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Citations for Leica :
351 - 400 of 648 citations for Rat Orexin Receptor Type 2 HCRTR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The stained sections were observed within 1hr to detect callose deposition using confocal laser scanning microscopy (TCS SP5 2; Leica).
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bioRxiv - Neuroscience 2020Quote: ... Spinal cords were removed from the spinal column by hydraulic extrusion and embedded in low-melting point agarose (2-3%) before sectioning using a vibratome (Leica) into 300 µm thick coronal slices in ice-cold oxygenated ACSF ...
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bioRxiv - Neuroscience 2022Quote: ... brains were flash frozen in 2-methylbutane and stored at −20°C until sectioning at 40μm on a cryostat (Leica Biosystems) into cryoprotectant.
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bioRxiv - Immunology 2022Quote: Staining for SARS-CoV-2 antigen was achieved on the Bond RX automated system with the Polymer Define Detection System (Leica) used per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
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bioRxiv - Plant Biology 2021Quote: ... 6’-diamidino-2-phenylindole (DAPI) fluorescence signals were detected by confocal laser-scanning microscopy analysis using TCS SP8 (Leica Microsystems). All the images were obtained and processed with Leica LAS AF Lite software ...
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bioRxiv - Physiology 2021Quote: ... snap-frozen in 2-methylbutane on dry ice and sectioned into 10 μm slices using CryoJane Tape-Transfer System (Leica). Masson’s trichrome stain for cardiac fibrosis was performed according to the manufacturer’s instructions (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... Thin sections from the area of 2-photon imaging were cut with a diamond knife on an EM UC6 ultramicrotome (Leica), collected on pioloform-coated copper Synaptek slot grids (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 2 µL of the spore suspension per sample was then processed with Leica EM ICE HPF apparatus (Leica Microsystems), and then freeze substituted and embedded in Lowicryl HM20 (Electron Microscopy Sciences ...
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bioRxiv - Plant Biology 2022Quote: ... We cut the Spurr’s blocks into semi-thin sections (0.75–2 μm) with glass knives on an ultramicrotome Leica Ultracut UCT (Leica Microsystems) stained with a solution containing 1% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Images were captured using Metamorph software at every 15 minutes in 37C (PeCon Tempcontrol 37-2) using confocal spinning disk system (Leica DMI 6000 B equipped with YOKOGAWA CSU-W1 Confocal Scanner Unit ...
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bioRxiv - Neuroscience 2019Quote: ... Then a metal head plate was mounted (Narishige CP-2, Japan) to allow stereotaxic fixation under the two-photon microscope (Leica SP8 MP ...
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bioRxiv - Neuroscience 2019Quote: ... incubated in dichloromethane and then transferred to a clearing solution (benzyl alcohol: benzyl benzoate (1:2)) and imaged with a confocal laser scanning microscope (SP8, Leica).
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bioRxiv - Neuroscience 2021Quote: ... embedded in 2% LTE agarose/ Tris-buffered saline (TBS) and cut coronally in 50 µm sections on a vibratome (VT 1000S, Leica). Sections underwent antigen retrieval in a solution of 0.1 M sodium citrate (pH 6) ...
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bioRxiv - Immunology 2021Quote: ... Sections were serially imaged over the course of 1-2 hours and compiled using LAS X software (Leica Microsystems, USA). Image analysis was performed using Imaris software (Bitplane ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were then cryoprotected in 10% and 30% sucrose solutions for 2 hrs at RT and finally incubated in 30% sucrose and tissue embedding medium (Leica) (1:1 ...
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bioRxiv - Developmental Biology 2020Quote: ... 25% laser power (Pnmy-2nmy-2::gfp) or 13% laser power (Ppie-1mCherry::phPLCδ) was used (Leica TCS SP5-II). All analyses were performed on center Z-slices of Z-stacks through the NSMnb ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035 ...
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bioRxiv - Neuroscience 2022Quote: ... 2-photon imaging was performed using an upright microscope optimized for confocal and multiphoton microscopy (TCS SP5 II, Leica Microsystems). After the mouse was mounted to a stereotaxic holder (SR-AM and STS-A ...
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bioRxiv - Cancer Biology 2022Quote: ... with antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 1 or 2 respectively (AR9961, AR9640, Leica Biosystems). TMAs were blind-scored by multiplying staining intensity with staining coverage ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples and probes were co-denatured at 75°C for 2 min followed by incubation at 37°C overnight in a humidified chamber using ThermoBrite System (Leica). Slides were then washed for 2 min in 0.4x SSC at 75°C followed by a 30 second wash in 2x SSC at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Grids were post-stained for 30 min with 2% uranyl acetate (Roth) and 20 min with 3% lead citrate (Leica). TEM images were acquired using a Zeiss Leo 912 Omega (Zeiss ...
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bioRxiv - Neuroscience 2022Quote: ... the brain was cut into 2 mm thick coronal sections in 0.15 M cacodylate buffer using a vibratome (Leica VT1200). Then ...
