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Citations for Leica :
51 - 100 of 493 citations for Mono 2 Carboxymethyl Hexyl Phthalate Cp 95% 13C4 99% Dehp Metabolite Iv 100Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... The grid was then plunge frozen in liquid ethane using an EM GP2 plunge freezer (Leica, 90-95% relative humidity).
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bioRxiv - Biophysics 2022Quote: ... The samples were then dehydrated using baths with increasing ethanol concentrations (50, 70, 95, 100%) and processed using a critical point dryer (CPD 300, LEICA). After being mounted on a sample holder ...
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bioRxiv - Neuroscience 2022Quote: ... Horizontal slices containing the substantia nigra were cut at 222 μm in Krebs buffer bubbled with 95/5% O2/CO2 using a vibrating microtome (Leica). Slices were allowed to recover at 30°C in vials with 95/5% O2/CO2 Krebs with MK801 (10 μM ...
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bioRxiv - Immunology 2022Quote: ... The grid was then plunge frozen in liquid ethane using an EM GP2 plunge freezer (Leica, 90-95% relative humidity).
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bioRxiv - Molecular Biology 2023Quote: ... front blotted for 3-4 seconds with 1 mm additional movement (95% humidity at 15°C) before being plunged into liquid ethane using an EM GP2 automatic plunge freezer (Leica). The cryo-EM data sets of NbE-µOR-Fab module complex were acquired on a Thermo Scientific Talos Arctica Cryo-TEM at an accelerating voltage of 200 kV ...
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bioRxiv - Microbiology 2023Quote: ... 95% humidity) and the sample was immediately frozen in liquid ethane in an EM GP automatic plunge freezer (Leica Microsystems). Grids were stored in liquid nitrogen until image acquisition.
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bioRxiv - Microbiology 2023Quote: ... The grid was then plunge frozen in liquid ethane using an EM GP2 plunge freezer (Leica, 90-95% relative humidity). Cryo-EM data were collected using a 300 kV FEI Titan Krios electron microscope (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 87 mM NaCl and 25 mM D-glucose) gassed with 95% O2/5% CO2 to give a pH of 7.4 using the VT-1200 Vibratome (Leica, Germany). Slices were then allowed to recover for at least an hour in a chamber filled with ACSF ...
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bioRxiv - Immunology 2022Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems). The SARS-CoV-2 probe (catalog number 848568 ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were rapidly removed from the skull and coronal brain slices containing the hippocampal formation (350μm thick) were prepared in the ice-cold cutting solution bubbled with 95%O2/5% CO2 using Vibratome VT1200S (Leica Microsystems) and then incubated in an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4 with 5% CO2/ 95% O2) and whole brain sagittal slices (300 µm) were cut using a vibratome (VT1200, Leica Biosystems). Afterwards slices were transferred to 34°C cutting solution for 45 minutes to recover and finally maintained at RT until used for recordings.
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bioRxiv - Neuroscience 2020Quote: ... and 25 mM dextrose) with bubbling 95% O2 and 5% CO2 using a vibratome (VT1000 S, Leica Microsystems, Buffalo Grove, IL). Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems). Images containing complexes were collected manually with a 120 kV TALOS transmission electron microscope (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... the brain was briefly submerged in ice cold cut solution bubbled with carbogen (95% oxygen, 5% CO2) and then sectioned into 300μm sagittal sections (Leica VT1200S vibratome) in bubbled ice-cold cut solution ...
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bioRxiv - Immunology 2019Quote: ... and 40 to 50 nm thin sections were cut with an LKB ultramicrotome UM IV (Leica) and analyzed using a CM10 electron microscope (Philips).
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bioRxiv - Neuroscience 2020Quote: ... Coronal brain slices containing the hippocampal formation (400 μm thick) were prepared in the ice-cold cutting solution bubbled with 95% O2/5% CO2 using Vibratome VT1200S (Leica Microsystems, Germany). The slices were transferred into an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Neuroscience 2021Quote: ... in coverslips expressing eGFP fusions of Xph clones or full-length PSD-95 were mounted in a Ludin chamber (Life Imaging Services) and transferred to an inverted microscope (Leica, DMI 6000B) maintained at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were allowed to rest in cold oxygenated (95% O2/5% CO2) aCSF and then sliced coronally using a vibratome (Leica VT1000 S). Brain slices (400-μm thick sections ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared in oxygenated (95% O2 / 5% CO2) dissection artificial cerebrospinal fluid (ACSF) at low temperature (3-4°C) using vibroslicer (Leica, VT 1200S). After preparation slices were recovered in a resting chamber (Harvard apparatus ...
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bioRxiv - Biophysics 2020Quote: ... stage of growth were imaged every 10 minutes for 12 hours in a customized incubator (37°C, 5% CO2, and 95% humidity) on a confocal microscope (Leica, TCL SP8), with acquisition of fluorescent and bright-field image z-stacks (Fig ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal and para-sagittal slices (300 μm) were prepared in ice-cold oxygenated (95% O2 / 5% CO2) ACSF with a vibratome (LeicaVT1000S, Leica Biosystems, Germany), then warmed to 36°C for 45 mins ...
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bioRxiv - Neuroscience 2020Quote: ... and 11 glucose saturated with 95% O2–5% CO2 and horizontal brain slices (150 μm thick) containing the VTA were prepared using a Vibratome (Leica Instruments, Nussloch, Germany). Slices were and allowed to recover at 35°C for at least 1 hr before recordings were initiated ...
