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Citations for Leica :
451 - 500 of 555 citations for Exodus 2 CCL21 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
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bioRxiv - Cell Biology 2022Quote: ... using the RNAscope™ Multiplex Fluorescent V2 Assay (Cat. No. 323270) with tissue pretreatment of 15 minutes at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Plant Biology 2023Quote: The localisations of GFP-AGP and GFP-AGPΔC expressed in transformed BY-2 cells were visualized using a confocal laser scanning microscope (TCS SP8, Leica, Wetzlar, Germany) essentially as described (Tasaki et al ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were waxed in liquid paraffin for 2 h and embedded to make paraffin blocks using a tissue embedder (EG1150, Leica, Germany). Four-micron sections were cut by paraffin slicer (RM2245 ...
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bioRxiv - Physiology 2023Quote: ... Paraffin-embedded liver sections (2 μm or 7 µm for Sirius-Red staining) were processed for IHC staining using a BOND-MAX system (Leica, Germany) as previously described 54 ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval was carried out with TRIS buffer at pH 9 for 20 mins at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems). HA and Myc tag wer detected using anti-HA (see above ...
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bioRxiv - Molecular Biology 2023Quote: ... Each z-stack acquired tissue volume (2 µm spacing) with 200 Hz line frequency using LAS X (Leica Application Suite X).
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were rotary-shadowed with 2 nm of platinum and 6 nm of carbon using a high vacuum sputter coater (Leica Microsystems). The resultant platinum-replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Plant Biology 2023Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Cell Biology 2023Quote: Isolated islets were kept in culture overnight and frozen with a Leica EMpact 2 or EM ICE high-pressure freezer (Leica Microsystems). They were kept in liquid nitrogen until freeze substitution ...
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bioRxiv - Plant Biology 2024Quote: ... and Antirrhinum majus stained with SR2200 and TO-PRO-3 iodide or DAPI was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized using vaporized isoflurane (5% for induction, 1.5% for maintenance) and placed in a stereotactic frame (Leica, Angle 2). Opthalmic ointment was applied and rectal temperature and respiration rate were monitored throughout surgery ...
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bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
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bioRxiv - Cell Biology 2019Quote: The gastrocnemius and quadriceps muscles were isolated from mouse hind limbs and dehydrated in a 30% sucrose solution and then sectioned in a constant cold section box (Leica CM 3000, Germany) at −20 °C ...
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bioRxiv - Physiology 2021Quote: ... blocked samples were incubated for 1 hr at room temp in buffer A containing mouse anti-dystrophin IgG1 (1:20, #NCL-DYS2, Novocastra / Leica, Buffalo Grove, IL). The sections were then washed 3 times for 15 min with PBS and incubated in buffer A containing Alexa 488 goat anti-mouse IgG1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Systems Biology 2023Quote: ... the mouse brains were sectioned coronally into 40 μm-thick slices at −18 to −20 °C using a microtome (Leica Microsystems, Wetzlar, Germany). Floating brain sections (fixed in 4% PFA ...
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bioRxiv - Cell Biology 2023Quote: Fresh mouse kidneys were embedded in low-melting agarose and cut into 300 μm slices using a vibratome (LEICA VT1200 S, Mannheim, Germany). Slices were maintained in carbogenated Krebs-Henseleit-Buffer.14 AKS were incubated with 5 μM propidium iodide for 5 min to label cells damaged by cutting.
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bioRxiv - Cancer Biology 2024Quote: Multiplexed immunofluorescence imaging: Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
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bioRxiv - Cancer Biology 2021Quote: Unstained sections from the study cases were heated for 30 minutes in Bond™ Epitope Retrieval Solution 2 (No. AR9640; Leica Biosystems) using Leica BOND-MAXTM (Leica Microsystems) ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was blocked in 2% agar and 200 µm thick coronal sections were cut using a vibratome (Leica VT 1000 S). The slices were collected on gelatin-coated glass slides and stained with solution D and E before being dehydrated through graded alcohols and mounted using Permount (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... GFP fluorescence resulting from LanB2:GFP expression in Dilp2 neurons was imaged with a Leica TCS SP8 MP 2-photon microscope (Leica, Wetzlar, Germany).
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bioRxiv - Neuroscience 2021Quote: ... Brains were isolated and acute horizontal slices (310 μm thick) were prepared using a vibratome (5000mz-2, Campden Instruments Ltd.; or VT1200S, Leica Biosystems). Slices recovered for 30 min in ∼37°C oxygenated Ringer’s solution that was identical to the dissection solution except with lower Mg2+ concentrations (1 mM MgSO4 ...
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bioRxiv - Biophysics 2019Quote: ... Then a bulk of muscle was washed 2 to 3 times in cold PBS and fixed on the specimen holder of VT1200S vibrating blade microtome (Leica Biosystems, US). The buffer tray of microtome was ice bathed and filled with cold PBS while slicing ...
