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Citations for Leica :
351 - 400 of 860 citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the grid was blotted for 1.5 s on the other side under 99% humidity and at 20 °C using EM GP2 plunge freezer (Leica) and immediately plunged into liquid ethane ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were imaged at 20 × (0.5 NA) magnification using a Leica DM6000 fluorescent microscope (Leica Microsystems GmbH, Wetzlar, Germany). Cell densities ...
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bioRxiv - Neuroscience 2023Quote: ... confocal images were acquired using a Leica SP8 laser-scanning microscope equipped with a 20× multi-immersion (NA 0.75; Leica), a 40× oil-immersion (NA 1.30 ...
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bioRxiv - Cell Biology 2023Quote: ... Blocks were equilibrated at -20°C for 1 h prior to cutting 12-µm sections using a cryostat (Leica Biosystems Inc. ...
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bioRxiv - Biophysics 2023Quote: Tiled images were captured using a Leica TCS SP8 laser scanning confocal microscope with a 20× oil immersion (NA 0.75) or a 10× air (NA 0.3) objective (Leica Microsystems). Images were merged and processed using LAS X (Leica Microsystems ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... The brains were then incubated in 20% sucrose and sectioned coronally on a sliding microtome (Leica SM 2000R, IL) at 35 µm (4 series equally) ...
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bioRxiv - Developmental Biology 2024Quote: Ultrathin sections (60 nm) of Lowicryl HM-20-embedded testes were cut on a UC-7 ultramicrotome (Leica Biosystems) with a diamond knife (Diatome ...
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bioRxiv - Plant Biology 2023Quote: ... Roots were taken and cryofixed in 20% BSA filled copper platelets (100 nm deep and 1.5 mm wide) with EM PACT1 high-pressure freezer (Leica). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... and the gap was imaged at different locations to acquire an image every 20 min using an inverted microscope (DMi8, Leica) equipped with an environmental chamber maintained at 37 °C and 5% CO2.
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bioRxiv - Developmental Biology 2021Quote: ... and the preparations were cleared in a series of increasing (20 to 80%) glycerol/1% KOH ratio until storage in 100% glycerin and photography (Leica MZ6 ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by a final step of fresh paraffin incubation for another 20 min at 65°C (Tissue Infiltration Medium by Surgipath available through Leica). The paraffin embedded tissue was sectioned in Richard-Allan Type 9 paraffin (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Serial 12 µm sections were obtained by cryo-sectioning of the embedded organoid at −20 °C using a cryostat (Leica). Cryosections were permeated with in PBST at temperature for 30 min and blocked with sheep serum (Zhongshanjinqiao ...
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bioRxiv - Cancer Biology 2020Quote: ... Representative images of each H&E section were captured using a Leica DM IL LED microscope equipped with a Leica MC170HD camera with a 20× objective using LAS EZ software (Leica). Scale bars = 20 μm.
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bioRxiv - Neuroscience 2021Quote: ... and stored at −80°C before being cut into 25 μm coronal sections in a cryostat at –20°C (CM3050S, Leica). Free-floating sections were washed with PBS ...
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bioRxiv - Immunology 2021Quote: Kidneys were isolated from resting mice after PBS perfusion and fixed O/N in 20 mL of 10% Neutral Buffered Formalin (Leica). Fixed kidneys were paraffin-embedded using a Shandon Citadel 1000 tissue processor (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Slides were scanned at a resolution of 20× magnification and pictures were taken using a Leica Aperio slide scanner (Leica). Quantification of collagen-afflicted areas on Masson’s Trichrome stained slides was performed using ImageJ software (National Institutes of Health ...
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bioRxiv - Neuroscience 2020Quote: ... Labeled grid-glued replicas received a final carbon layer (20 nm) onto the labeled replica side using a High Vacuum Coater ACE600 (Leica), followed by dissolution of the glue in Dynasolve 711 (Dynaloy ...
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bioRxiv - Biophysics 2020Quote: ... 1.2-NA water immersion objective or ×40 dry air or ×20 dry air on a laser scanning confocal microscope (TCS SP5; Leica). To label cell-cell adhesions (E-cadherin ...
