Labshake search
Citations for Leica :
251 - 300 of 753 citations for Dl 4 Methoxyestradiol 13 14 15 16 17 18 13C6 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 15-µm thick sections were prepared using a CM-1950 cryostat (Leica Biosystems). Cryosections were permeabilized with 0.1 % Triton X-100 (Carl Roth ...
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bioRxiv - Genomics 2021Quote: ... The resulting 16-channel image is then spectrally deconvolved (via linear unmixing using the Leica LASX linear unmixing software) to discriminate the 8 fluorescent probes in the sample ...
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bioRxiv - Neuroscience 2019Quote: ... Brain slices (16 µm) containing the CeA were obtained on a Leica CM 3050S cryostat (Leica Biosystems, Nussloch, Germany) at −20°C and were mounted directly onto microscope slides ...
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bioRxiv - Neuroscience 2022Quote: VNOs embedded in OCT-medium were sliced in 16 µm thick sections in a cryostat apparatus (Leica CM 3050S). Slices were incubated in blocking solution containing 0.5 % Triton X-100 and 5 % normal horse serum in 0.1 M TBS for 2 h at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... 16-µm slices containing the NAc were obtained using a cryostat (CM3050 S; Leica Biosystems; Deer Park, IL, USA) at −20 °C and thaw-mounted onto Superfrost microscope slides (Fischer Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The brain tissue was then embedded in OCT medium and sliced to 14 μm thick sections on a cryostat (Leica HM 505 E). Tissue sections were collected directly onto SuperFrostPlus slides and stored at −80 deg for up to 3 months until RNA in situ hybridization for EYFP (virally driven) ...
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bioRxiv - Genetics 2020Quote: ... Serial cryosections (10 μm for embryos and 14 μm for larvae and adult zebrafish) were obtained in a Leica CM3050 S cryostat (Leica Ltd., Wetzlar, Germany) and stored at −20 °C for further use.
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bioRxiv - Neuroscience 2020Quote: 200 μm-thick hippocampal sections were isolated from 16 C57BL/6 mice of P22-28 using a vibratome (Leica, VT1200S), in the presence of ice-cold oxygenated a-CSF (125mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... A 1.5 × 1.5 mm area above A1 centered at −2.5 mm AP and 4.0 mm ML from bregma [16] was opened and the dura removed under a microscope (S6, Leica Mycrosystems). The tetrode device containing 8 NiCr electroplated tetrodes was attached to a micrometric stereotaxic arm (1760 ...
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bioRxiv - Neuroscience 2021Quote: One or two 16-channel linear electrodes with 25 micron spacing (NeuroNexus, A1×16-5mm-25-177-A16) were guided into the brain using micromanipulators (Sensapex) and a stereomicroscope (Leica). Electrical activity was amplified and digitized at 30 kHz (Spike Gadgets) ...
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bioRxiv - Neuroscience 2022Quote: ... All data was acquired as multi-channel 16-bit images using the LAS AF imaging software suite (v. 3.5; Leica Microsystems).
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bioRxiv - Plant Biology 2020Quote: ... and then cryosectioned into 15-µm-thick transverse sections using a cryostat (CM1860, Leica) in accordance with the method of Kawamoto 39 ...
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bioRxiv - Zoology 2019Quote: ... Sections of 15 μm thickness were cut with a CM 1510-3 cryostat (Leica) and collected on a SuperFrost glass slide (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... 15 μm sagittal sections were acquired using a cryostat (CM3050S; 665 Leica, Wetzlar, Germany). Tissue sections blocked 0.2% TritonX-100 ...
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bioRxiv - Neuroscience 2023Quote: ... Organoids were sectioned into 15 μm sections using a Cryostat (CM1850, Leica, Nussloch, Germany) and captured in Superfrost plus slides (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... for 15 minutes and GFP positive cells were counted using Leica DMi8 (Leica Microsystems) and the free software ImageJ Fiji (NIH Image).
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bioRxiv - Developmental Biology 2020Quote: ... Samples were embedded in the same Tissue Freezing Medium and sectioned with 16 μm thickness on a cryostat (Leica CM 3050S). Slides were incubated overnight at 4 °C and washed with PTW for rehydration ...
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bioRxiv - Neuroscience 2022Quote: Fresh-frozen brains from adult male C57BL/6J mice (8-12 weeks) were sectioned at a thickness of 16 µm using a Cryostat (Leica Biosystems). Sections were collected onto Superfrost Plus slides (Daigger Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... they were kept at 28±1°C in dark for 16 h and observed under confocal microscope (Leica microsystems, TCS, SP5). BiFC for each combination was repeated twice and appropriate negative controls were used (Kudla and Bock ...
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bioRxiv - Neuroscience 2023Quote: ... 20X (0.8 NA) 16 bit images of MCU and RGS14 immunolabeling were acquired on a Leica Thunder epifluorescence microscope (Leica DMi 8) using identical acquisition parameters for CTL and cKO and Lightning computationally clearing ...
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bioRxiv - Plant Biology 2024Quote: ... Larger stem portions were softened in boiling water with glycerin and then immersed in more concentrated solutions of polyethylene glycol 1500 (10% to 100%) (Rupp, 1964). These samples were sectioned at variable thickness (approx. 16-25 µm) using a sliding microtome (Leica SM2000R), stained with 1% Astra Blue and 1% Safranin ...
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bioRxiv - Neuroscience 2022Quote: ... Cryosections of organoids were obtained at 15 µm thickness using a cryostat (Leica CM 1950) and mounted on microscope slides (Histobond+ ...
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bioRxiv - Cell Biology 2023Quote: ... The embedded tissues were then sectioned into 15-μm slices using a CM1950 Cryostat (Leica). Sections were stained with 40 ml of 5 μM (final concentration ...
