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Citations for Leica :
401 - 450 of 498 citations for hsa mir 33a 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... GFP signals were detected at different time intervals between 48-96 h post infiltration (hpi) with the TCS SP8 microscope (Leica).
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bioRxiv - Cell Biology 2023Quote: ... Phase-contrast images were recorded over 20 hours with a time lapse of 15 minutes using a Microfluor inverted microscope (Leica) with a 10× objective and equipped with an incubation system set to 5% CO2 and 37°C ...
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bioRxiv - Developmental Biology 2024Quote: All experiments of time-lapse microscopy of growing embryos were recorded under a bright-field microscope (Leica DMI600 B, Olympus CKX41 or Leica DMi8 inverted phase contrast microscopes ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were washed three times in PBS and sectioned (75-100 μm thickness) in a VT1000 Vibratome (Leica Biosystems). Tissue sections were incubated in antigen retrieval solution (10 mM sodium citrate ...
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bioRxiv - Cell Biology 2024Quote: Total fluorescence intensities were measured in injected cells as well as in the two neighbouring cells on each side (for cell identification, see Fig. 3a) in the time-lapse image series described above (Leica LAS X ...
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bioRxiv - Cancer Biology 2021Quote: ... At least ten images were taken per well at each time point to calculate the width of scratched area using LAS X software (Leica Microsystems). The cell migration percentage was calculated using the following formula ...
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bioRxiv - Developmental Biology 2021Quote: ... The time-lapse movies were taken from 34 hpf on a Leica SP8 confocal microscope equipped with HyD detectors (Leica Microsystems) using a 20x (NA 0.5 ...
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bioRxiv - Neuroscience 2020Quote: ... was introduced at an impact depth of 0.5 mm with a 2-mm diameter round impact tip (3.0 m/s speed and 100 ms dwell time) by using an electromagnetically driven CCI device (Impact One™ Stereotaxic CCI Instrument, Leica). After injury ...
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bioRxiv - Plant Biology 2019Quote: ... Embryos were individually imaged at the time of transfer and again 72h later using a LEICA M205 FA microscope fitted with a DFC 550 camera (LEICA Instruments). Relative embryo expansion rates over that 72 h interval were calculated from measurements of projected areas using ImageJ software.
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bioRxiv - Neuroscience 2019Quote: ... Brains were then rinsed with PBS five times (10 min each) and sectioned coronally at 60 μm using a Leica VT1000S vibratome (Leica, Germany).
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bioRxiv - Plant Biology 2020Quote: ... Seven-day-old Arabidopsis seedlings were incubated in DMSO or 50 μM DNP for indicated times and then rapidly frozen with an HPM100 high-pressure freezer (Leica Microsystems). The samples were freeze-substituted at −80°C for 72 h ...
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bioRxiv - Neuroscience 2019Quote: ... bright-field photomicrographs of 15 wild-type larvae across three clutches were taken at each developmental time-point using an 8 megapixel iSight camera (Apple) through the ocular of a stereoscope (M80, Leica Microsystems). Larvae were immobilized dorsal up in 2% lowmelting temperature agar (Thermo Fisher Scientific 16520) ...
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bioRxiv - Systems Biology 2021Quote: ... were loaded onto a flat specimen carrier (200 μm deep) and frozen multiple times in a Leica EM PACT2 high-pressure freezer (Leica Microsystems). Frozen specimens were transferred into glass bottles with screw caps containing 2% (wt/vol ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed again three times with PBS before mounting coverslips with ImmunoMount (Epredia) and read on a confocal fluorescence microscope (Leica TCS SP8, Leica Microsystems).
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bioRxiv - Microbiology 2020Quote: ... At various time points expanding colonies were imaged using a M125C stereo microscope equipped with a DFC310FX digital camera (Leica Microsystems) and images processed with Fiji ...
