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Citations for Leica :
101 - 150 of 160 citations for L Valine 13C5 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Slides were stained manually with mouse anti-TTF1 (clone SPT24; TTF-1-L-CE, Leica, 1/400, 25’) and mouse anti-P40 (clone BC28 ...
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bioRxiv - Biophysics 2023Quote: Widefield fluorescence images were acquired using 20X air objective (HC PL FLUOTAR L PH1 20x, NA=0.4, Leica) mounted on a Leica DMi8 microscope ...
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bioRxiv - Neuroscience 2024Quote: ... The output was relayed to the back focal plane of the objective lens (HCX IRAPO L 25×, 0.95 NA W, 2.5 mm WD, Leica) through the scan lens (LSM54-1050 ...
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bioRxiv - Developmental Biology 2021Quote: Embryos of targeted injections of DshDEP+ into the neural fold were imaged using the Stellaris 5 confocal system with the HC APO L 10×/0.30 NA water immersion objective (Leica). The sections of these embryos were imaged using the LSM 980 confocal system with the LD LCI Plan-Apochromat 25×/0.80 NA oil immersion objective (Zeiss).
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bioRxiv - Neuroscience 2021Quote: ... Cells were imaged with a LEICA DMLFSA microscope fitted with a 63x water-immersion objective (HCX APO L 63x/0.9W U-V-I, Leica) and a LEICA TCS SP2 confocal scan head ...
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bioRxiv - Neuroscience 2021Quote: ... Live imaging was performed using a Leica SP5 confocal system equipped with an acousto-optical beam splitter and an HC Fluotar L 25X/0.95 W VISR immersion objective (Leica). Fluorescence was excited by a 405 nm laser and collected by two photomultiplier tubes set to 440-500nm (mTurquoise2 ...
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bioRxiv - Molecular Biology 2019Quote: ... Fluorescent images were obtained using a fluorescence microscope (LAS AF with DMI 6000B-AFC) with HCX PL FLUOTAR L 40×/0.60na CORR PH2 objective lens (Leica). Acquired images were pseudo colored and merged using the ImageJ software ...
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bioRxiv - Immunology 2019Quote: ... Standard confocal microscopy was performed using a Leica TCS SP2 equipped with a Leica HCX APO L 40.0×0.80 W UV water objective (Leica microsystems) at a voxel resolution of 0.36 × 0.36 × 1 µm.
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bioRxiv - Biophysics 2022Quote: ... where it was focused by a cylindrical lens into the back-focal plane of a water-dipping objective (40× 0.8 NA HCX Apo L W, Leica) and subsequently reflected by the chip of an AFM cantilever (custom-coated cantilever based on model ...
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bioRxiv - Neuroscience 2022Quote: ... Time-lapse recordings were performed using a Leica SP5 or SP8 inverted confocal microscope with a 20× long-operation objective lens (HC PL FLUOTAR, L 20x/0.40 CORR, Leica). The maximum intensity projection was generated from 10-15 Z-stack images with 10-μm intervals at each time point.
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bioRxiv - Neuroscience 2022Quote: ... mice were anaesthetized using 2.5% isoflurane and 1.5 l/min air for 5 min and impacted directly over their intact head using an electromagnetic impactor (Leica Impact One Stereotaxic impactor ...
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bioRxiv - Cell Biology 2023Quote: ... was used to acquire high-resolution images to analyse the fluorescent vesicles in the proximal tubules by using 25/1.0 NA water immersion objective (HC IRAPO L, Leica). The excitation wavelength was 1040 nm tunable laser for imaging of mCherry ...
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bioRxiv - Biophysics 2023Quote: ... fluorescence and confocal reflection mode using a water dip-in objective (HCX APO L 20x/1.00 W, Leica, Wetzlar). Organoid-generated forces are relaxed after ∼ 24 hours using 10 µM cytochalsin D (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... The light sheet is created in the tower by a cylindrical lens that is focused in the back-focal plane of a vertically mounted water-dipping objective (40× 0.8 NA HCX Apo L W, Leica). The tower was placed above the imaging dish and reflected the excitation light sheet laterally with a custom-coated cantilever (40 nm Al coating on both sides of HYDRA2R-100N-TL-20 ...
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bioRxiv - Biophysics 2023Quote: ... Stellaris 5 up-right system using either a HC APO L U-V-I 10x/0.30 NA WATER (Leica) or a HC FLUOTAR L VISIR 25X/0.95 NA WATER (Leica ...
