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Citations for Leica :
151 - 200 of 314 citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... the sectioned samples were placed on a Hematoxylin and Eosin (H&E) automated staining platform (Leica Auto Stainer XL, Leica Biosystems) without applying any heat during the staining process ...
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bioRxiv - Bioengineering 2023Quote: ... the sectioned samples were placed on a Hematoxylin and Eosin (H&E) automated staining platform (Leica Auto Stainer XL, Leica Biosystems) without applying any heat during the staining process ...
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bioRxiv - Neuroscience 2019Quote: ... The position of the microelectrode was controlled by a Leitz micromanipulator (Model M; Leica Microsystems, Wetzlar, Germany). Electrode depth was monitored with a digimatic indicator (ID-C125MB ...
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Multiplex-GAM: genome-wide identification of chromatin contacts yields insights not captured by Hi-CbioRxiv - Molecular Biology 2021Quote: ... collected in sucrose droplets (2.1 M in PBS) and transferred to steel frame PEN membrane slides (Leica) UV treated for 45 min prior to use ...
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bioRxiv - Cancer Biology 2022Quote: ... H&E stainings were performed in Tartu University Hospital by pathologists using Leica staining automat and ST Infinity H&E Staining System (Leica, cat. 38016998). Stained lung sections were scanned using a slide scanner (Leica SCN400 ...
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bioRxiv - Pathology 2020Quote: Four representative sections from each aortic and heart graft were stained with hematoxylin and eosin (H&E; Leica Biosystems, Buffalo Grove, IL) or Movat’s pentachrome (Newcomer Supply ...
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bioRxiv - Cell Biology 2022Quote: ... The frozen tissue was sectioned to 7-10 µm using a 2800 Frigocut E cryo-microtome (Reichert-Jung; Leica Instruments, Nussloch, Germany). Sections of the native tissue were fixed in 1% FA (in PBS) ...
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bioRxiv - Cell Biology 2023Quote: ... Semithin sections (250 nm) were cut from conventionally embedded vestibular organs on an Ultracut E or an UC7 ultramicrotome (Leica Microsystems, Germany) with a 35° diamond knife (Diatome AG ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were heated at 60°C for 1 h in an oven before H&E staining in the autostainer (Leica Autostainer XL). IHC was performed using a detection kit (Vector Laboratories ...
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bioRxiv - Plant Biology 2019Quote: ... the detection of protein–protein interaction by BiFC was performed using confocal microscopy (Leica SP5 CLSM). The excitation wavelength was 514 nm (argon laser ...
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bioRxiv - Bioengineering 2023Quote: ... Nonspecific protein-protein interactions were blocked with Leica PowerVision IHC/ISH Super Blocking solution (Leica PV6122) at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... the GFP-LC3 and LAMP1-RFP dot formations were detected under a fluorescence microscope (DMi8-M, Leica, German). The cell containing ≥ 5 GFP-LC3 dots is defined as autophagy positive cells (Mizushima et al. ...
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bioRxiv - Plant Biology 2020Quote: ... For the SF-M the walnut was kept in the cryostat microtome (CM3050 S, Leica Biosystems, Nussloch, Germany) for 1-4h (depending on the nut size ...
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bioRxiv - Neuroscience 2023Quote: Brains were cut into 200 µm thick coronal slices containing M1 (AP: 0.00 – 0.60 mm from Bregma) on VT1000S vibrating blade microtome (Leica). Slices were immediately transferred to the incubation chamber containing carbogenated ...
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bioRxiv - Genetics 2021Quote: FFPE tissue sections were cut using a microtome and baked at 62°C for 20 minutes followed by haematoxylin and eosin (H&E) staining using a Leica Autostainer XL (Leica Microsystems, Wetzlar, Germany). Slides were mounted with DPX (distyrene ...
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bioRxiv - Immunology 2019Quote: ... eight histological sections from central region of the each alveolar socket were stained with Picrosirius Red stain and the images of 4 histological fields a 40× objective were captured by a polarizing lens coupled to a binocular inverted microscope (Leica DM IRB/E), as previously described [55] ...
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bioRxiv - Plant Biology 2020Quote: Roots of one-week-old Arabidopsis lines transformed with CcAOS-eGFP or with the corresponding empty vector were observed in a Leica SP-E confocal microscope (Leica Microsistemas, Carnaxide, Portugal) with the settings described below and the 488nm laser line.
