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Citations for Leica :
201 - 245 of 245 citations for Rat Activity Dependent Neuroprotector Homeobox Protein ADNP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Slides were stained with the Bond Polymer Refine Red Detection kit using a Bond Rxm automated stainer (Leica Biosystems). Following a 20 min antigen retrieval in pH 6 citrate buffer (CLPP ...
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bioRxiv - Genomics 2020Quote: ... Single-molecule fluorescent mRNA in situ hybridization was conducted using RNAscope (Advanced Cell Diagnostics) probes and kits optimized for use on a BOND RX (Leica) system ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were stained using a Leica BOND-MAX-TM autostainer with BondTM epitope retrieval 1 or 2 and the Bond Refine Detection Kit (Leica). Primary antibodies are listed in Supplementary Table S1.
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bioRxiv - Cancer Biology 2020Quote: ... IHC technique was performed with commercial solutions from the Novolink™ Max Polymer Detection System Kit (Leica Biosystems, Newcastle UK). Before antibody incubation ...
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bioRxiv - Cell Biology 2021Quote: ... IHC staining was performed using the Novocastra Novolink™ Polymer Detection Systems kit (Code: RE7280-K, Leica, Biosystems, Newcastle, UK) as previously described 79 ...
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bioRxiv - Pathology 2021Quote: ... CA) was used with the Leica RNAScope 2.5 LS Assay-RED kit and a hematoxylin counterstain (Leica Biosystems, Richmond, IL). Slides were treated in protease (Advanced Cell Diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... with RNAscope 2.5 LS Assay Reagent Kit-Red (322150, Advanced Cell Diagnostics) and Bond Polymer Refine Red Detection (DS9390, Leica Biosystems). Control probes detecting a validated positive housekeeping gene (mouse peptidylprolyl isomerase B ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IHC at a 1:50 dilution for 30 minutes on a Bond RX auto-stainer using the Bond Polymer Refine RED Kit (Leica) minus Hematoxylin.
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bioRxiv - Cell Biology 2022Quote: ... The staining was examined using HRP Envision Systems (DAB Kit, MXB Biotechnologies) and analyzed using a dissecting microscope (Leica DM4000).
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bioRxiv - Pathology 2022Quote: ... IHC was performed using the automated BOND-RXm platform and the Polymer Refine Red Detection kit (Leica Biosystems, Wetzlar, Germany). Following automated deparaffinization ...
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bioRxiv - Pathology 2022Quote: ... with RNAscope 2.5 LS Reagent Kit-Red (322150, Advanced Cell Diagnostics, Newark, CA) and Bond Polymer Refine Red Detection (DS9390, Leica Biosystems). Control probes detecting a validated positive housekeeping gene (mouse Ppib to confirm adequate RNA preservation and detection ...
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bioRxiv - Cancer Biology 2023Quote: ... with RNAscope 2.5 LS Assay Reagent Kit-Red (322150, Advanced Cell Diagnostics) and Bond Polymer Refine Red Detection (DS9390, Leica Biosystems). Control probes detecting a validated positive housekeeping gene (mouse peptidylprolyl isomerase B ...
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bioRxiv - Cancer Biology 2022Quote: ... Hematoxylin/eosin (H&E) staining was performed with H&E Leica Kit Infinity for a full automated autostainer (Leica ST5020). IHC staining for Ki67 and MGP antibodies was performed using Bond III IHC autostainer for full Automated Immunohistochemistry (Leica biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... IHC was performed using the automated BOND-MAX and the Polymer Refine Red Detection kit (Leica Biosystems, Buffalo Grove, IL, USA), as previously described (Carossino et al ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: Genotype was determined by combined measurements of anogenital distance and fluorescent in-situ hybridization to detect X and Y chromosomes in interphase lymphocytes using the Kreatech KI-30505 kit (Leica Biosystems). Males also had their genotype verified by visual assessment of testis size ...
