant Biology 2021

  Quote: ... between 620-670 nm for mRFP and between 670-750 nm for FM4-64 using hybrid detectors (HyD; Leica Microsystems GmbH, Wetzlar, Germany). Images for non-quantitative purposes were adjusted in brightness and contrast using Fiji (Schindelin et al. ...
• INTS13 Mutations Causing a Developmental Ciliopathy Disrupt Integrator Complex Assembly

  Lauren G. Mascibroda, et al., bioRxiv - Biochemistry 2020

  Quote: ... A tunable (470-670 nm) pulsed white light laser (Leica WLL) and a UV 405 nm diode laser was used for excitation ...
• Phosphorylation-dependent routing of RLP44 towards brassinosteroid or phytosulfokine signalling

  Borja Garnelo Gómez, et al., bioRxiv - Plant Biology 2021

  Quote: ... at 545-573 nm for YFP and 620-670 nm for RFP or mCherry using HyD hybrid detectors (Leica) or photomultipliers (PMT ...
• The cell fate controlling CLE40 peptide requires CNGC9 to trigger highly localized Ca²⁺ transients in Arabidopsis thaliana root meristems

  Maike Breiden, et al., bioRxiv - Plant Biology 2020

  Quote: ... The Ca2+ sensor R-GECO1 was excited with a white light laser at 561 nm and its emission was detected at 590 nm to 670 nm using a HyD detector (Leica) and an EMCCD iXon Ultra detector (Zeiss) ...
• The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway

  Nils Stührwohldt, et al., bioRxiv - Plant Biology 2020

  Quote: ... Emission was detected at 500-550 nm for sfGFP and 610-670 nm for mRFP/mCherry with HyD hybrid detectors (Leica Microsystems) in standard operation-mode ...
• Non-canonical function of ADAM10 in presynaptic plasticity

  Julia Bär, et al., bioRxiv - Neuroscience 2024

  Quote: A Leica TCS SP8-3X gatedSTED system equipped with a 470 to 670 nm pulsed white light laser (WLL) and a 100x objective (Leica, HC APO CS2 100x/1.40 oil) was used for confocal and gated STED imaging ...
• Three-axis classification of mouse lung mesenchymal cells reveals two populations of myofibroblasts

  Odemaris Narvaez del Pilar, Jichao Chen, bioRxiv - Developmental Biology 2021

  Quote: ... mouse anti-NK2 homeobox 1 (NKX2-1, 1:500, Leica Biosystems, TTF-1-L-CE), goat anti-interleukin 33 (IL33 ...
• Morphogenic, molecular, and cellular adaptations for unidirectional airflow in the chicken lung

  Kamryn N Gerner-Mauro, et al., bioRxiv - Developmental Biology 2024

  Quote: ... mouse anti-NK2 homeobox 1 (NKX2-1, 1:500, TTF-1-L-CE, Leica Biosystems); Sambucus Nigra Lectin (SNA ...
• Development of novel in vitro human alveolar epithelial cell models to study distal lung biology and disease

  Evelyn Tran, et al., bioRxiv - Cell Biology 2020

  Quote: ... NKX2-1 (Leica TTF-1-L-CE), Ki67 (Abcam ab16667) ...
• High expression of VRT2 increases the number of rudimentary basal spikelets in wheat

  Anna E. Backhaus, et al., bioRxiv - Plant Biology 2022

  Quote: ... and basal sections (1:1:1 ratio) using a stereo microscope (Leica MZ16). Sections were immediately placed into 96-well plates (on ice ...
• TRAIL agonists rescue mice from radiation-induced lung injury

  Jillian Strandberg, et al., bioRxiv - Pathology 2023

  Quote: ... Diluted primary antibody (CST #8242 1:800; CST #99940, 1:150; CST #9718, 1:500; Abcam ab107099, 1:X; Leica P53-CM5P-L 1:200) was applied and slides were incubated at 4°C overnight ...
• Behavior- and circuit-specific cortico-striatal decoupling during the early development of Parkinson’s disease-like syndrome

