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Citations for Leica :
1 - 50 of 180 citations for Bovine Serum Albumin BSA CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were transferred to specimen carriers and immediately frozen in the presence of 20% w/v bovine serum albumin (BSA) solution using a high-pressure freezer Leica EM ICE (Leica Microsystems, Austria). Freeze substitution was performed in the presence of 2% osmium tetroxide diluted in 100% acetone at −90 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Adrenal gland slices were allowed to recover for 15 min at 37°C in bicarbonate-buffered saline in the presence of 0.2 mM (+)-tubocurarine and then kept at room temperature in the same solution before cryofixation in external cryoprotectant (20% bovine serum albumin in bicarbonate-buffered saline) using a HPM100 HPF device (Leica). Freeze substitution was performed as previously published (Rostaing et al. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were washed with PBS containing 1% bovine serum albumin and the fluorescence (red and blue) was imaged under a fluorescence microscope (Leica). At least three fields of view were selected and analyzed using ImageJ software.
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bioRxiv - Developmental Biology 2023Quote: ... elegans animals were overlaid with 20% bovine serum albumin in M9 buffer and frozen under high-pressure using a Leica EM HPM100 system (Leica, Austria). Animals were transferred to the freeze substitution apparatus (Leica EM AFS ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were prepared by embedding hybridized embryos in gelatin-albumin and sectioning on a vibratome (Leica VT1000S) at 40 µm thickness.
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bioRxiv - Molecular Biology 2021Quote: Yeast cells in 20 % BSA were cryofixed using High Pressure Freezer (HPM101, Leica) and transferred to freeze-substitution machine (AFS2 ...
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bioRxiv - Cell Biology 2022Quote: ... intact gametocysts bathed in 3% BSA were subjected to high-pressure freezing (HPM100, Leica Microsystems), before freeze-substitution using a temperature-controlled chamber (AFS ...
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bioRxiv - Plant Biology 2022Quote: ... immersed in 20% (w/v) BSA and frozen immediately in a high-pressure freezer (Leica EM ICE ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were embedded in gelatin/ albumin with 4% of glutaraldehyde and sectioned (20 μm) on a VT1000 S vibrating blade microtome (Leica). Sections were mounted in Fluoromount Aqueous Monting Medium (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... the labelled specimens were included in a bloc of gelatin/albumin as described (Sedó-Cabezón et al., 2015) and sectioned at 40 μm in a vibrating blade microtome (Leica VT1000S). The whole epithelia or the epithelial sections were finally mounted in Mowiol medium.
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bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
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bioRxiv - Biophysics 2020Quote: ... filled with PBS-BSA (1%) and imaged with 20× (HCX PL Fluo TAR L, 0.40, dry, Leica), 40× (HCX PL Fluo TAR L ...
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bioRxiv - Cell Biology 2021Quote: ... washed with DMEM-BSA and imaged by confocal fluorescence microscopy (Leica SPX5 with a 63X 1.4 N.A. oil objective) for 60 min at 1 frame/min at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... Roots were taken and cryofixed in 20% BSA filled copper platelets (100 nm deep and 1.5 mm wide) with EM PACT1 high-pressure freezer (Leica). Subsequently ...
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bioRxiv - Neuroscience 2021Quote: The whole-mount samples were embedded in gelatin/albumin with 4% of Glutaraldehyde and sectioned at 20 μm thickness on a vibratome (Leica, VT1000 S vibrating blade microtome). The sections were mounted in Fluoromount Aqueous Mounting Medium (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then subjected to heat induced antigen retrieval and stained for anti-phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) and anti-KI-67 (CST #9027, 1/400 in PBS/2% BSA) on bond RX (LEICA). Appropriate negative and positive controls and isotype controls were included ...
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bioRxiv - Cell Biology 2020Quote: ... The loosely packed cells in BSA were high-pressure frozen in a Leica Empact 2 high-pressure freezer (Leica, Vienna, Au), and submitted to a quick freeze-substitution protocol that preserves fluorescence (35 ...
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bioRxiv - Plant Biology 2022Quote: ... immersed in 20% (w/v) BSA and frozen immediately in a high-pressure freezer (Leica EM ICE; Leica Microsystems, Vienna, Austria). Freeze substitution was carried out using a Leica EM AFS (Leica Microsystems ...
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bioRxiv - Molecular Biology 2022Quote: The samples were centrifuged and pellet was transferred to the specimen carriers which were completed with 20% BSA and immediately frozen using high pressure freezer Leica EM ICE (Leica Microsystems). Freeze substitution was performed in the presence of 2% osmium tetroxide diluted in 100% acetone at −90°C ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... Then the cells were washed in PBS and imaged in PBS-BSA using a TCS SP5 confocal microscope (Leica Microsystems, Mannheim, Germany) equipped with an HCX PL-APO 63x N.A ...