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bioRxiv - Cancer Biology 2019Quote: ... homogeneous and mixed spheroids were generated and imaged repeatedly over time (2-3 times per week) using brightfield microscopy (4x objective; 0.3 NA, Leica DM IRBE, Hamamatsu). Spheroid volumes were calculated using SpheroidSizer58.
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bioRxiv - Cell Biology 2021Quote: ... ∼30 round chondrocytes were randomly examined in each slice preparation to select the Ca2+ fluctuation-positive cells generating spontaneous events (>0.025 in Fura-2 ratio) using commercial software (Leica Application Suite X), and recording traces from the positive cells were then analyzed using Fiji/ImageJ software (US ...
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bioRxiv - Genetics 2022Quote: ... Tissue was then frozen at -20 °C for at least 2 hrs and sectioned (50–100 μm sections) on a cryostat (Leica CM3050 S) and mounted on glass slides (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Histological analysis of the 2 µm paraffin-embedded tissue sections was performed with the Bond Polymer Refine Detection Kit (Leica, Wetzlar, Germany) on a BondMax system (Leica) ...
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bioRxiv - Plant Biology 2020Quote: ... Light scattering changes in the presence of 50 μM MV were induced by applying red light (1,000 μmol m−2 s−1, Leica KL 1500 LED). Scattering changes were measured first in dark for 100 s ...
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bioRxiv - Neuroscience 2021Quote: ... the cochlea was glued on a metal block with Loctite 401 and cut to 2 halves by a vibratome (VT1200S, Leica, Wetzlar, Germany) with FREQ index at 7 ...
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bioRxiv - Molecular Biology 2020Quote: ... the number of colonies exhibiting correct fluorescence were counted (EGFP+ for 2-insert assembly, EGFP+/mCherry+ for 6-insert assembly) using a fluorescence stereoscope (Leica M165 FC). Accuracy was calculated as the percentage of colonies exhibiting correct fluorescence ...
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bioRxiv - Developmental Biology 2020Quote: ... Finally, embryos were cleared in BABB (benzyl alcohol: benzyl benzoate, 1:2) solution and vizualized under light microscope (Leica MZ6 Infinity1 stereomicroscope).
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bioRxiv - Bioengineering 2022Quote: Following embedding within 2% agarose and fixation overnight samples were dehydrated and embedded in paraffin using an automatic tissue processor (ASP300; Leica, London, UK). All samples were sectioned at a thickness of 6 μm using a rotary microtome (Leica) ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Neuroscience 2023Quote: ... brains were perfused as described above and were coronally sectioned (2 mm thick) using a vibratome (Leica Biosystems, Buffalo Grove, IL, USA). The brain slices were cleared using the Passive Clarity protocol (Yang et al. ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: 3-5 week old mice were sacrificed by Ketamine/Xylacine injection and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 220 μm sections using a slicing solution continuously oxygenated containing (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... in PBS and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 300 μm sections using a slicing solution containing (in mM) ...
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bioRxiv - Biochemistry 2023Quote: ... 3µL of the samples were dispensed onto glow discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10°C ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Biochemistry 2023Quote: 3 µl of the individual complexes were applied onto glow-discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) and vitrified in liquid ethane (−181 °C) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10 °C ...
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bioRxiv - Bioengineering 2023Quote: ... Lateral and longitudinal cross-sections of the samples were imaged after 7 days of culture in EGM-2 BulletKit medium using a Thunder imaging system (Leica Microsystems, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... the brains were removed and sectioned into 150 µm transverse slices after embedding in 2% low-melting agarose using a vibratome VT1000 (Leica, Wetzlar, Germany). The OTCs were cultured on Millicell cell cultures inserts in 6-well plates using 1 mL of FCS-free medium consisting of DMEM/F12 supplied with 1 x B27 ...
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bioRxiv - Plant Biology 2023Quote: ... Semi thin (2 μm) and ultra-thin (70 nm) sections were cut with an ultra-microtome (Leica Ultracut, Leica Microsystems, Bensheim, Germany). The ultra-thin sections were post-contrasted in a LEICA EM STAIN (Leica Microsystems ...
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bioRxiv - Neuroscience 2023Quote: ... Confocal z-stacks covering the whole depth of the neuron somata were taken with 0.38 μm step size using a 63× oil objective with 2× digital zoom magnification (Leica TCS SPE, Germany). Stacks were then processed using FIJI/ImageJ software ...
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bioRxiv - Neuroscience 2023Quote: ... Images from the same area of the CA1 region of each hippocampus were acquired using a Leica DMLB 2 microscope (Leica, Wetzlar, Germany) connected to a Leica DFC320 FireWire digital microscope camera (Leica ...