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Citations for Leica :
301 - 350 of 457 citations for Mouse Protein SET SET ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Figures from double immunofluorescence labeling of GLP-1R and GFAP proteins were obtained on a Confocal Leica SP5 microscope (Leica Microsystems, France).
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bioRxiv - Cancer Biology 2020Quote: ... and BOND Polymer Refine Detection kit (DS9800, Leica Biosystems, Newcastle, UK) according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... Polymer detection system (Bond Polymer Refine Detection Kit #DS9800, Leica Biosystems) was used for horseradish peroxidase signal amplification ...
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bioRxiv - Cancer Biology 2022Quote: ... using the BOND Polymer Refine Red Detection Kit (Leica Biosystems, DS9390). Whole slide scanning was performed on an Aperio AT2 digital whole slide scanner (Leica Biosystems) ...
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bioRxiv - Immunology 2019Quote: ... The Bond Polymer Refine DAB Detection kit (Leica Biosystems, DS9800 DAB) was used and all procedures followed manufacture’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... Staining was visualized using the BOND Polymer Refine Detection kit (Leica Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems) as instructed by the manufacturer ...
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bioRxiv - Physiology 2023Quote: ... and staining using the Bond Polymer Refine Detection Kit (DS9800, Leica). Whole slides were then scanned using an Aperio AT2 slide scanner (Leica ...
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bioRxiv - Cancer Biology 2023Quote: ... and hematoxylin (Bond Polymer Refine Detection Kit, Leica Microsystems, Cat# DS9800) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained with the Bond Polymer Refine Detection Kit (DS9800, Leica). The slides were scanned with a whole-slide scanner (Aperio AT2 ...
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bioRxiv - Neuroscience 2022Quote: In vivo images of mouse retinal structure were obtained with spectral domain-optical coherence tomography (SD-OCT) (Leica Microsystems, Buffalo Grove, IL). The mice received 1% tropicamide drops to stimulate eye dilation and were anesthetized with ketamine and xylazine (50 and 5 mg/kg body weight ...
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bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121 ...
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bioRxiv - Neuroscience 2021Quote: ... and coronal sections of the mouse brain containing the dentate gyrus and prefrontal cortex were then cut using a VT1200S microslicer (300 μm) (Leica, Wetzlar, Germany). Micro-dissected areas were post-fixed at 4C for 2 hours in 2% osmium tetroxide in 0.1mol/l Cacodylate Buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded tissues was carried out on a Discovery XT automated slide stainer using a mouse anti-human monoclonal antibody for FOLR1 (Novocastra Clone BN3.2; Leica Biosystems, Wetzlar, Germany) at 15 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and incubated in blocking solution for 2.5h at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... The left and right hippocampus was micro dissected from genotyped P21 mouse brains using a laser-assisted capture microscope (Leica ASLMD instrument) with Leica polyethylene naphthalate membrane slides as described in ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.8% oxygen in an induction chamber (VetEquip, Livermore, CA) then placed in an Angle Two mouse stereotaxic frame (Leica Biosystems, Wetzlar, Germany) and secured with ear bars into a nose cone delivering isoflurane to maintain anesthesia ...
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bioRxiv - Biochemistry 2021Quote: ... Mouse brain tissue sections were sectioned at −21°C to a 12 μm thickness using a CM1850 Cryostat (Leica Microsystems, Wetzlar, Germany). Tissue sections were thaw mounted onto glass microscope slides (IMEB ...
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bioRxiv - Immunology 2020Quote: ... At least 150 cells per mouse were examined and quantified for cell type following the manufacturer’s instructions using a CME microscope (Leica, Buffalo Grove, IL) with a 40x objective ...
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bioRxiv - Neuroscience 2023Quote: ... A single 250 μm-thick parasagittal slice was cut for each mouse in this ice-cold solution using a sapphire blade (Delaware Diamond Knives, Wilmington, DE) on a vibratome (VT1000S; Leica, Deerfield, IL). Slices were then recovered at 32°C for 20-30 minutes in oxygenated artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2023Quote: ... in 0.8% oxygen in an induction chamber (VetEquip, Livermore, CA) and placed in an Angle Two mouse stereotaxic frame (Leica Biosystems, Wetzlar, Germany). Mice were given a subcutaneous injection of meloxicam (2 mg/kg ...
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bioRxiv - Cell Biology 2020Quote: Initial microscopic screening and quantitative examination of protein sorting in cultured mammalian cell lines was performed on a confocal Leica TCS SP5 II microscope (Leica GmbH, Heidelberg, Germany). Unless stated otherwise ...
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bioRxiv - Plant Biology 2019Quote: For BiFC assays Yellow Fluorescent Proteins (YFP) were visualized using a Leica TCS-SP2 laser scanning confocal microscope (LSM) (Leica Microsystems, Heidelberg, Germany) with a 63×1.4 NA oil-objective lens and processed using Leica confocal software v.2.61 about 16 h post-transformation ...
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bioRxiv - Cell Biology 2021Quote: ... the epithelium at ALI was visualized for Green Fluorescent Protein (GFP) at 20X by fluorescent microscopy using 3i Marianas/Yokogawa Spinning Disk Confocal Microscope (Leica Microsystems, TX, USA), assessed for functional phenotypes (as described below) ...
