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Citations for Leica :
301 - 327 of 327 citations for Water Nuclease Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Free floating 60 μ parasagittal sections of brain and pituitary were made with a vibratome (VT1000S Leica, Wetzlar, Germany). Tissue slices were incubated with hybridization probes for 18 h at 55°C and then incubated with sheep anti-DIG and anti-FITC antibodies conjugated with peroxidase (POD ...
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bioRxiv - Neuroscience 2019Quote: ... 40-µm free-floating tissue cryosections were collected on a Leica CM3050-S cryostat (Leica Biosystems, Buffalo Grove, IL). Tissue for in situ hybridization was cryosectioned at 14-μm and collected onto Superfrost Plus slides ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then cut into 50 µm-thick free-floating coronal sections using a Leica VT1200S vibratome (Leica Biosystems) and stored long-term at 4 °C in DPBS with 0.1 % Sodium Azide.
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bioRxiv - Neuroscience 2023Quote: Free floating sections (40 µm) of brain and lumbar spinal cord were obtained using a Vibratome (Leica VT 1200S). Sections were mounted on positively charged slides and dried overnight at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... Laser scanning is performed using a pair of galvanometric mirrors (Cambridge Instruments).The beam is expanded using a telescope to overfill the objective back aperture (Leica 63x, 1.2 NA, water immersion) and is directed into the objective using a dichroic mirror (Semrock FF685-DI02-25X36) ...
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bioRxiv - Neuroscience 2020Quote: ... The immunofluorescent staining was performed on 40-µm thick free-floating coronal midbrain slice containing VTA and SNc DA neurons that were prepared using a vibratome (Leica VT1200S ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sagittal sections (40 μm) of the entire MEC were cut using a vibratome (VT 1200S, Leica, Wetzlar, Germany). Every fourth ection was collected for IHC ...
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bioRxiv - Neuroscience 2020Quote: ... In short: tissue blocks were sectioned at 10 μm in an RNAse free cryostat and mounted on PEN-slides (Leica). Immediately after mounting ...
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bioRxiv - Neuroscience 2022Quote: ... the perfused and postfixed brains were equilibrated in RNase-free 30% sucrose and then cut into 40-μm-thick coronal sections on a cryotome (Leica CM300, Leica). Floating brain sections were placed in chamber slides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Sagittal free-floating sections (30mm) were cut around the region of substantia nigra using a cryostat (Leica CM 3050S, Germany) and ...
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bioRxiv - Neuroscience 2021Quote: ... microglial density quantifications and Iba-1:P2RY12 or Iba-1:TMEM119 colocalization analysis 40 µm free-floating sections were cut with a sliding microtome (Leica) and stained with relevant antibodies in 10% normal goat serum (NGS ...
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bioRxiv - Physiology 2020Quote: ... Symbiotic acantharians (with algal endosymbionts) and free-living Phaeocystis cordata in culture were cryofixed using high-pressure freezing (HPM100, Leica), in which ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating sections with a vibrating microtome (Leica). Brain sections were kept at −20°C in a solution containing 300 mL.L−1 ethylene glycol ...
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bioRxiv - Microbiology 2022Quote: ... Cells were kept in serum- and phenol red-free MEM containing 1% glucose during the analysis (Leica Biosystems 6000B microscope). A total of 15 random snapshots (55-70 cells in total ...
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bioRxiv - Biochemistry 2023Quote: ... The fresh Phenol Red-free DMEM media with 10% FBS was added prior to confocal microscopic experiment (Leica Stellaris 8). A 405 nm laser excitation was used to image BFP and a 488 nm laser excitation was used to image EGFP.
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bioRxiv - Cell Biology 2020Quote: ... The light is focused onto a small confocal volume through a 63x water immersion objective lens with NA=1.2 (Leica HC PL APO 63x/1.20 W CORR). The signal is detected by sensitive detectors (Leica HyD SMD ...
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bioRxiv - Neuroscience 2021Quote: ... The change in the intensity of the Ca2+indicator is quantified to estimate the change in the concentration of free Ca2+ using calcium imaging software suite (Leica Systems) to record fluorescence emission at alternating excitation wavelengths of 357 and 380 nm ...
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bioRxiv - Neuroscience 2021Quote: ... brains were sectioned sagittally at 35 μm thickness as free-floating sections with a Leica 9000s sliding microtome (Leica, Wetzlar, Germany). For immunofluorescence staining ...
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bioRxiv - Neuroscience 2020Quote: Fixed brains were cut into three series of 50μm free-floating sections using a Vibratome 1000Plus (Leica Microsystems, Buffalo Grove, IL) and stored at −20°C in cryoprotectant (0.1M potassium phosphate buffer ...
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bioRxiv - Immunology 2023Quote: ... from Apoe-/-LysMCre and Apoe-/- LysMCreCnr1flox/flox male mice collected after 4 weeks WD were mounted on RNase-free membrane metal frame slides (POL FrameSlide, Leica Microsystems). Following deparaffinization under RNase-free conditions ...
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bioRxiv - Neuroscience 2023Quote: ... the brains were sectioned into free-floating coronal sections with a thickness of 50 μm using a Leica VT1200S vibratome (Leica Biosystems). Coronal sections ranging from -1.34 mm to -2.18 mm from the bregma were selected from each mouse ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating coronal sections with a vibrating microtome (Leica, VT1000). All the following steps were done with gentle shaking ...
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bioRxiv - Developmental Biology 2019Quote: ... and reporter activities from 56 hpf to 144 hpf was performed using a Leica DMi8 combined with a CSU-X1 (Yokogawa) spinning at 10 000 rpm, 2 simultaneous cameras (TuCam Flash4.0, Hamamatsu) and a water immersion objective (Leica 20X, N.A. 0.75 or Leica 40X, N.A. 1.1). 1ms exposure was used for red blood cells imaging and 20ms exposure for valve structure and reporter activity experiments ...
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bioRxiv - Neuroscience 2022Quote: ... the perfused and postfixed brains were equilibrated in RNase-free 30% sucrose and then cut into 40-μm-thick coronal sections on a cryotome (Leica CM300, Leica). Floating brain sections were placed in chamber slides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... The agar embedded sections were sliced into 40 μm-thick free-floating sections slices on a Leica VT1000 S Vibratome (Leica, Shanghai, China) for immunofluorescent staining ...
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bioRxiv - Neuroscience 2020Quote: ... The immunofluorescent staining was performed on 40-µm thick free-floating coronal midbrain slice containing VTA and SNc DA neurons that were prepared using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL). For identification of DAT-specific deletion of PERK and consequential reduction of phosphorylated eIF2α (p-eIF2α ...
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bioRxiv - Neuroscience 2020Quote: ... Extracted brains were then sectioned into 50 μm-thick free-floating coronal slices using a semi-automatic vibratome (VT1200, Leica Biosystems, Wetzlar, Germany). Slices were stored in PBS at 4 °C ...