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Citations for Leica :
51 - 100 of 109 citations for Recombinant Rabbit IL6 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and visualization systems (Bond Polymer Refine Detection, Bond, Leica; Omni Map anti-(rabbit,mouse,rat) Ventana ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with HRP-conjugated anti-rabbit secondary antibody (Leica Microsystems, cat. PB6119) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... To compare the localization of the two proteins we used the SP8 STED microscope (Leica, with a 100× oil immersion objective and the LAS X Leica software) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein localization was visualized by a confocal laser scanning microscope (Leica TCS SP8; Leica Microsystems) with a 40X/1.30 oil immersion objective ...
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bioRxiv - Biochemistry 2022Quote: ... Protein localization was visualized by a confocal laser scanning microscope (Leica TCS SP8; Leica Microsystems) with a 40X/1.30 oil immersion objective ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibodies were PowerVision Poly-HRP anti-rabbit or anti-mouse (Leica, PV6114 and PV6119) and developed with DAB.
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bioRxiv - Plant Biology 2020Quote: Cells expressing GFP-tagged proteins were examined using a Leica confocal microscope SP8X (Leica Microsystem, Germany) with 40×/1.0 water-dipping objective ...
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bioRxiv - Cell Biology 2022Quote: ... Protein location of GATA1 and p50 were detected by immune-florescence using Confocal Microscopy (Leica DMI6000). Nuclear protein was isolated from baseline Zfp467+/+ and -/- BMSCs lysate by Pierce™ NE-PER® Nuclear and Cytoplasmic Extraction Reagent Kit (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... Western blot was performed according to the supplier’s instructions using rabbit polyclonal anti-p53 antibody (CM5, Leica) and rabbit-monoclonal anti-vinculin antibody (EPR8185 ...
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bioRxiv - Genetics 2021Quote: ... Protein localization was visualized by fluorescence confocal microscopy on a Leica SP5 microscope (Leica Microsystems, Wetzlar, Germany) and images were captured with LASAF as described previously [20].
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bioRxiv - Cancer Biology 2020Quote: ... Protein expression was quantified as positive pixels using Aperio Positive Pixel Count Algorithm software (Leica Biosystem, IL).
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bioRxiv - Cancer Biology 2020Quote: ... The expression of proteins was detected using the Bond Polymer Refine Detection kit (Leica Biosystems, Cat# DS9800) with diaminobenzidine as chromogen (88) ...
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bioRxiv - Neuroscience 2022Quote: ... The images of the targeting proteins were analyzed by high solution confocal microscopy (LEICA TCS SP8, Germany). Control results using only the 2nd antibodies were shown in supplementary Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein localization was analyzed 24-48 hours after infiltration by confocal laser scanning microscope (TCS SP8, Leica). GFP was excited at 488 nm and the fluorescence detected between 498-525 nm.
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bioRxiv - Cell Biology 2020Quote: Prior to staining for collagen type II the sections were blocked with Novolink protein (Leica Biosystems, Weltzlar, Germany) for 5 minutes at room temperature ...
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An annotation dataset facilitates automatic annotation of whole-brain activity imaging of C. elegansbioRxiv - Neuroscience 2019Quote: ... The fluorescence of the fluorescence proteins and the dye was observed sequentially using laser scanning confocal microscopy (Leica SP5 with 63× water immersion lens and 2× zoom) ...
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bioRxiv - Biochemistry 2021Quote: ... proteins fixed in 30% ethanol/15% acetic acid and the gel piece embedded in tissue embedding media (Leica). After careful mounting on a cryo-holder ...
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bioRxiv - Cell Biology 2024Quote: ... Cells that expressed the fluorescent fusion protein were identified using an epifluorescence microscopy (DM6000B, Leica Microsystems, Wetzlar, Germany) equipped with an oil immersion objective (1.4 NA ...
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bioRxiv - Immunology 2020Quote: ... and rabbit anti-rat secondary (Vector) DAPI was used to stain nuclei in Bond Polymer Refine Detection (Leica Biosystems) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with phospho-DRP1 (Ser 616 ...
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bioRxiv - Plant Biology 2021Quote: ... plant tissues expressing GFP-or RFP-fused proteins were imaged with a Leica TCS SP8 confocal microscope (Leica Microsystems) using the preset settings for GFP (Ex ...
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bioRxiv - Cell Biology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with the primary antibodies.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were mounted once every 80 μm and mCherry fluorescent protein expression was detected under a microscope (Leica DMI6000). Microphotographs were taken at 2.5X magnification of each section where mCherry fluorescent signal was seen.
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bioRxiv - Cancer Biology 2021Quote: ... and the following primary and secondary antibodies were used: rabbit anti-p53 (Leica Biosystems, NCL-L-p53-CM5p, 1:5000), rabbit anti-Mettl3 (Abclonal ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed with TBST and incubated with PowerVision Poly-HRP anti-rabbit IgG or anti-mouse IgG (Leica Biosystems) in a humidified chamber at 37°C for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... for 8 minutes at room temperature (Poly-HRP-mouse anti-rabbit IgG, BOND Polymer Refine Detection System, Leica, Germany; DS9800). A color reaction with 3,3-di-amino-benzidine (DAB chromogen ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with a secondary antibody (rabbit anti-mouse IgG, BOND Polymer Refine Detection System, Leica, Germany, DS9800) for 8 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Images of immunostained cells or cells expressing fluorescent tagged proteins were acquired on Confocal inverted microscope SP8-X (DMI 6000 Leica). Optical sections were acquired with a Plan Apo 63x oil immersion objective (N.A ...
