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Citations for Leica :
1 - 50 of 58 citations for Recombinant Human CD86 Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and processed using LASX single molecule detection analysis software (Leica LASX) after a bin of 4 (2x2 surrounding pixels ...
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bioRxiv - Microbiology 2020Quote: ... factor-VIII and ionized calcium binding adaptor molecule (IBA-1) (Leica Biosystems) or negative control slides primary antibody was replaced by a rabbit non-specific IgG isotype negative control antibody for 20 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... nanoparticle-tagged FITC and fluorescence-tagged immune cell populations were imaged using a Leica TCS SP8 Confocal Microscope (Leica Microsystems). DAPI ...
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bioRxiv - Neuroscience 2023Quote: CD86 and CD206 immunoreactivity were quantified in three fields per region of interest using a fluorescent microscope (40x magnification; Leica). Positive staining and co-localised staining were counted using CellProfiler with a MATLAB designated code ...
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bioRxiv - Cell Biology 2022Quote: ... Single Molecule Imaging was performed on a on a Leica SR-GSD 3D microscope (Leica Microsystems) as described in (Mateos et al ...
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bioRxiv - Neuroscience 2023Quote: ... Single-molecule fluorescence of labeled cells were captured using sequential laser scanning confocal microscopy (Leica SP8, Germany) and analyzed using Image J (NIH ...
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bioRxiv - Biophysics 2022Quote: The single-molecule measurements were conducted at a setup based on an inverted-widefield microscope (Leica DMI6000B) equipped with a high numerical lens (HCX PL APO 100x 1.47 OIL CORR TIRF) ...
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bioRxiv - Biophysics 2023Quote: ... The FLIM data acquisition was performed by high-precision single-molecule detection hybrid detectors (HyD SMD, Leica) with GaAsP photocathodes ...
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bioRxiv - Biophysics 2022Quote: ... Super-resolution localization images of CaV1.2 and CaV1.3 channel distribution were reconstructed using the coordinates of centroids obtained by fitting single-molecule fluorescence signals with a 2D Gaussian function using LASAF software (Leica). A total of 50,000 images were used to construct the images ...
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bioRxiv - Physiology 2023Quote: ... Fluorescence signals in each image were fit with a 2D Gaussian function which localized the coordinates of centroids of single molecule fluorescence within the LASAF software (Leica). Images were rendered at 20 nm/pixel (normalized Gaussian mode) ...
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bioRxiv - Neuroscience 2019Quote: ... the non-recorded ON was transferred in a different incubation chamber (Leica HI 1210) that provided similar incubation conditions to the recorded nerve while preventing exposure to ZD7288 and 8-Br-cAMP ...
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bioRxiv - Plant Biology 2020Quote: Cells expressing GFP-tagged proteins were examined using a Leica confocal microscope SP8X (Leica Microsystem, Germany) with 40×/1.0 water-dipping objective ...
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bioRxiv - Molecular Biology 2020Quote: ... images of DA GFP tagged neurons were captured with an inverted confocal microscope (Leica TCS SP8) using an x63 oil objective ...
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bioRxiv - Genetics 2022Quote: Cells expressing YFP and GFP-tagged Cut4p were visualized by confocal microscopy (Leica SP8 Confocal microscope). For visualizing ‘cut’ phenotype ...
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bioRxiv - Cell Biology 2021Quote: Images of immunostained cells or cells expressing fluorescent tagged proteins were acquired on Confocal inverted microscope SP8-X (DMI 6000 Leica). Optical sections were acquired with a Plan Apo 63x oil immersion objective (N.A ...
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bioRxiv - Cell Biology 2023Quote: ... as well as live tissues expressing fluorescently tagged proteins were imaged confocally using a Falcon SP8 laser scanning system (Leica Microsystems) with a 1.2-numerical aperture ...
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bioRxiv - Molecular Biology 2019Quote: ... after seeding and imaged regularly with a Leica DM IL LED system equipped with a HI PLAN I Phase 2 40x/0.50 (Leica) objective lens.
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bioRxiv - Cell Biology 2023Quote: ... and cell shapes were monitored by 3D brightfield microscopy (20X/0.30 HI Plan objective, DM IL LED microscope, Leica). Z-stacks were recorded ...
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bioRxiv - Cell Biology 2020Quote: Images were acquired using a Leica DM2000 fluorescent microscope and a Leica Hi-resolution Color DFC camera (Leica, Stockholm, Sweden) or EVOS M7000 automated imaging system (ThermoFisher).
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bioRxiv - Cell Biology 2020Quote: ... or human CD61 (clone 2f2, Leica Biosystems, Wetzlar, Germany). Sections were scanned on a Nanozoomer Digital Slide Scanner and analysed using NDP.view software (both from Hamamatsu Corporation ...
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bioRxiv - Biophysics 2019Quote: ... high resolution confocal images of membrane tagged GFP in live samples were collected using a 20x dry objective (Leica HCX PL APO; n.a. 0.70) on an inverted compound microscope (DMI6000 ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells expressing the fusion proteins eGFP-FAM76B and mCherry-tagged hnRNPA2B1 were visualized using a Leica TCS-SP8 confocal microscope (Leica Microsystems Inc., Shanghai, China).
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bioRxiv - Cancer Biology 2023Quote: ... with the following Abs: Anti-human Abs against CD19 (Leica #NCL-L-CD19-163 ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human CD163 mAb (clone, 10D6; Leica Biosystems, Buffalo Grove, IL), rabbit anti-mouse β-catenin pAbs (Proteintech ...
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bioRxiv - Immunology 2021Quote: ... Confocal images were acquired 24 h after hindbrain injection with the different recombinant S1 proteins on a widefield microscope (Leica THUNDER imager) with a 10x/0.32 objective using a DFC 9000 GTC sCMOS camera (Leica ...
