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Citations for Leica :
51 - 100 of 124 citations for Recombinant Human S100 Calcium Binding Protein A1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Platelet interactions with human monocytes were imaged in a Leica TCS SP5 SMD confocal system (Leica Microsystem). Cells were seeded in microslide 8-Well IBIDI chamber ...
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bioRxiv - Microbiology 2021Quote: ... as follows: SARS-CoV-2 Spike protein: 1:1000 (Leica, AR9961) 30minutes ...
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bioRxiv - Cell Biology 2023Quote: ... slides were incubated with antibodies to p53 protein (CM5) (Leica Biosystems), EpCAM (CD326 ...
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bioRxiv - Immunology 2023Quote: ... and blocked for 30 min with Protein Block (Leica Novocastra, UK). Primary antibodies were added without washing the blocking solution and incubated overnight at 4 0C ...
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bioRxiv - Neuroscience 2022Quote: Human and mouse brain tissue was sectioned (30 μm-thick sections) using a SM2010 R sliding microtome (Leica) equipped with a BFS-3MP freezing stage and cooling unit (Physitemp ...
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bioRxiv - Plant Biology 2020Quote: ... Fluorescent protein reporters were imaged using a spinning-disk confocal microscope (Leica DMi8 inverted microscope ...
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bioRxiv - Cell Biology 2020Quote: ... Prior to staining the sections were blocked with Novolink protein (Leica Biosystems) for 5 minutes at room temperature ...
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bioRxiv - Genetics 2021Quote: ... Slides were incubated with primary antibody against p53 protein (CM5; Leica Biosystems) and then with secondary antibody ...
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bioRxiv - Immunology 2022Quote: ... protein block was applied for 30 mins (Leica Microsystems, catalog no. RE7102) and stained with KDM6B (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... the slides were blocked with Protein Blocker (Novocastra Peroxidase Detection System, Leica) and 5 % skim milk ...
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bioRxiv - Cell Biology 2023Quote: ... Deconvolution and kinetochore protein quantification were performed with LAS X software (Leica).
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bioRxiv - Developmental Biology 2022Quote: Images from mouse and human samples were acquired with a Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems). All images are shown as maximum intensity projections of acquired z-stacks of about one cell thickness (6-10 µm) ...
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bioRxiv - Immunology 2022Quote: ... serial human kidney sections were stained with anti-CD3 (LN10) or anti-CD8 (4B11; both Leica Biosystems, Chicago, IL) antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... automated methods on the Leica Bond Max autostainer using a mouse monoclonal anti-human CD3 antibody (Leica, clone LN10). Whole slide images of IHC stained slides were digitally captured using an Olympus IX83 microscope at 10x magnification and fixed exposure times ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins and complexes were visualised by confocal microscopy (Zeiss LSM700 and Leica SP5).
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bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
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bioRxiv - Cell Biology 2021Quote: Fixed and stained human pancreatic tissue sections were examined using a commercially available DragonFly200 spinning disk system (Andor) on a DMi8 microscope (Leica) with Plan Apo 40× NA 1.3 and Plan Apo 63× NA 1.4 oil immersion objectives ...
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bioRxiv - Cancer Biology 2021Quote: All staining of human tissues was performed on a Leica Bond RX automated stainer (Leica Microsystems, Inc., Buffalo Grove, IL). To ensure lectin specificity ...
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bioRxiv - Bioengineering 2022Quote: ... Bio SB) or anti-human CD163 (clone EP324, Bio SB) followed by an HRP-conjugated anti-mouse secondary antibody (Leica) and revealed with the peroxidase substrate 3 ...
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bioRxiv - Neuroscience 2023Quote: ... The entire human DRG section was imaged and merged using Leica Application Suite X (LAS-X, v.3.7, Leica Microsystems). Acquisition setting were kept consistent throughout imaging across different experimental days ...
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bioRxiv - Bioengineering 2022Quote: ... The human-distal region skin tissue was cryoprotected with 30% sucrose and continually sectioned (thickness: 50 µm) using a cryostat (Leica CM 1950 ...
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bioRxiv - Biophysics 2024Quote: ... Proteins and vesicles were visualized on a laser scanning confocal microscope (Leica Stellaris 5) using 488 and 561 nm lasers for excitation for the proteins and lipids ...
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bioRxiv - Neuroscience 2019Quote: ... 5μm formalin fixed paraffin embedded tissue sections of human frontal cortex were deparaffinized and processed using heat induced epitope retrieval with an EDTA-based buffer (Leica #AR9640) for 20 minutes and incubated with primary antibody for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Cryo-samples prepared from single-tagged CLTA-GFP expressing SK-MEL-2 cells and incubated with Alexa Fluor® 647 ChromPure human transferrin (Jackson Immuno Research Inc.) were imaged on a Leica EM Cryo CLEM system (Leica). The system consists of a Leica DM6 FS widefield microscope that is equipped with a motorized Leica EM Cryo stage and a short working distance (<0.28 mm ...
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bioRxiv - Cancer Biology 2020Quote: ... In regions with CCF two to four consecutive cryosections with a thickness of 12 µm were put on membrane-slides (Leica Frame Slides, Nuclease and human nucleic acid free, PET-Membrane, 1,4 µm, Leica, Wetzlar, Germany) and incubated for one minute in 70% ethanol at −16°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody used for Dp427 was the anti-human Dystrophin form Leica Biosystems (1:25, Dy4/6D3, NCL-DYS1, RRID:AB_442080, Leica Biosystems, Wetzlar, Germany) and the Dystrophin antibody from Abcam (1:100 ...