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bioRxiv - Cell Biology 2022Quote: ... and healing by cell migration was monitored for 24 hours using time-lapse microscopy via an inverted microscope (DMIRE2; Leica Microsystems) with a motorized stage and live-cell apparatus (37 °C humidified chamber with 5% CO2 ...
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bioRxiv - Genetics 2023Quote: Fluorescence images (dye assay) and time-lapse movies (IFT movies) were captured using an epi-fluorescence upright microscope (Leica DM6 B) (3 frames per second ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were then washed two times for 8 min in DPBS and mounted on slides (Leica Surgipath X-tra Adhesive, 3800202) using Vectashield Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... We imaged 20 to 25 fields of views per well and focal points were manually set in each position before starting the acquisition and kept constant during the whole time-lapse thanks to the Adaptive Focus Control (AFC, Leica Microsystems). The total duration of the time-lapse on non-adherent cells was 72 hours ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were washed three times with ice-cold PBS and observed with a confocal laser-scanning microscopy (Leica SP8, Wetzlar, Germany). At least three fields of view were analyzed ...
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bioRxiv - Biophysics 2019Quote: ... Then a bulk of muscle was washed 2 to 3 times in cold PBS and fixed on the specimen holder of VT1200S vibrating blade microtome (Leica Biosystems, US). The buffer tray of microtome was ice bathed and filled with cold PBS while slicing ...
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bioRxiv - Microbiology 2021Quote: ... either 1 μm sections or time-lapse movies were taken with a spinning disc confocal system (Intelligent Imaging Innovations) mounted on an inverted microscope (Leica DMIRE2; Leica) using the 100×1.4 NA oil objective ...
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bioRxiv - Cell Biology 2019Quote: ... either 0.6 μm sections or time-lapse movies were taken with a spinning disc confocal system (Intelligent Imaging Innovations) mounted on an inverted microscope (Leica DMIRE2; Leica) using the 63 × or the 100 × 1.4 NA oil objective ...
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bioRxiv - Cancer Biology 2019Quote: ... Larvae were washed 3 times with PBS 1x and livers were carefully dissected with the use of forceps on a stereomicroscope (Leica MZ 9.5). After dissection ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed a couple of times with water before they were visualized under the microscope (Leica DM IRB, TV camera). Five randomly fields were chosen to capture images using the ZEISS Efficient Navigation ...
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bioRxiv - Neuroscience 2022Quote: ... Fixed brains were washed three times for 5 min with PB and 150 µm-thick coronal sections were cut with a vibratome (Leica Microsystems, VT1000S). Tissue sections were labeled with DiIC18 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The chamber plates were washed with PBS (three times) and then images were taken with a confocal laser scanning microscope Leica TCS SP8 STED 3X (Leica microsystems, Germany) using the following lasers (i ...
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bioRxiv - Biochemistry 2024Quote: ... The grids were then back blotted a second time before plunge freezing in liquid ethane using a Leica EM GP2 (Leica Microsystems, Germany)
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bioRxiv - Microbiology 2021Quote: ... The cell morphology was determined by analysis of at least 100 cells per time point on a Leica DM650 microscope (Leica Biosystems, Wetzlar, Germany).
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bioRxiv - Bioengineering 2020Quote: The influence of GO concentration on the structure of the 3D bioprinted scaffolds over time was observed using a Zoom stereomicroscope (Leica Microsystems, Heerbrugg, Switzerland). Two-dimensional top view images of the 3D bioprinted acellular scaffolds (n = 3 ...
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bioRxiv - Neuroscience 2021Quote: Experiments with full-length PSD-95 were performed using the time domain analysis (TCSPC) method with a Leica DMR TCS SP2 AOBS on an inverted stand (Leica Microsystems, Mannheim, Germany). The pulsed light source was a tunable Ti:Sapphire laser (Chameleon ...
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bioRxiv - Biochemistry 2022Quote: ... at least 30 cells of each experimental condition were tracked semi--+automatically in the time-lapse movies using Track Objects plug-in in Leica MM AF powered by MetaMorph® software (Leica Microsystems GmbH). Cells dividing or colliding with other cells were excluded from the analysis ...
