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Citations for Leica :
651 - 700 of 1082 citations for Mouse Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The abaxial side of cotyledons were imaged using a 63x/1.2 water immersion objective lens (Leica C-APOCHROMAT 63×/1.2 water). GFP was excited at 488 nm and fluorescence emission collected between 500-540nm ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Cell Biology 2021Quote: ... Samples were embedded at 60°C for 48 h and cut into 70 nm sections with a microtome (EM UC7, Leica, Germany). After electron staining with uranyl acetate and lead citrate ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were placed in an incubator chamber at 37 °C with 5% CO2 supplementation mounted on a Leica TCS SP8X microscope system (Leica Microsystems). Laser micro–irradiation was performed using a protocol adapted from (6) ...
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bioRxiv - Cell Biology 2022Quote: SMLM images were acquired at room temperature (24 °C) on a customized microscope (Mund et al., 2018) with a 160x NA 1.43 oil-immersion objective (Leica, Wetzlar, Germany). Illumination was done using a a LightHub laser combiner (Omicron-Laserage Laserprodukte ...
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bioRxiv - Neuroscience 2022Quote: ... It was then stored in 30% sucrose solution with 0.5% sodium azide at 4°C until preparation of 40 µm-thick coronal sections using a freezing microtome (SM 2010R, Leica Biosystems). Four to eight sections per mouse through the dorsal hippocampal region were used to evaluate hippocampal sclerosis by cresyl violet staining or gliosis by glial fibrillary acidic protein (GFAP ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then frozen in −20°C isopentane and cut into 20 μm-thick coronal sections on a cryostat (Leica CM1900), collected on silane-coated slides and stored at −20°C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Microbiology 2020Quote: ... were visualized in the phase-contrast mode with a Leica DMi8 microscope with a 20X objective and an attached environment control chamber (37°C with 5% CO2) (Leica Microsystems). For each Transwell ...
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bioRxiv - Bioengineering 2020Quote: ... and cells were cultured for 4 hours at 37 °C in the climate chamber of a Leica SP8 Confocal system (Leica Microsystems) microscope ...
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bioRxiv - Molecular Biology 2022Quote: ... temporal bones were immersed in 30% sucrose in PBS and stored at 4°C for 7 days before being cut into 14-16 micron thick sections on a cryostat (Leica CM3050). For each species ...
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bioRxiv - Systems Biology 2022Quote: FLIM of EGFR-mCitrine/PTB-mCherry or PTP-mCitrine/DyTo was performed at 37°C on the Leica SP8 laser-scanning microscope equipped with a fast lifetime contrast module (FALCON, Leica Microsystems) using the 63×/1.4NA oil objective ...
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bioRxiv - Cell Biology 2022Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Genomics 2022Quote: ... tissue slides were baked overnight at 60°C to ensure tissue adherence to the positively charged glass slides (Leica Bond Plus Microscope slides, Leica Biosystems). Then the tissue was deparaffinized and digested with Protease Plus (ACD Bio ...
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bioRxiv - Plant Biology 2019Quote: ... T1 plants were grown under long day conditions at 22 °C and phenotyped using the z-stack function of a Leica M205 FA microscope (Leica, www.leica.de).
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bioRxiv - Developmental Biology 2021Quote: ... For immunohistochemistry 5 μm thick serial sections were cut at −23°C using a Leica CM3050 cryostat (Leica Microsystems, Wetzlar, Germany) and collected in Superfrost® Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems). Images containing complexes were collected manually with a 120 kV TALOS transmission electron microscope (Thermo Fisher Scientific ...
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Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: ... and vitrification using a Leica GP2 plunger equipped with a blotting detection sensor and incubation chamber maintaining an environment of 21°C and 90% humidity (Leica Microsystems). Grids were placed into the GP2 incubation chamber and excessive liquid was manually removed with a piece of filter paper by gently touching the side of the grid ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA for 48 h at 4 °C and cut into 60 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Neuroscience 2022Quote: ... timelapse phase contrast images were acquired for 2 h at 37 °C with a 10X or 20X fluotar objective on a Leica DMIRE2 inverted microscope (Leica, Germany) equipped with a MS-2000 XYZ automated stage (ASI ...
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bioRxiv - Biochemistry 2022Quote: ... The blotting was carried out on the opposite side from the liquid drop and plunge-frozen in liquid ethane at −181 °C using an automated freeze plunging apparatus (EMGP, Leica, Germany). The samples were kept in liquid nitrogen and imaged using a Tecnai G2 (FEI ...
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bioRxiv - Biophysics 2022Quote: ... The blotting was carried out on the opposite side from the liquid drop and plunge-frozen in liquid ethane at –181 °C using an automated freeze plunging apparatus (EMGP, Leica, Germany). The samples were kept in liquid nitrogen and imaged using a Tecnai G2 (FEI ...
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bioRxiv - Biochemistry 2022Quote: ... polymerized at 70°C for 24 hours and then cut to 70 nm ultra-thin sections with an Ultracut S ultramicrotome (Leica, Germany). After cutting ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 s followed by high-pressure freezing after 25 s at -180°C under 2100 bar pressure in an HPM100 machine (Leica Microsystems). After freezing ...
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bioRxiv - Molecular Biology 2022Quote: ... the grid was back-blotted and immediately plunge frozen in liquid ethane at −182°C (Leica Automatic Plunge Freezer EM GP2).