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bioRxiv - Neuroscience 2024Quote: ... under isoflurane anesthesia (2.5%, 15.l/min) male mice were impacted over the left somatosensory cortex using an electromagnetic impactor (Leica Impact One Stereotaxic impactor ...
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bioRxiv - Developmental Biology 2023Quote: All imaging was performed on a white-light Leica TCS SP8 inverted confocal microscope using either an HC FLUOTAR L 25×/0.95 VISIR water objective (Leica) or an HC PL APO 63x/1.40 CS2 oil objective (Leica) ...
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bioRxiv - Cancer Biology 2021Quote: ... and the following primary and secondary antibodies were used: rabbit anti-p53 (Leica Biosystems, NCL-L-p53-CM5p, 1:5000), rabbit anti-Mettl3 (Abclonal ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested to prepare chromatin for immunoprecipitation using either p53 polyclonal antibodies (Leica Biosystems, Cat # NCL-L-p53-CM5p) or Mettl3 polyclonal antibodies (Abclonal ...
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bioRxiv - Genetics 2020Quote: ... The cell suspension was spread on a poly-L-lysin-coated slide before observing by Structured Illumination Microscopy (SIM) with 100X/1.4 NA STED White Objective (Leica Microsystems) and VT-iSIM system (Visitech International) ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm sections from the TMA-2 human adenocarcinoma cohort were duplex stained using a Ventana autostainer with antibodies for cytokeratin AE1/AE3 (1:250, #NCL-L-AE1/AE3, Leica detected with Roche discovery yellow chromogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Images were acquired with a Leica SP8 confocal microscope using a HCX IRAPO L 25X/0,95NA water immersion objective (#11506340, Leica microsystems). Ventricule volume is estimated by the simplified ellipsoid volume calculation formula V=0.523(width in mm)2(length in mm ...
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bioRxiv - Neuroscience 2019Quote: ... The laser beams are focused into the tissue by an HCX IRAPO L water immersion objective (25x/0.95 W CORR; Leica Microsystems). The forward-detected CARS signal is measured via a non-descanned detector ...
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bioRxiv - Pathology 2020Quote: ... Lungs biopsies were acquired with Leica SP8 confocal/two-photon microscope using a HCX IRAPO L 25X/0,95NA water immersion objective (Leica microsystems). For identification of RSV infected cells ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence was detected through the objective (Olympus LUMPLFLN 60XW, 1.0 NA, or Leica HC FLUOTAR L 25x/0.95 W VISIR) and through the oil immersion condenser (numerical aperture 1.4 ...
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bioRxiv - Neuroscience 2022Quote: Immunostained samples were acquired by confocal and 2-photon microscopy using the same Leica SP8 and a coverslip corrected HCX IRAPO L 25X/0.95NA water immersion objective (Leica Microsystems). Alexa Fluor 488 and Alexa Fluor 594 were excited with lasers emitting at 488 nm and 552 nm wavelengths respectively ...
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bioRxiv - Biophysics 2023Quote: ... around individual cells are recorded in bright-field and confocal reflection mode using a 20x water dip-in objective (HCX APO L 20x/1.00 W, Leica, Wetzlar). The acquisition time for an individual image stack is below 10 seconds ...
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bioRxiv - Cancer Biology 2023Quote: ... 5µm thick sections were cut using a Leica RM2125 RTS microtome and sectioned onto Poly-L-lysine coated slides (Leica Surgipath Apex Superior adhesive slides ...
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bioRxiv - Microbiology 2023Quote: ... islandicus REY15A grown under poor media (4.5 μl) was applied to glow-discharged lacey carbon grids and then plunge-frozen using an EM GP Plunge Freezer (Leica). The cryo-EMs were collected on a 300 keV Titan Krios with a K3 camera at 1.08 Å per pixel and a total dose of 50 e-/Å2 ...
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bioRxiv - Plant Biology 2023Quote: ... Each 3 µl of protein reaction mix was loaded into a flow-chamber and immediately observed with a confocal microscope SP5 (Leica) equipped with a 60x oil objective (NA 1.42) ...
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bioRxiv - Microbiology 2023Quote: ... a small volume (5 μL) of the stained suspension is placed on a glass slide and examined under a confocal laser scanning microscope (Leica SP-5 Confocal Laser Scanning Microscope) ...
