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Citations for Leica :
1 - 50 of 1042 citations for CU 115 CAS 2471982 20 2 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 40 s) Quantifoil Cu 300 R2/2 grid and plunge frozen in liquid ethane with a GP2 (Leica Microsystems, Wetzlar, Germany) blotted from behind ...
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bioRxiv - Biochemistry 2023Quote: ... 3µL of the samples were dispensed onto glow discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10°C ...
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bioRxiv - Biochemistry 2023Quote: 3 µl of the individual complexes were applied onto glow-discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) and vitrified in liquid ethane (−181 °C) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10 °C ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Animals were then pipetted into a type A 6mm Cu/Au carrier (Leica) and frozen in a high-pressure freezer (EM ICE ...
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bioRxiv - Microbiology 2024Quote: ... After 20 minutes of HIER with EDTA (Bond Epitope Retrieval Solution 2, Leica), tissue was blocked with Ultracruz (sc-516214 ...
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bioRxiv - Plant Biology 2023Quote: ... and images were taken every 20 min for 2 days using microscope (LEICA M205 FA). Imaging data were analyzed with ImagJ (FIJI).
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bioRxiv - Immunology 2021Quote: ... Antigen retrieval at 100 °C for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800 ...
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bioRxiv - Microbiology 2023Quote: ... antigen retrieval (20 minutes at 100°C in Leica Bond-Epitope Retrieval Solution 2 pH-9) was performed on dewaxed tissues prior to labeling with CD4 antibody (ab183685 ...
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bioRxiv - Biophysics 2022Quote: ... and frozen on a glow-discharged grid (AG150P 200 mesh Cu, Zhongjingkeyi Technology) using a high-pressure freezer (Leica HPM100, Leica Microsystems). The grid was then transferred to Leica UC7+FC7 (Leica Microsystems ...
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bioRxiv - Cancer Biology 2024Quote: ... heat-treated in ER2 (epitope retrieval solution 2) antigen retrieval buffer for 20 min at 93 °C (Leica), blocked in antibody (Ab ...
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bioRxiv - Cancer Biology 2022Quote: ... using the following conditions: antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation with anti-P53 ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed for 20 min with Bond Epitope Retrieval Solution #2 (Leica Biosystems, equivalent EDTA, pH 9.0). Primary antibody (dilution at 1 ...
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bioRxiv - Biophysics 2022Quote: ... transferred to W5 buffer, and frozen on a glow-discharged grid (AG150P 200 mesh Cu, Zhongjingkeyi Technology) using a high-pressure freezer (Leica HPM100, Leica Microsystems). The grid was then transferred to Leica UC7+FC7 (Leica Microsystems ...
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bioRxiv - Neuroscience 2022Quote: ... brains were flash frozen in 2-methylbutane and stored at −20°C until sectioning at 40μm on a cryostat (Leica Biosystems) into cryoprotectant.
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bioRxiv - Neuroscience 2023Quote: ... five µm section were cut followed by a Heat-Induced Epitope Retrieval at 100°C during 20 min in BOND Epitope Retrieval 2 (ER2) solution (Leica) with 0.1 Proteinase K before Deparaffinizing ...
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bioRxiv - Cancer Biology 2022Quote: ... with antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 1 or 2 respectively (AR9961, AR9640, Leica Biosystems). TMAs were blind-scored by multiplying staining intensity with staining coverage ...
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bioRxiv - Microbiology 2022Quote: ... Grids were post-stained for 30 min with 2% uranyl acetate (Roth) and 20 min with 3% lead citrate (Leica). TEM images were acquired using a Zeiss Leo 912 Omega (Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... frozen at -80°C for 30 minute then at -20°C for 2 hours before being sectioned using a cryostat (Leica, 50 µm thick coronal sections for immunocytochemistry) ...
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bioRxiv - Neuroscience 2024Quote: ... embedded in Tissue-Tek OCT compound (Sakura Finetek, Torrance, CA, US) and cryosectioned (20 μm of thickness; CM 1860 Cryostat; Leica, Wetzlar, Germany). Antibody staining was carried out at room temperature using standard protocols ...
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bioRxiv - Neuroscience 2021Quote: ... 20 and 20 µm thickness respectively using a cryostat (Leica). Immunohistochemistry on tissue sections will be performed according to standard procedures ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval was carried out with TRIS buffer at pH 9 for 20 mins at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems). HA and Myc tag wer detected using anti-HA (see above ...
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bioRxiv - Genetics 2022Quote: ... Tissue was then frozen at -20 °C for at least 2 hrs and sectioned (50–100 μm sections) on a cryostat (Leica CM3050 S) and mounted on glass slides (Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal brain sections (20 μm) were cut around the bregma 0 to -2 mm using a Leica CM1950 cryostat (Leica Biosystems, Germany). For P2X7R immunofluorescence labelling ...
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bioRxiv - Immunology 2024Quote: ... underwent antigen retrieval at 100°C for 20 min in Epitope Retrieval solution 2 (ER2) on a Bond Max immunostainer (Leica Biosystems, Bannockburn, IL). A rat monoclonal primary antibody to mast cell protease 1 (MCPT1;Cat# 14-5503-82 ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were first deparaffinized and then underwent antigen retrieval by incubating with EDTA-based epitope retrieval solution (BOND Epitope Retrieval Solution 2, Cas#AR9640, Leica BioSystems) for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... 20×0.75 NA (Leica Stellaris) oil-immersion objectives ...
