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Citations for Leica :
251 - 300 of 337 citations for SpectraDye Antibody Labeling Kit IR700 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Expression of MLC1 on the sections was detected using the Bond Polymer Refine Detection Kit (Leica) with specific antibodies and an immunostaining system (Bond III ...
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bioRxiv - Neuroscience 2022Quote: ... Color development was performed with classical DAB (3,3’-diaminobenzidine) staining (Bond Polymer Refine Detection Kit, Leica Biosystems.
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bioRxiv - Microbiology 2023Quote: ... Detection with DAB chromogen was completed using the Bond Polymer Refine Detection kit (Leica Biosystems, #DS9800). Slides were finally cleared through gradient alcohol and xylene washes prior to mounting and placing cover slips ...
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bioRxiv - Immunology 2023Quote: ... platform according to manufacturer-supplied protocols using the Bond Polymer Refine Red Detection kit (Leica Biosystems) along with the RNAscope® 2.5 LS Reagent kit-Red (Advanced Cell Diagnostics ...
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bioRxiv - Cell Biology 2022Quote: ... with a Bond RX auto-stainer using the Bond Polymer Refine Kit (Leica Biosystems, Cat# DS9800) minus the peroxidase block ...
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bioRxiv - Physiology 2024Quote: ... the Leica Bond max robot was used with the BOND Polymer Refine Detection kit (Leica, DS9800). Primary antibodies were anti-Iba-1 (Wako ...
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bioRxiv - Immunology 2021Quote: ... primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded tissues was carried out on a Discovery XT automated slide stainer using a mouse anti-human monoclonal antibody for FOLR1 (Novocastra Clone BN3.2; Leica Biosystems, Wetzlar, Germany) at 15 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Cancer Biology 2022Quote: ... was applied on tissue sections for 25 minutes incubation and the antigen-antibody binding was detected with Bond polymer refine detection (Leica Biosystems, DS9800). The tissue sections were covered with cover glasses ...
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bioRxiv - Physiology 2022Quote: ... Slides were washed and incubated with anti-rabbit poly-HRP-IgG secondary antibody (Novolink Polymer Detection System, Leica Biosystems, cat# RE7200-CE) for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunofluorescence was performed using Leica Bond RX stainer and the Bond Polymer Refine Detection kit (Leica, Germany). Antibodies and detection ...
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bioRxiv - Cancer Biology 2020Quote: ... The expression of proteins was detected using the Bond Polymer Refine Detection kit (Leica Biosystems, Cat# DS9800) with diaminobenzidine as chromogen (88) ...
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bioRxiv - Cancer Biology 2020Quote: ... a standardized automated protocol using the Leica Bond Polymer Refining Kit (Leica Bond-III stainer, Leica Biosystems) was performed ...
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bioRxiv - Cancer Biology 2020Quote: ... a standardized automated protocol using the Leica Bond Polymer Refining Kit (Leica Bond-III stainer, Leica Biosystems) was performed ...
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bioRxiv - Physiology 2021Quote: ... DAB chromogen and hematoxylin counterstaining was performed automatically using Bond polymer refine detection kit (Leica Biosystems, DS9800). The degree of interstitial cell infiltration was determined using immunostaining targeting macrophages (CD68) ...
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bioRxiv - Immunology 2023Quote: ... Detection with DAB chromogen was completed using the Bond Polymer Refine Detection kit (Leica Biosystems, Catalog # DS9800). Slides were finally cleared through graded alcohol and xylene washes prior to mounting and placing cover slips.
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared at a thickness of 70 μm for imaging intrinsic fluorescence or 40 µm for antibody staining (Leica VT 1000S vibratome). For immunohistochemistry ...
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bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Neuroscience 2023Quote: ... Colocalization of the antibody immunofluorescence signal and endogenous fluorescence of NG2 glia was examined using the Leica Stellaris confocal platform (Leica Microsystems, Wetzlar, Germany). The immunopositive areas of the cortex surrounding the ischemic injury were further quantified using Fiji ImageJ software (NIH ...
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bioRxiv - Immunology 2023Quote: ... Formalin-fixed paraffin-embedded (FFPE) sections of 4 μm thickness were stained with antibodies specific for CD21 (predilute, clone 2G9, Leica Biosystems, Danvers, MA), CD3 (predilute ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Glial fibrillary acid protein (GFAP) Iimmunohistochemistry was carried out using GFAP primary monoclonal antibody and the Novocastra™ and Novolink DM polymer detection system (Leica Biosystems, UK) as described previously (39–42).
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bioRxiv - Neuroscience 2023Quote: Neuron specific enolase (NSE) immunohistochemistry was carried out using NSE primary monoclonal antibody and the Novocastra™ and Novolink DM polymer detection system (Leica Biosystems, UK) as described previously [54 ...
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated and subsequently stained with hematoxylin and eosin (H&E) and appropriate antibodies (Supplementary Table 3) using the automated system BOND RX (Leica Biosystems, Newcastle, UK). Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: IHC was performed using the Power Vision+ Poly-HRP IHC kit (Cat. No. PV6119, Leica Biosystems, Wetzlar, Germany). Slides were steamed for 25 minutes in antigen retrieval solution (Cat ...
