Labshake search
Citations for Leica :
51 - 100 of 292 citations for Integrin Alpha M CD11b ITGAM Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... 300 μm coronal slices of the virus-injected area of the somatosensory cortex were made using a Leica VT1200 vibratome (Leica). After the recovery period ...
-
bioRxiv - Neuroscience 2020Quote: ... Pulled glass pipette (tip inner diameter of 5–15 µm) filled with a tracer was stereotaxically inserted in the brain using Angle One stereotaxic instrument (myNeuroLab.com; currently Leica Instruments) and a small amount of the tracer (5–300 nL ...
-
bioRxiv - Neuroscience 2020Quote: ... Spain) in 0,1 M phosphate-buffered saline (PBS) and sectioned using a vibratome (Leica VT 1000S, Leica Microsystems GmbH, Germany) on the frontal ...
-
bioRxiv - Genomics 2021Quote: ... Sections of 10μm thickness were cut from the paraffine-embedded tissue blocks and mounted onto poly-ethylene naphtholate (PEN)-membrane slides (Leica). Tissue slides were stained with the following haematoxylin and eosin (H&E ...
-
bioRxiv - Neuroscience 2023Quote: ... 12μm cryosections (DRGs) and 18-20 µm cryosections (SC, skin, E15 and P0) were obtained using a standard cryostat (Leica).
-
bioRxiv - Neuroscience 2022Quote: ... brains were rapidly removed and coronal slices (300 μm) containing the right AC were prepared in a cutting solution at 1 °C using a Vibratome (VT1200 S; Leica). The cutting solution ...
-
bioRxiv - Neuroscience 2022Quote: ... the brain was cut into 2 mm thick coronal sections in 0.15 M cacodylate buffer using a vibratome (Leica VT1200). Then ...
-
bioRxiv - Neuroscience 2022Quote: ... 0.4 μm/pixel) were captured from 100 to 300 frames through an objective lens (HCX IRAPOL 25×, NA = 1.00, Leica Microsystems) at a rate of 2.5 s per frame (i.e. ...
-
bioRxiv - Neuroscience 2023Quote: ... it was washed three times with 0.1 M PB and cut along the sagittal plane using a vibratome (Leica VT2100S). Serial parasagittal sections (50 mm thick ...
-
bioRxiv - Neuroscience 2023Quote: ... 300 µm thick coronal slices from the prefrontal and somatosensory cortices were obtained with a vibrating slicer (Microm VT1200 S, Leica) and incubated for at least 20 min at 38 °C in holding artificial cerebro-spicnal fluid (ACSF ...
-
bioRxiv - Neuroscience 2023Quote: ... for 50-μm sections, the brains were embedded in 4% agar (01059-85, Nacalai Tesque) and sliced using a vibratome (VT1200S, Leica). For immunohistochemistry ...
-
bioRxiv - Cancer Biology 2023Quote: ... 5µm thick sections were cut using a Leica RM2125 RTS microtome and sectioned onto Poly-L-lysine coated slides (Leica Surgipath Apex Superior adhesive slides ...
-
bioRxiv - Neuroscience 2023Quote: ... was cryoprotected with 30% sucrose in PBS 0.1 M for 48 h and cut afterwards in 50-µm-thick coronal sections using a sliding microtome (LEICA SM2000R, Leica). Slices were collected in 6 subseries and stored at −20°C in a cryoprotective solution (30% glycerol ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Images were taken with a digital camera (Canon EOS Rebel SL3, Canon USA) attached to a stereomicroscope (Leica M-Series). The total length of each copepod (from anterior of eye spot to fork in caudal furca ...
-
bioRxiv - Neuroscience 2023Quote: ... fitted with a CMOS camera with 3.45 μm/pixel resolution (Basler ace acA2440-75 m) and a 0.63x objective lens (Leica Planapo 0.63x). Alternating dual light illumination was generated using an OptoLED system (Cairn Research ...
-
bioRxiv - Neuroscience 2023Quote: ... acute V1 slices (400-μm thick) from P28–32 (bath-application) and P27–42 (uncaging) mice were cut with a vibratome (VT 1200, Leica) in ice-cold slicing solution containing (in mM ...
-
bioRxiv - Neuroscience 2024Quote: ... were imaged at 2 µm depth intervals with a 0.75 NA 20X objective on a Leica TCS SP8 laser scanning confocal microscope (Leica Microsystems). To quantify the percentage of co-labeling between cells expressing tdTomato ...