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bioRxiv - Neuroscience 2021Quote: ... The brain tissue was then embedded in OCT medium and sliced to 14 μm thick sections on a cryostat (Leica HM 505 E). Tissue sections were collected directly onto SuperFrostPlus slides and stored at −80 deg for up to 3 months until RNA in situ hybridization for EYFP (virally driven) ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... GAS samples were weighed then separated into small fiber bundles using fine forceps and a Leica S4 E surgical dissecting microscope (Leica Microsystems, Wetzlar, Germany). Muscle fiber bundles were incubated for 30 min at low rocking speed in glass scintillation vials containing buffer A supplemented with 0.05 mg/mL saponin to selectively permeabilize the sarcolemma ...
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bioRxiv - Molecular Biology 2022Quote: Wound healing response at day 7 and scar tissue morphology and ECM architecture at day 30 post-wounding were determined by hematoxylin and eosin (H&E) staining and Masson’s Trichrome Staining respectively (Leica Biosystems, Buffalo Grove, IL). Epithelial gap ...
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated and subsequently stained with hematoxylin and eosin (H&E) and appropriate antibodies (Supplementary Table 3) using the automated system BOND RX (Leica Biosystems, Newcastle, UK). Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030, Leica Microsystems, Wetzlar, Germany). Using a scoring system bleeding intensity was evaluated in vaginal smears by microscopy ...
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bioRxiv - Neuroscience 2020Quote: ... we sliced the brain into 100 µm sections and imaged GFP and/or tdTomato fluorescence from every other slice using a 10x/0.4NA objective (Leica). Anatomical landmarks ...
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bioRxiv - Neuroscience 2021Quote: ... Coronal slices (300 μm thick) between 2.6 and 3.5 mm posterior to bregma were prepared using a vibrating blade microtome (Leica VT1200S). Slices were kept submerged in artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Biophysics 2021Quote: ... plunge-frozen in liquid nitrogen in 2.1 M sucrose and semi-thin sections (350 nm) were cut using the cryo ultramicrotome (UC6, Leica). Sections were picked up with a custom made metal loop in a droplet of 1% methylcellulose and 1.15 M sucrose and transferred to 35 mm glass bottom dishes (MatTek ...
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bioRxiv - Biophysics 2021Quote: ... plunge-frozen in liquid nitrogen in 2.1 M sucrose and semi-thick sections (400 nm) were cut using the cryo-ultramicrotome (UC6, Leica). Sections were picked up with a custom made metal loop in a droplet of 1% methylcellulose and 1.15 M sucrose and transferred to 35 mm glass bottom dishes (MatTek ...
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bioRxiv - Cell Biology 2022Quote: ... At least 20 cells/condition were imaged on either ELYRA PS.1 SIM/PAL-M LSM780 (Zeiss) using Plan-Apochromat 40x /1.4 oil immersion objective or DMi8 (Leica) with Lumencor SOLA SE led light (Lumencor ...
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bioRxiv - Bioengineering 2019Quote: ... with a 20 µm objective aperture diameter and a working distance at / around 2.5 mm (GeminiSEM 500, Zeiss; Cryo station VCT100, Leica). A sublimation step at −90°C was applied for 15 min in the SEM chamber to remove ice ...
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bioRxiv - Genetics 2021Quote: ... Animals stained were mounting in agarose 2 X pads with a sodium azide drop (0,05 M) to count amyloid deposits by fluorescent microscopy (DM2500, Leica) and quantify stained area of worms by digital dissection microscope (DMD108 ...
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bioRxiv - Plant Biology 2021Quote: ... resin sections were stained with 0.05 % toluidine blue in 0.1 M 6.8 pH phosphate buffer (O’Brien et al., 1964) and visualized in a Leica DM 5000B microscope (Leica Microsystems) under bright field.
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bioRxiv - Plant Biology 2023Quote: ... The samples were washed with 0.05 M sodium cacodylate buffer and embedded in 4% agarose and sliced using VT1000S vibratome (Leica). For localization and auxin/cytokinin distribution pattern ...
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bioRxiv - Microbiology 2021Quote: ... H&E stained tissues were imaged first using a Leica DMI100 Brightfield Microscope and DMC5400 20 MP color CMOS camera (Leica Microsystems, Concord, Ontario, Canada). Further imaging was done using a Aperio ScanScope XT on select tissues ...