  Xu-Ran Yao, Yang Liu, Wei-Tong Zheng, Kai-Wen He, bioRxiv - Neuroscience 2024

  Quote: ... 20 μm coronal slices were sectioned using the cryostat (Leica, CM3050s-1-1-1). These sections were incubated in blocking solution (PBS containing 5% normal goat serum (Sigma ...
• Tissue-resident FOLR2⁺ macrophages associate with tumor-infiltrating CD8⁺ T cells and with increased survival of breast cancer patients

  Rodrigo Nalio Ramos, et al., bioRxiv - Immunology 2021

  Quote: ... anti-CD31 (clone PECAM-1, 1:50, Leica) and anti-TREM2 ...
• DNA damage response at telomeres boosts the transcription of SARS-CoV-2 receptor ACE2 during aging

  Sara Sepe, et al., bioRxiv - Cell Biology 2021

  Quote: ... mouse monoclonal TTF-1 (clone SPT24, 1:100 pH6, NCL-L-TTF-1, Leica Novocastra), rabbit polyclonal anti-53BP1 (1:1000 ...
• ERBB signalling contributes to immune evasion in KRAS-driven lung adenocarcinoma

  Sarah Laing, et al., bioRxiv - Cancer Biology 2023

  Quote: ... F4/80 (Abcam ab6640, 1:100, Enzyme 1 Leica), CD45R (Abcam ab64100 ...
• Co-editing PINK1 and DJ-1 genes via AAV-delivered CRISPR/Cas9 system in adult monkey brains elicits classic Parkinsonian phenotypes

  Hao Li, et al., bioRxiv - Neuroscience 2020

  Quote: ... Coronal sections of the whole SN of Young 1 and Old 1 were obtained by sectioning the tissue (20 μm) on a freezing microtome (Leica, CM1850UV-1-1, Germany) at −24 °C.
• Nerve fibers in the Tumor Microenvironment are co-localized with Tertiary Lymphoid Structures

  Lara R. Heij, et al., bioRxiv - Cancer Biology 2020

  Quote: ... CD21 (DAKO, 1:25, CD23 (Leica, CD23-1B12, 1:50), CD4 (DAKO ...
• ERBB signalling contributes to immune evasion in KRAS-driven lung adenocarcinoma

  Sarah Laing, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and Ly6G (2B Scientific BE0075-1, 1:60000, ER2 Leica). Nkp46 and FoxP3 were stained on a Dako AutostainerLink48 ...
• A 4D transcriptomic map for the evolution of multiple sclerosis-like lesions in the marmoset brain

  Jing-Ping Lin, et al., bioRxiv - Neuroscience 2023

  Quote: ... PV Poly-HRP Anti-Mouse IgG (Leica, PV6119, 1:1), ImmPRESS®-AP Horse Anti-Rabbit IgG Polymer (Vector ...
• Phenotyping the virulence of SARS-CoV-2 variants in hamsters by digital pathology and machine learning

  Gavin R Meehan, et al., bioRxiv - Microbiology 2023

  Quote: ... TTF-1 (Leica), IBA1 (Fujifilm) ...
• Comprehensive epigenomic profiling of human alveolar epithelial differentiation identifies key epigenetic states and transcription factor co-regulatory networks for maintenance of distal lung identity

  Beiyun Zhou, et al., bioRxiv - Genomics 2021

  Quote: ... AT2 cell purity was determined by NKX2-1 cytospin staining (1:100, Leica Biosystems, Cat # NCL-1-TTF1). Donor1 was 83% positive ...
• CXCR2 inhibition enables NASH-HCC immunotherapy

  Jack Leslie, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Ly6G (clone 1A8, 2B Scientific BE0075-1, ER2 Leica, 1:60000), LTF (Thermofisher ...
• WNT/β-catenin dependant alteration of cortical neurogenesis in a human stem cell model of SETBP1 disorder

  Lucia F. Cardo, Meng Li, bioRxiv - Developmental Biology 2021

  Quote: ... 1/1000 (Leica biosystems), Rabbit anti-PH3 ...
• Lung epithelial progenitor-mediated release of TGF-β regulates induction and persistence of lung CD8⁺ T_RM cells following mucosal BCG vaccination

