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Citations for Leica :
151 - 200 of 435 citations for Borrelia burgdorferi sensu stricto B31 CRASP 2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated at 2°C for 5 days and bacmid YFP expression assessed by Leica DM IL LED Fluo microscope using a green fluorescent protein filter cube ...
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bioRxiv - Cancer Biology 2024Quote: ... unstained sections were heated for 30 minutes in BondTM Epitope Retrieval Solution 2 (No. AR9640; Leica Biosystems Newcastle LTD ...
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bioRxiv - Immunology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with phospho-DRP1 (Ser 616 ...
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bioRxiv - Plant Biology 2021Quote: ... plant tissues expressing GFP-or RFP-fused proteins were imaged with a Leica TCS SP8 confocal microscope (Leica Microsystems) using the preset settings for GFP (Ex ...
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bioRxiv - Cell Biology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with the primary antibodies.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were mounted once every 80 μm and mCherry fluorescent protein expression was detected under a microscope (Leica DMI6000). Microphotographs were taken at 2.5X magnification of each section where mCherry fluorescent signal was seen.
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bioRxiv - Cell Biology 2020Quote: ... The frozen samples were subsequently transferred into a Leica EM AFS-2 freeze substitution unit (Leica Microsystems). Over a period of five days ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.05% uranyl acetate in acetone and placed into an AFS-2 Freeze Substitution Machine (Leica Microsystems, Austria). Samples were freeze-substituted at −90°C for 72 h ...
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bioRxiv - Physiology 2021Quote: ... CFP and DsRed fluorescence signal were detected after 2 days with a CLSM (Leica AF6000 modular microsystems). All experiments were biologically repeated for three times.
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bioRxiv - Plant Biology 2022Quote: ... seedlings were mounted on a glass slide in 1/2 MS medium and imaged on SP8 (Leica). YFP was excited by the 514-nm line of the argon laser and detected between 525 and 580 nm ...
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bioRxiv - Neuroscience 2021Quote: ... or inferior colliculus were cut at 300 µm with a vibratome (VT1200S, Leica, or 7000smz-2, Campden) in warm aCSF ...
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bioRxiv - Plant Biology 2021Quote: ... the embedded samples were sectioned at 2–5 μm thickness using a rotary microtome (Leica, Wetzlar, Germany) and stained with basic fuchsin ...
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bioRxiv - Neuroscience 2020Quote: ... Antigen retrieval was done by incubation with Bond Epitope Retrieval solution 2 (AR9640, Leica Biosystems, Wetzler, Germany) for 20 minutes and then sections were then washed with Bond Wash solution 3x ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 % glutaraldehyde and 0.25 % uranyl acetate for 60 h in a FS system (AFS-2, Leica Microsystems). Larvae were embedded in an Epon-Araldite mix (hard formula ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides for CD14 and CD163 stains were retrieved for 20 minutes using Epitope Retrieval 2 (EDTA; Leica) incubated in protein block (Rodent Block M ...
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bioRxiv - Bioengineering 2022Quote: ... and counted for expression of opie-2-dsRed and 3xP3-tdTomato using a fluorescent stereoscope (Leica M165FC). The difference in total larval counts compared to total egg counts were considered to be dead during embryonic or early larval stages ...
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bioRxiv - Cell Biology 2022Quote: ... 0.05% uranyl acetate in acetone and placed in an AFS-2 Freeze-Substitution Machine (Leica Microsystems, Vienna). Samples were freeze-substituted at -90°C for 72 h ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in an FS system (AFS-2; Leica Microsystems). The embryos were embedded in an Epon-Araldite mix (EMS hard formula) ...
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bioRxiv - Immunology 2022Quote: ... Paraffin embedded kidney sections (2 µm) were cut on a Leica RM 2165 microtome (Leica, Wetzlar, Germany) and dried at 60°C for 1 h ...
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bioRxiv - Plant Biology 2023Quote: ... except for the seed compressions at 2 DPA (Fig 4) where a SP8 confocal microscope (Leica Microsystems) with a 25x long distance water-dipping objective (NA=0.95 ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in a FS system (AFS-2, Leica Microsystems). Larvae were embedded in Epon-Araldite mix (EMS hard formula) ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... 2 μm thin slides were then prepared and immunohistochemistry was performed on a Bond Rxm system (Leica) with primary antibodies against Hexokinase 1 (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... Semi thin (2 μm) and ultra-thin (70 nm) sections were cut with an ultra-microtome (Leica Ultracut ...
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bioRxiv - Cell Biology 2023Quote: ... images were captured every 2 h for 72 h by the IncuCyte Live-Cell analysis system (Leica).
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bioRxiv - Cancer Biology 2023Quote: ... Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitope Retrieval Solution 2, Leica Biosystems, cat# AR9640).
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8 ...