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bioRxiv - Genomics 2020Quote: ... Primary antibody was incubated for a certain period of time depending on its optimized protocol and a polymer detection system (Bond Polymer Refine Detection Kit [DS9800], Leica Biosystems) was used afterwards for the secondary antibody and horse radish peroxidase (HRP ...
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bioRxiv - Pathology 2022Quote: ... CA) was detected using the Leica RNAScope 2.5 LS Assay-RED kit with a hematoxylin counterstain (#322150, Leica Biosystems, Richmond, IL). An RNApol2 probe served as a host gene control to evaluate RNA quality ...
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bioRxiv - Bioengineering 2021Quote: ... Antigen retrieval was performed by incubating the sections in Leica Bond Enzyme solution (Bond Enzyme Pre-treatment Kit, Leica Biosystems, Germany) for 5 min ...
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bioRxiv - Immunology 2019Quote: ... antibodies were used for staining with Bond Refine polymer staining kit on the Bond Max automated staining system (Leica Biosystems, Germany). All stained slides were dehydrated in ascending ethanol and xylene washes before coverslipping with cytoseal (Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunohistochemistry was performed on a Bond RXm autostainer instrument with the Bond Polymer Refine Detection kit (Leica Biosystems, Buffalo Grove, IL) using the following antibodies and epitope retrieval (ER ...
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bioRxiv - Cell Biology 2022Quote: ... The immunostaining procedure was performed using a Leica BOND RXm automated platform combined with the Bond Polymer Refine Detection kit (Leica #DS9800). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 1 h in a 1:1 solution of 100% ethanol and solution A (Leica Historesin Embedding kit). After 2 h in solution A ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were loaded into the Bond Rx autostainer and the post primary steps were completed using the Bond Polymer Refine Detection Kit (Leica DS9800). For Ki-67 staining ...
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bioRxiv - Cancer Biology 2023Quote: ... the RNA unmasking conditions for the FFPE tissue consisted of 10-minute incubation with Proteinase K from the Bond Enzyme Pretreatment Kit at 1:1000 dilution (Leica Biosystems). HERV-K (Cat# DVF1-19321) ...
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bioRxiv - Bioengineering 2023Quote: ... The immunostaining procedure was performed using a Leica BOND RXm automated platform combined with the Bond Polymer Refine Detection kit (Leica DS9800). Sections on slides were dewaxed and rehydrated in deionized water and then pretreated with the epitope retrieval BOND ER2 high pH buffer (EDTA Based pH=9.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... We set up a sequential seven-colors multiplexed immunofluorescence by sequential staining cycles using the tyramide-signal amplification (TSA)-based OPAL seven-color Automation Research Detection Kit (Leica, DS9777) on the Bond RX automatic stainer (Leica Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Histological analysis of the 2 µm paraffin-embedded tissue sections was performed with the Bond Polymer Refine Detection Kit (Leica, Wetzlar, Germany) on a BondMax system (Leica) ...
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bioRxiv - Cancer Biology 2022Quote: ... five-micron formalin fixed paraffin-embedded sections were stained with Akoya Biosciences® Opal™ multiplex automation kit reagents (Leica Cat #ARD1001EA) on a Leica BondRX® autostainer ...
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bioRxiv - Pathology 2021Quote: ... antigen retrieval and immunohistochemical labelling were performed using the Bond III Autostainer and Bond Polymer Refine Detection Kit (Leica, Cat no. DS8900) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... NOS2 and COX2 expressions were also analyzed by fluorescent staining performed on the Leica Biosystems (Wetzlar, Germany) Bond RX Autostainer XL ST5010 using the Bond Polymer Refine Kit (Leica Biosystems DS9800), with omission of the Post Primary Block reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... NOS2 and COX2 expressions were also analyzed by fluorescent staining performed on the Leica Biosystems (Wetzlar, Germany) Bond RX Autostainer XL ST5010 using the Bond Polymer Refine Kit (Leica Biosystems DS9800), with omission of the Post Primary Block reagent ...