-
bioRxiv - Neuroscience 2021Quote: ... The brains were rapidly removed from the skull and coronal brain slices containing the hippocampal formation (350μm thick) were prepared in the ice-cold cutting solution bubbled with 95%O2/5% CO2 using Vibratome VT1200S (Leica Microsystems) and then incubated in an interface chamber in ACSF containing (in mM) ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were cut into 300μm thick (for whole-cell experiments) or 500μm thick (for field recordings) coronal sections using a vibratome (VT1000S, Leica, Nussloch, Germany). Slices were transferred to an incubation chamber filled with warmed ACSF containing (in mM) ...
-
bioRxiv - Cell Biology 2021Quote: ... Yeast slurry was transferred to the 200 µm recess of an aluminum platelet (Engineering Office M. Wohlwend) and placed in an HPM100 (Leica Microsystems) for high pressure freezing ...
-
bioRxiv - Microbiology 2021Quote: ... The cells were resuspended in 80 µl of the M solution and 5 µl were used for fluorescence microscopy observation under the Leica TCS SP8 confocal microscope (Leica, Germany). The data was analyzed using the Leica Application Suite X imaging and analysis software (Leica ...
-
bioRxiv - Systems Biology 2022Quote: High-resolution confocal images with 0.333 µm z-step were acquired using a Leica DMi8 microscope with a 93X glycerol objective and LAS X software (Leica Microsystems). The region of interest was fixed on the hippocampal subregion CA1.
-
bioRxiv - Bioengineering 2019Quote: ... the impact velocity was 6.0 m/s and the depth of cortical deformation was 2 mm and 100 ms impact duration (Impact ONE; Leica Microsystems). The skin was sutured and the animals were placed in a 37°C incubator until consciousness was regained ...
-
bioRxiv - Bioengineering 2021Quote: ... the impact velocity was 6.0 m/s and the depth of cortical deformation was 2 mm with 100 ms impact duration (Impact ONE; Leica Microsystems). After the impact ...
-
bioRxiv - Neuroscience 2021Quote: ... and 60 µm thick coronal slices containing the CA1 region of hippocampus were cut on an oscillating blade vibratome (Leica VT1000, Leica, Germany) and transferred to PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... 200 μm coronal brain slices containing nuclei of the SOC including the MNTB and VNTB were cut with a vibratome (Leica VT1200S) in cold aCSF+KA ...
-
bioRxiv - Neuroscience 2023Quote: ... The gelatin cube was sectioned coronally with 100 μm thickness (except for sagittal sections tilted 12 degrees away from the midline in Fig. 1) using a vibratome (Leica VT1200). Imaging was performed using upright confocal laser-scanning microscope (LSM880 ...
-
bioRxiv - Neuroscience 2023Quote: Twenty-μm-thick coronal sections containing the MS and the CPU were prepared with a Leica SM 2000R freezing microtome (Leica Microsystems) and stored at -20 °C in 24-well tissue culture plates containing antifreeze solution [30% ethylene glycol ...
-
bioRxiv - Neuroscience 2023Quote: ... Free floating brain sections were then prepared by sectioning these fixed brains into 40-μm-thick sections in the coronal plane with a Leica VT-1000 Vibrating microtome or Sliding Microtome SM-2000R (Leica Microsystems).
-
bioRxiv - Neuroscience 2023Quote: ... the mice were perfused intracardially with heparinized 0.1 M PBS (10 U/ml of Heparin, Ratiopharm, N68542.03; 100-125 mmHg pressure using a Leica Perfusion One system) for 5 minutes to remove the blood ...
-
bioRxiv - Neuroscience 2023Quote: ... was cryoprotected with 30% sucrose in PBS 0.1 M for 48 h and cut afterwards in 50-µm-thick coronal sections using a sliding microtome (LEICA SM2000R, Leica). Slices were collected in 6 subseries and stored at −20°C in a cryoprotective solution (30% glycerol ...
-
bioRxiv - Neuroscience 2024Quote: Mice were sacrificed by cervical dislocation and sagittal slices (250-400 μm) were prepared with an angle of 10-15° to maintain rostrocaudal striatonigral projections 82 using a vibrating microtome (Leica VT1200). Slice preparation was performed in ice-cold sucrose-based artificial cerebrospinal fluid (ASCF ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were pelleted by centrifugation at ∼800 RCF for five minutes and high-pressure frozen in the 200-µm recess of an aluminum platelet (Engineering Office M. Wohlwend 241) using an HPM100 (Leica Microsystems). The samples were then freeze substituted in 0.1% uranyl acetate ...