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bioRxiv - Bioengineering 2020Quote: ... Trichrome stained slides were used to assess fibrosis at five-weeks post-injection using five evenly-spaced representative slides throughout the infarct scanned at 20× magnification using an Aperio ScanScope CS2 slide scanner (Leica). Non-nuclear blue staining was measured using the ‘Positive Pixel Count V9’ algorithm within ImageScope (Aperio ...
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bioRxiv - Neuroscience 2021Quote: ... Images were acquired with TCS SPE Leica confocal microscope using a 20-fold glycerol immersion objective (Leica Mikrosystems, Wetzlar, Germany) and analyzed with ImageJ as described in ref ...
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bioRxiv - Bioengineering 2021Quote: ... Serial 12 µm sections were obtained by cryo-sectioning of the embedded lungs tissue at -20 °C using a cryostat (Leica). Cryosections were blocked with 5% normal donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2020Quote: ... Adrenal gland slices were allowed to recover for 15 min at 37°C in bicarbonate-buffered saline in the presence of 0.2 mM (+)-tubocurarine and then kept at room temperature in the same solution before cryofixation in external cryoprotectant (20% bovine serum albumin in bicarbonate-buffered saline) using a HPM100 HPF device (Leica). Freeze substitution was performed as previously published (Rostaing et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then cryoprotected for 2h in 20% sucrose in PBS at 4°C and sectioned at 20 µm or 40-45 µm thickness with a sliding microtome (Leica). The tissues were then processed in function of the target immunofluorescence as described below.
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bioRxiv - Molecular Biology 2021Quote: ... The brains were sectioned sagittally into four series of 40-μm sections and two series of 20-μm sections with a cryostat (Jung CM3000 Leica). The 40-μm sections were mounted on glass slides for subsequent tissue dissection for total RNA extraction ...
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bioRxiv - Cell Biology 2021Quote: ... Brains were embedded and mounted in Tissue-Tek OCT compound (Sakura finetek) and 20 µm sections were cut using a cryostat (Leica). Brain sections were rehydrated in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... brains were flash frozen in 2-methylbutane and stored at −20°C until sectioning at 40μm on a cryostat (Leica Biosystems) into cryoprotectant.
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bioRxiv - Neuroscience 2022Quote: ... 11506243 were taken in 20 min intervals for 67 h with a DMC2900 color camera (both Leica Microsystems, Wetzlar, Germany), with cells kept at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence images were acquired with a confocal laser-scanning microscope (TCS SP8) using an HC PL APO CS2 20×/0.75 NA multiple-immersion lens (Leica Microsystems). The CGCs were identified based on NeuN signals and their morphological features ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Ten sagittal sections of 20 μm thickness were cut through the entire DRG using a cryostat (Leica, Richmond, IL, USA) and placed on poly-L-lysinated glass slides ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were embedded in gelatin/ albumin with 4% of glutaraldehyde and sectioned (20 μm) on a VT1000 S vibrating blade microtome (Leica). Sections were mounted in Fluoromount Aqueous Monting Medium (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... Z stack images (0.685 µm thick optical sections, 20-23 sections) were obtained with a confocal microscope (Leica SP8, Leica-Microsystems) using a 20x objective (HC PL APO CS2 ...
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bioRxiv - Microbiology 2019Quote: ... 20 images were acquired as 0.15 µM z-increment serial images using Leica Hyvolution Acquisition Software (Leica; Buffalo Grove, IL) with a Leica TCS SP8 confocal microscope equipped with a 100x/1.47 TIRF oil immersion objective lens and both white light laser (470nm-670nm ...
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bioRxiv - Neuroscience 2019Quote: Cryosections of 10 µm thickness were cut at −20°C in a cryostat (Leica Biosystems CM3050 S Research Cryostat, Leica Biosystems Nussloch GmbH ...
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bioRxiv - Neuroscience 2020Quote: Whole brains dissected from deeply anesthetized wild-type C57/BL6 mice were fresh frozen in Tissue-tek OCT media on dry ice and stored at −80 °C before being sliced into 20 µm slices on a CM 1950 Cryostat (Leica), mounted on SuperFrost Plus 25 × 75 mm slides (VWR) ...