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bioRxiv - Physiology 2024Quote: Human cells (A549 and BEAS-2B) were investigated using the TCS Sp5 Inverted Confocal laser scanning microscope (Leica Microsystems, Wetzlar, Germany) and a 20x /0.70 HC Plan Apochromat ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded tissues was carried out on a Discovery XT automated slide stainer using a mouse anti-human monoclonal antibody for FOLR1 (Novocastra Clone BN3.2; Leica Biosystems, Wetzlar, Germany) at 15 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Cell Biology 2020Quote: ... To compare the localization of the two proteins we used the SP8 STED microscope (Leica, with a 100× oil immersion objective and the LAS X Leica software) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein localization was visualized by a confocal laser scanning microscope (Leica TCS SP8; Leica Microsystems) with a 40X/1.30 oil immersion objective ...
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bioRxiv - Biochemistry 2022Quote: ... Protein localization was visualized by a confocal laser scanning microscope (Leica TCS SP8; Leica Microsystems) with a 40X/1.30 oil immersion objective ...
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bioRxiv - Cancer Biology 2021Quote: Formalin-fixed paraffin-embedded (FFPE) human skin tissue sections from organotypic tissue was stained for MITF (NCL-L-MITF, Leica Biosystems, Nussloch, Germany), MelanA (NCL-L-MITF ...
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bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Plant Biology 2020Quote: Cells expressing GFP-tagged proteins were examined using a Leica confocal microscope SP8X (Leica Microsystem, Germany) with 40×/1.0 water-dipping objective ...
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bioRxiv - Cell Biology 2022Quote: ... Protein location of GATA1 and p50 were detected by immune-florescence using Confocal Microscopy (Leica DMI6000). Nuclear protein was isolated from baseline Zfp467+/+ and -/- BMSCs lysate by Pierce™ NE-PER® Nuclear and Cytoplasmic Extraction Reagent Kit (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded whole-tissue sections from decalcified human bone marrow core biopsy specimens in a Bond RX automated tissue stainer (Leica Biosystems, Buffalo Grove, IL), as described previously91 ...
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bioRxiv - Systems Biology 2023Quote: ... paraffin-embedded whole-tissue sections from decalcified human BM core biopsy specimens in a Bond RX automated tissue stainer (Leica Biosystems, Buffalo Grove, IL), as described previously 80,81,87,88 ...
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bioRxiv - Neuroscience 2023Quote: ... Cryosections from human and mouse spinal cord or DRGs were cut at 12 μm using a freezing microtome (CM1850; Leica, Buffalo Grove, IL, USA) and incubated at 4°C overnight with primary antibodies including ...
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bioRxiv - Genetics 2021Quote: ... Protein localization was visualized by fluorescence confocal microscopy on a Leica SP5 microscope (Leica Microsystems, Wetzlar, Germany) and images were captured with LASAF as described previously [20].
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bioRxiv - Cancer Biology 2020Quote: ... Protein expression was quantified as positive pixels using Aperio Positive Pixel Count Algorithm software (Leica Biosystem, IL).
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bioRxiv - Cancer Biology 2020Quote: ... The expression of proteins was detected using the Bond Polymer Refine Detection kit (Leica Biosystems, Cat# DS9800) with diaminobenzidine as chromogen (88) ...
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bioRxiv - Neuroscience 2022Quote: ... The images of the targeting proteins were analyzed by high solution confocal microscopy (LEICA TCS SP8, Germany). Control results using only the 2nd antibodies were shown in supplementary Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein localization was analyzed 24-48 hours after infiltration by confocal laser scanning microscope (TCS SP8, Leica). GFP was excited at 488 nm and the fluorescence detected between 498-525 nm.
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bioRxiv - Cell Biology 2020Quote: Prior to staining for collagen type II the sections were blocked with Novolink protein (Leica Biosystems, Weltzlar, Germany) for 5 minutes at room temperature ...
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An annotation dataset facilitates automatic annotation of whole-brain activity imaging of C. elegansbioRxiv - Neuroscience 2019Quote: ... The fluorescence of the fluorescence proteins and the dye was observed sequentially using laser scanning confocal microscopy (Leica SP5 with 63× water immersion lens and 2× zoom) ...
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bioRxiv - Biochemistry 2021Quote: ... proteins fixed in 30% ethanol/15% acetic acid and the gel piece embedded in tissue embedding media (Leica). After careful mounting on a cryo-holder ...
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bioRxiv - Cell Biology 2024Quote: ... Cells that expressed the fluorescent fusion protein were identified using an epifluorescence microscopy (DM6000B, Leica Microsystems, Wetzlar, Germany) equipped with an oil immersion objective (1.4 NA ...
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bioRxiv - Immunology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with phospho-DRP1 (Ser 616 ...
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bioRxiv - Plant Biology 2021Quote: ... plant tissues expressing GFP-or RFP-fused proteins were imaged with a Leica TCS SP8 confocal microscope (Leica Microsystems) using the preset settings for GFP (Ex ...
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bioRxiv - Cell Biology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with the primary antibodies.