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Citations for Leica :
51 - 100 of 101 citations for MAX Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... the sections were incubated with one drop of 3,3’ Diaminobenzidine (DAB) chromogen solution (cat.RE7270-K, Novolink MAX DAB, Leica Microsystems, Wetzlar, Germany) for 5 minutes ...
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bioRxiv - Immunology 2019Quote: ... antibodies were used for staining with Bond Refine polymer staining kit on the Bond Max automated staining system (Leica Biosystems, Germany). All stained slides were dehydrated in ascending ethanol and xylene washes before coverslipping with cytoseal (Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... Paraffin-embedded liver sections (2 μm or 7 µm for Sirius-Red staining) were processed for IHC staining using a BOND-MAX system (Leica, Germany) as previously described 54 ...
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bioRxiv - Microbiology 2020Quote: ... Max projections of confocal z-stacks (25 z steps spaced 1 μm) were acquired on a SP5 resonant APD (Leica, Wetzlar, Germany) at 1.7x zoom using a 63x glycerol objective (numerical aperture 1.4).
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bioRxiv - Cancer Biology 2021Quote: ... Formalin-fixed paraffin-embedded (FFPE) samples were cut to a thickness of 4 μm and stained on an automated stainer (BOND-MAX, Leica Biosystems, Germany) as part of the diagnostic routine in a clinically accredited histology lab ...
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bioRxiv - Cancer Biology 2023Quote: ... and staining processes based on the automatized “Polymer No Enhancer – Bond Polymer Refine IHC protocol no enhancer” protocol in Leica Bond Max (Leica Biosystems instrument).
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bioRxiv - Bioengineering 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Bioengineering 2021Quote: ... Sections were dried overnight and then subjected to immunohistochemistry (IHC) using a modified protocol F on the Bond-Max III fully automated staining system (Leica Biosystems, Wetzlar, Germany). All reagents from the Bond Polymer Refine Detection Kit DC9800 (Leica ...
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bioRxiv - Physiology 2020Quote: ... Two-four μm thickness sections from paraffin embedded samples were used for immunohistochemistry at the Tumour Bank of the HCB-IDIBAPS Biobank (IDIBAPS, Barcelona) using the Leica Microsystems’ Bond-max™ automated immunostainer together with the Bond Polymer Refine Detection System (Leica Microsystems, Spain). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Staining was performed using a Ventana Benchmark XT (Ventana Medical Systems, Inc., Tucson, AZ, USA) or Bond-Max autostainer (Leica Microsystems, Melbourne, Australia).
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bioRxiv - Molecular Biology 2019Quote: ... after seeding and imaged regularly with a Leica DM IL LED system equipped with a HI PLAN I Phase 2 40x/0.50 (Leica) objective lens.
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bioRxiv - Cell Biology 2023Quote: ... and cell shapes were monitored by 3D brightfield microscopy (20X/0.30 HI Plan objective, DM IL LED microscope, Leica). Z-stacks were recorded ...
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bioRxiv - Pathology 2022Quote: ... and incubated with a 1:200 dilution of anti-4-HNE and anti-AKR1A1 primary antibody overnight at 4°C primary antibody along with the Novolink™ Max Polymer Detection System (Leica Biosystems, Wetzlar, Hesse, Germany) and DAB substrate (3,30-diaminobenzidine ...
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bioRxiv - Cell Biology 2020Quote: Images were acquired using a Leica DM2000 fluorescent microscope and a Leica Hi-resolution Color DFC camera (Leica, Stockholm, Sweden) or EVOS M7000 automated imaging system (ThermoFisher).
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bioRxiv - Cell Biology 2020Quote: ... or human CD61 (clone 2f2, Leica Biosystems, Wetzlar, Germany). Sections were scanned on a Nanozoomer Digital Slide Scanner and analysed using NDP.view software (both from Hamamatsu Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... with the following Abs: Anti-human Abs against CD19 (Leica #NCL-L-CD19-163 ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human CD163 mAb (clone, 10D6; Leica Biosystems, Buffalo Grove, IL), rabbit anti-mouse β-catenin pAbs (Proteintech ...
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bioRxiv - Cell Biology 2022Quote: ... Human oocytes were imaged using a 63x water immersion objective (N.A1.20, Leica, 506346) with an incubation chamber maintained at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... The antibodies used were anti-human CD117 (EP10; Leica Biosystems, Buffalo Grove, IL), anti-human FcεRI (9E1 ...
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bioRxiv - Neuroscience 2023Quote: Human colonic tissues were embedded in paraffin using an embedding station (LEICA EG1150C) and sections (3 µm ...
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bioRxiv - Cell Biology 2020Quote: ... Muscle sections were also immunostained with human-specific spectrin (SPEC1-CE, 1:50, Leica) and human-specific lamin A/C (ab40567 ...
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bioRxiv - Genetics 2019Quote: Images of human and murine tissues were analysed using the SCN400 Slide scanner software (Leica). Figures 1-4 ...
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bioRxiv - Cell Biology 2020Quote: Imaging of the mouse and human sections was carried with the DM5500 fluorescent microscope (Leica Microsystems). Images were captured with the Leica application suite X version ...
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bioRxiv - Genomics 2021Quote: Fixed human brain tissue (prefrontal cortex, BA10) was sectioned at 50 m using a vibratome (Leica). The sections were boiled in IHC Antigen Retrieval Solution (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Attached RONAs and human cortical assemblies were sectioned (25 μm) by cryostat (CM3050; Leica, Nussloch, Germany) and collected on SuperFrost Plus glass slides (Roth ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed mouse brains and monkey or human brain samples were sectioned with a cryostat (model CM3050S, Leica) or a vibratome (Leica ...