-
bioRxiv - Biophysics 2024Quote: ... Dissected egg chambers were high-pressure frozen in gold-coated copper Type A carriers (Engineering office M. Wohlwend) using the HPM010 (Wohlwend) or the EM Ice high-pressure freezing systems (Leica Microsystems). The egg chambers were added into the 100 µm or 200 µm cavity of the planchette and 20% Ficoll (70 kDa ...
-
bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated in anti-GRPR antibody (SP4337P, Acris Antibodies) in Bond Primary Antibody Diluent (1/100, Cat# AR9352, Leica) overnight at 4°C and anti-Ecad (1/100 ...
-
bioRxiv - Cell Biology 2019Quote: ... Animals for protein loss assay were intracardially perfused with heparinized 0.01 M PBS (10 U ml-1 of heparin, Ratiopharm; ~110 mmHg pressure using a Leica Perfusion One system) for 5–10 min at room temperature until the blood was washed out ...
-
bioRxiv - Neuroscience 2020Quote: ... Coronal brain slices containing the hippocampal formation (400 μm thick) were prepared in the ice-cold cutting solution bubbled with 95% O2/5% CO2 using Vibratome VT1200S (Leica Microsystems, Germany). The slices were transferred into an interface chamber in ACSF containing (in mM) ...
-
bioRxiv - Bioengineering 2021Quote: ... they were intracardially perfused with cold heparinized 0.1 M PBS (10 U/mL of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
-
bioRxiv - Neuroscience 2020Quote: ... frozen with liquid nitrogen and cryo-sectioned (25 μm coronal section thickness for brains and 10 μm axial section thickness for spinal cords) using a cryostat (CM1850, Leica, Wetzlar, Germany) with a microtome blade (A35 ...
-
bioRxiv - Neuroscience 2020Quote: ... Coronal 20μm-think brain sections that contained the barrel cortex were cut and collected on-slide (wS1) using a cryostat (Leica, CM3050 S). The slides were then stored at −80°C until further processing ...
-
bioRxiv - Neuroscience 2020Quote: ... The brain was thoroughly washed in 0.1 M PBS and thereafter sectioned (40 µm thickness) using a vibratome (Leica VT1000, Leica Microsystems). The sections were stored in 1x PBS at 4°C.
-
bioRxiv - Cancer Biology 2019Quote: ... before intracardial perfusion with heparinized 0.01 M PBS (10 U/ml of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
-
bioRxiv - Plant Biology 2020Quote: ... Light scattering changes in the presence of 50 μM MV were induced by applying red light (1,000 μmol m−2 s−1, Leica KL 1500 LED). Scattering changes were measured first in dark for 100 s ...
-
bioRxiv - Bioengineering 2022Quote: ... The scaffolds were then stored in 0.15 M NaCl until imaging using a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective.
-
bioRxiv - Cell Biology 2023Quote: z-stacks (0.2 to 0.4μm distance between planes) of immunolabelled cells and groups of cells were acquired using an inverted scanning confocal microscope (Leica SP5-SMD), 63x oil objective (1.4NA) ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissues were cut into 50 μm slices, images captured using Carl Zeiss Axio Scan microscope (Promenade 10, Jena, Germany) or confocal scanning microscope (DMi8, Leica, Wetzlar, Germany).
-
bioRxiv - Neuroscience 2021Quote: ... 40 μm brain slices from animals perfused with 4% paraformaldehyde (in PBS, pH-7.4) were cut with a cryostat (Leica CM 1800; Leica Canada) and used for immunohistochemistry (IHC) ...
-
bioRxiv - Cell Biology 2019Quote: ... EM grids impregnated with cells were sandwiched between a 20 μm–thick copper foil and a flat aluminum disc (Engineering Office M. Wohlwend, Sennwald, Switzerland) and frozen with an HPM 010 high-pressure freezer (Leica Microsystems, Wetzlar, Germany) as described (Tsuji et al. ...
-
bioRxiv - Pathology 2021Quote: ... was performed on 5µm-thick sections of formalin-fixed lung mounted on charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems ...
-
bioRxiv - Neuroscience 2020Quote: ... 1.0 mm deep with a controlled velocity of 5.0+0.2 m/s and a dwell time of 100 ms using a stereotaxic electromagnetic impactor (Impact one, Leica; Buffalo Grove, IL, USA) equipped with a 5.0 mm flat steel tip ...