  Judith A. Blake, et al., bioRxiv - Immunology 2024

  Quote: ... and #1 coverslips (Leica).
• Spatiotemporal map of the developing human reproductive tract at single-cell resolution

  Valentina Lorenzi, et al., bioRxiv - Developmental Biology 2024

  Quote: ... Aqueous eosin 1% (Leica) was manually applied onto sections with a pipette and rinsed with deionised water after 1-3 seconds ...
• Gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium

  Riikka Mäkilä, et al., bioRxiv - Plant Biology 2022

  Quote: ... and then incubated for 1 h in a 1:1 solution of 100% ethanol and solution A (Leica Historesin Embedding kit). After 2 h in solution A ...
• The transcription regulator Lmo3 is required for cell fate specification in the external globus pallidus

  Shiona Biswas, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse anti-TTF1 (Nkx2.1) (1:100; Novocastra; Leica Biosystems NCL-L-TTF-1), mouse anti-NeuN (1:500 ...
• Monocytes Mobilized by Gut Neurons Remodel the Enteric Nervous System

  Sravya Kurapati, et al., bioRxiv - Immunology 2024

  Quote: ... ýIII-Tubulin (1:250) and human HLA-DR (1:250, LN-3, Leica/Novocastra) followed by staining with a cocktail of DAPI and fluorochrome-conjugated secondary antibodies and mounting on histological slides.
• Finite element model of the non-keratinized buccal tissue under the impact of negative pressure

  David Klein Cerrejon, Daniel Gao, David Sachs, bioRxiv - Bioengineering 2024

  Quote: ... Images were detected by monochrome DFC365FX digital camera (12 bits, 1×1 BIN, Leica microsystems ...
• Characterization of highly proliferative decidual precursor cells during the window of implantation in human endometrium

  Maria Diniz-da-Costa, et al., bioRxiv - Cell Biology 2020

  Quote: ... CD56 (1:200, Leica Biosystems), or CD163 (1:200 ...
• mRNA stability fine tunes gene expression in the developing cortex to control neurogenesis

  Lucas D. Serdar, et al., bioRxiv - Neuroscience 2024

  Quote: ... p53 (Leica CM5, 1:250), CC3 (CST 9661S ...
• Langerhans islets induce anti-tumor immunity at the expense of glycemic control and predict chemotherapy response in pancreatic cancer

  Azaz Ahmed, et al., bioRxiv - Cancer Biology 2021

  Quote: ... CD20 (1:100, HIER 1, mouse monoclonal, clone L26, Leica, Germany; CD20-L26-L-CE), CD163 (1:500 ...
• Langerhans islets induce anti-tumor immunity at the expense of glycemic control and predict chemotherapy response in pancreatic cancer

  Azaz Ahmed, et al., bioRxiv - Cancer Biology 2021

  Quote: ... CD8 (1:50, HIER 1, mouse monoclonal, clone 4B11, Leica, Germany, CD8-4B11-L-CE), CD20 (1:100 ...
• PME-mediated pectin modifications promote haustoria initiation and xylem bridge development in the parasitic plant Phtheirospermum japonicum

  Martina Leso, et al., bioRxiv - Plant Biology 2023

  Quote: ... 100% ethanol) and incubated overnight in a 1:1 solution of 100% ethanol:Historesin solution (Leica). The solution was exchanged with Historesin and the samples incubated again overnight at 4°C ...
• Single Cell Resolution Spatial Mapping of Human Hematopoiesis Reveals Aging-Associated Topographic Remodeling

  Aleksandr Sarachakov, et al., bioRxiv - Systems Biology 2023

  Quote: ... in a sequential order as shown: 1) Opal 480/CD71 (1:80, 10F11, Leica concentrate), 2 ...
• Langerhans islets induce anti-tumor immunity at the expense of glycemic control and predict chemotherapy response in pancreatic cancer

  Azaz Ahmed, et al., bioRxiv - Cancer Biology 2021

  Quote: ... CD4 (1:100, HIER 1, mouse monoclonal, clone 4B12, Leica, Germany; CD4-368-L-CE-H), CD8 (1:50 ...
• A suspension technique for efficient large-scale cancer organoid culturing and perturbation screens