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bioRxiv - Cell Biology 2022Quote: ... Human oocytes were imaged using a 63x water immersion objective (N.A1.20, Leica, 506346) with an incubation chamber maintained at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... The antibodies used were anti-human CD117 (EP10; Leica Biosystems, Buffalo Grove, IL), anti-human FcεRI (9E1 ...
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bioRxiv - Neuroscience 2023Quote: Human colonic tissues were embedded in paraffin using an embedding station (LEICA EG1150C) and sections (3 µm ...
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bioRxiv - Cell Biology 2020Quote: ... Muscle sections were also immunostained with human-specific spectrin (SPEC1-CE, 1:50, Leica) and human-specific lamin A/C (ab40567 ...
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bioRxiv - Genetics 2019Quote: Images of human and murine tissues were analysed using the SCN400 Slide scanner software (Leica). Figures 1-4 ...
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bioRxiv - Cell Biology 2020Quote: Imaging of the mouse and human sections was carried with the DM5500 fluorescent microscope (Leica Microsystems). Images were captured with the Leica application suite X version ...
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bioRxiv - Genomics 2021Quote: Fixed human brain tissue (prefrontal cortex, BA10) was sectioned at 50 m using a vibratome (Leica). The sections were boiled in IHC Antigen Retrieval Solution (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Attached RONAs and human cortical assemblies were sectioned (25 μm) by cryostat (CM3050; Leica, Nussloch, Germany) and collected on SuperFrost Plus glass slides (Roth ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed mouse brains and monkey or human brain samples were sectioned with a cryostat (model CM3050S, Leica) or a vibratome (Leica ...
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bioRxiv - Cell Biology 2020Quote: Western blot analysis for human iPSC–derived muscles was performed using antibodies to Dystrophin (NCL-DYS1, Leica), gamma-dystroglycan DAG1 (SC-165997 ...
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bioRxiv - Neuroscience 2022Quote: Paraffinized human optic nerves were cut in 5 μm sections using the microtome RM2155 (Leica, Wetzlar, Germany) and placed on 1 mm thick microscope slides (Marienfeld ...
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bioRxiv - Immunology 2023Quote: Platelet interactions with human monocytes were imaged in a Leica TCS SP5 SMD confocal system (Leica Microsystem). Cells were seeded in microslide 8-Well IBIDI chamber ...
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bioRxiv - Neuroscience 2022Quote: Human and mouse brain tissue was sectioned (30 μm-thick sections) using a SM2010 R sliding microtome (Leica) equipped with a BFS-3MP freezing stage and cooling unit (Physitemp ...
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bioRxiv - Developmental Biology 2022Quote: Images from mouse and human samples were acquired with a Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems). All images are shown as maximum intensity projections of acquired z-stacks of about one cell thickness (6-10 µm) ...
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bioRxiv - Immunology 2022Quote: ... serial human kidney sections were stained with anti-CD3 (LN10) or anti-CD8 (4B11; both Leica Biosystems, Chicago, IL) antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... automated methods on the Leica Bond Max autostainer using a mouse monoclonal anti-human CD3 antibody (Leica, clone LN10). Whole slide images of IHC stained slides were digitally captured using an Olympus IX83 microscope at 10x magnification and fixed exposure times ...
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bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
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bioRxiv - Cell Biology 2021Quote: Fixed and stained human pancreatic tissue sections were examined using a commercially available DragonFly200 spinning disk system (Andor) on a DMi8 microscope (Leica) with Plan Apo 40× NA 1.3 and Plan Apo 63× NA 1.4 oil immersion objectives ...
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bioRxiv - Cancer Biology 2021Quote: All staining of human tissues was performed on a Leica Bond RX automated stainer (Leica Microsystems, Inc., Buffalo Grove, IL). To ensure lectin specificity ...
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bioRxiv - Bioengineering 2022Quote: ... Bio SB) or anti-human CD163 (clone EP324, Bio SB) followed by an HRP-conjugated anti-mouse secondary antibody (Leica) and revealed with the peroxidase substrate 3 ...
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bioRxiv - Neuroscience 2023Quote: ... The entire human DRG section was imaged and merged using Leica Application Suite X (LAS-X, v.3.7, Leica Microsystems). Acquisition setting were kept consistent throughout imaging across different experimental days ...
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bioRxiv - Bioengineering 2022Quote: ... The human-distal region skin tissue was cryoprotected with 30% sucrose and continually sectioned (thickness: 50 µm) using a cryostat (Leica CM 1950 ...
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bioRxiv - Neuroscience 2019Quote: ... 5μm formalin fixed paraffin embedded tissue sections of human frontal cortex were deparaffinized and processed using heat induced epitope retrieval with an EDTA-based buffer (Leica #AR9640) for 20 minutes and incubated with primary antibody for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Cryo-samples prepared from single-tagged CLTA-GFP expressing SK-MEL-2 cells and incubated with Alexa Fluor® 647 ChromPure human transferrin (Jackson Immuno Research Inc.) were imaged on a Leica EM Cryo CLEM system (Leica). The system consists of a Leica DM6 FS widefield microscope that is equipped with a motorized Leica EM Cryo stage and a short working distance (<0.28 mm ...
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bioRxiv - Cancer Biology 2020Quote: ... In regions with CCF two to four consecutive cryosections with a thickness of 12 µm were put on membrane-slides (Leica Frame Slides, Nuclease and human nucleic acid free, PET-Membrane, 1,4 µm, Leica, Wetzlar, Germany) and incubated for one minute in 70% ethanol at −16°C ...