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bioRxiv - Neuroscience 2020Quote: ... 1.0 mm deep with a controlled velocity of 5.0+0.2 m/s and a dwell time of 100 ms using a stereotaxic electromagnetic impactor (Impact one, Leica; Buffalo Grove, IL, USA) equipped with a 5.0 mm flat steel tip ...
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bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Immunology 2020Quote: Time-domain FLIM experiments on live TZM-bl cells expressing Laconic were performed using a Time Correlated Single Photon Counting (TCSPC) approach operated by the FALCON module (Leica Microsystems, Manheim, Germany) integrated within the Leica SP8-X-SMD microscope ...
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bioRxiv - Microbiology 2022Quote: ... back-blotted for 6-8 s after a 30-s pre-blotting time and immediately plunge frozen into liquid ethane using a GP-EM2 (Leica Microsystems, Wetzlar, Germany) with a chamber maintained at 20 °C and 95% humidity ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The morphologies of the spheroids were captured at various time points using an inverted light microscope equipped with a digital camera (Leica DM-IL MC170).
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bioRxiv - Cell Biology 2024Quote: Total fluorescence intensities were measured in injected cells as well as in the two neighbouring cells on each side (for cell identification, see Fig. 3a) in the time-lapse image series described above (Leica LAS X, Leica Microsystems, Wetzlar, Germany). Periods for quantitative analysis were selected in which the fluorescence intensity in the impaled cell had not reached a plateau yet ...
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bioRxiv - Microbiology 2021Quote: ... spore number in 0.1-μL samples was counted 300 times for the first experiment and 100 times for the second and the third experiments under a Leica DM500 microscope (Leica Microsystems Canada, Concord, ON), with 12 μL sample applied on the chamber for each counting.
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed again three times with PBS before mounting coverslips with ImmunoMount (Epredia) and read on a confocal fluorescence microscope (Leica TCS SP8, Leica Microsystems).
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bioRxiv - Neuroscience 2021Quote: ... Brains were rinsed 3 times in phosphate buffered saline and 50 μM coronal brain slices were obtained using a vibratome (Leica VT1000, Buffalo Grove, Il), without embedding the tissue ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were washed three times with PBS 1x and the BBB-oC was observed by confocal microscopy (Leica TCS SP5 Multi-photon system) at 63x.
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed an additional 3 times in 100% ethanol for 15 minutes each and dried using a Leica EM CPD030 critical point dryer (Leica Microsystems, Buffalo Grove, IL) with CO2 as the transitional medium ...
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bioRxiv - Neuroscience 2019Quote: ... A Leica Refine-HRP kit (Leica Biosystems Newcastle Ltd. ...
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bioRxiv - Developmental Biology 2021Quote: ... Electroporation efficiency and extent of overexpression were evaluated at the time of embryo collection by detecting GFP and RFP on a stereo dissecting microscope under epifluorescent illumination (MZFLIII-TS, Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Microbiology 2020Quote: ... Time-lapse imaging of the samples was performed on a Leica TCS SP5 confocal microscope using a 20x objective (Leica HC PL APO, NA=0.7) or 40x (Leica HC PL APO ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were washed three times with PBS 1x and the microdevices were inspected by confocal microscopy at 63x (Leica TCS SP5 Multi-photon system). Fiji/ImageJ® software was then employed to treat the acquired images.
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bioRxiv - Microbiology 2024Quote: ... Time-lapse imaging was performed on a Leica TCS SP5 confocal microscope with a 40x oil immersion objective (Leica HC PL APO CS2, NA=1.3) 5 min after addition of 4 mM EGTA ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were then washed 2 times for 8 minutes in DPBS containing 0.3% Triton X100 and mounted on slides (Leica Surgipath® X-tra™ Adhesive, 3800202) using Vectashield Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... or Bond proteolytic enzyme kit (AR9551, Leica). Thereafter ...