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Citations for Leica :
51 - 100 of 106 citations for bta mir 486 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... three sets of N ≥ 8 per genotype were pinched and scored for open wounds under a fluorescent stereo microscope (Leica MZ16FA with Planapo 1.6x objective and appropriate filters) ...
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bioRxiv - Biochemistry 2020Quote: ... all worms were sorted manually using a fluorescence microscope (Leica M205 with the GFP fluorescence filter set, Leica Microsystems, Germany). The inclusion criteria being the most significant observable fluorescence of worms in a population defined as “bright” and insignificant observable fluorescence defined as “dim” ...
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bioRxiv - Cell Biology 2021Quote: ... specific band-pass filter sets were used to prevent bleed through from one channel to the next (for GFP, L5 (Leica); for mCherry ...
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bioRxiv - Cell Biology 2021Quote: ... Live cells with positive DCF staining were imaged with a filter set appropriate for fluorescein (FITC) using a fluorescence microscope (Leica).
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bioRxiv - Neuroscience 2021Quote: ... we set up a transmission microscope composed of a high NA collimator objective (LEICA HC PL APO 40×/1.1 W) on a 5-axis kinematic mount ...
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bioRxiv - Microbiology 2020Quote: ... berghei was analysed by reflection contrast polarized light microscopy using a Leica DMR widefield microscope equipped with a ProgRes MF camera (Jenoptik) and the POL filter set 513813 (Leica). Hz from P ...
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bioRxiv - Cell Biology 2021Quote: ... The tubing was set to allow pumping of the solutions through the heart (attached to a perfusion needle (Leica, 39471024)) into the vasculature with the same entry point used for PBS and PFA perfusion steps described above ...
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bioRxiv - Neuroscience 2022Quote: ... Cyan light activation was provided with a metal halide FLU03 lamp on a Leica FLUO3 upright microscope using an EGFP filter set (Leica) at fixed 2.5x zoom ...
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bioRxiv - Biophysics 2023Quote: ... The tile sets were merged and maximum intensity projections for each channel were calculated using LAS X (Leica, Wetzlar, Germany). Q35-YFP was excited at 488 nm and 0.5 % total laser power and emission was detected from 509 nm to 551 nm ...
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bioRxiv - Neuroscience 2023Quote: Confocal images were obtained using a Leica TCS SP8 system with HC PL APO 20x/0.75 IMM CS2 multi-immersion objective set to glycerol (Leica Systems). Z-stacks were obtained at 1.0μm depth intervals with sequential acquisition settings of 1024 x 1024 pixels ...
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bioRxiv - Microbiology 2023Quote: ... using a custom microscope set-up (Visitron Systems, Puchheim, Germany) based on a Leica DMI 6000 B inverse microscope (Leica) equipped with a pco.edge sCMOS camera (PCO) ...
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bioRxiv - Neuroscience 2024Quote: ... The STG was then illuminated with a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems, 3-7 min). The neurons were considered completely photoinactivated when the membrane potential reached 0 mV ...
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bioRxiv - Microbiology 2020Quote: ... for 1h at RT before being imaged on an SP8 Lightning laser scanning microscope (Leica) with a HC PL APO CS2 40x/1.30 lens.
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bioRxiv - Cancer Biology 2022Quote: ... 8 μm FFPE sections were cut using a microtome (Leica RM2125 RT, Leica Biosystems, Germany) and fixed onto frosted micro slides (Blue Star ...
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bioRxiv - Cancer Biology 2022Quote: ... 8 μm FFPE sections were cut using a microtome (Leica RM2125 RT, Leica Biosystems, Germany) and fixed onto frosted micro slides (Blue Star ...
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bioRxiv - Bioengineering 2022Quote: ... embedded in paraffin and then sliced on a microtome Leica RM2125 RT (Leica Biosystems, Germany). The haematoxylin and eosin (H&E ...
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bioRxiv - Developmental Biology 2023Quote: ... Kidneys blocks were sectioned at 5-7 µm thickness using a microtome (Leica; RM2125 RTS), placed onto microscope slides (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... brains were flash frozen using crushed dry ice and coronal sections (40 μm) containing the BNST were collected using a cryostat set to −20° C (Leica Microsystems). Sections were wet-mounted onto gelatin-subbed glass microscope slides ...
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bioRxiv - Biochemistry 2020Quote: ... and allowed to set O/N at 4 °C before being imaged using a SP8 white-laser confocal microscope (Leica Microsystems). Filters were adjusted specifically for each stain to visualize nuclei (Hoechst ...
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bioRxiv - Bioengineering 2020Quote: ... The diffusion experiments were performed in the environmentally controlled chamber (set at 37 °C, 5% CO2) of a fluorescent microscope (Leica, CTR6500). The dextran solution was perfused inside the microvessel channel and images were acquired every 30 sec over 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... One full set of cresyl violet stained slides for each animal was scanned using the Aperio ScanScope whole slide scanner (Leica Biosystems) with the 20x objective ...
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bioRxiv - Biochemistry 2020Quote: ... Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems). Images containing complexes were collected manually with a 120 kV TALOS transmission electron microscope (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV.
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV ...
