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Citations for Leica :
551 - 600 of 668 citations for Cow Prostaglandin E Synthase 2 PTGES2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 306C7B3 stainings were done with enzyme pretreatment (Bond Enzyme Pretreatment Kit, Leica Biosystems) at 37°C for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... blocking of endogenous peroxidase activity with Bond polymer refine detection kit (Leica: DS9800) (iii ...
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bioRxiv - Cancer Biology 2023Quote: ... This was developed by application of the Novolink DAB substrate kit (Leica Biosystems) for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... DAB refine and hematoxylin (Bond Polymer Refine Detection Kit, Leica Microsystems, Cat# DS9800) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... for 30 minutes using the Bond Polymer Refine Kit (Leica Biosystems, cat# DS9800) minus the DAB and Hematoxylin with a 1:150 dilution of OPAL520 reagent for 10 min ...
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bioRxiv - Neuroscience 2019Quote: ... The brain was snap frozen using 2-methylbutane and sliced into 40-50um coronal sections using a cryostat (Leica CM3050S, Leica Biosystems, Buffalo Grove, IL). Slices were mounted on gelatin covered microscope slides and allowed to dry ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of this mixture was back side blotted for 6 s at relative humidity 98% and temperature 19°C on a glow-discharged holey carbon grid (CF-2/1-3C, Protochips) before plunge-freezing in liquid ethane (Leica EM GP2 automatic plunger). Dose-symmetrical tilt series acquisition 51 was performed on an FEI Titan Krios electron microscope operated at 300 kV using a Gatan Quantum energy filter with a slit width of 20 eV and a K2 or K3 direct detector operated in counting mode ...
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bioRxiv - Microbiology 2019Quote: ... each for 30 min at room temperature and 100% for 2 × 1 h and 1 × 30 min) and dried in a Leica EM CPD030 (Leica Microsystems Belgium, Diegem, Belgium). The membranes were mounted on a stub and coated with 5 nm of carbon (Leica Microsystems Belgium ...
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bioRxiv - Cancer Biology 2021Quote: ... Agilent M087601-2, used at a dilution of 1/50 for 15mins at room temperature. HIER was performed on-board using Leica ER1 solution (low pH) for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035; Leica Instruments GmbH, Nuβloch, Germany). Sections were stained by hematoxylin and eosin (HE ...
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bioRxiv - Neuroscience 2020Quote: ... and resilient (n=12) mice from experiment 2 were sliced into 300 μm coronal slices on a Leica CM3050 S Research Cryostat (Leica Biosystems, Amsterdam, the Netherlands), with a chamber temperature of −12 °C and an object temperature of −10 °C ...
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bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265, Leica Microsystems Co., Ltd., Germany) and triangular glass knife ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC, Leica Microsystem GMbH, Wetzlar, Germany). When the degree of confluence reaches approximately 80 % (every 4-6 days) ...
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bioRxiv - Bioengineering 2021Quote: ... using a BOND automated stainer with a bond polymer refine detection kit (Leica Biosystems) and 3,3’-diaminobenzadine as a substrate ...
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bioRxiv - Cancer Biology 2022Quote: ... (iv) immunodetection with Revelation and counter staining Bond polymer refine detection kit (Leica: DS9800). Immunostaining was performed using a Leica Bond RX automated immunostaining device ...
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bioRxiv - Immunology 2022Quote: ... the slides were stained with the Bond Polymer Refine Detection HRP Kit (Leica Biosystem), according to the manufacturer’s instruction and counterstained with haematoxylin ...
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bioRxiv - Bioengineering 2021Quote: ... All reagents from the Bond Polymer Refine Detection Kit DC9800 (Leica, Buffalo Grove, IL) were used for IHC ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Bond polymer refine detection kit was used for DAB chromogen and hematoxylin counterstain (Leica). The tissue slides were visualized with a Vectra® Polaris™ Automated Quantitative Pathology Imaging System (Akoya Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... the signal was detected using Bond Polymer Refine Detection Kit (Leica Microsystems, catalogue #DS9800) with a diaminobenzidine reaction to detect antibody labeling and hematoxylin counterstaining.
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bioRxiv - Cell Biology 2023Quote: ... (iv) immunodetection with Revelation and counter staining Bond polymer refine detection kit (Leica: DS9800). Immunostaining was performed using a Leica Bond RX automated immunostaining device ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then processed with the Bond Polymer Refine Detection kit (#DS9800; Leica Biosystems) or the Rabbit HRP PowerVision Kit (ImmunoVisionTechnologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... before endogenous peroxidase blocking using a BOND Intense R Detection kit (#DS9263, Leica Biomarkers) for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... All images were taken no longer than 2 hrs after gavage using the wide-field feature of a LEICA DM6 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).
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bioRxiv - Microbiology 2020Quote: ... Time-lapse imaging of the samples was performed in imaging medium (RPMI1640 without phenol red containing 10% FBS and 2 µg/ml lysostaphin) on a Leica TCS SP5 confocal microscope using a 40x (Leica HC PL APO, NA=1.3) or 63x (Leica HCX PL APO ...
