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Citations for Leica :
151 - 200 of 396 citations for Mouse Protein DEK DEK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... followed by a polymer-labeled goat anti-mouse IgG coupled with alkaline phosphatase (30 minutes; Powervision, Leica Biosystems). Fast Red was used as the chromogen (15 minutes) ...
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bioRxiv - Developmental Biology 2022Quote: ... adult mouse brain tissue samples were embedded in 4% agarose and sections were cut using vibratome (Leica VT1200S). Until further processing sections were stored in ice-cold 1xPBS ...
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bioRxiv - Neuroscience 2020Quote: 300um thick coronal acute brain slices were prepared from P18-20 mouse using a virbrating microtome (Leica VT1200S) in ice cold cutting solution containing in mM (119 Choline Cl ...
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bioRxiv - Cancer Biology 2021Quote: The following primary antibodies were used: mouse anti-Synaptophysin (1:50, clone 27G12, Leica, NCL-L-SYNAP-299), rabbit anti-Chromogranin A (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... 300μm-thick coronal slices containing the SCN were obtained from PER2::LUC mouse brains using a vibratome (Leica) and placed on a semi-permeable membrane insert (PTFE ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse somatosensory cortical slices (350 μm) were prepared in the coronal plane using a vibratome (Leica 1200 V) in cutting solution ...
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bioRxiv - Pathology 2023Quote: ... Mouse is then placed into a petri dish and onto the stage of an inverted microscope (Leica, DMi8). 4 videos of 90 sec were acquired per mice and the number of contraction and dilatation (difference between maximum and minimum diameter ...
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bioRxiv - Neuroscience 2022Quote: Human and mouse brain tissue was sectioned (30 μm-thick sections) using a SM2010 R sliding microtome (Leica) equipped with a BFS-3MP freezing stage and cooling unit (Physitemp ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were stained manually with mouse anti-TTF1 (clone SPT24; TTF-1-L-CE, Leica, 1/400, 25’) and mouse anti-P40 (clone BC28 ...
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bioRxiv - Neuroscience 2023Quote: ... surface vasculature map was draw on a notebook for each mouse under microscope with light source (Leica M50) and put ‘*’ marker near the location of FOVs in this map for reference when doing the first imaging session ...
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bioRxiv - Neuroscience 2023Quote: ... samples of mouse brain were cut into 100 µm thick sections using a vibratome (Leica VT1200S, Vienna, Austria). Sections containing relevant regions of interest were rinsed in 0.15 M cacodylate buffer containing 2 mM calcium chloride 3 times for 10 minutes each followed by a secondary fixation in 1% osmium tetroxide ...
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bioRxiv - Neuroscience 2023Quote: The 100-µm thickness coronal sections of the fresh mouse brain were prepared by vibrating microtome (Leica VT1200) in in saturated O2 /Glucose+ artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Immunology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with phospho-DRP1 (Ser 616 ...
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bioRxiv - Plant Biology 2021Quote: ... plant tissues expressing GFP-or RFP-fused proteins were imaged with a Leica TCS SP8 confocal microscope (Leica Microsystems) using the preset settings for GFP (Ex ...
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bioRxiv - Cell Biology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with the primary antibodies.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were mounted once every 80 μm and mCherry fluorescent protein expression was detected under a microscope (Leica DMI6000). Microphotographs were taken at 2.5X magnification of each section where mCherry fluorescent signal was seen.
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bioRxiv - Developmental Biology 2022Quote: Images from mouse and human samples were acquired with a Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems). All images are shown as maximum intensity projections of acquired z-stacks of about one cell thickness (6-10 µm) ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 μm transverse zebrafish and mouse cryosections were collected on a Leica CM1900 crysostat (Leica Biosystems, Buffalo Grove, IL). 10 μm paraffin mouse sections were collected on a Thermo Scientific Shandon Finesse ME Microtome (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... transverse slices of 150µm from the hippocampi of a P17 mouse were prepared using a vibrating blade microtome (Leica) in cold (4°C ...
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bioRxiv - Developmental Biology 2020Quote: En face STED images of mouse cephalic explants were taken with a Leica SP8 TCS STED microscope (Leica Microsystems) equipped with a pulsed white-light excitation laser (WLL ...
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bioRxiv - Cancer Biology 2021Quote: ... A polymer detection system that includes an anti-mouse secondary antibody (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied in the tumor samples ...
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bioRxiv - Microbiology 2023Quote: ... FFPE mouse skin was processed and cut onto a polyethylene naphthalate (PEN) slide designed for LCM processing (Leica 11505158). At least 1,000 SGs or 1,000,000 μm2 of tissue was isolated to obtain enough material for RNA extraction ...
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bioRxiv - Cancer Biology 2023Quote: ... automated methods on the Leica Bond Max autostainer using a mouse monoclonal anti-human CD3 antibody (Leica, clone LN10). Whole slide images of IHC stained slides were digitally captured using an Olympus IX83 microscope at 10x magnification and fixed exposure times ...
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bioRxiv - Neuroscience 2023Quote: ... The mouse brains were then cut into 20 µm thick frozen tissue sections using a cryostat (Leica, Cat# CM1950) and placed on Superfrost Plus Adhesion Microscopic glass slides (Epredia ...