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bioRxiv - Cell Biology 2020Quote: Western blot analysis for human iPSC–derived muscles was performed using antibodies to Dystrophin (NCL-DYS1, Leica), gamma-dystroglycan DAG1 (SC-165997 ...
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bioRxiv - Neuroscience 2022Quote: Paraffinized human optic nerves were cut in 5 μm sections using the microtome RM2155 (Leica, Wetzlar, Germany) and placed on 1 mm thick microscope slides (Marienfeld ...
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bioRxiv - Immunology 2023Quote: Platelet interactions with human monocytes were imaged in a Leica TCS SP5 SMD confocal system (Leica Microsystem). Cells were seeded in microslide 8-Well IBIDI chamber ...
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bioRxiv - Neuroscience 2022Quote: Human and mouse brain tissue was sectioned (30 μm-thick sections) using a SM2010 R sliding microtome (Leica) equipped with a BFS-3MP freezing stage and cooling unit (Physitemp ...
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bioRxiv - Developmental Biology 2022Quote: Images from mouse and human samples were acquired with a Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems). All images are shown as maximum intensity projections of acquired z-stacks of about one cell thickness (6-10 µm) ...
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bioRxiv - Immunology 2022Quote: ... serial human kidney sections were stained with anti-CD3 (LN10) or anti-CD8 (4B11; both Leica Biosystems, Chicago, IL) antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
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bioRxiv - Cell Biology 2021Quote: Fixed and stained human pancreatic tissue sections were examined using a commercially available DragonFly200 spinning disk system (Andor) on a DMi8 microscope (Leica) with Plan Apo 40× NA 1.3 and Plan Apo 63× NA 1.4 oil immersion objectives ...
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bioRxiv - Cancer Biology 2021Quote: All staining of human tissues was performed on a Leica Bond RX automated stainer (Leica Microsystems, Inc., Buffalo Grove, IL). To ensure lectin specificity ...
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bioRxiv - Bioengineering 2022Quote: ... Bio SB) or anti-human CD163 (clone EP324, Bio SB) followed by an HRP-conjugated anti-mouse secondary antibody (Leica) and revealed with the peroxidase substrate 3 ...
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bioRxiv - Neuroscience 2023Quote: ... The entire human DRG section was imaged and merged using Leica Application Suite X (LAS-X, v.3.7, Leica Microsystems). Acquisition setting were kept consistent throughout imaging across different experimental days ...
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bioRxiv - Bioengineering 2022Quote: ... The human-distal region skin tissue was cryoprotected with 30% sucrose and continually sectioned (thickness: 50 µm) using a cryostat (Leica CM 1950 ...
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bioRxiv - Neuroscience 2019Quote: ... 5μm formalin fixed paraffin embedded tissue sections of human frontal cortex were deparaffinized and processed using heat induced epitope retrieval with an EDTA-based buffer (Leica #AR9640) for 20 minutes and incubated with primary antibody for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Cryo-samples prepared from single-tagged CLTA-GFP expressing SK-MEL-2 cells and incubated with Alexa Fluor® 647 ChromPure human transferrin (Jackson Immuno Research Inc.) were imaged on a Leica EM Cryo CLEM system (Leica). The system consists of a Leica DM6 FS widefield microscope that is equipped with a motorized Leica EM Cryo stage and a short working distance (<0.28 mm ...
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bioRxiv - Cancer Biology 2020Quote: ... In regions with CCF two to four consecutive cryosections with a thickness of 12 µm were put on membrane-slides (Leica Frame Slides, Nuclease and human nucleic acid free, PET-Membrane, 1,4 µm, Leica, Wetzlar, Germany) and incubated for one minute in 70% ethanol at −16°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody used for Dp427 was the anti-human Dystrophin form Leica Biosystems (1:25, Dy4/6D3, NCL-DYS1, RRID:AB_442080, Leica Biosystems, Wetzlar, Germany) and the Dystrophin antibody from Abcam (1:100 ...
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bioRxiv - Physiology 2024Quote: Human cells (A549 and BEAS-2B) were investigated using the TCS Sp5 Inverted Confocal laser scanning microscope (Leica Microsystems, Wetzlar, Germany) and a 20x /0.70 HC Plan Apochromat ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded tissues was carried out on a Discovery XT automated slide stainer using a mouse anti-human monoclonal antibody for FOLR1 (Novocastra Clone BN3.2; Leica Biosystems, Wetzlar, Germany) at 15 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Cancer Biology 2021Quote: Formalin-fixed paraffin-embedded (FFPE) human skin tissue sections from organotypic tissue was stained for MITF (NCL-L-MITF, Leica Biosystems, Nussloch, Germany), MelanA (NCL-L-MITF ...
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bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded whole-tissue sections from decalcified human bone marrow core biopsy specimens in a Bond RX automated tissue stainer (Leica Biosystems, Buffalo Grove, IL), as described previously91 ...
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bioRxiv - Systems Biology 2023Quote: ... paraffin-embedded whole-tissue sections from decalcified human BM core biopsy specimens in a Bond RX automated tissue stainer (Leica Biosystems, Buffalo Grove, IL), as described previously 80,81,87,88 ...