Labshake search
Citations for Leica :
1 - 50 of 1228 citations for DnaJ Hsp40 Homolog Subfamily C Member 10 DNAJC10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... frozen and cryosectioned with 10μm thickness at -10°C (Leica CM1900) with a chamber temperature of -20°C ...
-
bioRxiv - Systems Biology 2022Quote: ... MLN samples were cryosectioned (10 μm) at -20 °C on a cryostat (Leica) and mounted directly onto the 6.5 × 6.5 mm capture areas of a single Visium Spatial Gene Expression slide (10X Genomics) ...
-
bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
-
bioRxiv - Microbiology 2020Quote: ... at 4°C overnight followed by HRP labeled secondary antibody (Leica BOND) and DAB (3,3’-Diaminobenzidine ...
-
bioRxiv - Bioengineering 2020Quote: ... and frozen to −50°C before cryosliced with a step of 10 μm at −20 °C using a modified cryotome (CM 1950; 839 Leica Microsystems, Germany). The slices were air-dried for 2 hours before staining ...
-
bioRxiv - Neuroscience 2020Quote: ... and 10-µm-thick frozen sections were prepared at -20 °C (Leica CM1950, Germany). Sections were stored at -80 °C and used for immunofluorescence ...
-
bioRxiv - Neuroscience 2019Quote: Cryosections of 10 µm thickness were cut at −20°C in a cryostat (Leica Biosystems CM3050 S Research Cryostat ...
-
bioRxiv - Neuroscience 2023Quote: ... with a 10× objective for c-Fos acquisition (Leica HC PL FLUOTAR 10x/0.32) or a 63× objective for presynaptic termini acquisition (Leica HC PL FLUOTAR 63x/0.70 CORR).
-
bioRxiv - Developmental Biology 2024Quote: ... 10 µm-thick sections were cut at -20°C using a CM3050S cryostat (Leica).
-
bioRxiv - Biochemistry 2023Quote: ... Sections of 10 µm at −20 ° C were performed in cryostat (Leica CM1860UV, Leica Biosystems, Germany). The first section was stained with toluidine blue for visual inspection of the presence of islets ...
-
bioRxiv - Biochemistry 2023Quote: ... Sections of 10 µm at −20 ° C were performed in cryostat (Leica CM1860UV, Leica Biosystems, Germany). The first section was stained with toluidine blue for visual inspection of the presence of islets ...
-
bioRxiv - Bioengineering 2024Quote: ... Samples were stored at −80°C until sectioning at 10 µm on a cryostat (Leica CM1850).
-
bioRxiv - Cell Biology 2020Quote: Cells seeded at low density on glass bottom Willco dishes or cell-collagen cultures were monitored by temperature- and CO2-controlled bright-field inverse microscopy (37°C, 5%; air objectives, 10×, NA 0.20, Leica; 20×, NA 0.30, Leica; and 10×, NA 0.25, Nikon DiaPhot 300). Images were collected by CCD cameras (Sentech or Hamamatsu C8484-05G) ...
-
Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... frozen and sectioned at a thickness of 10 µm at -20 °C using a cryostat (Leica, CM1800). Sections were placed on Superfrost Microscope glass slides (Thermo Fisher Scientific) ...
-
bioRxiv - Developmental Biology 2021Quote: ... and cryo-sectioned at a thickness of 10 µm at −20°C on a cryostat (Leica, CM1850). The sections were placed on a coated glass slide (MAS-GP type A ...
-
bioRxiv - Neuroscience 2019Quote: ... Secondary antibody (polymer) incubation (10 minutes) and diaminobenzidine-based signal generation (10 minutes) were performed per manufacturer’s instructions (Leica # DS9800). Pictures were taken using SPOT Insight Gigabit camera and SPOT Imaging Software (5.1).
-
bioRxiv - Physiology 2021Quote: ... Muscle sections (10 µm) were cut from the mid belly at -20° C on a cryostat (Leica, CM1950), collected on SuperFrost Plus (VWR ...
