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Citations for Leica :
201 - 225 of 225 citations for Lifeink 200 Collagen Bioink since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The brain tissue containing CN was sliced into 200-300 µm thick sections parasagittally in the NMDG solution with a microtome (Leica, VT1200). The slices were transferred into the oxygenated NMDG solution (34 ± 0.5 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Rabbit mAb (Mouse Preferred; IHC Formulated) (CST, #12202)) was used 1:200 and developed with Bond Polymer Refine Detection (Leica, DS9800).
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bioRxiv - Cell Biology 2024Quote: ... Cells were pelleted by centrifugation at ∼800 RCF for five minutes and high-pressure frozen in the 200-µm recess of an aluminum platelet (Engineering Office M. Wohlwend 241) using an HPM100 (Leica Microsystems). The samples were then freeze substituted in 0.1% uranyl acetate ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was blocked in 2% agar and 200 µm thick coronal sections were cut using a vibratome (Leica VT 1000 S). The slices were collected on gelatin-coated glass slides and stained with solution D and E before being dehydrated through graded alcohols and mounted using Permount (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2019Quote: ... Transverse slices of the auditory brainstem were cut at a thickness of 200 μm using a vibratome slicer (VT1000; Leica, Bannockburn, IL), and incubated at 37°C for 30 min in normal aCSF and thereafter kept at room temperature (22-24°C ...
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bioRxiv - Cancer Biology 2021Quote: Larvae fixed in 4% PFA overnight at 4°C were embedded in 4% low melting temperature agarose and transverse sections collected at 200 µm intervals using a vibrating microtome (Leica VT 1000S). Sections were blocked with 1% BSA in PBS/0.3% Triton X-100 and incubated with a 1:750 dilution of mAb414 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... brains were then transferred to a 30 % sucrose solution for at least 3 days and coronal sections (200 µm) containing hippocampus were obtained using a vibratome (Leica vt 1000S). Sections were collected into 24-wells plate in sucrose 6% for one day stored at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Semi quantitative analysis was done by counting three independent microscopic fields (n=100-200 cells/field) for staining of specific antigen using upright microscope (Leica DM1000, Germany). Tissue array (US Biomax ...
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bioRxiv - Neuroscience 2022Quote: ... Tiled scans of individual whole sections were prepared using a x20 objective and the scanning function of a Leica Aperio Versa 200 Microscope (Leica, Wetzlar, Germany) available in the UCLA Translational Pathology Core Laboratory.
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bioRxiv - Systems Biology 2022Quote: ... were dropped under paraffin oil and their circumference measured by formula using the eye-piece graticule (10 mm = 200 parts) (Leica Microsystems, Germany). A 5μl drop was pulled around the midgut portion and MTs and a 0.3μl drop onto the CNC with the help of a capillary pull glass rod ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm segments from the root tip were embedded in 3% agarose and were sectioned at 150-200 μM using a vibratome (Leica VT1000 S). The sections were stained in Clearsee buffer (Ursache et al ...
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bioRxiv - Plant Biology 2022Quote: ... The roots were washed in water and one cm segments from the root tip were embedded in 3% agarose and made sections of 150-200 μM using a vibratome (Leica VT1000 S). Confocal Laser Scanning microscopy was performed on a Zeiss LSM700 confocal with the 20X objective ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were mounted on stubs using colloidal silver as an adhesive and sputter coated with 6 nm gold (Leica Coater ACE 200). Cells were imaged using FEI Quanta 3D scanning electron microscope operated at an accelerating voltage of 2 kV ...
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bioRxiv - Cell Biology 2023Quote: ... the block was cut to a 25-nm thickness to create a ribbon of 50–200 serial sections using a ultramicrotome (EM UC7, Leica, Wetzlar, Germany) equipped with a diamond knife (Ultra JUMBO ...
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bioRxiv - Microbiology 2023Quote: ... Samples for milling were frozen on 2/2 200 gold mesh grids by plunge freezing in Leica EM GP2 or GP1 plunge freezer (Leica Microsystems, Germany) at 10°C and 90% relative humidity ...
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bioRxiv - Physiology 2023Quote: ... Particles were manually offered to the coral fragments in individual feeding chambers (200 mL) under a stereomicroscope (Leica Microsystems GmbH, Leica EZ4, Germany). The microdebris was sampled from the treatment tanks to provide particles covered with a biofilm ...
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bioRxiv - Cell Biology 2023Quote: ... Ribbons of serial ultrathin sections (400×200 μm, 80 nm thick, ∼250 sections) were made using a Leica Artos 3D Ultramicrotome (Leica AT4 knife). The ribbons were placed on ITO glass and coated with 5 nm carbon (Leica ACE600 ...
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bioRxiv - Neuroscience 2021Quote: ... The brain was removed and coronal slices (200 μm) containing either the parabrachial nucleus (PBN) or the periaqueductal gray (PAG) were sectioned on a vibratome (Leica VT1200S, Wetzlar, Germany) in ice-cold oxygenated sucrose solution (in mM) ...
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bioRxiv - Biophysics 2022Quote: ... transferred to W5 buffer, and frozen on a glow-discharged grid (AG150P 200 mesh Cu, Zhongjingkeyi Technology) using a high-pressure freezer (Leica HPM100, Leica Microsystems). The grid was then transferred to Leica UC7+FC7 (Leica Microsystems ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RNAscope staining was followed by the C219 IHC at a 1:200 dilution for 30 minutes on a Bond RX auto-stainer using the Bond Polymer Refine Kit (Leica, Buffalo Grove, IL) minus DAB & Hematoxylin ...
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bioRxiv - Neuroscience 2022Quote: Temporal lobe tissue fixed with 2.5% glutaraldehyde-2% PFA in 0.1 M phosphate buffer (PB) was transversely sectioned at 200 µm using a Leica VT1200S vibratome (Leica Microsystems GmbH, Heidelberg, Germany). Slices were further post-fixed in 2% osmium tetroxide in 0.1 M PB for 1.5 hours at room temperature ...
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bioRxiv - Physiology 2023Quote: ... Particles were manually offered to the coral fragments in individual feeding chambers (200 mL) under a stereomicroscope (Leica Microsystems GmbH, Leica EZ4, Germany). The microdebris was sampled from the treatment tanks to provide particles covered with a biofilm ...
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bioRxiv - Neuroscience 2023Quote: ... the brains were quickly removed from the skull and cut into 200 µm thick coronal slices using a Leica vibratome VT 1200S (Leica Biosystems, Buffalo, IL, USA). The slices were then incubated for 30 minutes at 34 °C in the isolation NMDG buffer and for another 30 minutes in artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: to detect muscle fibre types we used mouse anti- myosin heavy chain (slow) (Leica, clone WB-MHCs; 1:200); to delimit the fibres we used rabbit anti-collagen type VI (Abcam ...
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bioRxiv - Pathology 2023Quote: ... Diluted primary antibody (CST #8242 1:800; CST #99940, 1:150; CST #9718, 1:500; Abcam ab107099, 1:X; Leica P53-CM5P-L 1:200) was applied and slides were incubated at 4°C overnight ...