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bioRxiv - Developmental Biology 2023Quote: Live samples were imaged using a Leica Stellaris 5 laser-scanning confocal microscope with a water HC FLUOTAR L VISIR 25X/0.95 objective and HyD S detector (Leica Microsystems). Larvae were dissected and pinned to expose ventral muscles and imaged live in ambient temperature while the larvae were maintained in ice-cold HL3.1 buffer ...
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bioRxiv - Physiology 2024Quote: ... Imaging was performed with a Leica SP8 confocal microscope with an HC APO L 20x/1,00 W objective and LAS X software (Leica Microsystems). The tissue was bath perfused with bubbled room-temperature Krebs buffer (118 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: Droplet fusion was recorded in mixtures containing 20 µM Loc1p and 0.5x mass ratio total HeLa RNA with the above-mentioned setup (HC PL Fluotar L 40x/0.6 PH2 objective on Leica DMi8) in movie mode ...
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bioRxiv - Biochemistry 2024Quote: ... Condensates were imaged by phase contrast microscopy using an HC PL Fluotar L 40x/0.6 PH2 objective on a Leica DMi8 (Leica, Germany). Experiments were performed at least three independent times.
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bioRxiv - Microbiology 2024Quote: The flagellar filament sample (4.5 μl) was applied to glow-discharged lacey carbon grids and then plunge-frozen using an EM GP2 Plunge Freezer (Leica). The cryo-EM micrographs were collected on a 300 keV Titan Krios with a K3 camera at 1.11 Å per pixel and a total dose of 50 e-/Å2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue clearing was performed using the FluoClearBABB approach24 and whole mount images were then acquired using a SP8 microscope equipped with a white light laser and a HCX APO L 20x/0.95 IMM lens (Leica Microsystems, Germany). Imaging data was analyzed on a workstation (Thinkmate ...
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bioRxiv - Immunology 2021Quote: ... Six consecutive staining cycles were performed using the following 1ry Antibody-Opal fluorophore pairings: CD4 (clone 4B12, NCL-L-CD4-368 (Leica Novocastra) – Opal 520) ...
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bioRxiv - Neuroscience 2022Quote: ... osmolarity 280-320 mOsm/l) was used both to keep tissue cold during dissection and as the bathing solution during vibratome slicing (Leica VT1200S). 0.4-1 mm-thick dorsal OB slices were taken by near-horizontal slicing ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Physiology 2021Quote: ... 20 larvae per tank were anaesthetized with MS-222 (50 mg l-1, Pharma Q) prior to feeding and directly photographed individually with a microscope (Leica M165C). The larvae were then frozen in liquid nitrogen and stored at −80°C until dry mass (DM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for blocking (1 hour, room temperature) and with a mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica) or a mouse anti-γ-SG 1:100 (NCL-L-g-SARC ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 2% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was locally anesthetized with 40– 50 μL lidocaine 0.5% and disinfected with betadine.
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bioRxiv - Neuroscience 2022Quote: ... The excitation beam was focused with a water-dipping objective lens (Nikon CFI75 16× 0.8NA or Leica HC FLUOTAR L 25x 0.95NA). The average power was 100-150 mW at the sample ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were mounted for imaging on a Leica SP8 confocal with a dipping immersion objective (Leica HCX IRAPO L, 25X, N.A. 0.95). Z-stacks were taken every 10 µm ...
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bioRxiv - Plant Biology 2020Quote: ... containing 1 g/l calcofluor white M2R and 0.5 g/l evans blue and then mounted on a TCS SP5 II CLSM (Leica Microsystems, Vienna, Austria). As emission source a 405 nm UV diode was used ...
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bioRxiv - Biochemistry 2021Quote: ... 4 μL sample were applied onto a grid held in the sample grid chamber of a Leica EM GP instrument (Leica Microsystem Inc.) cooled at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... T1 seeds from plants transformed with pSS01_35Spro:LIP1-GFP and pB7WG2_35Spro:LIP1 were harvested and selected either with 240 mg/L glufosinate ammonium solution (Basta) or screened for fluorescence on a stereomicroscope (Leica M165 FC) equipped with a GFP filter (excitation ...
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bioRxiv - Cancer Biology 2021Quote: Formalin-fixed paraffin-embedded (FFPE) human skin tissue sections from organotypic tissue was stained for MITF (NCL-L-MITF, Leica Biosystems, Nussloch, Germany), MelanA (NCL-L-MITF ...