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bioRxiv - Microbiology 2022Quote: ... BondTM Polymer Refine Detection visualisation kit (Leica Byosystems, Germany) was afterwards used for signal detection using DAB as chromogen and counterstaining with hematoxylin.
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bioRxiv - Immunology 2022Quote: ... The revelation system (“Bond Polymere Refine” kit, DS9800, Leica) included a secondary antibody HRP (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... the BOND Polymer Refine Detection Kit (Leica Biosystems, UK) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... and the BOND Polymer Refine Detection kit (Leica Biosystems), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Images of immunostained cells or cells expressing fluorescent tagged proteins were acquired on Confocal inverted microscope SP8-X (DMI 6000 Leica). Optical sections were acquired with a Plan Apo 63x oil immersion objective (N.A ...
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bioRxiv - Biochemistry 2020Quote: ... Protein mixture (5 μL) were deposited onto the grids and blotted for 3 seconds using an Automatic Plunge Freezer (Leica).
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bioRxiv - Bioengineering 2020Quote: ... coli constitutively expressing the Green Fluorescent protein present on biofilms was examined by laser scanning confocal microscopy (Leica TCS SP8) using oil immersion at 40X magnification ...
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bioRxiv - Plant Biology 2023Quote: ... Each 3 µl of protein reaction mix was loaded into a flow-chamber and immediately observed with a confocal microscope SP5 (Leica) equipped with a 60x oil objective (NA 1.42) ...
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bioRxiv - Plant Biology 2024Quote: ... the eYFP fluorescence of fusion proteins was assayed 2–3 days after infiltration using a confocal microscope (Leica TCS SP8). For the Split–LUC assay ...
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bioRxiv - Plant Biology 2024Quote: ... Strains exhibiting mNeonGreen fluorescence were used to localize the RMT2-mNeonGreen fusion protein by TCS SP8 confocal laser-scanning microscopy (Leica); excitation/emission settings were 488 nm/515-530 nm using the HyD SMD hybrid detector for mNeonGreen ...
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bioRxiv - Neuroscience 2019Quote: ... They were then rinsed with PBS and one hemisphere for each mouse was sliced coronally at 100 μm with a vibratome (Leica) for imaging of dTomato-labeled abGC spines (Figure #) ...
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bioRxiv - Cell Biology 2019Quote: PFA-fixed adult mouse (3-4 month) brains were cut into 1-mm-thick sections using a vibratome (Leica, VT1200S) [11 ...
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bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed with TBST and incubated with PowerVision Poly-HRP anti-rabbit IgG or anti-mouse IgG (Leica Biosystems) in a humidified chamber at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Ten pg of the mRNAs was injected into fully grown mouse oocytes using an IM-9B microinjector (Narishige) under a Dmi8 microscope (Leica) in M2 medium containing 10 µM milrinone ...
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bioRxiv - Cancer Biology 2021Quote: ... for 8 minutes at room temperature (Poly-HRP-mouse anti-rabbit IgG, BOND Polymer Refine Detection System, Leica, Germany; DS9800). A color reaction with 3,3-di-amino-benzidine (DAB chromogen ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with a secondary antibody (rabbit anti-mouse IgG, BOND Polymer Refine Detection System, Leica, Germany, DS9800) for 8 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections from a series of 17 tumour samples from the validation cohort were stained for CD8 (mouse monoclonal 4B11, Leica Biosystems PA0183 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse or macaque slices (350 μm) were generated (Compresstome VF-300 vibrating microtome, Precisionary Instruments or VT1200S Vibratome, Leica Biosystems), with a block-face image acquired (Mako G125B PoE camera with custom integrated software ...
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bioRxiv - Genetics 2021Quote: Assessment of Aβ deposition was carried out by immunohistochemical staining of paraffin-embedded sections (3–5 μm-thick) with a mouse monoclonal (S6 F/3D) anti-Aβ antibody (Leica Biosystems Newcastle Ltd ...
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bioRxiv - Neuroscience 2022Quote: ... electroporated mouse pups were non-invasively screened at the age of P0 for cortical fluorescence using a fluorescence stereoscope (Leica MZ10f with X-Cite FIRE LED light source ...
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bioRxiv - Neuroscience 2022Quote: ... The mouse brains were frozen and sectioned sagittally with a thickness of 30 µm on a cryostat (Leica Biosystems, Germany), then the tissue slides were air dried for next step ...
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bioRxiv - Bioengineering 2022Quote: ... Bio SB) or anti-human CD163 (clone EP324, Bio SB) followed by an HRP-conjugated anti-mouse secondary antibody (Leica) and revealed with the peroxidase substrate 3 ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections of the perfused mouse brains were cryosectioned into 40μm at −20C using a Leica CM3050 cryostat (Leica Biosystems). In order to detect GFP expression via immunohistochemistry staining ...
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bioRxiv - Neuroscience 2023Quote: Ambra1 WT and Het female mouse brain were cut into coronal sections (30 μm) with Leica CM1950 instrument (Leica Biosystems). Sections were blocked with 10% normal horse serum (NHS ...