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Citations for Leica :
351 - 400 of 1218 citations for Mouse Chemokine C X3 C Motif Receptor 1 CX3CR1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Whole brains dissected from deeply anesthetized wild-type C57/BL6 mice were fresh frozen in Tissue-tek OCT media on dry ice and stored at −80 °C before being sliced into 20 µm slices on a CM 1950 Cryostat (Leica), mounted on SuperFrost Plus 25 × 75 mm slides (VWR) ...
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bioRxiv - Developmental Biology 2019Quote: ... EBs were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific) ...
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bioRxiv - Bioengineering 2019Quote: ... Japan) for observations under 25× magnification and by a digital camera on Leica microscope (DFC 420 C, Leica microsystems, Germany) for observations under 200× magnification ...
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bioRxiv - Developmental Biology 2021Quote: ... on dry ice and kept at −80°C until they were sectioned on a cryostat (OTF-5000, Bright Instruments and CM1900, Leica). Blocking solution (2% serum from the animal that the secondary antibody was raised in and 0.4% Triton-X 100 in PBS or 0.1% Tween (all from Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... brains were frozen in isopentane (−40°C) and sliced in serial coronal 40μm sections by using a cryostat (CM-1900, Leica, Germany). Samples were maintained in antifreeze solution before immunostaining procedure.
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bioRxiv - Bioengineering 2021Quote: ... Serial 12 µm sections were obtained by cryo-sectioning of the embedded intestine tissue at -20 °C using a cryostat (Leica). Cryosections were blocked with 5% normal donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with either 10 μM DHE or 5 μM MitoSOX for 30 min at 37°C in the dark and analysed by flow cytometry or in a DMi8 fluorescence microscope (Leica).
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bioRxiv - Cell Biology 2020Quote: ... The frozen samples were subsequently fractured under high vacuum in a pre-cooled freeze-fracture device BAF 060 at -130 °C (Bal-Tec/Leica). Fractured samples were then coated with 2.5 nm of tungsten at a deposition angle of 45° followed by an additional tungsten coating of 2.5 nm at 90° and afterwards transferred to a pre-cooled SEM (−120 °C ...
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bioRxiv - Genomics 2020Quote: ... overnight at 4°C with gentle rocking, then cryo-embedded in Optimal Cutting Temperature compound (OCT, Sakura) and sectioned using a cryostat (Leica) onto Superfrost Plus Microscope Slides (Fisherbrand) ...
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bioRxiv - Neuroscience 2021Quote: ... followed by immersion in 30% sucrose at 4°C sucrose and then embedded in an OCT block for cryosectioning at 35μm thickness (Leica, Germany) and used for histological studies.
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bioRxiv - Neuroscience 2020Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating sections with a vibrating microtome (Leica). Brain sections were kept at −20°C in a solution containing 300 mL.L−1 ethylene glycol ...
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bioRxiv - Biophysics 2022Quote: ... nobilis prisms from the prismatic layer of the pinna nobilis shell were fixed along the c-axis of calcite with DI water under -20°C then cut into slices with a thickness of around 30 µm using a Leica Microtome (Leica CM3050 S ...
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bioRxiv - Biochemistry 2022Quote: ... Images were acquired every 15 min at 30°C for 17 h using a Leica DMi-8 epifluorescent microscope fitted with a DFC365 FX camera (Leica) and Plan-Apochromat 63x oil objective (HC PL 614 APO 63x/1.4 Oil ph3 objective Leica) ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and c-FOS/5-HT immunolabeled cells were visually counted by an investigator blinded to samples using a light microscope (Leica DM2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal sections of the IC (200 μm) were cut in 34°C ACSF with a vibrating microtome (VT1200S, Leica Biosystems) and incubated at 34°C for 30 min in ACSF bubbled with 5% CO2 in 95% O2 ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were kept in PFA overnight at 4 °C and dehydrated in 30% sucrose (in PBS) for at least 18 hours before vibratome sectioning (VT1000s, Leica). Brains were cut into 50 µm coronal section using cryostat (CM 1950 ...
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bioRxiv - Neuroscience 2022Quote: ... Post-fixed brains were sliced in 35 μm thick sections in a -18°C chamber using a 3050 S Cryostat (Leica). Brain slices were either placed directly on Superfrost Plus slides (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal brain slices were taken at 40 µm using a cryostat at −20°C (Leica Biosystems Inc, Buffalo Grove, IL). The whole brain was collected ...
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bioRxiv - Molecular Biology 2023Quote: ... by deparaffinization and heat-induced epitope retrieval (HIER) at 100°C for 15 minutes using ER1 epitope retrieval buffer (Leica Biosystems product ...
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bioRxiv - Immunology 2023Quote: ... with a stage top incubator to maintain 37 °C and 5% CO2 or on a Leica TCS SP8 Laser Scanning Confocal (Leica) fluorescence microscope with an incubator to maintain 37 °C and 5% CO2.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Brains were removed and post-fixed in 4 % PFA in PBS at 4 °C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm ...
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bioRxiv - Microbiology 2023Quote: ... The samples were incubated overnight at 28°C and analysed with a Leica HCS LSI Macro Confocal (Leica Microsystems, Germany), using a 5x objective and GFP excitation/detection settings ...
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bioRxiv - Cell Biology 2023Quote: ... followed by determining the fluorescence lifetime of GFP in different cells cultured in a humidified chamber with 5% CO2 at 37 °C using a STELLARIS 8 FALCON (Leica) systems equipped with HyD X and HyD SMD detectors and an HC PL APO CS2 63x/1.40 OIL objective (Leica) ...
