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Citations for Leica :
551 - 600 of 779 citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... and sliced into 13 µm slices using PTFE-coated microtome blades (Duraedge, 7223) at -10°C on a Cryostat CM1860 (Leica, 14-0491-46884). Liver sections were mounted on VWR Superfrost Plus slides (VWR ...
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bioRxiv - Systems Biology 2023Quote: ... the mouse brains were sectioned coronally into 40 μm-thick slices at −18 to −20 °C using a microtome (Leica Microsystems, Wetzlar, Germany). Floating brain sections (fixed in 4% PFA ...
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bioRxiv - Genomics 2023Quote: Endometrial biopsies were fixed overnight in 10% neutral buffered formalin at 4°C and wax embedded in Surgipath Formula ‘R’ paraffin (Leica BioSystems, Wetzlar, Germany) using the Shandon Excelsior ES Tissue processor (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
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bioRxiv - Cancer Biology 2023Quote: ... slides were baked for 2 hours at 65°C for paraffin removal and subsequently loaded onto a Leica BOND RX (Leica Biosystems, Wetzlar, Germany) for tissue rehydration in EtOH and ddH2O ...
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bioRxiv - Neuroscience 2023Quote: ... P14-P16 and P20-P21 pups of both sexes were placed in a perfused chamber at room temperature (22-25°C) in oxygenated Mg2+ containing ACSF (ACSF-1) medium under a Leica DMLFS microscope (Leica Microsystems Inc., USA). The recordings were performed using a coated nichrome electrode with an Iso-DAM8A World Precision Instruments amplifier (World Precision Instruments ...
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bioRxiv - Cell Biology 2023Quote: ... Experiments were performed at 37°C with 5% CO2 using a total internal reflection fluorescence (TIRF) microscope (Leica AM TIRF inverted microscope; Leica Microsystems, Wetzlar, Germany). Image sequences of individual particles (500 frames ...
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bioRxiv - Molecular Biology 2022Quote: The THP1 adhesion assay and the H3K9/14/18/23/27ac immunofluorescent staining was imaged with the inverted confocal laser scanning microscope TCS SP5 (Leica, Wetzlar, Germany). Analysis of the THP1 cell number and the fluorescent intensity of H3K9/14/18/23/27ac staining was performed with Fiji software (https://imagej.net/Fiji ...
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bioRxiv - Biophysics 2020Quote: ... the hind wings are dehydrated and cut into six pieces at the nanoindentation test points at the C+ScA after being embedded in paraffin using a paraffin embedding machine (Leica EG1150C, Leica Biosystems, Germany) to support the specimens in the slicing test ...
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bioRxiv - Developmental Biology 2021Quote: High-pressure frozen samples were vacuumed to 5 × 10−7 mbar at -120 °C and a fracture was conducted at the interface between the two HPF discs containing the sample (BAF 60, Leica Micro-systems, Vienna, Austria). One disc containing the exposed cryo-fixed ...
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bioRxiv - Neuroscience 2022Quote: ... containing the primary somatosensory cortex (S1) were cut in ice-cold (4 °C) NMDG ACSF using a Leica VT1200s vibratome (Leica Biosystems, Buffalo Grove, IL) and transferred to warm (33 °C ...
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bioRxiv - Genomics 2020Quote: ... individuals were lethally anaesthetized using benzocaine bath (150 mg.L−1) and a lateral photograph was taken using a digital fixed camera (Leica Microsystems; Figure 1B and C). Microbiome and host sampling consisted in gentle fish skin smears with sterile swabs ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290, Leica Microsystems GmbH, Wetzlar, Germany) with a mounted digital camera ...
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bioRxiv - Neuroscience 2020Quote: ... Sagittal sections (25 μm thick) were cut using a Leica CM1900 cryostat at a chamber temperature of −20 °C (Leica Biosystems, Buffalo Grove, IL) and mounted onto Superfrost-Plus Gold microscope slides (Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Plants were watered and left at ambient conditions (24 °C) with lid on top and imaged two days past transformation with an SP8 confocal laser scanning microscope (CLSM) (Leica Microsystems GmbH, Wetzlar, Germany) with LAS AF and SymPhoTime software (Picoquant GmbH ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of this mixture was back side blotted for 6 s at relative humidity 98% and temperature 19°C on a glow-discharged holey carbon grid (CF-2/1-3C, Protochips) before plunge-freezing in liquid ethane (Leica EM GP2 automatic plunger). Dose-symmetrical tilt series acquisition 51 was performed on an FEI Titan Krios electron microscope operated at 300 kV using a Gatan Quantum energy filter with a slit width of 20 eV and a K2 or K3 direct detector operated in counting mode ...
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bioRxiv - Cell Biology 2020Quote: ... The fresh frozen brain samples were sectioned sagittally at 14 μm thickness and the frozen brain homogenates at 20 μm thickness with a Leica CM3050 cryostat at −20°C (Leica Microsystems GmbH, Wentzler, Germany). All brain specimens were cut at approximately the same Bregma in order to clearly delineate the hippocampus ...
