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Citations for Leica :
301 - 350 of 1215 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were imaged at 37°C using a 20× water immersion objective (Leica C PL APO CS2 20×/0.75 IMM). FLIPPER-TR was excited at 488 nm with a tuned white light laser (WLL ...
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bioRxiv - Genetics 2023Quote: ... OCT blocks were stored at -80°C and brought up to -20°C prior to cutting (CM3050S, Leica, Wetzlar, Germany). Frozen tissue sections (14µm thickness ...
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bioRxiv - Neuroscience 2024Quote: ... The embedded brains were frozen in a bath of isopentane at −30°C and stored at −80°C until sectioning using a cryostat (Leica). Immunostainings were performed following standard protocols ...
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bioRxiv - Microbiology 2019Quote: ... equipped with a digital camera (Leica DFC450 C), or directly from the roll tube ...
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bioRxiv - Systems Biology 2020Quote: ... connected to a digital camera (Leica DFC420 C) and a computer equipped with a specific software for histomorphometric analyses (Bone 3.5 ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... Detached nodules were embedded in 5% low melting point agarose and sliced into 30-μm thick sections with a rotary microtome RM2235 (Leica Microsystems). Roots were also stained with X-Gal (Affymetrix ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in 4% low-melting agarose and coronal cortical slices were generated (300 µm) with a vibrating microtome (VTS1000 Leica Microsystems). Slices were next incubated for 60 minutes at 37°C in DMEM/F-12 imaging media without phenol red supplemented with 10% fetal bovine serum (FBS).
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bioRxiv - Cell Biology 2021Quote: ... The livers were mounted in 4% low-melting agarose in PBS and cut into 100 μm – thick sections at a vibratome (Leica VT1200S). For deep tissue imaging ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 300μm thickness were embedded into 4% low melting agarose and further cut into 50 μm slices with vibrating-blade microtome (Leica, Germany). Nuclei were counterstained by adding a small drop of ProLong™ Gold Antifade Mountant with DAPI (Invitrogen™ ...
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bioRxiv - Neuroscience 2020Quote: Brain organoids (4.5 months old) were embedded into 4% low melting agarose and 300 μm slices were cut with vibrating-blade microtome (Leica, Germany). Slices were prepared using ice-cold artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Immunology 2022Quote: ... Larvae were then immediately mounted in 1% low-melting agarose containing 40 μg/ml tricaine and live imaged overnight using confocal microscopes (Zeiss 780 NLO or Leica SP8). The fluorescence intensity over time was calculated using SUM intensity projections ...
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bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips containing the cells were placed in a low volume chamber mounted on an inverted microscope (Leica DMI3000B, Leica Microsystems, Germany) and continuously perfused with fresh solution at a rate of ∼1ml/min ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips containing the cells were placed in a low volume chamber mounted on an inverted microscope (Leica DMI3000B, Leica Microsystems, Germany) and continuously perfused with fresh solution at a rate of ∼1ml/min ...
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bioRxiv - Developmental Biology 2023Quote: ... before being embedded in 5% low-melting agarose and sectioned into 100-400 μm-thick slices using a Vibratome (Leica, VT1200). Slices were blocked and permeabilized using PBST supplemented with 5% donkey serum and 0.3% Triton X at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Sectioning of the samples was accomplished at a thickness of 100 µm using a Leica CM3050 cryostat equipped with low-profile blades (Leica, 14035838382). Subsequently ...
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bioRxiv - Plant Biology 2021Quote: ... The medium blocks containing the tissue were stored at −80 °C and were acclimatized to −20 °C in the cryomicrotome (Leica CM1850). Longitudinal sections of 20 μm thickness were mounted on poly-L-lysine-coated glass slides (Zeiss ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μm thick brain slices for fluorescence in situ hybridization were cut from fresh frozen brains at -15 °C to -25 °C using a Leica CM1850 cryostat (Leica Biosystems). Slices were mounted on Superfrost Plus microscope slides (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... the samples were freeze substituted at −90 °C for 80 h in acetone supplemented with 1% OsO4 and warmed up slowly (1°C h−1) to −60 °C in an automated freeze substitution device (AFS2; Leica Microsystems). After 8 to 12 h ...
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bioRxiv - Neuroscience 2019Quote: ... Frozen brains were sectioned transversally into 30 μm thick slices at −20°C to −25°C using a Leica CM 3050S cryostat (Leica Microsystems). Floating brain sections were washed with 1X PBS ...
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bioRxiv - Plant Biology 2020Quote: Germination rate was scored by observing radical emergence in seeds plated on vertical culture plates using a stereomicroscope (Leica M165 FC). Root growth was documented photographically on vertical culture plates containing 0.8% (w/v ...
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bioRxiv - Developmental Biology 2021Quote: ... we confirmed the presence of the transgenes by determining the corresponding fluorescent markers and subsequently examined them under a widefield fluorescence microscope LEICA M165 FC (Leica Microsystems). We then euthanized the zebrafish larvae with 250 mg/L tricaine (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2021Quote: Embryonic bat heads and embryonic E16.5 mouse heads were submerged in chilled lx PBS and imaged using a stereo microscope with brightfield illumination (Leica M165 FC) in standard anatomical views ...
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bioRxiv - Immunology 2021Quote: ... The worms were mounted on a microscope slide and visualized using stereo-fluorescence microscope (Leica M165 FC or DM4-B, Leica). The fluorescence intensity was quantified using Image J ...
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bioRxiv - Bioengineering 2021Quote: ... images were taken of a mixed population of wild-type worms on a seeded plate at 1.6x magnification on a stereomicroscope (Leica M165 FC) using a 1.3 MP CMOS camera (Thorlabs DCC1645C ...
