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Citations for Leica :
501 - 550 of 606 citations for Tumor necrosis factor receptor superfamily member 3 LTBR Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Mouse skeletal muscle (quadriceps) was mounted on a piece of cork in Tissue Freezing Medium (12020108926, Leica) with fibers oriented perpendicular to the cork and then snap-frozen in liquid N2-cooled 2-methylbutane and stored at -80°C until sectioning ...
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bioRxiv - Pathology 2021Quote: ... using monoclonal mouse anti-DYS1 (Rod domain, no. NCL-DYS1; clone no. Dy4/6D3, Leica Biosystems, Wetzlar) as primary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse coordinates: AP 1mm-1.4mm from bregma) were sliced on a vibrating microtome (Leica Biosystems, VT1000 S). Serial sections were stored in 0.1M PBS with 0.2% sodium azide until processing ...
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bioRxiv - Neuroscience 2022Quote: Images (n=5 per brain area per mouse) were acquired using a SP8 confocal microscope (LEICA, Germany) with a 63x oil immersion objective (PL APO CS2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse lung sections were imaged at 20X magnification using an Aperio Digital Pathology Slide Scanner (Leica Biosystems).
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bioRxiv - Neuroscience 2024Quote: ... Tissue was then incubated in primary antibody solution made with 1:250 mouse anti-Ki67 (Leica Biosystems), 1% NDS ...
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bioRxiv - Microbiology 2019Quote: Purified RNA was stained with cyanine 3 or cyanine 5 using the ULS Fluorescent Labeling Kit for Agilent arrays from Kreatech (Leica Biosystems, Amsterdam, Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue blocks were then embedded in 3% agarose gel and cut in the sagittal plane at 30 μm-thickness by the vibratome (Leica, Buffalo Grove, IL), counter-stained with Dapi (1:2000 dilution ...
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bioRxiv - Immunology 2019Quote: Pools of 20 larvae were collected at 3- and 4-day post infection (dpi) and imaged by using the Leica MZ16FA Fluorescence Stereo Microscope (Leica Microsystems, Wetzlar Germany) equipped with the DFC420C color camera (Leica Microsystems) ...
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bioRxiv - Cancer Biology 2020Quote: Super-Resolution Stimulated Emission Detection (STED) microscopy (Figure 1A, Figure S1, Figure 4A) was performed using a commercial Leica SP8 STED 3× system (Leica Microsystems, Mannheim, Germany), equipped with a white light laser with continuous spectral output between the wavelengths of 470 nm to 670 nm ...
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bioRxiv - Neuroscience 2020Quote: ... whereas slices processed for immunofluorescence were embedded in 3% agarose solution and re-sliced at 40-µm thickness using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL).
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bioRxiv - Neuroscience 2020Quote: ... the brains were embedded in 3% agar and cut into 40 μm coronal visual cortical sections using a vibratome (Leica Biosystems, Wetzlar, Germany). After washing with PBST (1x PBS and 0.2% TritonX100) ...
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bioRxiv - Plant Biology 2021Quote: ... Cells cultured in M9 or LS medium containing 3% sucrose were added to flat specimen carriers and frozen using a high-pressure freezing machine (Leica EM PACT, Leica Microsystems, Wetzlar, Germany). The frozen samples were transferred to 2% osmium tetroxide in anhydrous acetone at -80 °C and incubated at -80 °C for 6 days (120 h) ...
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bioRxiv - Neuroscience 2020Quote: ... Representative images were taken from the cortical layer 2-3 with Nikon-DS Fi3 camera attached to a Leica DM 2000 Led light microscope (Leica Microsystems GmbH, Germany).
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bioRxiv - Neuroscience 2020Quote: ... Neurons were transduced with LV-Syn1-GFP at DIV 3 and were imaged at DIV 21 using an inverted spinning disk confocal microscope (Leica DMi8, 63x objective). During image recording the cells were maintained in a humidified atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was then embedded in 2 to 3% agar and 100 μm thick sections were cut using a microtome (Leica VT1000 S vibratome). Slices were mounted using a Vectashield HardSet mounting medium containing DAPI (H-1500-10 ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequently the samples were immersed in 100% ethanol for 3×10 min and then critical point dried in an Automated Critical Point Dryer Leica EM CPD300 (Leica Microsystems, Vienna, Austria) with 18 steps of ethanol - CO2 exchange.
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Developmental Biology 2020Quote: Imaging of embryos and tissues was performed using a Leica MZ 16F dissecting microscope equipped with a Leica DFC420 C digital camera supported by the Leica Application Suite software package (LAS, version 3 or 4; Leica Microsystems, Germany/Switzerland), and Leica EL6000 fluorescence light source.
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bioRxiv - Neuroscience 2023Quote: Dual or triple channel STED imaging of Ib and Is AZs was carried out using a Leica SP8 confocal/STED 3× microscope (Leica Microsystems, Mannheim, Germany) with 100X ...
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bioRxiv - Neuroscience 2022Quote: ... We prepared horizontal brain slices containing the somatosensory S2 cortex and the corresponding corticostriatal projection field in the dorsal striatum from mice (2-3-months old) using a vibrating blade microtome (VT1200S, Leica Micosystems, Nussloch, Germany). Brains were sliced in a 5% CO2/95% O2-bubbled ...
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated and subsequently stained with hematoxylin and eosin (H&E) and appropriate antibodies (Supplementary Table 3) using the automated system BOND RX (Leica Biosystems, Newcastle, UK). Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were placed into a petri dish filled with HL-3 on ice and imaged (camera: Leica DFC365 FX, microscope: Leica MZ10 F). Brains were stained against HRP ...