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bioRxiv - Neuroscience 2022Quote: C57Bl/6J wild-type mice were anesthetized with isoflurane and brains were rapidly dissected and frozen on dry ice in OCT embedding medium and stored at -80°C until being sectioned on a cryostat (Leica CM3050s). Ten-micron sections were collected at -20°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Mouse brain was embedded and frozen in optimal cut temperature (OCT) (SAKURA, USA) compound at - 80°C and 10 μm cryosection slices were prepared by using a Leica cryostat (Leica, Germany). The slices were warmed up to room temperature and fixed with 4% PFA for 5 min before used ...
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bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with half seed in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems). with vacuum infiltration ...
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bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with dozens of seeds in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems) with vacuum infiltration ...
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bioRxiv - Plant Biology 2023Quote: ... they were kept at 28±1°C in dark for 16 h and observed under confocal microscope (Leica microsystems, TCS, SP5). BiFC for each combination was repeated twice and appropriate negative controls were used (Kudla and Bock ...
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bioRxiv - Neuroscience 2023Quote: ... containing the dorsal hippocampus were cut in ice-cold (4 °C) choline chloride ACSF using a Leica VT1000 vibratome (Leica Biosystems) and transferred to warm (33 °C ...
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bioRxiv - Neuroscience 2023Quote: ... sagittal sections at 5° angle tilted to the midline with 300 μm thickness were obtained inside the same slicing solution at 4°C using Leica VT1200S vibrotome (Leica Biosystems). Slices were immediately transferred to a chamber filled with artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Immunology 2022Quote: ... the tissue slides were heat pre-treated using a ER1 (pH6) target retrieval buffer for 15 minutes at 97°C (AR9961, Leica Biosystems). The slides were then stained for multiplex immunofluorescence using the BOND RX Opal multiplex procedure (v7.0.3.216 RX ...
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bioRxiv - Microbiology 2022Quote: ... Cryo-sections (70 nm thick) of the frozen material were obtained at −120°C using an Ultracut cryo-ultramicrotome (Leica Microsystems). The cryo-sections were collected on formvar-coated nickel grids ...
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bioRxiv - Developmental Biology 2023Quote: ... and embedded tissue was frozen rapidly and either stored at − 80 °C or immediately sectioned into 20-μm thick frozen sections using a cryostat (Leica CM3050S). Histological sections were placed in Superfrost® slides and placed at -20°C ...
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bioRxiv - Neuroscience 2023Quote: ... thin coronal midbrain cryosections (12 µm) were cut at -20°C and transferred to RNaseZAP-treated PPS FrameSlides (Leica Microsystems, # 11600294). Six sections per FrameSlide ...
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bioRxiv - Neuroscience 2023Quote: ... The L4 segments were fixed overnight at 4°C and cut into 60 μm thick transverse sections with a vibrating blade microtome (Leica VT1200). The sections were incubated for 3 days in rabbit anti-GFP (see Table S2) ...
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bioRxiv - Pathology 2023Quote: Carriers containing high pressure frozen samples for cryoFIB-SEM liftout were transferred to a cryo-ultramicrotome (Leica EM FC7, −160°C) equipped with a Trim 45 T1865 diamond knife ...
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bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Cell Biology 2022Quote: ... using the RNAscope™ Multiplex Fluorescent V2 Assay (Cat. No. 323270) with tissue pretreatment of 15 minutes at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 5 µg/mL for 20 minutes at 37 °C prior to live cell imaging by epifluorescence (Leica DMI6000B). Cells were counted as DAPI-positive if the nucleus showed bright staining and as apoptotic if there was characteristic nuclear fragmentation ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were placed in a 37°C chamber incubator supplemented with 5% CO2 mounted on Leica TCS SP8X microscope system (Leica Microsystems).
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bioRxiv - Cell Biology 2023Quote: The frozen blocks were removed from the-80°C freezer and allowed to sit in the cryostat (Leica CM 1950, #047742456) at-20°C for 1 hour to equilibrate ...
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bioRxiv - Neuroscience 2023Quote: The right hemisphere of each brain was sliced into 300μm coronal sections at −10°C using a cryostat (CM3050 S; Leica, Nuβloch, Germany). Punches of 0.75mm and 1.25mm were used to extract tissue from the frontal cortex ...
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bioRxiv - Bioengineering 2023Quote: ... the tissue was placed in a cryostat chamber at -20°C and sliced into 15 µm thick sections (CM1850, Leica microsystems). The FBR to the polymer coatings was assessed by staining with haematoxylin and eosin (H&E ...
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bioRxiv - Microbiology 2023Quote: ... as described above, the cells were incubated (37°C, 5% CO2) inside the Mica Microhub system using the internal incubator (Leica microsystems). Videos were analysed using the complementary software (Leica microsystems).
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bioRxiv - Molecular Biology 2023Quote: Adult flies containing P[GMR-Gal4] and four copies of P[UAS-arm*] or its variants were frozen at −20°C overnight and photographed with a Leica Stereo Dissecting Scope (Leica DMI6000B) attached to a digital camera ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in 4% paraformaldehyde overnight at 4°C, and sectioned coronally (thickness, 50–100 μm) using a vibratome (VT1200S, Leica Microsystems). Brain slices were incubated with 0.1% Triton X-100/phosphate-buffered saline for 10Cmin and blocked with Blocking-One P for 30 min ...