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bioRxiv - Cell Biology 2022Quote: ... The plates containing the pseudostratified epithelia were incubated at 37 °C with 5% CO2 in the 3i Marianis/Yokogawa Spinning-Disk Confocal microscope (Leica Microsystems, Sugar land, USA) as reported in our previous publications (53 ...
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bioRxiv - Biophysics 2022Quote: ... Confocal images and super-resolution images (Figure 3A, C, D; Figure S2A) were acquired under 100× objectives on a STED confocal microscope (Leica TCS SP8 STED 3X). Images (Figure S2C ...
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bioRxiv - Cancer Biology 2021Quote: ... After heat-induced epitope retrieval (HIER) at 100°C (BOND Epitope Retrieval Solution 1 or 2, Leica, Germany; HIER 1 AR9961, HIER 2 AR9640), endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System ...
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bioRxiv - Microbiology 2021Quote: ... pre-warmed at 37°C and the channels were imaged at x64 magnification using a DM-IRE2 confocal laser scanning microscope (Leica Microsystems Heidelberg GmbH, Germany) and the accompanying Leica Microsystems Confocal Software (version 2.61 Build 1537) ...
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bioRxiv - Biophysics 2022Quote: ... nobilis prisms from the prismatic layer of the pinna nobilis shell were fixed along the c-axis of calcite with DI water under -20°C then cut into slices with a thickness of around 30 µm using a Leica Microtome (Leica CM3050 S, Leica Microsystems Nussloch GmbH, Germany). To separate the organic shields ...
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Decoupling cell size homeostasis in diatoms from the geometrical constraints of the silica cell-wallbioRxiv - Microbiology 2023Quote: ... Vitrified samples were kept in liquid nitrogen until freeze-substitution in a freeze-substitution device at −90 °C (EM ASF2, Leica Microsystems GmbH, Wetzlar, Germany). For 48 hours ...
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Decoupling cell size homeostasis in diatoms from the geometrical constraints of the silica cell-wallbioRxiv - Microbiology 2023Quote: ... the discs were loaded into a Leica ICE high pressure freezing machine and vitrified at liquid nitrogen temperature (−192 °C) at 210 MPa (Leica Microsystems GmbH, Wetzlar, Germany). Vitrified samples were kept in liquid nitrogen until freeze-substitution in a freeze-substitution device at −90 °C (EM ASF2 ...
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Decoupling cell size homeostasis in diatoms from the geometrical constraints of the silica cell-wallbioRxiv - Microbiology 2023Quote: ... Epon-embedded samples were cured at 70 °C for 72 and then sectioned to 70 nm thin sections using Ultracut UCT ultra-microtome (Leica Microsystems GmbH, Wetzlar, Germany) equipped with an Ultra 45° diamond knife (Diatome Ltd ...
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 1 hour at 62°C in vertical slide orientation with subsequent prestaining of deparaffinization (Leica, Bond Dewax Solution, AR9222) and 35 minutes of Bond ER2 (Leica ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was incubated for 30 s at 90 % relative humidity and 16°C inside Leica EM ACE 600 (Leica EM GP2 plunge freezer), blotted for 1.2 s and vitrified in liquid ethane at liquid nitrogen temperature ...
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bioRxiv - Cell Biology 2022Quote: ... or for 4–6 s at 4°C and ~ 86% and plunge frozen using a Leica EM GP (Leica Microsystems Inc, Buffalo Grove, IL). The samples were imaged in an FEI Titan Krios electron microscope operated at 300 kV with a K2 Summit camera and energy filter (Gatan ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were flash-frozen in liquid ethane at ∼90 % humidity and 4 °C with a Leica EM GP rapid-plunging device (Leica, Buffalo Grove, IL, USA) before being transferred into liquid nitrogen for storage ...
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bioRxiv - Biochemistry 2023Quote: ... Rat hippocampus tissue punches and thin sections were prepared according to published protocols.33 Rat brain hemisphere slices 16 µm thick were cut at -16°C using a cryostat (Leica CM3050 S, Leica Biosystems GmbH, Wetzlar, Germany). Brain slices containing hippocampal areas of all three animals were deposited on the same slide ...
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bioRxiv - Cell Biology 2023Quote: ... for 4 hours and followed by Lowicryl HM20 for 24 hours with agitation and polymerized under UV exposure at −45 °C in an automated processor (AFS2, Leica Microsystem Inc., Vienna, Austria). Ultrathin sections (90 nm thickness ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were mounted with Fluorsave (Table 1S) and stored in the dark at 4°C until imaging on a Leica Stellaris5 confocal microscope (Leica Microsystems CMS GmbH, Germany). Quantification was performed as previously described42 ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were cast into molds together with liquid paraffin and cooled to form a solid paraffin block with embedded tissue (Leica EG1150 H Paraffin Embedding Station and Leica EG1150 C Cold Plate). Paraffin-embedded WAT was sliced into 5-μm sections using a ThermoScientific HM 355S Automatic Microtome and mounted on Surgipath X-tra Microscope Slides (Leica Biosystems).