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bioRxiv - Biochemistry 2020Quote: ... Protein mixture (5 μL) were deposited onto the grids and blotted for 3 seconds using an Automatic Plunge Freezer (Leica).
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bioRxiv - Bioengineering 2020Quote: ... coli constitutively expressing the Green Fluorescent protein present on biofilms was examined by laser scanning confocal microscopy (Leica TCS SP8) using oil immersion at 40X magnification ...
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bioRxiv - Plant Biology 2023Quote: ... Each 3 µl of protein reaction mix was loaded into a flow-chamber and immediately observed with a confocal microscope SP5 (Leica) equipped with a 60x oil objective (NA 1.42) ...
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bioRxiv - Plant Biology 2024Quote: ... Strains exhibiting mNeonGreen fluorescence were used to localize the RMT2-mNeonGreen fusion protein by TCS SP8 confocal laser-scanning microscopy (Leica); excitation/emission settings were 488 nm/515-530 nm using the HyD SMD hybrid detector for mNeonGreen ...
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bioRxiv - Plant Biology 2024Quote: ... the eYFP fluorescence of fusion proteins was assayed 2–3 days after infiltration using a confocal microscope (Leica TCS SP8). For the Split–LUC assay ...
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bioRxiv - Microbiology 2023Quote: ... tissue sections were incubated with the primary antibody (rabbit polyclonal anti-Influenza A virus NP [ThermoFisher Scientific, PA5-32242] diluted 1:500 in Primary Antibody diluent [Leica Biosystems]) for 30 min at ambient temperature followed by a polymer-labeled goat anti-rabbit IgG coupled with alkaline phosphatase (30 min) ...
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bioRxiv - Biochemistry 2023Quote: ... Rabbit mAb (Mouse Preferred; IHC Formulated) (CST, #12202)) was used 1:200 and developed with Bond Polymer Refine Detection (Leica, DS9800).
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bioRxiv - Cancer Biology 2023Quote: ... Goat anti-rabbit-HRP was used as the secondary antibody with the BOND polymer refine detection system (Leica, catalog no. DS9800). Images were taken by Zeiss LSM710 confocal microscope with a 10X ...
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bioRxiv - Plant Biology 2021Quote: ... the expression of TiAP1 and TaSYP51 proteins were observed and analysed using a confocal laser-scanning microscope (Leica SP5 - X, Germany) according to the methodology of Smigielski et al ...
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bioRxiv - Cancer Biology 2020Quote: ... and after two washing steps with PBS the nonspecific binding of immunoglobulins was prevented by incubating the tissue slices with the Protein Block Novocastra Solution (Leica Biosystems) for 60 min ...
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bioRxiv - Molecular Biology 2020Quote: Cells expressing EGFP protein were visualized 48 h post-transfection by fluorescent microscopy using Leica DMi8 inverted microscope (Leica Microsystems, Germany) at magnification 10x ...
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bioRxiv - Cell Biology 2022Quote: ... Each protein mixture (14 μM for each component) was injected into a homemade chamber and imaged using a Leica microscope (Leica DMi8) using a 40X objective lens ...
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bioRxiv - Cell Biology 2020Quote: ... To compare the localization of the two proteins we used the SP8 STED microscope (Leica, with a 100× oil immersion objective and the LAS X Leica software). Images were acquired as z-stacks with a z-step size of 120 nm and a X-Y resolution of 37.9 nm ...
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bioRxiv - Plant Biology 2022Quote: ... the protein fluorescence was confirmed with the Leica TCS LSI macroscope with Plan APO 5x and 20x objectives (Leica Microsystems, Germany), using the settings described previously83 ...
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bioRxiv - Cell Biology 2023Quote: ... as well as live tissues expressing fluorescently tagged proteins were imaged confocally using a Falcon SP8 laser scanning system (Leica Microsystems) with a 1.2-numerical aperture ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Plant Biology 2024Quote: ... The presence of Cas9 in the shoot apical meristem was investigated by observing the fluorescence of the Cas9-GFP protein (Venus-Cas9) using a confocal laser scanning microscope (TCS SP5, Leica Microsystems). The area of each cell within the SAM was calculated using SEM images of the SAM with Image J software (https://imagej.nih.gov/ij/download.html).
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Physiology 2022Quote: ... Slides were washed and incubated with anti-rabbit poly-HRP-IgG secondary antibody (Novolink Polymer Detection System, Leica Biosystems, cat# RE7200-CE) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Figures from double immunofluorescence labeling of GLP-1R and GFAP proteins were obtained on a Confocal Leica SP5 microscope (Leica Microsystems, France).