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bioRxiv - Microbiology 2023Quote: ... Concentrations of stock solutions of NP and MP beads were calculated using a hemocytometer and a Leica DM IL microscope with a green fluorescent protein (GFP) filter (Leica microsystems, Wetzlar, Germany) with an attached CoolLED pE-300 ultraviolet light system (Andover ...
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bioRxiv - Cell Biology 2019Quote: The gastrocnemius and quadriceps muscles were isolated from mouse hind limbs and dehydrated in a 30% sucrose solution and then sectioned in a constant cold section box (Leica CM 3000, Germany) at −20 °C ...
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bioRxiv - Physiology 2021Quote: ... blocked samples were incubated for 1 hr at room temp in buffer A containing mouse anti-dystrophin IgG1 (1:20, #NCL-DYS2, Novocastra / Leica, Buffalo Grove, IL). The sections were then washed 3 times for 15 min with PBS and incubated in buffer A containing Alexa 488 goat anti-mouse IgG1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Systems Biology 2023Quote: ... the mouse brains were sectioned coronally into 40 μm-thick slices at −18 to −20 °C using a microtome (Leica Microsystems, Wetzlar, Germany). Floating brain sections (fixed in 4% PFA ...
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bioRxiv - Cell Biology 2023Quote: Fresh mouse kidneys were embedded in low-melting agarose and cut into 300 μm slices using a vibratome (LEICA VT1200 S, Mannheim, Germany). Slices were maintained in carbogenated Krebs-Henseleit-Buffer.14 AKS were incubated with 5 μM propidium iodide for 5 min to label cells damaged by cutting.
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bioRxiv - Cancer Biology 2024Quote: Multiplexed immunofluorescence imaging: Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells expressing the fusion proteins eGFP-FAM76B and mCherry-tagged hnRNPA2B1 were visualized using a Leica TCS-SP8 confocal microscope (Leica Microsystems Inc., Shanghai, China).
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bioRxiv - Plant Biology 2024Quote: ... benthamiana leaves were agroinfiltrated to transiently express the protein of interest and examined with a Leica TCS SP5 Laser Scanning Confocal Microscope (Leica Microsystems, Exton, PA, USA) using a 63x water immersion objective NA 1.2 (HCX PL APO) ...
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bioRxiv - Immunology 2022Quote: ... The antibody was detected using Bond Polymer Refine Detection kit (Leica Biosystems) with diaminobenzidine (DAB ...
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bioRxiv - Physiology 2020Quote: ... and staining using the BondTM Polymer Refine Detection kit (DS9800, Leica Biosystems). Brightfield images were acquired using a Leica DM 1000 LED microscope and processed using Leica Application Suite software (Leica Biosystems).
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bioRxiv - Cancer Biology 2020Quote: ... before peroxidase block was performed using an Intense R kit (Leica, DS9263). Caspase 3 was stained at a previously optimised dilution of 1/500 and Ki67 at 1/100 before washing with Leica wash buffer and then application of rabbit envision secondary (Agligent ...
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bioRxiv - Immunology 2022Quote: ... Slides were counterstained with hematoxylin (Bond Polymer Refine Detection Kit, DS9800, Leica Biosystems.
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bioRxiv - Cancer Biology 2022Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica) for 5 minutes ...
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bioRxiv - Pathology 2021Quote: ... with the Bond Polymer Refine Kit (cat. DS9800 Leica Biosystem, Richmond, IL). Immunostaining was performed using the Bond RX automated system (Leica Biosystems ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: ... the slides were stained with the Bond Refine Detection Kit (Leica Biosystems), according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... For the IHC portion a Bond Refine staining kit (Leica Biosystems, DS9800) was used with a standard protocol minus the peroxide blocking step which was deleted ...
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bioRxiv - Cell Biology 2022Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica). FFPE sections were rinsed with wash buffer and Podxl antibody applied for 30 minutes (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... and detected with DAB using the HRP Polymer Refine Detection Kit (Leica) and hematoxylin counterstaining ...
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bioRxiv - Cancer Biology 2024Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica) for 5 min ...
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bioRxiv - Immunology 2023Quote: ... Detection was performed using the Bond Polymer Refine Kit (DS9800, Leica Biosystems). For dual staining ...
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bioRxiv - Cancer Biology 2023Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica). Sections for p21 had blocking solution applied from the Rat ImmPRESS kit (MP-7404 ...
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bioRxiv - Biophysics 2024Quote: ... with a bond polymer refine detection kit (Leica Biosystems, Milton Keynes,UK), and 3,3’-diaminobenzadine as a substrate ...
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bioRxiv - Molecular Biology 2021Quote: Facet joint cartilage and synovium (containing a thin layer of fibrous capsular tissue due to technical difficulties in obtaining pure synovium from mouse facet joints) were freshly dissected under a microscope (LEICA M80, with a LEICA IC80 HD video camera). Cartilage and synovium samples were collected separately in RNAlater solution (Ambion ...
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bioRxiv - Cancer Biology 2021Quote: ... The sections were incubated with CD8 mouse monoclonal antibody (clone 4B11, 1:150, 0.2 µg IgG2b/ml) from Novocastra (Leica Microsystems, Newcastle Upon Tyne, UK) for 45 minutes ...