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bioRxiv - Bioengineering 2021Quote: ... gravid day 1 adults animals were picked onto prepared palmitic acid plates and plates were imaged at 2 and 5 hours at 1.6x on a stereomicroscope (Leica M165 FC) using a 1.3 MP CMOS camera (Thorlabs DCC1645C ...
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bioRxiv - Neuroscience 2020Quote: ... we manually tracked unconstrained worms on an agar pad containing OP50 bacteria using an M156 FC stereo microscope (Leica, Wetzlar, Germany) with a 1.0X Plan Apo objective and white ...
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bioRxiv - Cell Biology 2019Quote: ... After immobilization with 2 mM levamisole aggregation was scored using a fluorescent stereo microscope (Leica M165 FC, Plan Apo 2.0× objective). For PAB-1 ...
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bioRxiv - Developmental Biology 2023Quote: ... we checked for mosaic F0 based on the fluorescence of the heart under a wide-field fluorescence microscope (LEICA M165 FC) using GFP channel ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The sample was immediately imaged using a confocal microscope (Leica TCS SP5 II) and LASAF software (Leica ...
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bioRxiv - Bioengineering 2020Quote: Collagen fiber structure was visualized using confocal reflectance microscopy (Leica TCS SP5 II) using a 488nm laser line with a 40x water objective and optical zoom of 1.75x ...
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bioRxiv - Neuroscience 2021Quote: Cells were imaged using a confocal laser scanning microscope (TCS-SP5 II, Leica). Images were acquired with a 63X objective (1.5-2x zoom ...
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bioRxiv - Immunology 2020Quote: ... and analyzed using a Leica TCS SP5 II spectral confocal microscope (Leica Microsystems). The images were obtained with a Leica 40×1.25 Oil HCX PL APO CS lens (Leica Microsystems).
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bioRxiv - Neuroscience 2022Quote: ... and examined using a Leica TCS SPE-II confocal microscope (confocal is Leica TCS SPE-II and a fluorescent is Leica DM4000 B/M LED) ...
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bioRxiv - Plant Biology 2022Quote: ... and Leica TCS SP5 II AOBS confocal 619 laser scanning microscope (Leica Microsystems). Z-series sections were acquired at a step size of 0.34μm comprising of about 40 optical sections ...
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bioRxiv - Plant Biology 2023Quote: ... equipped with a Leica TCS SP5 II confocal laser scanning device (Leica Microsystems) and a 63×/1.2 water immersion objective (HCX PL APO lambda blue 63.0 × 1.20 Water UV ...
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bioRxiv - Developmental Biology 2022Quote: ... (iii) an inverted laser scanning confocal microscope Leica TCS SP5 II (Leica Microsystems) with a 63x (NA1.3 GLYCEROL ...
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bioRxiv - Neuroscience 2019Quote: ... the foil was removed and the frozen OCT block was mounted slice-side up on a specimen stub with OCT in a precooled (specimen holder, −18°C; chamber, −18°C) cryostat (Leica CM3050 S). Once the temperature of the embedded slice had equilibrated ...
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bioRxiv - Bioengineering 2020Quote: ... and frozen to −50°C before cryosliced with a step of 10 μm at −20 °C using a modified cryotome (CM 1950; 839 Leica Microsystems, Germany). The slices were air-dried for 2 hours before staining ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in 4% low temperature gelling agarose and cut into 100-150 micrometer thick section using a vibratome (Leica VT1200 S). The Proximity Ligation Assay (PLA ...
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bioRxiv - Cancer Biology 2021Quote: Larvae fixed in 4% PFA overnight at 4°C were embedded in 4% low melting temperature agarose and transverse sections collected at 200 µm intervals using a vibrating microtome (Leica VT 1000S). Sections were blocked with 1% BSA in PBS/0.3% Triton X-100 and incubated with a 1:750 dilution of mAb414 (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... embedded in 6% low melting agarose and cut into slices of 100 μm with a vibratome Leica VT 1000S (Leica, Bensheim, Germany). The slices were washed twice in 1x PBS ...
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bioRxiv - Plant Biology 2022Quote: ... Nodule primordia and nodules were embedded in 6% low-melting agarose and sliced into 40 - 50 µm thick sections using a vibrating-blade microtome (Leica VT1000 S).
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bioRxiv - Neuroscience 2022Quote: ... Transverse brainstem slices were further obtained by serially sectioning in the rostral to caudal direction isolated hindbrains mounted in a low melting point agar block using a vibratome (Leica VS 1000, Leica Microsystems, France). A 450 µm thick slice isolating the preBötC was obtained at an axial level that was ∼300 µm more caudal to the posterior limit of the facial nucleus ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... in PBS and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 300 μm sections using a slicing solution containing (in mM) ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: 3-5 week old mice were sacrificed by Ketamine/Xylacine injection and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 220 μm sections using a slicing solution continuously oxygenated containing (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were dehydrated using an ascending ethanol series (30%-100%) at 4℃ and then critically dried using a low-speed critical point dryer (Leica EM CPD300). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... the brains were removed and sectioned into 150 µm transverse slices after embedding in 2% low-melting agarose using a vibratome VT1000 (Leica, Wetzlar, Germany). The OTCs were cultured on Millicell cell cultures inserts in 6-well plates using 1 mL of FCS-free medium consisting of DMEM/F12 supplied with 1 x B27 ...
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bioRxiv - Developmental Biology 2024Quote: ... Brains were embedded in 3% low-melting point (LMP) agarose and sectioned coronally at 100 μm using a vibratome (Leica, model VT1200). Free-floating sections were incubated in block solution (2% donkey serum ...
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bioRxiv - Developmental Biology 2021Quote: ... -120 °C (BAF 60, Leica Micro-systems, Vienna, Austria) and transferred for cryo-SEM imaging using a vacuum cryo-transfer device (VCT 100 ...