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bioRxiv - Microbiology 2020Quote: ... followed by a polymer-labeled goat anti-mouse IgG coupled with alkaline phosphatase (30 minutes; Powervision, Leica Biosystems). Fast Red was used as the chromogen (15 minutes) ...
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bioRxiv - Developmental Biology 2022Quote: ... adult mouse brain tissue samples were embedded in 4% agarose and sections were cut using vibratome (Leica VT1200S). Until further processing sections were stored in ice-cold 1xPBS ...
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bioRxiv - Neuroscience 2020Quote: 300um thick coronal acute brain slices were prepared from P18-20 mouse using a virbrating microtome (Leica VT1200S) in ice cold cutting solution containing in mM (119 Choline Cl ...
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bioRxiv - Cancer Biology 2021Quote: The following primary antibodies were used: mouse anti-Synaptophysin (1:50, clone 27G12, Leica, NCL-L-SYNAP-299), rabbit anti-Chromogranin A (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... 300μm-thick coronal slices containing the SCN were obtained from PER2::LUC mouse brains using a vibratome (Leica) and placed on a semi-permeable membrane insert (PTFE ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse somatosensory cortical slices (350 μm) were prepared in the coronal plane using a vibratome (Leica 1200 V) in cutting solution ...
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bioRxiv - Pathology 2023Quote: ... Mouse is then placed into a petri dish and onto the stage of an inverted microscope (Leica, DMi8). 4 videos of 90 sec were acquired per mice and the number of contraction and dilatation (difference between maximum and minimum diameter ...
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bioRxiv - Neuroscience 2022Quote: Human and mouse brain tissue was sectioned (30 μm-thick sections) using a SM2010 R sliding microtome (Leica) equipped with a BFS-3MP freezing stage and cooling unit (Physitemp ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were stained manually with mouse anti-TTF1 (clone SPT24; TTF-1-L-CE, Leica, 1/400, 25’) and mouse anti-P40 (clone BC28 ...
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bioRxiv - Neuroscience 2023Quote: ... surface vasculature map was draw on a notebook for each mouse under microscope with light source (Leica M50) and put ‘*’ marker near the location of FOVs in this map for reference when doing the first imaging session ...
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bioRxiv - Neuroscience 2023Quote: ... samples of mouse brain were cut into 100 µm thick sections using a vibratome (Leica VT1200S, Vienna, Austria). Sections containing relevant regions of interest were rinsed in 0.15 M cacodylate buffer containing 2 mM calcium chloride 3 times for 10 minutes each followed by a secondary fixation in 1% osmium tetroxide ...
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bioRxiv - Neuroscience 2023Quote: The 100-µm thickness coronal sections of the fresh mouse brain were prepared by vibrating microtome (Leica VT1200) in in saturated O2 /Glucose+ artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Cell Biology 2020Quote: Sample blocks were obtained by freeze substitution of the cell pellets in 0.5 % uranyl acetate diluted from a 20 % stock in methanol and 3 % double distilled H2O in acetone in a Leica AFS1 (Leica Microsystems GmbH, Wetzlar, Germany). Temperature was set to −130 °C for the first 2 hours and then raised to −90 °C with a 20 °C/hr gradient ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were imaged in brightfield using a Point Grey Flea®3 color camera (Teledyne FLIR, Wilsonville, OR) mounted on a Leica S8 APO stereo microscope (Leica Camera, Wetzlar, Germany). Representative images are shown.
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bioRxiv - Pathology 2021Quote: Four micron thick sections of left and right ventricular FFPE tissue blocks per decedent were cut and labeled using an automated staining platform (Leica Bond 3, Leica Biosystems, Buffalo Grove, IL) with the following monoclonal antibodies against ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were rinsed 3 times in phosphate buffered saline and 50 μM coronal brain slices were obtained using a vibratome (Leica VT1000, Buffalo Grove, Il), without embedding the tissue ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035; Leica Instruments GmbH, Nuβloch, Germany). Sections were stained by hematoxylin and eosin (HE ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC, Leica Microsystem GMbH, Wetzlar, Germany). When the degree of confluence reaches approximately 80 % (every 4-6 days) ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed an additional 3 times in 100% ethanol for 15 minutes each and dried using a Leica EM CPD030 critical point dryer (Leica Microsystems, Buffalo Grove, IL) with CO2 as the transitional medium ...
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bioRxiv - Bioengineering 2023Quote: ... were acquired over a ∼30 mm x ∼3 mm rectangular area by using the tile scan feature of the LAS X software (Leica Microsystems, Buffalo Grove, IL). This involved frame by frame acquisition (36 × 5 = 180 frames ...
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Physiology 2021Quote: ... The lung tissue sections of 3-5 micron were stained with H&E stain and were microscopically analyzed using microscope (Make: Leica DM1000 LED; Camera: Leica MC170HD). The images were taken with 10X objective lens (Magnification X 100 ...
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bioRxiv - Developmental Biology 2022Quote: Images from mouse and human samples were acquired with a Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems). All images are shown as maximum intensity projections of acquired z-stacks of about one cell thickness (6-10 µm) ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 μm transverse zebrafish and mouse cryosections were collected on a Leica CM1900 crysostat (Leica Biosystems, Buffalo Grove, IL). 10 μm paraffin mouse sections were collected on a Thermo Scientific Shandon Finesse ME Microtome (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... transverse slices of 150µm from the hippocampi of a P17 mouse were prepared using a vibrating blade microtome (Leica) in cold (4°C ...
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bioRxiv - Developmental Biology 2020Quote: En face STED images of mouse cephalic explants were taken with a Leica SP8 TCS STED microscope (Leica Microsystems) equipped with a pulsed white-light excitation laser (WLL ...
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bioRxiv - Cancer Biology 2021Quote: ... A polymer detection system that includes an anti-mouse secondary antibody (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied in the tumor samples ...