-
bioRxiv - Physiology 2024Quote: ... Muscle sections (10 µm) were cut from the mid belly at -20°C on a cryostat (Leica, CM1950), collected on SuperFrost Plus (VWR ...
-
bioRxiv - Pathology 2022Quote: ... and incubated with a 1:200 dilution of anti-4-HNE and anti-AKR1A1 primary antibody overnight at 4°C primary antibody along with the Novolink™ Max Polymer Detection System (Leica Biosystems, Wetzlar, Hesse, Germany) and DAB substrate (3,30-diaminobenzidine ...
-
bioRxiv - Cell Biology 2020Quote: Cells seeded at low density on glass bottom Willco dishes or cell-collagen cultures were monitored by temperature- and CO2-controlled bright-field inverse microscopy (37°C, 5%; air objectives, 10×, NA 0.20, Leica; 20× ...
-
bioRxiv - Plant Biology 2023Quote: ... Grids were blotted for 2.5 s at 10 °C and 95 % humidity using a Leica EM GP plunger (Leica). Data for NCPs was acquired at the Cryo-EM facility of the Gene Centre in Munich ...
-
bioRxiv - Molecular Biology 2023Quote: ... Inhibitor-induced effects on worm viability were assessed using a stereo microscope at 10× magnification (M125 C, Leica, Germany) and the following scores ...
-
bioRxiv - Cell Biology 2022Quote: Freshly-extracted brains were frozen in isopentane at –80°C and then cut into 10 μm sections with a CM1950 Cryostat (Leica). After cutting ...
-
bioRxiv - Physiology 2021Quote: Serial OCT mounted cryostat sections (10 µm in thickness) were cut at -14°C (Leica Cryostat (Fisher Supply, Waltham, MA)) ...
-
bioRxiv - Systems Biology 2021Quote: ... The samples were stored at −80°C until 10-15 μm coronal sections were cut using a cryostat (Leica CM3050). The sections were immediately placed on the functionalized coverslips described above ...
-
bioRxiv - Biophysics 2022Quote: ... Samples were cross-sectioned into slices of 10 μm thickness by cryo-microtomy at −15°C (CM3050 S, Leica, Austria). The transversal sections were put onto microscope slides with a drop of D2O ...
-
bioRxiv - Neuroscience 2019Quote: Cryosections of 10 µm thickness were cut at −20°C in a cryostat (Leica Biosystems CM3050 S Research Cryostat, Leica Biosystems Nussloch GmbH ...
-
bioRxiv - Biochemistry 2024Quote: ... The grids were then blotted for 3.0 s at 10°C and 75% humidity and plunge-frozen in liquid ethane using an EMGP2 (Leica, Germany). Cryo-EM data were collected with a 300 kV Krios G4 transmission electron microscope (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
-
bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
-
bioRxiv - Molecular Biology 2022Quote: ... Mouse brain was embedded and frozen in optimal cut temperature (OCT) (SAKURA, USA) compound at - 80°C and 10 μm cryosection slices were prepared by using a Leica cryostat (Leica, Germany). The slices were warmed up to room temperature and fixed with 4% PFA for 5 min before used ...
-
bioRxiv - Neuroscience 2023Quote: The right hemisphere of each brain was sliced into 300μm coronal sections at −10°C using a cryostat (CM3050 S; Leica, Nuβloch, Germany). Punches of 0.75mm and 1.25mm were used to extract tissue from the frontal cortex ...
-
bioRxiv - Physiology 2024Quote: ... Brains were cryosectionned (Leica CM3050, head at - 15°C chamber -20°C), first by coarsely trimming the anterior brain until the start of the SCN ...
-
bioRxiv - Cell Biology 2021Quote: ... 10 µm thick sections covering the injured area were produced using a cryostat maintained at -20°C (Leica CM 3050s, Techno-med). To assess the integrity of mitochondrial function the sections were sequentially stained for COX and SDH activities ...
