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Citations for Leica :
551 - 600 of 706 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... ThKir and embWPC-ThKir) mice were perfused and directly processed to obtain images under the stereo fluorescent microscope (Leica MZ10 F). Coronal serial slices of 80 μm were obtained from TCA-GFP brains ...
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Laser Scanning Confocal printing, a new simple method for micro-device fabrication (LSCprint method)bioRxiv - Bioengineering 2024Quote: ... Tracking of how cells were being trapped was performed by live-image acquisition in a widefield fluorescent microscope (Leica DMI6000 B), using a low exposure time to have a real-time image ...
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bioRxiv - Neuroscience 2020Quote: ... we sliced the brain into 100 µm sections and imaged GFP and/or tdTomato fluorescence from every other slice using a 10x/0.4NA objective (Leica). Anatomical landmarks ...
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bioRxiv - Neuroscience 2021Quote: ... Coronal slices (300 μm thick) between 2.6 and 3.5 mm posterior to bregma were prepared using a vibrating blade microtome (Leica VT1200S). Slices were kept submerged in artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Biophysics 2021Quote: ... plunge-frozen in liquid nitrogen in 2.1 M sucrose and semi-thin sections (350 nm) were cut using the cryo ultramicrotome (UC6, Leica). Sections were picked up with a custom made metal loop in a droplet of 1% methylcellulose and 1.15 M sucrose and transferred to 35 mm glass bottom dishes (MatTek ...
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bioRxiv - Biophysics 2021Quote: ... plunge-frozen in liquid nitrogen in 2.1 M sucrose and semi-thick sections (400 nm) were cut using the cryo-ultramicrotome (UC6, Leica). Sections were picked up with a custom made metal loop in a droplet of 1% methylcellulose and 1.15 M sucrose and transferred to 35 mm glass bottom dishes (MatTek ...
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bioRxiv - Cell Biology 2022Quote: ... At least 20 cells/condition were imaged on either ELYRA PS.1 SIM/PAL-M LSM780 (Zeiss) using Plan-Apochromat 40x /1.4 oil immersion objective or DMi8 (Leica) with Lumencor SOLA SE led light (Lumencor ...
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bioRxiv - Bioengineering 2019Quote: ... with a 20 µm objective aperture diameter and a working distance at / around 2.5 mm (GeminiSEM 500, Zeiss; Cryo station VCT100, Leica). A sublimation step at −90°C was applied for 15 min in the SEM chamber to remove ice ...
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bioRxiv - Plant Biology 2021Quote: ... resin sections were stained with 0.05 % toluidine blue in 0.1 M 6.8 pH phosphate buffer (O’Brien et al., 1964) and visualized in a Leica DM 5000B microscope (Leica Microsystems) under bright field.
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bioRxiv - Plant Biology 2023Quote: ... The samples were washed with 0.05 M sodium cacodylate buffer and embedded in 4% agarose and sliced using VT1000S vibratome (Leica). For localization and auxin/cytokinin distribution pattern ...
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bioRxiv - Immunology 2021Quote: ... Images were taken using a Leica DM6000B fluorescent microscope, Orca Flash 4.0 mounted digital camera (Hamamatsu Photonics K.K., Japan) and LAS Advanced Fluorescence software (Leica Microsystems, Buffalo Grove, IL). Fluorescent channels were photographed separately and then merged ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were stained with phalloidin 647 and fluorescent z-stacks were acquired by confocal microscopy (Leica SP8, or Zeiss Examiner Z.1) at a pixel size of 60-70 nm ...
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bioRxiv - Bioengineering 2021Quote: ... The microplastics distribution was confirmed to be uniform on top of the bacterial lawn with a fluorescent microscope (Leica MZ10F fluorescence microscope, Leica, Wetzlar, Germany). For control experiments ...
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bioRxiv - Plant Biology 2020Quote: ... The expression of the eGFP marker of antibiotic-resistant roots that emerged was monitored by using fluorescent microscopic imaging (Leica stereomicroscope and imaging DFC7000 T Leica microscope camera) and four to ten independent roots showing expression of the marker were subcloned for each construct ...
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bioRxiv - Developmental Biology 2019Quote: ... To visualize the calcein and alizarin labels fluorescent images were taken (Olympus IX51 with Olympus DP70 camera or Leica TCS SPE Confocal). Following published standards(49) ...