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bioRxiv - Genetics 2024Quote: ... Cryosections were cut at 15 μm thickness using a cryostat (make) (LEICA CM 1860 UV) and collected directly on charged slides (Autofrost ...
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bioRxiv - Neuroscience 2019Quote: Brains were mounted in OCT embedding media (Bright) and cut into 14 µm sections across the coronal plane using a cryostat (Leica CM 1860 UV, Ag Protect) and collected on SuperFrost Plus microscope slides (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA overnight at 4°C and afterwards cut with a vibratome (Leica Microsystems, Wetzlar Germany) in 100 μM thick sequential sagittal slices ...
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bioRxiv - Neuroscience 2023Quote: ... post-fixed in 4% PFA overnight at 4 °C and 100um thick coronal vibratome sections were made (Leica VT1000S). Images of both ipsilateral (injected ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% PFA at 4°C before 50μm coronal brain sections were cut using a vibratome (VT1200S, Leica). Slices were blocked (TBS with 0.5% Triton X-100 and 10% donkey serum ...
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bioRxiv - Neuroscience 2020Quote: Brain samples containing the hippocampus were cut serially into 16-μm-thick coronal sections using a cryostat (Leica CM 3050; Leica, Welzlar, Germany) and mounted onto glass slides ...
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bioRxiv - Neuroscience 2022Quote: ... The excitation beam was focused with a water-dipping objective lens (Nikon CFI75 16× 0.8NA or Leica HC FLUOTAR L 25x 0.95NA). The average power was 100-150 mW at the sample ...
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bioRxiv - Neuroscience 2023Quote: Each NDG was sectioned at 16 μm into serial sections using a Leica CM3050 S cryostat (Leica, Buffalo Grove, IL, United States). Sections were immediately mounted and slides were dipped in ethanol (100% ...
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bioRxiv - Neuroscience 2020Quote: ... All mouse brain tissue specimens were fixed by immersion in 4% PFA overnight at 4 °C and sectioned at 50 μm using a vibratome (Leica).
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed overnight in 4% PFA at 4 °C and sectioned at 40 μm using a vibratome (VT1000S, Leica) except for experiments involving the RNAscope kit (ACD Bio.) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then post-fixed for overnight in 4% paraformaldehyde at 4 and sectioned into 40 µm sagittal slices using a vibratome (VT1000S, Leica). Brain sections were incubated in blocking solution (0.2% Triton X-100 in PBS with 5% normal goat serum ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Brains were removed and post-fixed in 4 % PFA in PBS at 4 °C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was then extracted and saved in 4% PFA overnight at 4 °C before being resected into 70 μm slices using a VT1000s vibratome (Leica Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... then fixed overnight at 4 °C in 4% PFA and coronally sectioned at 35 μm intervals using a freezing microtome (CM3050S, Leica). Brain sections were permeabilized with a solution containing 5% bovine serum albumin and 1% Triton X-100 in PBS for 40 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the middle segment was coronally cryosectioned at 15 µm thickness on a Leica CM1860UV cryostat (Leica) and sections containing clearly defined hippocampus were transferred onto Superfrost Plus Gold microscopy slides (ThermoScientific).
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bioRxiv - Pathology 2020Quote: ... Frozen sections were cut at 15 μm on a cryostat (Leica Biosystems, Buffalo Grove, IL, USA), mounted on superfrost plus slides (Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cornea was sectioned in the central part at 15 μm with Microtome RM2145 (Leica, Germany), mounted on glass slides and stained with Eosin and Hematoxylin (Merck ...
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bioRxiv - Pathology 2023Quote: ... and blade angle slightly below 0° (Oxford) or ∼15° as measured by vibratome guide markings (Leica). As tissue sections were cut ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was sectioned coronally to 15 µm on a freezing stage microtome (Leica, Buffalo Grove, IL). Slides were mounted with duplicate sections from each brain ...
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bioRxiv - Molecular Biology 2019Quote: ... sectioned into 4 μm sections (Leica, Wetzlar, Germany), and stained with HE for microscopic assessment (Olympus ...
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bioRxiv - Developmental Biology 2019Quote: ... fixed in 4% PFA and cryo-sectioned (Leica) with a thickness of 12 μm ...
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bioRxiv - Neuroscience 2020Quote: Brain samples containing the hippocampus were cut serially into 16-μm-thick coronal sections using a cryostat (Leica CM 3050; Leica, Welzlar, Germany) and mounted onto glass slides ...
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bioRxiv - Cancer Biology 2020Quote: ... live cells images were taken every 10 minutes with fixed position for 16 hours by using Leica confocal microscope (Leica TCS SP8, Leica Microsystems Ltd, Korea), and the obtained images were analyzed by Leica LAS X program.
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bioRxiv - Bioengineering 2022Quote: ... Fixed brainstems were frozen in optimal cutting temperature compound (Sakura Finetek, Tokyo, Japan) and cut into 16 μm-thick coronal sections using a cryostat (CM3050S; Leica Microsystems, Wetzlar, Germany). Sliced sections were permeabilized and blocked with 0.1% Tween-20 and 4% donkey serum (Merck Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were frozen in Optimal Cutting Temperature (OCT) embedding media and 16 μm (for FISH) or 35 μm (for IF) coronal sections were cut with vibratome (Leica, no. CM3050 S). For FISH experiments ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA for 48 h at 4 °C and cut into 60 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in 4% paraformaldehyde overnight at 4°C, and sectioned coronally (thickness, 50–100 μm) using a vibratome (VT1200S, Leica Microsystems). Brain slices were incubated with 0.1% Triton X-100/phosphate-buffered saline for 10Cmin and blocked with Blocking-One P for 30 min ...