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bioRxiv - Cell Biology 2021Quote: Images of immunostained cells or cells expressing fluorescent tagged proteins were acquired on Confocal inverted microscope SP8-X (DMI 6000 Leica). Optical sections were acquired with a Plan Apo 63x oil immersion objective (N.A ...
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bioRxiv - Biochemistry 2020Quote: ... Protein mixture (5 μL) were deposited onto the grids and blotted for 3 seconds using an Automatic Plunge Freezer (Leica).
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bioRxiv - Bioengineering 2020Quote: ... coli constitutively expressing the Green Fluorescent protein present on biofilms was examined by laser scanning confocal microscopy (Leica TCS SP8) using oil immersion at 40X magnification ...
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bioRxiv - Plant Biology 2023Quote: ... Each 3 µl of protein reaction mix was loaded into a flow-chamber and immediately observed with a confocal microscope SP5 (Leica) equipped with a 60x oil objective (NA 1.42) ...
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bioRxiv - Plant Biology 2024Quote: ... Strains exhibiting mNeonGreen fluorescence were used to localize the RMT2-mNeonGreen fusion protein by TCS SP8 confocal laser-scanning microscopy (Leica); excitation/emission settings were 488 nm/515-530 nm using the HyD SMD hybrid detector for mNeonGreen ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
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bioRxiv - Cell Biology 2020Quote: ... 0.05% uranyl acetate in acetone and then placed in a AFS-2 freeze substitution machine (Leica Microsystems, Vienna). Samples were freeze substituted at −90°C for 72 h ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit anti-Iba1 (Wako) ...
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bioRxiv - Neuroscience 2020Quote: ... 5-6 stacks (184.58µm x 184.58µm each) from layers 2/3 of the motor cortex were obtained using high resolution confocal microscopy (Leica TCS SP8 ...
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bioRxiv - Neuroscience 2021Quote: Fluorescence images were acquired using a Leica SP5 Tandem Scanner Spectral 2-Photon scanning confocal microscope (Leica Microsystems), or Marianas Yokogawa type spinning disk confocal microscope with back-thinned EMCCD camera ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was then cut into sections of 2 μm thickness with an ultra-microtome (Leica EM UC6). Sections were placed on glass coverslips for immunohistochemistry.
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bioRxiv - Neuroscience 2019Quote: Wide-field calcium imaging of Hydra was conducted at 2 Hz using a fluorescence dissecting microscope (Leica M165) equipped with a long-pass GFP filter set (Leica filter set ET GFP M205FA/M165FC) ...
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bioRxiv - Neuroscience 2020Quote: ... Parasagittal cerebellum sections 300 μm thick were cut with a vibratome (VT1200S, Leica or 7000smz-2, Campden Instruments) in ice-cold high-sucrose ACSF ...
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bioRxiv - Neuroscience 2020Quote: Fluorescence images were acquired using a Leica SP5 Tandem Scanner Spectral 2-Photon scanning confocal microscope (Leica Microsystems), or Marianas Yokogawa type spinning disk confocal microscope with back-thinned EMCCD camera ...
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bioRxiv - Immunology 2022Quote: ... formalin-fixed paraffin-embedded tissue sections were pre-treated with the BOND Epitope Retrieval Solution 2 (Leica Biosystem) at 100°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). Embryonic and postnatal ventral midbrain tissues were fixed with 4% paraformaldehyde and dehydrated with 30% sucrose overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit mouse anti-sarcomeric α-actinin (SAA ...
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bioRxiv - Plant Biology 2021Quote: ... 2’,7’-dichlorofluorescein (DCF) fluorescence was visualized by confocal laser scanning microscopy (CLSM) (Leica TCS-SP5, Mannheim, Germany) with excitation/emission at 495 nm/525 nm.
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bioRxiv - Microbiology 2020Quote: ... 0.05 % uranyl acetate in acetone and placed in an AFS-2 freeze-substitution machine (Leica Microsystems, Wetzlar, Germany). Tissues were freeze-substituted at −90 °C for 72 h ...
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bioRxiv - Pathology 2021Quote: ... These were double contrasted using aqueous 2% uranyl acetate (Honeywell International Inc.; Morristown) and lead citrate solutions (Leica) for 10 min each ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Retrieval was accomplished using an ethylenediaminetetraacetic acid (EDTA) buffer (Bond epitope retrieval solution 2, Leica, Concord, ON, Canada), pH9 for 20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... in dry methanol for 32 hours at −90° C in an AFS 2 freeze substitution unit (Leica Biosystems). The temperature was then raised 5° C per hour to −45° C ...
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bioRxiv - Immunology 2020Quote: ... 2-photon laser scanning microscopy was performed with a LEICA SP5 inverted 5-channel confocal microscope (Leica Microsystems) equipped with a 25X water-immersion objective ...