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bioRxiv - Neuroscience 2023Quote: ... the first was set to circulate the solution through the heart into the vasculature by using a perfusion needle (Leica, 39471024), the second was immersed into the solution chamber where the animal was placed ...
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bioRxiv - Bioengineering 2019Quote: ... through DIC and FITC (488 nm Ex/525 nm Em) filter sets on a Leica DM16000 microscope (Leica Microsystems, Buffalo Grove, IL) using the 10X objective ...
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bioRxiv - Neuroscience 2021Quote: ... The STG or stn near its entrance to the STG was then illuminated for 3-7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica, Wetzlar, Germany). Complete photoinactivation of LP was confirmed when the membrane potential reached 0 mV ...
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bioRxiv - Plant Biology 2022Quote: ... Embedded samples were then sectioned into 10 μm-thick sections using RM2125 RTS microtome (Leica, Germany) with TC-65 tungsten blade.
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bioRxiv - Immunology 2021Quote: ... through LEICA BOND RX 1h room- temperature (RT) and developed with Bond Polymer Refine Detection (Leica, DS9800). For hematoxylin and eosin (H&E ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were mounted after the final PBSTx washes (RT) and analysed with a MZ16F fluorescence stereomicroscope (Leica) equipped with a ProgRes C3 camera (Jenoptik ...
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bioRxiv - Biophysics 2023Quote: ... FFPE tissue samples were cut using a ultramicrotome in 2 πm thick sections (RM2125 RTS, Leica Biosystems, Germany).
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bioRxiv - Developmental Biology 2020Quote: ... Whole embryo live imaging of Moe-ABD-GFP was performed with a Leica TCS SP8 confocal system at RT with a 40X NA 1.4 objective (Leica), and 300nm step sizes.
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bioRxiv - Microbiology 2021Quote: ... The movies were recorded at room temperature (RT) using the Leica software (LAS X version 1.1.0.12420; Leica Microsystems, Wetzlar). Maximum projections of the microscopy movies were generated using Fiji software (67 ...
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bioRxiv - Microbiology 2020Quote: ... washed with PBS and mounted on agarose-coated microscope slides for analysis at RT using a Leica DMI600B epifluorescence system (equipped with a Leica CRT6000 illumination system ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues were washed and incubated for 1h RT with the primary antibody diluted in IHC Antibody Diluent (Novocastra, Leica RE7133). Subsequently ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were then cryoprotected in 10% and 30% sucrose solutions for 2 hrs at RT and finally incubated in 30% sucrose and tissue embedding medium (Leica) (1:1 ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were cured at RT overnight and imaged using brightfield microscopy (DM4000B upright microscope) in Leica Application Suite software version 2.6 (Leica Microsystems). Negative control images for immunohistochemistry are provided in Figure S2.
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bioRxiv - Cell Biology 2022Quote: ... Slides were cured at RT overnight and imaged using brightfield microscopy (DM4000B upright microscope) in Leica Application Suite software version 2.6 (Leica Microsystems).
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bioRxiv - Cancer Biology 2023Quote: ... 5µm thick sections were cut using a Leica RM2125 RTS microtome and sectioned onto Poly-L-lysine coated slides (Leica Surgipath Apex Superior adhesive slides ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
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bioRxiv - Bioengineering 2021Quote: ... Sections were incubated (30 min, room temperature (rt)) with an anti-GFP antibody (see Table S2) in Leica Primary Antibody Diluent (Leica Biosystems). Bond Polymer Refine Detection (Leica Biosystems ...
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bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
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bioRxiv - Immunology 2022Quote: ... they were paraffin-embedded and sectioned (7 µm thickness) on slides using a Leica RM2125 RTS microtome (Leica Biosystems, Concord, Canada). Slides were deparaffinized and washed using two rounds of toluene (T324-1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μL of cell pellets were transferred onto copper carrier grids and subjected to high-pressure freezing using a Leica EMPACT2 high-pressure freezer + Rapid Transfer System (RTS) (Leica Microsystems)52 ...
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bioRxiv - Cancer Biology 2023Quote: ... Formalin-Fixed Paraffin-Embedded blocks containing tumor or matched normal patient samples were cut into sections of 4μm thickness using a microtome (Leica, HistoCore RM2125 RTS) (Leica, Wetzlar, Germany). The tissue sections were processed as previously described (Bala et al. ...
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bioRxiv - Microbiology 2024Quote: ... cells were visualized for 15 min at 20 sec intervals at RT using a Leica DMi8 inverted microscope with a Leica DFC9000 GT camera and using the LASX software (Leica Microsystems). Individual cells were tracked using Metamorph 7.5 (Molecular Devices ...
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bioRxiv - Plant Biology 2022Quote: ... high-light illumination of the cells was achieved by exposing the cuvette to white light set to an intensity of 620 μmol photons m-2 s-1 (Leica KL1500 LCD, peak 648 nm, FWHM 220 nm), chosen as the minimal light level necessary to saturate photosynthetic reactions and induce qE activity at the onset of illumination ...
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bioRxiv - Cancer Biology 2023Quote: ... Formalin-Fixed Paraffin-Embedded blocks containing tumor or matched normal patient samples were cut into sections of 4μm thickness using a microtome (Leica, HistoCore RM2125 RTS) (Leica ...