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Citations for Leica :
1 - 50 of 557 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and transferred for cryo-SEM imaging using a vacuum cryo-transfer device (VCT 100, Leica Micro-systems, Vienna, Austria). Finally ...
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bioRxiv - Bioengineering 2019Quote: ... The surface morphology of the samples was assessed by cryo-SEM (SEM LEO-1530, Zeiss, Oberkochen with cryo stage Leica, Vienna) at −120 °C after sample freeze fracture or after freeze fracture and freeze-drying performed as described in Table S4.
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bioRxiv - Developmental Biology 2021Quote: Vitrified fractured primary polyp specimen were transferred from the SEM under a cryogenic temperature via VCT (VCT 100; Leica Microsystems) to the freeze-fracture device (BAF 060 ...
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bioRxiv - Developmental Biology 2021Quote: ... freeze-fractured primary polyp was transferred for cryo-SEM imaging using a vacuum cryo-transfer device (VCT 100, Leica Micro-systems, Vienna, Austria) and loaded into the SEM where it was imaged at -120 °C.
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bioRxiv - Microbiology 2022Quote: ... and mounted into a 40 ° pre-tilted SEM grid holder (Leica Microsystems). The holder was transferred with a VCT100 cryo-transfer system (Leica Microsystems ...
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bioRxiv - Plant Biology 2023Quote: ... then mounted on SEM stubs and sputter coated in the ACE600 coater (Leica). SEM image maps were acquired using a ThermoFisher ApreoVS SEM and MAPS version 3.9 software (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: The waffle grid was placed into a 40° pre-tilted cryo-FIB/SEM grid holder (Leica Microsystems) and inserted into the FEI Helios NanoLab 650 FIB/SEM (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... ultrathin sections were produced from the samples for SBF-SEM observation with an ultramicrotome (Ultracut UCT, LEICA). These sections were mounted on copper grids and observed with HT7700 (Hitachi High-Tech Co.).
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bioRxiv - Neuroscience 2019Quote: ... the sample was removed from the SEM chamber and cut using a physical ultramicrotome (Ultracut EM UC7; Leica Microsystems, Germany). The resulting 30-50nm-thick slices obtained were sequentially collected on EM grids (EMS Diasum ...
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bioRxiv - Molecular Biology 2021Quote: ... The surface of the cocoon and all the fibers were observed using scanning electron microscopy (SEM, Hitachi, Japan) at an acceleration voltage of 5 kV and a polarizing optical microscope (Leica, Germany). For transmission electron microscope (TEM ...
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bioRxiv - Neuroscience 2021Quote: ... The small epon block was mounted on a regular SEM stubs using conductive carbon and coated with 25 nm of platinum (using High Vacuum Coater, Leica ACE600).
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bioRxiv - Developmental Biology 2019Quote: ... mounted on fixed 45° pretilt SEM stubs and coated with a 2 nm layer of platinum using a Leica Microsystems EM ACE600 sputter coater (Leica Microsystems) to enhance electrical conductivity ...
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bioRxiv - Pathology 2023Quote: Carriers containing high pressure frozen samples for cryoFIB-SEM liftout were transferred to a cryo-ultramicrotome (Leica EM FC7, −160°C) equipped with a Trim 45 T1865 diamond knife ...
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bioRxiv - Neuroscience 2021Quote: ... snap frozen in a dry-ice chilled methanol bath and sectioned in a cryostat (Leica) at 18-μm thickness (unless otherwise specified) ...
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bioRxiv - Cell Biology 2023Quote: ... The resin blocks were mounted on an SEM stub using a conductive epoxy resin glue and with silver resin paste and then sputter-coated with carbon (Leica EM ACE600) to enhace sample conductivity before being loaded onto a multi-purpose holder in the SEM sample chamber ...
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bioRxiv - Biochemistry 2023Quote: ... the grid was blotted for 1.5 s on the other side under 99% humidity and at 20 °C using EM GP2 plunge freezer (Leica) and immediately plunged into liquid ethane ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then transferred to 99% glycerol for imaging on a binocular microscope using Leica Application Suite (LAS 3.3, Leica).
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bioRxiv - Cell Biology 2021Quote: ... samples were mounted on aluminum SEM stubs and sputter-coated with a 4 nm platinum-palladium layer using a Leica EM ACE600 sputter coater (Leica Microsystems, Illinois, USA). Images were acquired at 2 kV using a Zeiss Sigma 300 SEM (Zeiss ...
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bioRxiv - Cell Biology 2021Quote: ... resin blocks were mounted onto SEM pins with silver epoxy and sputter coated with 6 nm of iridium (Leica ACE 600, Vienna, Austria) at a 45° angle with rotation on a planetary stage to ensure the entire block was coated.
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bioRxiv - Neuroscience 2020Quote: ... Samples were transferred into cold methanol (−90°C) in a freeze-substitution chamber (Leica EM AFS). Methanol was replaced three times before the specimens were immersed overnight in anhydrous methanol at −90°C ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were dehydrated in methanol at -80°C and embedded by freeze-substitution (Leica EM AFS2) in Lowicryl HM 20 (Electron Microscopy Science ...
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bioRxiv - Microbiology 2020Quote: ... 2.3% methanol and 1–3% H2O in Acetone) tempered at −90°C in FS device (Leica AFS2) equipped with a robotic solution handler (Leica FSP) ...