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bioRxiv - Microbiology 2019Quote: Purified RNA was stained with cyanine 3 or cyanine 5 using the ULS Fluorescent Labeling Kit for Agilent arrays from Kreatech (Leica Biosystems, Amsterdam, Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: PD-L1 staining was carried out with a Bondmax automated slide staining system using the Polymer Refine Detection Kit (both Leica Biosystems, Wetzlar, Germany) and a rabbit monoclonal anti-PD-L1 antibody (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... The staining procedure for HIF1α was carried out with the automated Leica BOND system using the Bond Polymer Refine Detection Kit (Leica Biosystems, Wetzlar, Germany).
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bioRxiv - Pathology 2021Quote: ... Slides were then stained with anti-Iba1 antibody (1:2000; Wako; 019- 19741 Richmond, VA) using the Bond Polymer Refine Red Kit (cat. DS9390 Leica Biosystem, Richmond, IL). The slides were stained using a pan-cytokeratin antibody (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... was performed using the Leica Bond Max Automatic Staining System in a peroxidase-mediated oxidation of 3,30-diaminobenzidine (DAB) from the Bond™ Polymer Refine Detection Kit (both from Leica Microsystems, Wetzlar, Germany). The negative control used was the Rabbit FLEX Universal Negative Control (cat ...
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bioRxiv - Immunology 2019Quote: ... 3,3’-Diaminobenzidine (DAB) chromogen was applied (10 minutes) (all reagents contained within the BOND Polymer Refine Detection kit, Leica Biosystems, catalogue no. DS9800). For double immunohistochemistry staining ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RNAscope staining was followed by the C219 IHC at a 1:200 dilution for 30 minutes on a Bond RX auto-stainer using the Bond Polymer Refine Kit (Leica, Buffalo Grove, IL) minus DAB & Hematoxylin ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... at 1:1500 dilution for 30 minutes followed by detection with horseradish peroxidase (HRP)-conjugated polymer system (Bond Polymer Refine Detection Kit, Leica, Concord, ON, Canada). All sections were then stained using 3,3’-Diaminobenzidine (DAB ...
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bioRxiv - Pathology 2021Quote: ... The RNAscope® ISH assay was performed using the RNAscope 2.5 LSx Reagent Kit (Advanced Cell Diagnostics, Newark, CA) on the automated BOND RXm platform (Leica Biosystems, Buffalo Grove, IL) as described previously (23) ...
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bioRxiv - Physiology 2019Quote: ... Fast red detection of mouse Gdf15 was performed on the Bond RX using the Bond Polymer Refine Red Detection Kit (Leica Biosystems, Cat No. DS9390) according to the ACD protocol ...
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bioRxiv - Physiology 2021Quote: ... Fast red detection of mouse Rxfp4 was performed on the Bond Rx using the Bond Polymer Refine Red Detection Kit (Leica Biosystems, Cat No. DS9390) with an incubation time of 20 min ...
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bioRxiv - Immunology 2019Quote: ... the above cycle was repeated twice with the first cycle using Fast red chromogen labelling (all reagents contained within the BOND Polymer Refine Red Detection kit, Leica Biosystems, catalogue no. DS9390) and the second cycle DAB chromogen labelling ...
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bioRxiv - Microbiology 2023Quote: ... and the RNAscope® ISH assay was performed using the RNAscope 2.5 LSx Reagent Kit (ACD) on the automated BOND RXm platform (Leica Biosystems, Buffalo Grove, IL, USA). Following automated baking and deparaffinization ...
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bioRxiv - Microbiology 2023Quote: ... for detection of Influenza virus A H5N1 nucleoprotein (NP) antigen was performed on the automated BOND RXm platform and the Polymer Refine Red Detection kit (Leica Biosystems, Buffalo Grove, IL). Following automated deparaffinization ...