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bioRxiv - Immunology 2021Quote: ... Images were taken using a Leica DM6000B fluorescent microscope, Orca Flash 4.0 mounted digital camera (Hamamatsu Photonics K.K., Japan) and LAS Advanced Fluorescence software (Leica Microsystems, Buffalo Grove, IL). Fluorescent channels were photographed separately and then merged ...
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bioRxiv - Microbiology 2022Quote: ... Cat# 897938-C3) using the RNAscope® LS Multiplex Fluorescent Assay (ACD, Cat# 322800) with a Bond RX auto-stainer (Leica Biosystems) with a tissue pretreatment of 15 minutes at 90°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections from electrophysiological or calcium imaging experiments containing the vH were examined with a fluorescent microscope (M205 FCA, Leica Microsystems, Wetzlar, Germany) using a 2.0x magnification objective ...
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bioRxiv - Cell Biology 2023Quote: ... recipients were anaesthetized using a previously described method (Dang et al., 2016) and imaged using a fluorescent stereo microscope (Leica M165 FC). Successful engraftment was defined as the presence of mylz2-GFP+/ mylz2-mCherry+ fibers ...
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bioRxiv - Biochemistry 2022Quote: ... The colorimetric change was instantly observed by unaided eyes,The fluorimetric analysis was then done under a fluorescent microscope (Leica DM 2500) at 490/535 nm (N2.1 filter).
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bioRxiv - Genomics 2022Quote: ... The crickets where then observed under a fluorescent dissecting microscope with digital camera attached (Leica M125, Leica Microsystems Inc., Deerfield, IL, USA) for either different eye coloration from wild type in knock-out treatments (using a standard white LED ring light ...
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bioRxiv - Physiology 2023Quote: ... Fluorescent images were captured with a Leica DM5500B microscope equipped with a motorized stage and a DFC360FX camera (Leica Microsystems, Wetzlar, Germany). The microscope was controlled by the OASIS-blue PCI controller and the Leica Application Suite X (LAS X) ...
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bioRxiv - Plant Biology 2023Quote: The transformant cells grown in liquid medium were collected at the logarithmic growth phase for the confocal fluorescent microscopy with TCS SP8 (Leica, Wetzlar, Germany). Chlorophyll autofluorescence was evaluated from the emission between 600 and 750 nm excited by a 552 nm laser ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... by the red fluorescence in the ocelli due to M{3xP3-RFP.attP}ZH-102D insertion when they are fathered by the male from GFP-labeled sperm lines using a fluorescent stereo microscope (Leica MZ10 F). For sperm counting experiments ...
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bioRxiv - Immunology 2024Quote: ... The quantifications (with a minimum of 300 cells for each condition) were performed with Leica DMI8 fluorescent microscopy (Leica Microsystems, Nanterre, France) using a 63X oil objective ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein-protein signal was scanned and scored digitally by a pathologist with Aperio image scope software (Leica Microsystems).
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bioRxiv - Cancer Biology 2021Quote: ... Macroscopic images of unpeeled tissue were obtained under Leica M80 zoom stereomicroscope with Leica Plan 1.0x Objective M-Series 10450167 coupled with Leica DFC295 Camera (Leica microsystems). Macroscopic tumors were punched and flash frozen in tissue freezing medium (Leica catalog no ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were cryoprotected with 30% sucrose in 0.1 M PB to prepare 50 μm-thick cryosections on a cryostat (CM1900; Leica Microsystems). All immunohistochemical incubations were performed at room temperature ...
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bioRxiv - Plant Biology 2020Quote: Plasmolysis was induced by mounting 6-day-old seedlings in 0.8 M mannitol on microscope slides and directly observed using confocal microscopy (Leica SP8). The proportion of the cell wall length in direct contact with the plasma membrane marker SYP122-mCitrine after plasmolysis was measured using Fiji after plasmolysis ...
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bioRxiv - Neuroscience 2020Quote: ... The immunofluorescent staining was performed on 40-µm thick free-floating coronal midbrain slice containing VTA and SNc DA neurons that were prepared using a vibratome (Leica VT1200S ...
