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Citations for Leica :
1 - 50 of 1130 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... a hybrid detector system (Leica HyD) and the Leica Application Suite LAS X software ...
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bioRxiv - Molecular Biology 2022Quote: ... respectively with two separate Hybrid GaAsp detectors in sequential mode (Leica microsystem, Germany). Data for FLIM were acquired until at least 1500 counts were collected in the brightest pixel of the image ...
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bioRxiv - Cell Biology 2023Quote: ... A 680 nm short pass filter and a 560 nm long pass dichroic mirror were used to respectively reduce the laser scattering and to split the NAD(P)H and FAD fluorescence toward two hybrid photomultiplier detectors (HyD, Leica Microsystems). 460/60 nm ...
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bioRxiv - Biochemistry 2024Quote: ... equipped with hybrid detectors (Leica HyD) and the following diode lasers ...
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bioRxiv - Cell Biology 2020Quote: ... on a HyD SMD hybrid detector (Leica) with a lifetime gate filter of 1-6 ns to reduce background autofluorescence due to chlorophyll (tobacco ...
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bioRxiv - Neuroscience 2023Quote: ... using the Perfusion Two TM Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL).
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bioRxiv - Biophysics 2020Quote: ... equipped with hybrid photon counting detectors (HyD; Leica). Fluorescence was collected through a 63x 1.40 NA oil immersion objective (HC PL APO 63x/1.40 Oil CS2 ...
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bioRxiv - Cell Biology 2020Quote: ... equipped with hybrid photon counting detectors (HyD; Leica). The sample was excited sequentially frame by frame with 408 nm and 488 nm with the detection set to 500-535 nm ...
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bioRxiv - Cell Biology 2021Quote: ... and captured with a hybrid detector (Leica HyD) controlled using the C software ...
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bioRxiv - Cell Biology 2023Quote: ... using a Leica HyD Hybrid detector (Leica Microsystems) and the Leica Application Suite X (LASX v2.0.1) ...
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bioRxiv - Neuroscience 2022Quote: ... guided the identification and targeting of different brain areas (Angle Two Stereotaxic System, Leica Biosystems). Coordinates for injections and/or implantations in the RFO were 1.5-1.88 mm anterior from Bregma ...
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bioRxiv - Neuroscience 2023Quote: ... the rats were perfused transcardially using the Perfusion Two™ system (Leica Biosystems, Chicago, IL). As recommended by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: All stereotaxic injections were conducted with a computer guided system (Angle Two, Leica Biosystems, USA) as previously described [6] ...
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bioRxiv - Cell Biology 2019Quote: ... 660 nm depletion laser and Hybrid Detector (Leica HyD).
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bioRxiv - Plant Biology 2022Quote: ... A HyD ‘Hybrid’ Super Sensitivity SP Detector (Leica Microsystems) and a PMT SP Detector (Leica Microsystems ...
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bioRxiv - Biophysics 2022Quote: ... a hybrid photomultiplier tube as the detector (HYD-SMD, Leica) and a field-of-view scanner (200 Hz ...
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bioRxiv - Biophysics 2022Quote: ... The emission fluorescence was detected with a Hybrid detector (Leica HyD) in photon counting mode ...
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bioRxiv - Biophysics 2022Quote: ... The emission fluorescence was detected with a Hybrid detector (Leica HyD) in photon counting mode ...
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bioRxiv - Cell Biology 2023Quote: ... Resolution was enhanced by high sensitivity hybrid GaAsP detectors (HyD, Leica), which allowed low intensity illumination for image averaging with minimum bleaching ...
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bioRxiv - Neuroscience 2024Quote: ... Emitted photons were detected with a hybrid detector HyD SMD (Leica). The detector was cooled by a recirculating water chiller (Laird ...
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bioRxiv - Neuroscience 2021Quote: ... Images were acquired in sequential mode with a Hybrid detector (HyD, Leica). Deconvolution with experimental point spread function from fluorescent beads using a maximum likelihood estimation algorithm was performed with Huygens software (Scientific Volume Imaging) ...
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bioRxiv - Biophysics 2021Quote: ... with 1.5-5 ns time gating using a hybrid detector (Leica Microsystems). 512 × 512 pixel images were acquired with a pixel size of 20 nm.
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bioRxiv - Cell Biology 2022Quote: ... The signal was detected using a gated hybrid detector (HyD) (Leica Microsystems) in photon-counting mode.
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bioRxiv - Cell Biology 2022Quote: ... The signal was detected using a gated hybrid detector (HyD) (Leica Microsystems) in photon-counting mode.
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bioRxiv - Neuroscience 2023Quote: ... Emission was collected with a Hybrid Detector (HyD, Leica Microsystem; gain 100). Images were deconvolved using Huygens Professional 22.10 (SVI ...
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bioRxiv - Neuroscience 2019Quote: ... then transcardially perfused with 10% sucrose in phosphate buffered saline (PBS) followed by 4% paraformaldehyde in PBS for approximately 90 s each using a Perfusion Two system (Leica). The brain was then removed and postfixed in 4% paraformaldehyde in PBS overnight at 4°C before being moved to a 30% sucrose solution in PBS for cryoprotection ...