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bioRxiv - Molecular Biology 2023Quote: ... Frozen eyecups were sectioned to 40 μM thickness at -20°C on a Leica CM3050 S Cryostat (Leica, Wetzlar, Germany) onto TruBond 380 adhesive slides (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was snap frozen in OCT and stored at −70°C until sectioning in to 12 µm slices on a cryostat (Leica). Thaw-mounted sections were stored at −70°C ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... overnight at +4°C and then sedimented through 20% and 30% sucrose solution sequentially (minimum 24 hours each) before OCT (Leica) embedding ...
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bioRxiv - Biochemistry 2023Quote: ... Rat hippocampus tissue punches and thin sections were prepared according to published protocols.33 Rat brain hemisphere slices 16 µm thick were cut at -16°C using a cryostat (Leica CM3050 S ...
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bioRxiv - Neuroscience 2023Quote: ... five µm section were cut followed by a Heat-Induced Epitope Retrieval at 100°C during 20 min in BOND Epitope Retrieval 2 (ER2) solution (Leica) with 0.1 Proteinase K before Deparaffinizing ...
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bioRxiv - Neuroscience 2023Quote: Brain tissues after electrophysiology brain slice preparation were fixed in 4% PFA in 0.1 M phosphate buffer overnight at 4 °C and then were resected into 70–100 μm slices using a VT1000s vibratome (Leica Biosystems ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... The harvested tissues were then subsequently fixed with 4% paraformaldehyde (v/v) at 4°C and sliced into 40-μm sagittal and axial sections using a CM3050 S cryostat (Leica).
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bioRxiv - Microbiology 2023Quote: Chemically fixed EBOV-infected or transfected Huh7 cells on EM grids were vitrified by plunge-freezing into liquid ethane at −183 °C using a GP2 plunge-freezer (Leica) with a chamber temperature of 25 °C and 85% humidity ...
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bioRxiv - Microbiology 2023Quote: ... solution (0.1% uranyl acetate in anhydrous acetone) the samples were cooled to −90 °C and processed in an automated FS system (EM AFS2, Leica). After a washing step in acetone ...
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bioRxiv - Developmental Biology 2023Quote: ... ganglionic eminences explant cultures were placed in a humidified and thermoregulated chamber maintained at 37 °C and 5% CO2 on the stage of a spinning-disk microscope (Leica), equipped with an Orca Flash 4.0 Camera ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was then extracted and saved in 4% PFA overnight at 4 °C before being resected into 70 μm slices using a VT1000s vibratome (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... 40 μm slices were made of the entire dorsal hippocampus (dHPC) at -20 °C using a cryostat (CM1510 Leica Biosystems). Sections were stored individually in 96-well plates containing antifreeze solution (30% ethylene glycol ...
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bioRxiv - Biophysics 2023Quote: ... The nuclear envelope-positive area was assessed by thresholding the maximum intensity projection images of lamin A/C staining obtained by confocal microscopy (63x objective NA 1.25, Leica SP5) and merging them with nuclei masks ...
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bioRxiv - Bioengineering 2023Quote: ... The brains were extracted and postfixed for 24-48 hours in the same fixative at 4°C before being sliced into 50 μm coronal sections using a vibratome (Leica). The slices were blocked in 10% normal goat serum (SouthernBiotech ...
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bioRxiv - Neuroscience 2024Quote: ... then fixed overnight at 4 °C in 4% PFA and coronally sectioned at 35 μm intervals using a freezing microtome (CM3050S, Leica). Brain sections were permeabilized with a solution containing 5% bovine serum albumin and 1% Triton X-100 in PBS for 40 minutes at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The grids were then blotted for 3.0 s at 10°C and 75% humidity and plunge-frozen in liquid ethane using an EMGP2 (Leica, Germany). Cryo-EM data were collected with a 300 kV Krios G4 transmission electron microscope (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... front blotted for 3-4 seconds with 1 mm additional movement (95% humidity at 15°C) before being plunged into liquid ethane using an EM GP2 automatic plunge freezer (Leica). The cryo-EM data sets of NbE-µOR-Fab module complex were acquired on a Thermo Scientific Talos Arctica Cryo-TEM at an accelerating voltage of 200 kV ...
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bioRxiv - Neuroscience 2024Quote: ... Stained sections were stored at 4°C and imaged using the Inverted Leica DMIL LED Microscope with the Thunder imaging software (Leica).
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bioRxiv - Cancer Biology 2023Quote: ... anti-p53 (1:1000, Leica Biosystems, P53-PROTEIN-CM5), anti-p21 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-glial fibrillary acidic protein (1:400, Leica Biosystems); anti-Iba1 (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse monoclonal TTF-1 (clone SPT24, 1:100 pH6, NCL-L-TTF-1, Leica Novocastra), rabbit polyclonal anti-53BP1 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-MUC2 (1:50, Leica, NCL-MUC-2), mouse anti-CK17 (1:10 ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-TTF1 (Nkx2.1) (1:100; Novocastra; Leica Biosystems NCL-L-TTF-1), mouse anti-NeuN (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... prior to subsequent freeze substitution in acetone containing 0.4% uranyl acetate at −85 °C in an AFS freeze-substitution unit (Leica, Wetzlar, Germany). After gradient infiltration with increasing concentration of HM20 ...
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bioRxiv - Developmental Biology 2021Quote: ... EBs or brain tissues were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica, 14020108926). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems). The SARS-CoV-2 probe (catalog number 848568 ...
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bioRxiv - Cell Biology 2022Quote: ... Live-cell imaging was performed at 37°C with a Leica TCS SP8 confocal microscope with a 100×/1.40 Oil HC PL APO CS2 objective (Leica, Wetzlar, Germany) equipped with a white light laser ...