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bioRxiv - Bioengineering 2022Quote: ... a ~ 2 nm layer of gold coating was completed using a sputtering machine (Leica EM ACE 600, Buffalo Grove, IL) and was imaged using an SEM (Apreo 2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC ...
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bioRxiv - Neuroscience 2023Quote: ... brains tissue fixed in 4% paraformaldehyde were cryoprotected in 30% sucrose for 2 hours followed by embedding in OCT for sectioning to 10μm thickness on a cryostat (Leica CM1860). Prior to staining ...
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bioRxiv - Immunology 2023Quote: ... as described previously.23 All heat induced epitope retrievals were performed with Bond™ Epitope Retrieval 2 (AR9640, Leica Biosystems) for 20 minutes at 95°C ...
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bioRxiv - Genetics 2023Quote: ... the worms were mounted on a 2% agarose pad and images were captured using a Leica compound microscope (Leica DM5000B).
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bioRxiv - Neuroscience 2023Quote: ... were imaged using a 0.75 NA 20X objective at 2 μm depth intervals on a Leica TCS SP8 laser scanning confocal microscope (Leica Microsystems). For the validation of the mouse line (assay # 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... Non-consecutive sections were cut with a thickness of 10μm and placed onto 2 capture areas of 10X Visium Spatial Gene expression slide using a cryostat (Leica CM3050S). The tissue Optimization kit was used to determine permeabilization times (24 min) ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were injected intravascularly in the duct of Cuvier with 27,6 nL of Membright Cy5-labeled tEVs or 4T1CD63pHluorinmScarlet-tEVs (at 10E10 EVs/ml) with a Nanoject microinjector 2 (Drummond) under a M205 FA stereomicroscope (Leica) as described previously (Mary et al. ...
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bioRxiv - Plant Biology 2023Quote: ... leaving around 2 mm of the root outside the needle which was submerged in a tissue-freezing medium (Jung, Leica) and quickly frozen in liquid nitrogen (Vogel-Mikuš et al. ...
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bioRxiv - Neuroscience 2023Quote: ... five µm section were cut followed by a Heat-Induced Epitope Retrieval at 100°C during 20 min in BOND Epitope Retrieval 2 (ER2) solution (Leica) with 0.1 Proteinase K before Deparaffinizing ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transgenic fish were screened at 2-4 dpf for CFP expression in the eyes using a CFP filter (Leica, 10447409) on a fluorescence stereomicroscope (Leica ...
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bioRxiv - Neuroscience 2023Quote: ... the animals were placed under 0.8-1% isoflurane anesthesia (2% used for induction) and imaged on an upright laser-scanning confocal (Leica SP8) or upright laser-scanning two-photon microscope (Bruker Ultima ...
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bioRxiv - Neuroscience 2023Quote: ... The caudal corpus callosa alongside adjacent tissue were punched with a 2 mm diameter punching tool and embedded in Epon (EM TP, Leica) prior to conventional embedding steps ...
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bioRxiv - Microbiology 2023Quote: ... The emitted fluorescence resulting from Fluo-8 or Rhod-2 AM staining was observed using a fluorescence microscope (DM6000B,Leica) with a 488-nm or 543-nm filter ...
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bioRxiv - Developmental Biology 2023Quote: ... dehydrated in methanol and cleared in Benzyl Alcohol/Benzyl Benzoate (1/2) prior to mounting and imaging with a SP8X confocal microscope (Leica). A 3D binary mask was built using Fiji to obtain a binary image of the tissue ...
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bioRxiv - Plant Biology 2024Quote: ... the eYFP fluorescence of fusion proteins was assayed 2–3 days after infiltration using a confocal microscope (Leica TCS SP8). For the Split–LUC assay ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030 ...
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bioRxiv - Cancer Biology 2020Quote: ... and BOND Polymer Refine Detection kit (DS9800, Leica Biosystems, Newcastle, UK) according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... Polymer detection system (Bond Polymer Refine Detection Kit #DS9800, Leica Biosystems) was used for horseradish peroxidase signal amplification ...
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bioRxiv - Cancer Biology 2022Quote: ... using the BOND Polymer Refine Red Detection Kit (Leica Biosystems, DS9390). Whole slide scanning was performed on an Aperio AT2 digital whole slide scanner (Leica Biosystems) ...
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bioRxiv - Immunology 2019Quote: ... The Bond Polymer Refine DAB Detection kit (Leica Biosystems, DS9800 DAB) was used and all procedures followed manufacture’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... Staining was visualized using the BOND Polymer Refine Detection kit (Leica Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems) as instructed by the manufacturer ...
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bioRxiv - Physiology 2023Quote: ... and staining using the Bond Polymer Refine Detection Kit (DS9800, Leica). Whole slides were then scanned using an Aperio AT2 slide scanner (Leica ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained with the Bond Polymer Refine Detection Kit (DS9800, Leica). The slides were scanned with a whole-slide scanner (Aperio AT2 ...
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bioRxiv - Cancer Biology 2023Quote: ... and hematoxylin (Bond Polymer Refine Detection Kit, Leica Microsystems, Cat# DS9800) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). Platinum replicas were floated off the glass by 5% hydrofluoric acid ...