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bioRxiv - Cell Biology 2022Quote: ... One 5um section from each tissue type was stained with hematoxylin and Eosin (H&E) and scanned at 20X magnification using an Aperio AT2 (Leica Biosystems, Buffalo Grove, IL) into whole slide digital images.
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bioRxiv - Cancer Biology 2023Quote: ... The protein-protein signal was scanned and scored digitally by a pathologist with Aperio image scope software (Leica Microsystems).
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bioRxiv - Cancer Biology 2021Quote: ... Macroscopic images of unpeeled tissue were obtained under Leica M80 zoom stereomicroscope with Leica Plan 1.0x Objective M-Series 10450167 coupled with Leica DFC295 Camera (Leica microsystems). Macroscopic tumors were punched and flash frozen in tissue freezing medium (Leica catalog no ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were cryoprotected with 30% sucrose in 0.1 M PB to prepare 50 μm-thick cryosections on a cryostat (CM1900; Leica Microsystems). All immunohistochemical incubations were performed at room temperature ...
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bioRxiv - Plant Biology 2020Quote: Plasmolysis was induced by mounting 6-day-old seedlings in 0.8 M mannitol on microscope slides and directly observed using confocal microscopy (Leica SP8). The proportion of the cell wall length in direct contact with the plasma membrane marker SYP122-mCitrine after plasmolysis was measured using Fiji after plasmolysis ...
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bioRxiv - Neuroscience 2020Quote: ... The immunofluorescent staining was performed on 40-µm thick free-floating coronal midbrain slice containing VTA and SNc DA neurons that were prepared using a vibratome (Leica VT1200S ...
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bioRxiv - Neuroscience 2022Quote: Images were collected from 2-3 consecutive sections 144 µm apart containing the central part of the demyelinating lesion using a TCS STED CW SP8 super-resolution microscope (Leica) and imported into NIH ImageJ software ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were cryoprotected with 30% sucrose in 0.1 M phosphate buffer to prepare 50 μm-thick cryosections on a cryostat (CM1900; Leica Microsystems). All immunohistochemical incubations were performed at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... and the area was calculated at 14 dpi using a Leica M 165C stereomicroscope equipped with Leica LASv4.3 image analysis software (Leica Microsystems). At 42 dpi ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was thoroughly washed in 0.1 M PBS and thereafter sectioned (40 µm thickness) using a vibratome (Leica VT1000, Leica Microsystems). The sections were stored in 1x PBS at 4°C.
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bioRxiv - Neuroscience 2020Quote: ... Pulled glass pipette (tip inner diameter of 5–15 µm) filled with a tracer was stereotaxically inserted in the brain using Angle One stereotaxic instrument (myNeuroLab.com; currently Leica Instruments) and a small amount of the tracer (5–300 nL ...
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bioRxiv - Neuroscience 2020Quote: ... Spain) in 0,1 M phosphate-buffered saline (PBS) and sectioned using a vibratome (Leica VT 1000S, Leica Microsystems GmbH, Germany) on the frontal ...
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bioRxiv - Genomics 2021Quote: ... Sections of 10μm thickness were cut from the paraffine-embedded tissue blocks and mounted onto poly-ethylene naphtholate (PEN)-membrane slides (Leica). Tissue slides were stained with the following haematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2023Quote: ... 12μm cryosections (DRGs) and 18-20 µm cryosections (SC, skin, E15 and P0) were obtained using a standard cryostat (Leica).
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bioRxiv - Neuroscience 2022Quote: ... brains were rapidly removed and coronal slices (300 μm) containing the right AC were prepared in a cutting solution at 1 °C using a Vibratome (VT1200 S; Leica). The cutting solution ...
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bioRxiv - Neuroscience 2022Quote: ... the brain was cut into 2 mm thick coronal sections in 0.15 M cacodylate buffer using a vibratome (Leica VT1200). Then ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 μm/pixel) were captured from 100 to 300 frames through an objective lens (HCX IRAPOL 25×, NA = 1.00, Leica Microsystems) at a rate of 2.5 s per frame (i.e. ...
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bioRxiv - Neuroscience 2023Quote: ... it was washed three times with 0.1 M PB and cut along the sagittal plane using a vibratome (Leica VT2100S). Serial parasagittal sections (50 mm thick ...