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Neuroscience 2020Quote: ... The slices of cochlear nucleus were cut at 220-300 μm in ice-cold sucrose solution on a vibratome (Leica VT1200S or Campden 7000smz-2). Slices were transferred into standard artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Biochemistry 2022Quote: ... and 380 nm (F380) and λem = 510 nm in a Leica DMi-8 microscope equipped with a Fura-2 filter (Leica Microsystems, Buffalo Grove, IL, USA). Cells were followed through additions of CGP (10 μM) ...
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bioRxiv - Plant Biology 2023Quote: ... mature rice pollen grains were stained with 2 % I2-KI (w/v) solution and observed under a bright field microscope (Leica DM IL LED S-80) according to Chhun et al. ...
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bioRxiv - Neuroscience 2024Quote: High-pressure freezing and freeze-substitution (HPF-FS) were performed at Harvard Medical School using the Leica EM ICE and EM AFS-2 (Leica Microsystems Inc, Buffalo Grove, IL). Whole brains were immediately frozen upon dissection ...
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bioRxiv - Cancer Biology 2019Quote: ... The reaction product was developed using the Novolink DAB substrate kit (Leica Biosystems, # RE7230-K) at RT for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... After hybridization the probes were detected with BOND Polymer Refine Red Detection kit (Leica, DS9390). Slides were counterstained with Hematoxylin for 30 seconds then dehydrated ...
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bioRxiv - Microbiology 2022Quote: ... Detection with DAB chromogen was completed using the Bond Polymer Refine Detection kit (Leica Biosystems). Slides were finally cleared through gradient alcohol and xylene washes prior to mounting and coverslipping ...
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bioRxiv - Microbiology 2022Quote: ... Detection with DAB chromogen was completed using the Bond Polymer Refine Detection kit (Leica Biosystems). The VisUCyte anti-goat horseadish peroxidase (HRP ...
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bioRxiv - Neuroscience 2023Quote: ... Von Frey filaments (Touch Test™ Sensory Evaluator Kit of 20; #39337500; Leica Biosystems, England) were depressed against the plantar arch of the HL footpads ...
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bioRxiv - Neuroscience 2023Quote: ... sections were developed using a Bond Polymer Refine Red Detection Kit (Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 10 minutes in enzyme buffer (Bond Enzyme Pretreatment Kit, Leica Biosystems) at 37°C to assist epitope retrieval ...
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bioRxiv - Cancer Biology 2024Quote: ... IgG (0.5 µg) provided with the kit or 1 µL p53 antibody (CM5, Leica Biosystems) were used for immunoprecipitation ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were then washed 2 times for 8 minutes in DPBS containing 0.3% Triton X100 and mounted on slides (Leica Surgipath® X-tra™ Adhesive, 3800202) using Vectashield Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Developmental Biology 2019Quote: ... and reporter activities from 56 hpf to 144 hpf was performed using a Leica DMi8 combined with a CSU-X1 (Yokogawa) spinning at 10 000 rpm, 2 simultaneous cameras (TuCam Flash4.0, Hamamatsu) and a water immersion objective (Leica 20X, N.A. 0.75 or Leica 40X, N.A. 1.1). 1ms exposure was used for red blood cells imaging and 20ms exposure for valve structure and reporter activity experiments ...
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bioRxiv - Immunology 2022Quote: ... Tissue sections were incubated in 3% hydrogen peroxide (Bond Polymer Refine Detection Kit DS9800, Leica Biosystems) for 15 mins to block endogenous peroxidases ...
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bioRxiv - Neuroscience 2021Quote: ... Expression of Myh11 or P-gP was detected using the Bond Polymer Refine Detection kit (Leica) and processed on automated immunostaining systems (the Bond RX Leica for P-gP and Myh11 ...
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bioRxiv - Cancer Biology 2021Quote: ... The staining process included the use of the BOND Polymer Refine Red Detection Kit (Leica Biosystems) in accordance with the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2021Quote: ... Expression of MLC1 on the sections was detected using the Bond Polymer Refine Detection Kit (Leica) with specific antibodies and an immunostaining system (Bond III ...
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bioRxiv - Microbiology 2023Quote: ... Detection with DAB chromogen was completed using the Bond Polymer Refine Detection kit (Leica Biosystems, #DS9800). Slides were finally cleared through gradient alcohol and xylene washes prior to mounting and placing cover slips ...
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bioRxiv - Neuroscience 2022Quote: ... Color development was performed with classical DAB (3,3’-diaminobenzidine) staining (Bond Polymer Refine Detection Kit, Leica Biosystems.
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bioRxiv - Immunology 2023Quote: ... platform according to manufacturer-supplied protocols using the Bond Polymer Refine Red Detection kit (Leica Biosystems) along with the RNAscope® 2.5 LS Reagent kit-Red (Advanced Cell Diagnostics ...
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bioRxiv - Cell Biology 2022Quote: ... with a Bond RX auto-stainer using the Bond Polymer Refine Kit (Leica Biosystems, Cat# DS9800) minus the peroxidase block ...
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bioRxiv - Physiology 2024Quote: ... the Leica Bond max robot was used with the BOND Polymer Refine Detection kit (Leica, DS9800). Primary antibodies were anti-Iba-1 (Wako ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were applied for 60 mins and detected with Polymer Refine Detection Kit (Leica, DS9800). For detection of P63 ...