  Stacey Price, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and p53 (D07, 1:50, Leica) were applied with negative controls as previously described2.
• Gene regulatory networks underlying human microglia maturation

  Claudia Z. Han, et al., bioRxiv - Genomics 2021

  Quote: ... mouse Ki67 (1:1000, Leica, ACK02), rabbit Lyve1 (1:200 ...
• LARP1 regulates metabolism and mTORC1 activity in cancer

  James Chettle, et al., bioRxiv - Cancer Biology 2022

  Quote: ... ER Solution 1 (AR9961, Leica Biosystems) was used ...
• N-terminal tags impair the ability of Lamin A to provide structural support to the nucleus

  Jacob Odell, Jan Lammerding, bioRxiv - Cell Biology 2024

  Quote: ... Emerin (Leica Emerin-NCL, 1:1000). DAPI was added 1:1000 in PBS for 15 minutes ...
• Integrative multiomic approaches reveal ZMAT3 and p21 as conserved hubs in the p53 tumor suppression network

  Anthony M. Boutelle, et al., bioRxiv - Cancer Biology 2024

  Quote: ... p53 (CM5, 1:1000 Leica Novocastra), p21 (EPR18021 ...
• OrBITS: Label-free and time-lapse monitoring of patient derived organoids for advanced drug screening

  Christophe Deben, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Slides were stained manually with mouse anti-TTF1 (clone SPT24; TTF-1-L-CE, Leica, 1/400, 25’) and mouse anti-P40 (clone BC28 ...
• Cancer cell-specific MHCII expression as a determinant of the immune infiltrate organization and function in the non-small cell lung cancer tumor microenvironment

  Amber M. Johnson, et al., bioRxiv - Cancer Biology 2021

  Quote: ... CD3 (clone LN10, dilution 1:500, Leica), CD14 (clone SP192 ...
• Ageing-associated myelin dysfunction drives amyloid deposition in mouse models of Alzheimer’s disease

  Constanze Depp, et al., bioRxiv - Neuroscience 2021

  Quote: ... anti-GFAP (mouse, GA5, Leica, 1:200), anti-n-terminal APP (22c11 ...
• Reciprocal Priming between Receptor Tyrosine Kinases at Recycling Endosomes Orchestrates Cellular Signalling Outputs

  Michael P. Smith, et al., bioRxiv - Cell Biology 2021

  Quote: ... for 1 hour and visualised by Leica Sp8 inverted confocal microscopy in serial sections of 20 μm ...
• Skeletal Muscle Stem Cells Modulate Niche Function in Duchenne Muscular Dystrophy through YY1-CCL5 Axis

  Yang Li, et al., bioRxiv - Cell Biology 2024

  Quote: ... eMyHC (Leica NCL-MHC-d; 1:200) for staining of muscle cryosections ...
• Kinesin family member Kif23 regulates cytokinetic division and maintains neural stem/progenitor cell pool in the developing neocortex

  Sharmin Naher, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-p53 (1:1000; Leica Biosystems), rabbit-anti-active caspase-3 (1:1000 ...
• An immunohistochemical atlas of necroptotic pathway expression

  Shene Chiou, et al., bioRxiv - Cell Biology 2024

  Quote: ... Epitope Retrieval Solution 1 (Leica Cat#AR9961), Epitope Retrieval Solution 2 (Leica Cat#AR9640) ...
• Fgf9-Nolz-1-Wnt2 Signaling Axis Regulates Morphogenesis of the Lung

  Shih-Yun Chen, Fu-Chin Liu, bioRxiv - Developmental Biology 2022

  Quote: ... mouse anti-Ki67 (1:500, Novocastra/Leica), rabbit anti-PH3 (1:1000 ...
• Nuclear m6A Reader YTHDC1 Promotes Muscle Stem Cell Activation/Proliferation by Regulating mRNA Splicing and Nuclear Export

  Yulong Qiao, et al., bioRxiv - Molecular Biology 2022

  Quote: ... eMyHC (Leica NCL-MHC-d;1:200), laminin (Sigma-Aldrich L9393 ...