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bioRxiv - Immunology 2022Quote: ... CA) was used with the Leica RNAScope 2.5 LS Assay-RED kit and a hematoxylin counterstain (Leica Biosystems). Slides were treated in protease (Advanced Cell Diagnostics ...
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bioRxiv - Genomics 2020Quote: ... All brightfield immunohistochemistry was completed using individually optimized protocols using Research Detection Kits on a BOND RX (Leica) system ...
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bioRxiv - Neuroscience 2020Quote: ... Immunostaining was performed using reagents provided by the Bond Polymer Refine Detection kit (DS9800, Leica Biosystems, Wetzler, Germany). Sections were blocked with Peroxide Block solution (Leica Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... The sections were incubated with CD8 mouse monoclonal antibody (clone 4B11, 1:150, 0.2 µg IgG2b/ml) from Novocastra (Leica Microsystems, Newcastle Upon Tyne, UK) for 45 minutes ...
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bioRxiv - Cancer Biology 2020Quote: Five micron sections of tumors were immunolabeled with one of the following antibodies using a Leica Bond III automated immunohistochemistry instrument (Leica Biosystems, Buffalo Grove, IL): Rabbit anti-CD3 ...
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bioRxiv - Cancer Biology 2022Quote: Tissue sections were incubated for 1 hour in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... using Leica Bond III automated staining system with diaminobenzidine chromogen detection and the same antibodies as for clinical diagnostics (DS9800, Leica Biosystems, Buffalo Grove, IL). Hematoxylin and eosin stain was used for general histopathology ...
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bioRxiv - Cancer Biology 2023Quote: Tissues sections were incubated for 1 h in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: to detect muscle fibre types we used mouse anti- myosin heavy chain (slow) (Leica, clone WB-MHCs; 1:200); to delimit the fibres we used rabbit anti-collagen type VI (Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... dehydrated in an ethanol series and embedded in LR White resin (Historesin™ Embedding Kit, Leica Instruments, Heidelberg, Germany) prior to sectioning ...
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bioRxiv - Cancer Biology 2021Quote: ... staining were performed using the Leica BOND-III automated staining system and the Leica refine detection kit as per manufacturer’s instructions (Leica). Trypsin-based antigen retrieval was used for F4/80 staining ...
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bioRxiv - Cancer Biology 2021Quote: ... Staining was performed on the BOND-RX automated staining platform using Bond Polymer Refine Detection automated kit (Leica Biosystems). H&E staining was performed manually using a standard protocol.
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bioRxiv - Cancer Biology 2022Quote: ... peroxidase blocking was carried out for 10 min using the Bond Polymer Refine Detection Kit DS9800 (Leica Microsystems GmbH). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... Advanced Cell Diagnostics) was performed using RNAscope 2.5 LS Reagent Kit–BROWN (Advanced Cell Diagnostics) on a BOND RX autostainer (Leica) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... IHC staining for UGDH was performed using Novolink Polymer Detection Systems kit (RE7150-CE, Leica Microsystems, Mt Waverley, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Detection of the probes was performed through the BOND polymer Refine Red Detection and haematoxylin kit (DS9390, Leica Bond). Haematoxylin (blue ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were stained with the Bond Polymer Refine Red Detection kit using a Bond Rxm automated stainer (Leica Biosystems). Following a 20 min antigen retrieval in pH 6 citrate buffer (CLPP ...
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bioRxiv - Cancer Biology 2021Quote: ... from A549 tumors were used for hematoxylin and eosin (H&E) staining and proliferation analysis with anti-Ki67 antibody (Leica Biosystems; Cat#: NCL-Ki-67p). Briefly ...
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bioRxiv - Cell Biology 2019Quote: Quantification of protein by Western blotting was carried out using standard techniques and the following primary and secondary antibodies: anti-α-SMA (SMA-R-7-CE, Leica Biosystems, dilution: 1:100), anti-COL1 (1310-01 ...
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bioRxiv - Pathology 2023Quote: ... Diluted primary antibody (CST #8242 1:800; CST #99940, 1:150; CST #9718, 1:500; Abcam ab107099, 1:X; Leica P53-CM5P-L 1:200) was applied and slides were incubated at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... Single-molecule fluorescent mRNA in situ hybridization was conducted using RNAscope (Advanced Cell Diagnostics) probes and kits optimized for use on a BOND RX (Leica) system ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were stained using a Leica BOND-MAX-TM autostainer with BondTM epitope retrieval 1 or 2 and the Bond Refine Detection Kit (Leica). Primary antibodies are listed in Supplementary Table S1.
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bioRxiv - Cancer Biology 2020Quote: ... IHC technique was performed with commercial solutions from the Novolink™ Max Polymer Detection System Kit (Leica Biosystems, Newcastle UK). Before antibody incubation ...
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bioRxiv - Cell Biology 2021Quote: ... IHC staining was performed using the Novocastra Novolink™ Polymer Detection Systems kit (Code: RE7280-K, Leica, Biosystems, Newcastle, UK) as previously described 79 ...
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bioRxiv - Pathology 2021Quote: ... CA) was used with the Leica RNAScope 2.5 LS Assay-RED kit and a hematoxylin counterstain (Leica Biosystems, Richmond, IL). Slides were treated in protease (Advanced Cell Diagnostics ...