-
bioRxiv - Physiology 2022Quote: ... embedded in Tissue Tek® and after freezing at −80°C cut in 10 μm cross-sections using a Cryostat Microm HM560 (Leica Biosystems).
-
bioRxiv - Bioengineering 2022Quote: ... The samples were stored at −80°C for less than 1 week prior to sectioning (10 μm thick) using a CM1950 Cryostat (Leica, Wetzlar, Germany). Tissue sections were fixed and permeabilized in 1:1 methanol:acetone solution at −20°C ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Resin blocks were stored in −20°C until trimming into 10 µm sections with a RM 2255 microtome (Leica Biosystems, Nussloch, Germany). Sections were placed on adhesive glass slides (Menzel-Gläser Superfrost® Plus ...
-
bioRxiv - Cancer Biology 2024Quote: ... The frozen samples were stored at -20°C in preparation for tissue sectioning using a cryostat (Leica CM3050 S, 10 μm thickness).
-
bioRxiv - Synthetic Biology 2023Quote: ... The frozen samples were transferred to 1% Osmium tetroxide and 0.1% uranylacetate in acetone and freeze substituted using a Leica EM AFS2 freeze substitution processor at – 90°C with a slow warm up of 5°C per hour up to 0°C (Leica Microsystems). As osmium becomes increasingly reactive at higher temperatures we removed the samples from the AFS2 at 0°C to preserve the “crispiness” of the staining ...
-
bioRxiv - Physiology 2019Quote: ... 5 mm of the mid-belly of lateral gastrocnemius muscle was cut into 10-μm thick frozen muscle cross-sections at −20°C with a cryostat (Leica, Wetzlar, CM1850, Germany). Because of the uneven thickness of the diaphragm ...
-
bioRxiv - Genomics 2023Quote: Endometrial biopsies were fixed overnight in 10% neutral buffered formalin at 4°C and wax embedded in Surgipath Formula ‘R’ paraffin (Leica BioSystems, Wetzlar, Germany) using the Shandon Excelsior ES Tissue processor (ThermoFisher Scientific ...
-
bioRxiv - Genomics 2023Quote: ... and sliced into 13 µm slices using PTFE-coated microtome blades (Duraedge, 7223) at -10°C on a Cryostat CM1860 (Leica, 14-0491-46884). Liver sections were mounted on VWR Superfrost Plus slides (VWR ...
-
bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were snap frozen in methylbutane (−45°C ± 5°C) and sectioned at -20°C in 40 µm-thick coronal slices using a cryostat (Leica CM 1860 UV). Floating sections at the level of the MLR ...
-
bioRxiv - Bioengineering 2019Quote: ... A cryostat (Leica, −20°C) was used to cut the samples ...
-
bioRxiv - Microbiology 2023Quote: ... the samples were warmed up slowly (2 °C/h) to - 60 °C (AFS2; Leica Microsystems). After 8 to 12 h ...
-
bioRxiv - Molecular Biology 2022Quote: Muscle samples that were collected and preserved in OCT as described above were sectioned at a thickness of 10 μm in a cooled (−22°C) cryostat (Leica Biosystems; Buffalo Grove, IL, USA) and electrostatically adhered to positively charged histology slides ...
-
bioRxiv - Neuroscience 2023Quote: ... The frozen blocks (−25°C to -30°C) were transferred to a cryostat (CM1950, Leica Biosystems, USA) for sectioning ...
-
bioRxiv - Genomics 2023Quote: ... Transgenic L1-EGFP brains were sectioned on a cryostat (Leica, settings OT=-20°C, CT=-20°C) at 40µm thickness ...
-
bioRxiv - Genomics 2023Quote: ... CBA×C57BL/6 brains were sectioned on a cryostat (Leica, settings OT=-22°C, CT=-22°C) at 30µm thickness ...