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bioRxiv - Biochemistry 2020Quote: ... Frozen bovine lenses were sectioned to 10 μm thickness equatorially or axially using a CM 3050 Cryostat (LEICA CM 3050S ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Centrifuged cells were immersed in a cryoprotectant (20% BSA in cell medium) and immediately frozen with a Leica HPM100 high-pressure freezer (Leica Microsystems, Vienna, Austria). The cells were freeze substituted in 2% OsO4 and 100% acetone using an automatic freeze substitution system Leica EM AFS2 ...
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bioRxiv - Bioengineering 2022Quote: ... Cell pellets were reconstituted in rat serum and stained with Leishman solution on frosted glass slides (Leica Biosystems). Using a light microscope (BX2 ...
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bioRxiv - Cancer Biology 2020Quote: ... and a detection kit (Bond Polymer Refine Red Detection Kit; Leica Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... Frozen bovine lenses were sectioned to 10 μm thickness equatorially or axially using a CM 3050 Cryostat (LEICA CM 3050S, Leica Microsystems Inc., Bannockburn, IL). Sections were thaw-mounted on Indium-Tin-Oxide (ITO ...
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bioRxiv - Microbiology 2022Quote: ... Cells were kept in serum- and phenol red-free MEM containing 1% glucose during the analysis (Leica Biosystems 6000B microscope). A total of 15 random snapshots (55-70 cells in total ...
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bioRxiv - Neuroscience 2019Quote: ... A Leica Refine-HRP kit (Leica Biosystems Newcastle Ltd. ...
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bioRxiv - Cancer Biology 2023Quote: ... or Bond proteolytic enzyme kit (AR9551, Leica). Thereafter ...
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bioRxiv - Physiology 2023Quote: ... or BondTM proteolytic enzyme kit (AR9551, Leica). Sections were then incubated with the indicated antibodies in BondTM primary antibody diluent (AR9352 ...
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bioRxiv - Plant Biology 2021Quote: ... using a HistoResin embedding kit (Leica, www.leica-microsystems.com), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... The Bond Refine polymer staining kit (Leica Biosystems) was used ...
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bioRxiv - Immunology 2021Quote: ... with Bond Polymer refine detection kit (Leica, DS9800). Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (Leica ...
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bioRxiv - Genomics 2021Quote: ... using the Enzyme Pretreatment Kit (AR9551; Leica Biosystems) and followed by a counterstaining with Hematoxylin (AR9551 ...
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bioRxiv - Cell Biology 2022Quote: ... a Novolink Polymer Detection Systems Kit (RE7280K; Leica Biosystems Newcastle L10 ...
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bioRxiv - Immunology 2023Quote: ... utilizing the Bond Research Detection kit (Leica Microsystems) and Opal fluorophores (Akoya Biosciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... The Bond Refine staining kit (Leica Microsystems DS9800) was used ...
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bioRxiv - Microbiology 2022Quote: ... BondTM Polymer Refine Detection visualisation kit (Leica Byosystems, Germany) was afterwards used for signal detection using DAB as chromogen and counterstaining with hematoxylin.
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bioRxiv - Immunology 2022Quote: ... The revelation system (“Bond Polymere Refine” kit, DS9800, Leica) included a secondary antibody HRP (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... the BOND Polymer Refine Detection Kit (Leica Biosystems, UK) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... and the BOND Polymer Refine Detection kit (Leica Biosystems), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Peroxide block (Bond Polymer Refine Detection Kit [DS9800], Leica Biosystems) was applied for blocking the tissue endogenous peroxidase activity for 15 mins ...
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bioRxiv - Cancer Biology 2019Quote: ... using the bond polymer refine detection kit (DS9800, Leica Biosystems). The antibody was incubated for 1 hour at a concentration of 1:100 ...
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bioRxiv - Genomics 2021Quote: ... using the Bond Polymer Refine Detection Kit (DS9800; Leica Biosystems). Sections were first applied to a peroxide blocking step for epitope retrieval using Epitope Retrieval Solution I and II (AR9640 ...
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bioRxiv - Cancer Biology 2020Quote: ... Staining was completed with BOND Polymer Refine Detection kit (Leica) including DAB chromogen and hematoxylin nuclear stain ...
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bioRxiv - Cancer Biology 2022Quote: ... using the Novolink Polymer Detection kit (Leica, Cat# RE7140-CE). Cells were counterstained with haematoxylin (Reagecon ...
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bioRxiv - Plant Biology 2022Quote: ... and infiltrated using a HistoResin embedding kit (Leica, www.leica-microsystems.com), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and the Bond Polymer Refine Detection kit (DS9800, Leica Biosystems). Positive and negative tissue controls and isotype controls were used for examining the specificity of staining and species cross-reactivity for both PHOX2B and COL1A1 in comparison with the experimental tissue slides ...
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bioRxiv - Neuroscience 2023Quote: ... Using BOND Polymer Refine Detection Kit (Leica Biosystems, IL, USA) as described (52) ...
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bioRxiv - Cell Biology 2023Quote: ... Bond Refine Detection Kit (Leica Biosystems, Newcastle Upon Tyne, UK), and Opal 6-Plex Detection Kit for Whole Slide Imaging (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Visualization used a Bond Polymer Refine Detection Kit (Leica, #DS9800) (RRID:AB_2891238 ...