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Citations for Leica :
601 - 650 of 777 citations for Chromosome 10 Open Reading Frame 82 C10ORF82 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... All slices were embedded in 10 % gelatin and further sectioned into slices of 60 μm thickness in ice-cold PB using a vibratome VT1000S (Leica Microsystems). After sectioning ...
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bioRxiv - Neuroscience 2021Quote: ... The frozen OCT block containing the organoid was then sectioned (10 µM thick) using a cryostat (Leica, Model CM 1950, Germany). For the immunocytochemistry staining ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Plant Biology 2021Quote: ... Samples were then mounted on aluminum stubs with carbon tape and sputter coated with 10 nm iridium (Leica EM ACE 600, Leica Microsystems) and imaged using a FEI Versa 3D scanning electron microscope (FEI ...
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bioRxiv - Neuroscience 2022Quote: ... 80% glycerol and imaged with the Leica TCS-SP2 confocal microscope using a 10x dry objective (HC PL APO CS 10×0.4, Leica, Bensheim, Germany), a 20x dry objective (HC PL APO CS 20.0×0.70 UV) ...
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bioRxiv - Bioengineering 2022Quote: ... The gels were subsequently incubated with EM containing 10 μM forskolin (Wako) with DMSO or Inh-172 for 24 h before imaging with a Leica DMi8 (Leica Microsystems) using the Leica Application Suite X (LAS-X ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were removed and placed in additional 10% formalin phosphate until sectioning into 40 µM thick sections via cryostat (CM 3050s; Leica Biosystems). Brain sections were stained with cresyl violet in order to visually confirm tetrode recording sites.
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bioRxiv - Systems Biology 2022Quote: ... followed by a blocking step for endogenous peroxidase using 0.3% H2O2/methanol and fixation with 10% neutral buffered formalin (Leica Biosystems, Germany). Slides were washed in Milli-Q water and 0.05% Tween20 in 1x Tris-Buffered Saline (TBS-T) ...
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bioRxiv - Cell Biology 2020Quote: ... CH) were carbon-coated to a thickness of 10 nm using the Leica EM ACE600 high-vacuum coating device (Leica Microsystems). The pattern of a Maxtaform H15 finder grid (Science Services ...
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bioRxiv - Physiology 2020Quote: Serial cross sections (10 μm) were cut from the TA and GA muscles using a Leica CM 3050S cryostat (Leica Microsystems). Muscle sections were fixed in cold acetone for 5 minutes at −20°C ...
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bioRxiv - Cell Biology 2021Quote: ... We used the optimized step intervals along the Z-axis to capture 3D images and the deconvolution of the acquired images was performed in the Leica Application Suite-X software using the tool blind deblur with 10 iterations (Leica Microsystems).
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bioRxiv - Cancer Biology 2021Quote: ... FFPE samples were sliced to a thickness of 10 μm and placed on a glass slide with foil (Leica, Wetzlar, Germany), stained with toluidine blue (see supplementary material ...
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bioRxiv - Biochemistry 2020Quote: ... H&E staining on 10 µm frozen section was performed using the Leica ST5020 Multistainer workstation (Leica Biosystems, Buffalo Grove, IL) according manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... samples were dehydrated in a graded ethanol series (50,70, 90, 100 and 100%) for 10 minutes each and then critical point dried (Leica CPD 300). Glass slips were adhered to a conductive carbon adhesive tab on aluminum stubs ...
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bioRxiv - Neuroscience 2020Quote: ... transverse hippocampal slices (400 μm) were prepared from male C57BL/6 mice (10-12 weeks of age) using a vibratome (Leica, VT1200S). The CA3 region was cut ...
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bioRxiv - Evolutionary Biology 2022Quote: ... using microhematocrit capillary tubes (10 μL) and measured the hematocrit rate for each mouse using a Critocaps Micro-Hematocrit Capillary Tube Reader (Leica Microsystems) (22).