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bioRxiv - Neuroscience 2021Quote: ... The brains were transferred and kept in 30% sucrose in PBS for more than 48 h at 4 °C and then sectioned using a freezing microtome (SM2010R, Leica; EF-13, Nihon Microtome Laboratory) at a thickness of 40 μm parallel to the coronal plane ...
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bioRxiv - Cell Biology 2020Quote: Cells seeded at low density on glass bottom Willco dishes or cell-collagen cultures were monitored by temperature- and CO2-controlled bright-field inverse microscopy (37°C, 5%; air objectives, 10×, NA 0.20, Leica; 20×, NA 0.30, Leica; and 10×, NA 0.25, Nikon DiaPhot 300). Images were collected by CCD cameras (Sentech or Hamamatsu C8484-05G) ...
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bioRxiv - Biophysics 2020Quote: ... the grids were lifted and held by tweezers inside the plunge freezer chamber at 90% relative humidity and 22 °C (Leica EM-GP plunger, Leica Microsystems). 1 μL drop of X5 concentrated gold nanoparticle (150 nm ...
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bioRxiv - Bioengineering 2020Quote: ... and polymerized at 60 °C for 48 h.[73] Ultrathin sections of ∼70 nm were produced in an ultra-microtome (Leica Biosystems, Buffalo Grove, IL, USA), transferred to copper grids (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The grid is immediately plunged into liquid ethane at its freezing point (−183 °C) using an automatic plunger (Leica, EM GP, Buffalo Grove, IL, USA). The vitrified specimens are analyzed using a FEI Tecnai 12 G2 TEM at 120 kV with a Gatan cryo-holder maintained at −180 °C ...
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bioRxiv - Neuroscience 2021Quote: ... The fly was positioned upside down with its head and part of the thorax on top of the glue layer under a dissection microscope (Leica M205 C, Planapo 10x objective) with forceps ...
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bioRxiv - Cell Biology 2022Quote: Plates seeded with NRVMs were incubated in a stagetop incubator at 37°C with 5% CO2 (TokaiHit, Shizuoka, Japan) on a DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Spontaneous contractions were recorded real-time under phase contrast microscopy ...
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bioRxiv - Molecular Biology 2021Quote: ... primary striatal neurons were stained with 100 nM MitoSOX™ Red reagent for 30 min at 37 °C and examined by the Automated Inverted Microscope DMI4000B (Leica Camera AG, Wetzlar, Germany).
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Oxytocin receptor activation does not mediate associative fear deficits in a Williams Syndrome modelbioRxiv - Animal Behavior and Cognition 2021Quote: ... Brains were frozen on crushed dry ice and then stored at −80 °C until sliced into 20 μm coronal sections in a cryostat (Leica Biosystems 1850, Buffalo Grove, IL) at −16 to −18 °C ...
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bioRxiv - Neuroscience 2022Quote: ... or LNPK KO hSO assembled with unlabeled hCO) were imaged under environmentally controlled conditions (37°C, 5% CO2) on a confocal microscope (Leica Stellaris, 20x objective, 2x zoom). The samples were placed in the controlled environment for 1 hour before imaging ...
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bioRxiv - Molecular Biology 2022Quote: Muscle samples that were collected and preserved in OCT as described above were sectioned at a thickness of 10 μm in a cooled (−22°C) cryostat (Leica Biosystems; Buffalo Grove, IL, USA) and electrostatically adhered to positively charged histology slides ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We sectioned brains on a coronal plane at 150 μm using a cryostat at -20°C (Leica CM3050 S, Leica Biosystems Inc., Buffalo Grove, IL) and stored the sections at -70°C until we dissected the medial prefrontal cortex ...
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bioRxiv - Cell Biology 2023Quote: ... in complete DMEM at 37°C and treated with 45 minutes of DMSO or 1mM LLOMe before immediate cryo-fixation by high pressure freezing (Leica EM ICE High Pressure Freezer), with 20% BSA in DMEM as a cryoprotectant ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then moved to 4°C for 5 min and immediately imaged using a confocal microscope (Leica SPX5 with a 63X 1.4 N.A. oil objective). Internalization of antigen was quantified by determining the percentages of cells with intracellularly-located AF488-Fab’ goat-anti-mouse IgM+G puncta in each field and by measuring the AF488 FI associated with intracellular puncta in individual cells ...
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bioRxiv - Cancer Biology 2020Quote: ... The following antibodies were diluted in Antibody Diluent (Leica) as indicated ...
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bioRxiv - Physiology 2020Quote: ... primary antibody exposure was followed by secondary antibody (Leica Biosystems) and staining using the BondTM Polymer Refine Detection kit (DS9800 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated in anti-GRPR antibody (SP4337P, Acris Antibodies) in Bond Primary Antibody Diluent (1/100, Cat# AR9352, Leica) overnight at 4°C and anti-Ecad (1/100 ...