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bioRxiv - Cell Biology 2023Quote: ... with 1.50 ns - 9.50 ns time gating using a hybrid detector (Leica Microsystems). Typical pixel size is 40 nm ...
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bioRxiv - Neuroscience 2021Quote: ... 1%-1.5% for maintenance) and positioned in a computer-assisted stereotactic system with digital coordinate readout and atlas targeting (Leica Angle Two). Body temperature was maintained at 36–37°C with a DC temperature controller ...
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bioRxiv - Molecular Biology 2022Quote: ... A digital mouse brain atlas was linked to the stereotaxic frame to guide the identification and targeting of different brain areas (Angle Two Stereotaxic System, Leica Biosystems). We used the following coordinates for injections and implantations in the SSp-ul ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue gel hybrid was then resliced using a vibratome (VT1200, Leica Biosystems, Germany) in 75 μm (44 μm original ...
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bioRxiv - Physiology 2022Quote: ... In cases where we used a Hybrid detector in the photon counting mode (Leica) we saw no significant departure from our assumption of Poisson distribution ...
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bioRxiv - Biophysics 2023Quote: ... The emitted fluorescence light was collected with Hybrid Detectors (HyD, Leica Microsystems, Mannheim Germany) using appropriate emission band pass-filter setting and typically a gate of 0.3ns-6.0ns for depletion with the 775nm laser or 1.0-6.0ns with the 592nm laser ...
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bioRxiv - Cell Biology 2022Quote: ... a Novolink Polymer Detection Systems Kit (RE7280K; Leica Biosystems Newcastle L10 ...
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bioRxiv - Neuroscience 2022Quote: ... Injections were delivered using pulled glass pipettes (Drummond, 3.5”) in an injection system coupled to a hydraulic micromanipulator (Drummond, Nanoject III) on a stereotaxic frame (Leica, Angle Two™), at approximately 10 nl min−1 ...
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bioRxiv - Neuroscience 2022Quote: ... controlled by Angle Two software (Leica Biosystems). 200nl of AAV5-hSyn-ACh3.0(ACh.4.3 ...
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bioRxiv - Developmental Biology 2023Quote: ... A two-photon confocal laser microscope (Leica) was used to determine vessel density of the whole brain by measuring fluorescence intensity ...
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bioRxiv - Neuroscience 2019Quote: CARS images were acquired using a commercial setup consisting of a picosecond-pulsed laser system that generates two synchronized beams collinearly aligned in an inverted confocal microscope (TCS SP5 II CARS; Leica Microsystems, Heidelberg, Germany). In this setup ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 internal Hybrid (HyD) detectors and Acusto Optical Tunable Filter (Leica microsystems CMS GmbH, Germany) was used and excitation was done by using 488nm by Ar laser and emission was collected using the internal HyD detector at 510-625nm with gain of 100.
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bioRxiv - Physiology 2021Quote: ... CD41-FITC or CD41-PE fluorescence was detected using non-descanned Hybrid detectors (Leica Microsystems) and Hoechst33342 fluorescence was detected using a non-descanned PMT.
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bioRxiv - Microbiology 2020Quote: ... equipped with hybrid detectors and a 63x oil immersion objective (Plan Apo, NA: 1.4, Leica). For each condition ...
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bioRxiv - Microbiology 2021Quote: ... equipped with hybrid detectors and a 63x oil immersion objective (Plan Apo, NA: 1.4, Leica). For each condition ...
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bioRxiv - Physiology 2021Quote: ... and the emitted fluorescence was detected by Leica HyD hybrid detector in the range of 500-700 nm (all from Leica Microsystems, Germany), as described previously (139).
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bioRxiv - Cell Biology 2023Quote: ... equipped with internal Hybrid detectors and acousto-optical tunable filter (Leica Microsystems CMS GmbH, Germany) and a white light laser excitation laser ...
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bioRxiv - Immunology 2022Quote: ... The revelation system (“Bond Polymere Refine” kit, DS9800, Leica) included a secondary antibody HRP (Cell Signaling ...
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bioRxiv - Bioengineering 2021Quote: ... two washes with 95% ethanol (Leica Biosystems, 3803682) for 1 min each ...
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bioRxiv - Bioengineering 2021Quote: ... two washes with 100% ethanol (Leica Biosystems, 3803686) for 1 min each ...
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bioRxiv - Developmental Biology 2021Quote: High-pressure frozen samples were vacuumed to 5 × 10−7 mbar at -120 °C and a fracture was conducted at the interface between the two HPF discs containing the sample (BAF 60, Leica Micro-systems, Vienna, Austria). One disc containing the exposed cryo-fixed ...
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bioRxiv - Neuroscience 2021Quote: ... and sacrificed via transcardiac perfusion with 10% sucrose (isotonic at 9.25% in distilled water; prewash) and 4% paraformaldehyde (PFA; fixative) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Cell Biology 2020Quote: ... equipped with internal Hybrid (HyD) detectors and Acusto Optical Tunable Filter (Leica microsystems CMS GmbH, Germany) and a white light laser (WLL ...
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bioRxiv - Plant Biology 2023Quote: ... EGFP and SR2200 fluorescence emission was collected with HyD ‘Hybrid’ Super Sensitivity SP Detector (Leica Microsystems) and PMT SP Detector (Leica Microsystems) ...