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bioRxiv - Immunology 2023Quote: ... was equipped with a 20× objective (HC PL FLUOTAR 20×/0.50 PH2∞/0.17/D, Leica Microsystems) and with a mercury lamp (EL6000 ...
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bioRxiv - Molecular Biology 2022Quote: ... using a 20× objective (Leica, Germany). To strip off ligated sequencing probes and prepare the slides for the next sequencing cycle ...
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bioRxiv - Genomics 2023Quote: ... Transgenic L1-EGFP brains were sectioned on a cryostat (Leica, settings OT=-20°C, CT=-20°C) at 40µm thickness ...
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bioRxiv - Neuroscience 2022Quote: ... with a cryostat at 20 µm (Leica).
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bioRxiv - Neuroscience 2024Quote: ... and sectioning (20 μm, Leica CM1860 Cryostat). Sections were mounted on Superfrost Plus slides (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Sagittal cryostat sections (20 μM: Leica CM1950) were then permeabilized and blocked for 1h at RmT in IHC blocking solution ...
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bioRxiv - Cancer Biology 2024Quote: ... frozen and cryosectioned (20 µm) (Leica CM1850). Sections were observed under a fluorescent microscope (Olympus BX60 ...
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bioRxiv - Plant Biology 2021Quote: ... with a QuantEM digital camera (Roper) and a ×20 oil objective (HC PL APO ×20/0.7IMM CORR, Leica, Germany). Dual emission intensities were recorded simultaneously using a DualView with a dual CFP/YFP-ET filter set [high transmission modified Magnetron sputter-coated filter sets ET470/24m (470 indicates the emission wavelength ...
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bioRxiv - Biophysics 2023Quote: ... We cut the frozen samples in 20 µm thick serial sections at -20 C using a cryostat (Leica Biosystems), and mounted them on poly-L-lysine coated slides ...
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bioRxiv - Molecular Biology 2024Quote: ... Images were captured using a Leica DMi8 bright-field microscope equipped with a 20× objective with 0.4 NA (HC PL FL L ×20/0.40 CORR PH1, Leica Microbiosystems).
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bioRxiv - Neuroscience 2024Quote: ... with a 10 x water-immersion (HCX POL) or 20 × multi-immersion objective (HC PL APO 20 ×/0.75 Imm Corr CS2, Leica) with either water or oil as immersion medium.
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bioRxiv - Neuroscience 2022Quote: ... Tissue-Tek) at −20 °C and serial sagittal sections of 20 μm thickness were obtained using a cryostat (Leica, CM1860). Brain sections were pre-mounted on positively charged slides and washed in TBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were imaged at 37°C using a 20× water immersion objective (Leica C PL APO CS2 20×/0.75 IMM). FLIPPER-TR was excited at 488 nm with a tuned white light laser (WLL ...
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bioRxiv - Bioengineering 2023Quote: Primary Antibodies: anti-Dystrophin (Leica, NCLDYS1, 1:20), recombinant Anti-Lamin A + Lamin C antibody (Abcam ...
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bioRxiv - Immunology 2022Quote: ... coronal cryostat sections (20 µm thick: Leica, Nussloch) were thaw-mounted onto Superfrost® Plus slides ...
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bioRxiv - Immunology 2022Quote: ... and CD163 (Leica Microsystems, 10D6, 1:20 dilution). Subsequent visualization was performed using Akoya Opal fluorophores (620 ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then frozen in −20°C isopentane and cut into 20 μm-thick coronal sections on a cryostat (Leica CM1900), collected on silane-coated slides and stored at −20°C ...
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bioRxiv - Molecular Biology 2024Quote: ... were captured immediately after media replacement (T = 0) and 20 hours (T = 20) after wound generation at 10× magnification (Leica Microscope). After exporting images ...
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bioRxiv - Immunology 2022Quote: ... followed by antigen retrieval (Leica ER2 solution, 20 minutes). The protocol then proceeded as follows with Bond Wash Solution (Leica ...
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bioRxiv - Neuroscience 2020Quote: ... coronal cryostat sections (20 µm thick: Leica, Nussloch, Germany) were thaw-mounted on Superfrost®Plus slides with an embedding medium for frozen samples named OCT (Tissue-Tek)® ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μm slices were cut with a cryostat (Leica). For immunofluorescence staining ...
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bioRxiv - Bioengineering 2023Quote: ... and 20 min in 3% lead citrate (Leica, Germany). TEM images were acquired with a 200 kV TEM JEM 2100Plus (Jeol ...
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bioRxiv - Bioengineering 2023Quote: ... We used the 20× multi-immersion (NA 0.75; Leica) objective to tile-scan the whole culture stack at 512 px × 512 px resolution with a step size of Δz = 2 µm ...
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bioRxiv - Neuroscience 2024Quote: ... 20 µm thick frontal cortex sections (Leica CM1860 cryostat) were proceeded according to standard protocol ...