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bioRxiv - Immunology 2022Quote: ... A total of 10 photomicrographs at 50x or 200x magnification per animal were randomly obtained using the microscope Leica DMI6000B (Leica microsystems). The total septal area and the total lung area were quantified using the Pro Plus 7 software (Media Cybernetics ...
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bioRxiv - Neuroscience 2022Quote: ... 10 control and 10 MK801-treated brains were cut to 10-μm thick frozen sections with a freezing microtome (Leica CM 1860 UV, Leica, Germany). The sections were stained with hematoxylin and eosin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... Mouse brain was embedded and frozen in optimal cut temperature (OCT) (SAKURA, USA) compound at - 80°C and 10 μm cryosection slices were prepared by using a Leica cryostat (Leica, Germany). The slices were warmed up to room temperature and fixed with 4% PFA for 5 min before used ...
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bioRxiv - Neuroscience 2022Quote: ... samples on SEM stubs were coated with a 10 or 15 nm platinum or gold layer using the sputter coating machine EM ACE600 (Leica Microsystems) at 30 mA current to be finally placed into the Crossbeam 540 FIB-SEM (Carl Zeiss Microscopy GmbH ...
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bioRxiv - Biochemistry 2022Quote: ... H&E staining on 10 μM frozen section was performed using the Leica ST5020 Multistainer workstation (Leica Biosystems, Buffalo Grove, IL) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Four images of each well were taken with Leica DMi8 fluorescent microscope equipped with a 10× magnification objective (Leica, Wetzlar, Germany). Overall ...
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bioRxiv - Cancer Biology 2023Quote: ... A microarray of fresh-frozen DMBA/TPA induced squamous cell carcinoma samples was cryo-sectioned with 10 μm thickness (Leica, CM3050S) and placed on the capture area of a spatial gene expression slide (10× Genomics ...
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bioRxiv - Microbiology 2023Quote: ... sectioned them at 10 µm thickness using a microtome and mounted the sections on thermoexitable POL-membranes (No. 115005191; Leica, Germany). Next ...
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bioRxiv - Neuroscience 2023Quote: ... using the same acquisition setting for all slices and at ×10 and ×63 magnification using a Leica SP8 confocal microscope (Leica, Germany). The images were analysed by a person blinded to the genotype using Qupath and ImageJ (NIH ...
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bioRxiv - Neuroscience 2023Quote: ... The paraffin-embedded brains and lumbar spinal cords were then serially sectioned at 10 μm thickness using a Leica RM2235 manual rotary microtome (Leica Biosystems) and mounted on poly-L-lysine microscope slides (ThermoFisher).
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bioRxiv - Pathology 2023Quote: ... (Supplementary table 1) were cut into 10 μm-thick sections and mounted on Leica PET-membrane slides (76463-320, Leica Microsystems). Postnatal lung tissues were considered as “healthy” after microscopic/histological analysis by a pulmonary pathologist ...
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bioRxiv - Neuroscience 2023Quote: ... compound (Sakura Finetek, CA, USA) and cryo-sectioned into 10 µm thick slices using a cryostat (CM1860; Leica Biosystems, Nussloch, Germany), The sections were mounted on glass slides ...
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bioRxiv - Developmental Biology 2022Quote: ... Processed samples were then embedded in OCT (Tissue-Tek: 4583) and transverse sections 10-14μm were taken using a cryostat (Leica: CM 3050S). EdU incorporation was visualized by using a labeling mix containing Copper Sulfate Pentahydrate (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... the mice were perfused intracardially with heparinized 0.1 M PBS (10 U/ml of Heparin, Ratiopharm, N68542.03; 100-125 mmHg pressure using a Leica Perfusion One system) for 5 minutes to remove the blood ...
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bioRxiv - Bioengineering 2023Quote: ... mice were transcardially perfused with 10% formalin and coronal slices (100 μm) containing the hippocampus were cut with a vibratome (VT1000S, Leica Microsystems).