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bioRxiv - Neuroscience 2021Quote: Experiments with the PSD-95-12-derived FRET reporter system were performed using the frequency domain analysis (LIFA) method a Leica DMI6000 (Leica Microsystems, Wetzlar, Germany) equipped with a confocal Scanner Unit CSU-X1 (Yokogawa Electric Corporation ...
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bioRxiv - Pathology 2022Quote: ... Heat-induced epitope retrieval (HIER) was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). Slides were treated in protease (Advanced Cell Diagnostics ...
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bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 20 minutes in sodium citrate-based ER1 buffer (Leica Biosystems, Richmond, IL) before immunostaining ...
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bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). The SARS-CoV-2 probe (cat ...
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bioRxiv - Neuroscience 2020Quote: ... equilibrated with 95 % O2-5% CO2) and parasagittal spinal cord slices (280 μm) were prepared with a vibratome (VT1200S, Leica Microsystems AG, Wetzlar, Germany) in the same solution ...
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bioRxiv - Neuroscience 2021Quote: ... immersed in ice-cold 95% O2/5% CO2-equilibrated sACSF and sectioned at 250 µm in the sagittal plane with a vibratome (VT1200S; Leica Microsystems Inc., IL, USA). Slices were then transferred to a holding chamber ...
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bioRxiv - Neuroscience 2023Quote: ... using a galvanometric beam scanner (Yanus IV; TILL Photonics) and a high numerical aperture objective lens (PL APO, 100X, oil, NA 1.4; Leica). The software Imspector (A ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were injected intravascularly in the duct of Cuvier with 27,6 nL of Membright Cy5-labeled tEVs or 4T1CD63pHluorinmScarlet-tEVs (at 10E10 EVs/ml) with a Nanoject microinjector 2 (Drummond) under a M205 FA stereomicroscope (Leica) as described previously (Mary et al. ...
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bioRxiv - Cell Biology 2022Quote: ... For sample vitrification, either a Vitrobot Mark IV (FEI, Hillsboro, OR) or a Leica EM GP2 (Leica Microsystems, Wetzlar, Germany) was used ...
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bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were seeded at 2000 cells/ml (total volume 2 ml) before starting the experiment at 37ºC supplemented with 5% CO2 in a live cell chamber (Leica DMI6000B with PEKON stage). Cells that attached and spread during the first two hours were used to quantify the changes in traction stresses during adhesion ...
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bioRxiv - Microbiology 2020Quote: ... Time-lapse imaging of the samples was performed in imaging medium (RPMI1640 without phenol red containing 10% FBS and 2 µg/ml lysostaphin) on a Leica TCS SP5 confocal microscope using a 40x (Leica HC PL APO, NA=1.3) or 63x (Leica HCX PL APO ...
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bioRxiv - Plant Biology 2021Quote: ... map70-5-c1 plants were crossed with the W131Y cell membrane marker line and homozygous F3 plants imaged and staged from stage I until stage IV (Leica SP8-MP DIVE 960 nm excitation). To determine total root and LRDZ length of ELTPpro>>PHS1ΔP in the UBQ10pro::EYFP:NPSN12 and map70-5 mutants ...
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bioRxiv - Microbiology 2023Quote: ... and 2% lead-citrate solution (Leica Ultrostain II ...
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bioRxiv - Neuroscience 2022Quote: ... in Epitope Retrieval Solution 2 (Leica, AR9640) for 20 minutes at 100°C ...
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bioRxiv - Neuroscience 2023Quote: ... and 3% lead citrate (Ultrostain 2, Leica).
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bioRxiv - Cell Biology 2024Quote: ... Epitope Retrieval Solution 2 (Leica Cat#AR9640), Retrieval Solution Low pH (Agilent Cat#GV80511-2) ...
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bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
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bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
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bioRxiv - Immunology 2022Quote: ... Approximately 2 μl of each sample was processed by HPF using a Leica EM PACT 2 (Leica, Milton Keynes, UK). After HPF ...
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bioRxiv - Biochemistry 2023Quote: ... Grids were glow discharged in air for 2 min and sample (2 μL) applied in an EM GP2 plunge freezer (Leica) at 4°C and 90% humidity ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then subjected to heat induced antigen retrieval and stained for anti-phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) and anti-KI-67 (CST #9027, 1/400 in PBS/2% BSA) on bond RX (LEICA). Appropriate negative and positive controls and isotype controls were included ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 Nucleoprotein: 1:2000 (Leica, AR9961) 30minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Bcl-2 (clone 3.1 mouse monoclonal antibody, Leica) was used at a 1:100 dilution with epitope retrieval solution 2 pre-treatment for 20 min.
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bioRxiv - Cancer Biology 2023Quote: ... using BOND Epitope Retrieval Solution 2 (Leica Biosystems). Detection was achieved using a Bond Polymer Refine Detection® DAB chromogen kit and counterstained with hematoxylin ...
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bioRxiv - Immunology 2023Quote: ... and BOND Epitope Retrieval Solution 2 (Leica AR9640) for MPO antibody ...
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bioRxiv - Pathology 2023Quote: ... speed 2 (Oxford) or 0.5 mm/s (Leica), and blade angle slightly below 0° (Oxford ...
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bioRxiv - Microbiology 2023Quote: ... Samples for milling were frozen on 2/2 200 gold mesh grids by plunge freezing in Leica EM GP2 or GP1 plunge freezer (Leica Microsystems, Germany) at 10°C and 90% relative humidity ...