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bioRxiv - Developmental Biology 2019Quote: ... EBs were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence images were acquired with a confocal laserscanning microscope (TCS SP8) using an HC PL APO CS2 20×/0.75 NA multiple-immersion lens (Leica Microsystems) or an APO CS2 100×/1.40 NA oil-immersion lens (Leica Microsystems ...
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bioRxiv - Neuroscience 2021Quote: ... and stored at −80 °C before being cut into 25-μm coronal sections in a cryostat at –20°C (CM3050S, Leica). Free-floating sections were washed with PBS ...
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bioRxiv - Bioengineering 2021Quote: ... Serial 12 µm sections were obtained by cryo-sectioning of the embedded intestine tissue at -20 °C using a cryostat (Leica). Cryosections were blocked with 5% normal donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2020Quote: ... A 250-µl dispase solution (2.4 U/ml) was then gently pipetted into the opening and imaging at 20× was started in an inverted microscope (DMi8, Leica) acquiring 1 image per minute for 45 min to 1 hr.
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bioRxiv - Cancer Biology 2021Quote: ... 20 random individuals were selected and imaged using a MZ16FA fluorescence microscope equipped with a DFC420C camera (Leica, Wetzlar, Germany). The microscope was set (exposure time and gain ...
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bioRxiv - Immunology 2022Quote: Imaging was performed on an inverted Leica TCS SP8 confocal microscope with white light laser and HyD photodetectors using an HC PL APO CS2 20 × /0.75 IMM objective (zoom factor of 1) (Leica Microsystems). Images were deconvolved using the LIGHTNING module in Leica Application Suite X (Leica Microsystems) ...
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bioRxiv - Cancer Biology 2022Quote: Brightfield whole slide images of hematoxylin and eosin-stained lung sections were scanned at 20-fold magnification by the UNC Pathology Services Core (PSC) using the Aperio AT2 digital scanner (Leica Biosystems Imaging ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed overnight in 4% PFA at 4°C and cryopreserved in sucrose prior to cryoembedding in NEG-50 (Epredia) and sectioning at 20 μm on a cryostat (Leica). Prior to staining ...
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bioRxiv - Cancer Biology 2022Quote: ... with antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 1 or 2 respectively (AR9961, AR9640, Leica Biosystems). TMAs were blind-scored by multiplying staining intensity with staining coverage ...
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bioRxiv - Biophysics 2022Quote: ... nobilis prisms from the prismatic layer of the pinna nobilis shell were fixed along the c-axis of calcite with DI water under -20°C then cut into slices with a thickness of around 30 µm using a Leica Microtome (Leica CM3050 S ...
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bioRxiv - Microbiology 2022Quote: ... Grids were post-stained for 30 min with 2% uranyl acetate (Roth) and 20 min with 3% lead citrate (Leica). TEM images were acquired using a Zeiss Leo 912 Omega (Zeiss ...
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bioRxiv - Pathology 2022Quote: ... Sections were mounted with DAPI Fluoromount-G (with Dapi, 0100-20, SouthernbBiotech) and imaged with an inverted SP8 confocal microscope (Leica). Elastin breaking number was calculated from hydrazide-stained sections ...
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bioRxiv - Neuroscience 2023Quote: ... 12μm cryosections (DRGs) and 18-20 µm cryosections (SC, skin, E15 and P0) were obtained using a standard cryostat (Leica).
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bioRxiv - Neuroscience 2022Quote: ... Coronal cryosections (20 µm) containing the AHA (bregma −0.45 to −1.35) were sliced using a Leica CM3050 S cryostat (Leica Biosystems Inc.) and sections were collected onto Superfrost Plus glass slides (VWR International) ...
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bioRxiv - Neuroscience 2022Quote: ... in vivo imaging was performed using Leica SP5 upright laser scanning microscope equipped with 20 × 1.0 NA water-immersion objective (all Leica Microsystems) and MaiTai Ti:Sapphire laser (Spectra-Physics) ...