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bioRxiv - Neuroscience 2023Quote: The right hemisphere of each brain was sliced into 300μm coronal sections at −10°C using a cryostat (CM3050 S; Leica, Nuβloch, Germany). Punches of 0.75mm and 1.25mm were used to extract tissue from the frontal cortex ...
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bioRxiv - Cancer Biology 2023Quote: ... the RNA unmasking conditions for the FFPE tissue consisted of 10-minute incubation with Proteinase K from the Bond Enzyme Pretreatment Kit at 1:1000 dilution (Leica Biosystems). HERV-K (Cat# DVF1-19321) ...
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bioRxiv - Bioengineering 2023Quote: ... was used to visualize the nano-architecture of PA hydrogel-stabilized cardiac tissue after decellularization (DECIPHER samples) and native cardiac tissue cryosections cut at 10 µm (Leica CM1950) from flash-frozen fresh young hearts embedded in OCT (Tissue-Tek ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue samples were frozen in OCT (Tissue-Tek) on dry ice and sectioned at 10 μm on a cryostat microtome (Leica CM1950). Tissue slides sectioned from the anterior of the hippocampus containing typical dentate gyrus structures were first incubated in blocking and permeation solution with 2% Triton X- 100 (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... The immunofluorescence-stained brain sections were also imaged at ×10 and ×63 magnification using a Leica SP8 confocal microscope (Leica, Germany).
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bioRxiv - Biophysics 2024Quote: ... on top of which the samples (coverslips) were mounted using 10 μl of immersion oil (Type F immersion liquid, Leica Microsystems). After assembling the coverslip ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were then embedded in OCT media and cut into 10-μm-thick sections using a CM1900 Cryostat (Leica, Wetzlar, Germany). Primary human astrocytes were fixed with 4% PFA for 20 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were harvested to prepare chromatin for immunoprecipitation using p53 polyclonal antibodies (NCL-L-p53-CM5p; Leica Biosystems). ChIPs were performed essentially as described previously (Kenzelmann Broz et al. ...
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bioRxiv - Neuroscience 2022Quote: ... slides of frontal cortex specimens stained with Clusterin antibodies were scanned on an Aperio AT2 scanner (Leica biosystems) at 20x magnification and digital slides analyzed using the QuPath platform (version 0.3.1 ...
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bioRxiv - Genetics 2020Quote: Reporter expression and fluorescent antibody-staining were imaged on an MSV269 stereoscope with a DFC365 FX camera (Leica). Freshly hatched adults (<30 minutes ...
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bioRxiv - Pathology 2020Quote: ... The primary antibodies were detected using the Bond Polymer Refine (DAB) Detection Kit (Leica Biosystems; Buffalo Grove, IL). Positive control mouse tissues for all antibodies were run in addition to negative controls performed using rat or rabbit isotype IgG.
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bioRxiv - Cancer Biology 2021Quote: The following primary antibodies were used: mouse anti-Synaptophysin (1:50, clone 27G12, Leica, NCL-L-SYNAP-299), rabbit anti-Chromogranin A (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubations were followed by signal detection using BOND Polymer Refine Detection Kit (Leica Biosystems, IL, USA) with Mayer’s hematoxylin nuclear counter stain as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibody binding is detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and Hematoxylin counterstain ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... for 30 or 40 minutes and primary antibody binding was visualized using a Polymer Refine Detection Kit (Leica). Slides were scanned with an AT2 (Leica ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with secondary antibodies and visualization were performed according to the manufacturer’s protocol (Novocastra Peroxidase Detection System, Leica). After each step ...
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bioRxiv - Immunology 2023Quote: ... Detection of the second primary antibody was performed using the Bond Polymer Red Refine Kit (DS9390, Leica Biosystems). Slides were counterstained ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody binding was detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and hematoxylin counterstain.