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bioRxiv - Neuroscience 2022Quote: Images were collected from 2-3 consecutive sections 144 µm apart containing the central part of the demyelinating lesion using a TCS STED CW SP8 super-resolution microscope (Leica) and imported into NIH ImageJ software ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were cryoprotected with 30% sucrose in 0.1 M phosphate buffer to prepare 50 μm-thick cryosections on a cryostat (CM1900; Leica Microsystems). All immunohistochemical incubations were performed at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... and the area was calculated at 14 dpi using a Leica M 165C stereomicroscope equipped with Leica LASv4.3 image analysis software (Leica Microsystems). At 42 dpi ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was thoroughly washed in 0.1 M PBS and thereafter sectioned (40 µm thickness) using a vibratome (Leica VT1000, Leica Microsystems). The sections were stored in 1x PBS at 4°C.
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bioRxiv - Neuroscience 2022Quote: ... 300 μm coronal slices of the virus-injected area of the somatosensory cortex were made using a Leica VT1200 vibratome (Leica). After the recovery period ...
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bioRxiv - Neuroscience 2020Quote: ... Pulled glass pipette (tip inner diameter of 5–15 µm) filled with a tracer was stereotaxically inserted in the brain using Angle One stereotaxic instrument (myNeuroLab.com; currently Leica Instruments) and a small amount of the tracer (5–300 nL ...
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bioRxiv - Neuroscience 2020Quote: ... Spain) in 0,1 M phosphate-buffered saline (PBS) and sectioned using a vibratome (Leica VT 1000S, Leica Microsystems GmbH, Germany) on the frontal ...
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bioRxiv - Genomics 2021Quote: ... Sections of 10μm thickness were cut from the paraffine-embedded tissue blocks and mounted onto poly-ethylene naphtholate (PEN)-membrane slides (Leica). Tissue slides were stained with the following haematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2023Quote: ... 12μm cryosections (DRGs) and 18-20 µm cryosections (SC, skin, E15 and P0) were obtained using a standard cryostat (Leica).
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bioRxiv - Neuroscience 2022Quote: ... brains were rapidly removed and coronal slices (300 μm) containing the right AC were prepared in a cutting solution at 1 °C using a Vibratome (VT1200 S; Leica). The cutting solution ...
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bioRxiv - Neuroscience 2022Quote: ... the brain was cut into 2 mm thick coronal sections in 0.15 M cacodylate buffer using a vibratome (Leica VT1200). Then ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 μm/pixel) were captured from 100 to 300 frames through an objective lens (HCX IRAPOL 25×, NA = 1.00, Leica Microsystems) at a rate of 2.5 s per frame (i.e. ...
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bioRxiv - Neuroscience 2023Quote: ... it was washed three times with 0.1 M PB and cut along the sagittal plane using a vibratome (Leica VT2100S). Serial parasagittal sections (50 mm thick ...
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bioRxiv - Neuroscience 2023Quote: ... for 50-μm sections, the brains were embedded in 4% agar (01059-85, Nacalai Tesque) and sliced using a vibratome (VT1200S, Leica). For immunohistochemistry ...
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bioRxiv - Neuroscience 2023Quote: ... 300 µm thick coronal slices from the prefrontal and somatosensory cortices were obtained with a vibrating slicer (Microm VT1200 S, Leica) and incubated for at least 20 min at 38 °C in holding artificial cerebro-spicnal fluid (ACSF ...
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bioRxiv - Cancer Biology 2023Quote: ... 5µm thick sections were cut using a Leica RM2125 RTS microtome and sectioned onto Poly-L-lysine coated slides (Leica Surgipath Apex Superior adhesive slides ...
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bioRxiv - Neuroscience 2023Quote: ... acute V1 slices (400-μm thick) from P28–32 (bath-application) and P27–42 (uncaging) mice were cut with a vibratome (VT 1200, Leica) in ice-cold slicing solution containing (in mM ...
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bioRxiv - Neuroscience 2023Quote: ... was cryoprotected with 30% sucrose in PBS 0.1 M for 48 h and cut afterwards in 50-µm-thick coronal sections using a sliding microtome (LEICA SM2000R, Leica). Slices were collected in 6 subseries and stored at −20°C in a cryoprotective solution (30% glycerol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Images were taken with a digital camera (Canon EOS Rebel SL3, Canon USA) attached to a stereomicroscope (Leica M-Series). The total length of each copepod (from anterior of eye spot to fork in caudal furca ...
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bioRxiv - Neuroscience 2023Quote: ... fitted with a CMOS camera with 3.45 μm/pixel resolution (Basler ace acA2440-75 m) and a 0.63x objective lens (Leica Planapo 0.63x). Alternating dual light illumination was generated using an OptoLED system (Cairn Research ...