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bioRxiv - Cell Biology 2020Quote: ... in dry methanol for 32 hours at −90° C in an AFS 2 freeze substitution unit (Leica Biosystems). The temperature was then raised 5° C per hour to −45° C ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were equilibrated in 30% methanol and deposited in a Leica EM AFS2/FSP automatic reagent handling apparatus (Leica Microsystems). Lowicryl polymerization under UV was for 40 h at – 20°C followed by 40 h at +20°C Ultrathin-sections of Lowicryl-embedded material were collected on Formvar-carbon-coated gold grids (200 mesh ...
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bioRxiv - Systems Biology 2022Quote: ... followed by a blocking step for endogenous peroxidase using 0.3% H2O2/methanol and fixation with 10% neutral buffered formalin (Leica Biosystems, Germany). Slides were washed in Milli-Q water and 0.05% Tween20 in 1x Tris-Buffered Saline (TBS-T) ...
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bioRxiv - Cell Biology 2023Quote: ... cell pellets were equilibrated in 30% methanol and deposited in a Leica EM AFS2/FSP automatic reagent handling apparatus (Leica Microsystems). Lowicryl polymerization under UV was performed for 48 h at – 20°C ...
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bioRxiv - Cell Biology 2020Quote: Sample blocks were obtained by freeze substitution of the cell pellets in 0.5 % uranyl acetate diluted from a 20 % stock in methanol and 3 % double distilled H2O in acetone in a Leica AFS1 (Leica Microsystems GmbH, Wetzlar, Germany). Temperature was set to −130 °C for the first 2 hours and then raised to −90 °C with a 20 °C/hr gradient ...
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bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
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bioRxiv - Neuroscience 2023Quote: ... the mice were perfused intracardially with heparinized 0.1 M PBS (10 U/ml of Heparin, Ratiopharm, N68542.03; 100-125 mmHg pressure using a Leica Perfusion One system) for 5 minutes to remove the blood ...
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bioRxiv - Bioengineering 2021Quote: ... they were intracardially perfused with cold heparinized 0.1 M PBS (10 U/mL of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Cancer Biology 2019Quote: ... before intracardial perfusion with heparinized 0.01 M PBS (10 U/ml of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Cell Biology 2022Quote: ... 100 × 1.45 NA objective (Leica), and an Evolve 512Delta EMCCD (Teledyne Photometrics ...
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bioRxiv - Cell Biology 2020Quote: ... 100×1.45 NA objective (Leica), and an Evolve 512Delta EMCCD (Photometrics ...
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bioRxiv - Plant Biology 2023Quote: ... 100% ethanol) and incubated overnight in a 1:1 solution of 100% ethanol:Historesin solution (Leica). The solution was exchanged with Historesin and the samples incubated again overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... using an HCX PL APO 100×100/1.40/0.70 oil immersion objective lens (Leica Microsystems, Mannheim, Germany). Emission depletion was performed with 592 nm CW STED laser ...
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bioRxiv - Developmental Biology 2019Quote: ... β-Dystroglycan (1:100; Leica #NCL-b-DG), aPKCς clone C-20 (1:400 ...
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bioRxiv - Neuroscience 2022Quote: ... High-pressure freezing instrument (HPM 100, Leica) was used to preserve membrane structure ...
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bioRxiv - Bioengineering 2021Quote: ... two washes with 100% ethanol (Leica Biosystems, 3803686) for 1 min each ...
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bioRxiv - Cancer Biology 2020Quote: ... was diluted 1:100 in Antibody Diluent (Leica).
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bioRxiv - Cell Biology 2020Quote: ... Images were acquired at 100 times magnification (Leica), and the number of cells was counted with the software ImageJ ...
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bioRxiv - Molecular Biology 2023Quote: ... A ×100 oil-immersion objective (Leica, N.A. 1.4) was employed.
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bioRxiv - Neuroscience 2024Quote: ... 100-μm-thick free-floating vibratome (Leica VT1200) coronal sections were cut at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... samples were dehydrated in a graded ethanol series (50,70, 90, 100 and 100%) for 10 minutes each and then critical point dried (Leica CPD 300). Glass slips were adhered to a conductive carbon adhesive tab on aluminum stubs ...
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bioRxiv - Cell Biology 2021Quote: ... Osmicated samples were dehydrated in graded ethanols (25% to 100%) at 4°C and stored in 100% acetone until critical point drying with liquid CO₂ (EM CPD300, Leica Microsystems Ltd.). Samples were mounted onto stubs using silver paint (Agar Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... 100 μM thick coronal sections were cut (Leica, VT1200s). Sections containing LC were incubated with rabbit anti-Tyrosine Hydroxylase (TH ...
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bioRxiv - Cell Biology 2022Quote: ... sectioned with a vibratome (100 mm thickness; VT1200s; Leica) and immune stained ...
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bioRxiv - Neuroscience 2022Quote: ... using liquid nitrogen under high pressure (Leica HPM 100). Samples then underwent freeze substitution (Reichert AFS ...
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bioRxiv - Biochemistry 2023Quote: ... using a VCT 100 Vacuum Cryo Transfer device (Leica). After fracturing at a temperature of −120°C ...
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bioRxiv - Neuroscience 2023Quote: ... using liquid nitrogen under high pressure (Leica HPM 100). After freezing ...
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bioRxiv - Cell Biology 2023Quote: ... anti-α-SMA (NCL-L-SMA, Leica, 1:100), anti-SOX9 (ab5535 ...