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Citations for Leica :
1 - 50 of 672 citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... DS-Red-T (590-650 nm) and CFP-T (460-500 nm) emission filters (Leica). Images were acquired with an ORCA-Flash4.0 C13440-20C camera (Hamamatsu photonics ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... connected to a video camera (Leica DFC7000 T). Time exposure and settings for both nuclei were constant through experimental groups and images were captured at 20X magnification ...
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bioRxiv - Plant Biology 2024Quote: ... equipped with a CCD camera (DFC7000 T; Leica) and analyzed using ImageJ Fiji ...
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bioRxiv - Plant Biology 2024Quote: ... equipped with a CCD camera (DFC7000 T; Leica). The area of individual plants in the images was measured using the ImageJ Fiji software (Schindelin et al ...
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bioRxiv - Plant Biology 2024Quote: ... equipped with a CCD camera (DFC7000 T; Leica). The longest rhizoid ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
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bioRxiv - Plant Biology 2024Quote: ... equipped with a CCD camera (DFC7000 T; Leica Microsystems). For systemic phenotypic analysis ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... equipped with a cooled CCD digital camera (DFC7000 T, Leica). Double-label fluorescence in situ hybridization was performed using digoxigenin- and fluorescein-labeled RNA probes ...
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bioRxiv - Physiology 2024Quote: ... Brains were cryosectionned (Leica CM3050, head at - 15°C chamber -20°C), first by coarsely trimming the anterior brain until the start of the SCN ...
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bioRxiv - Neuroscience 2021Quote: ... with a 20× oil objective and DFC 7000-T camera (Leica Microsystems). For quantification ...
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bioRxiv - Neuroscience 2020Quote: ... and images were captured with a Leica DFC7000 T camera (Leica Microsystems).
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bioRxiv - Synthetic Biology 2023Quote: ... The frozen samples were transferred to 1% Osmium tetroxide and 0.1% uranylacetate in acetone and freeze substituted using a Leica EM AFS2 freeze substitution processor at – 90°C with a slow warm up of 5°C per hour up to 0°C (Leica Microsystems). As osmium becomes increasingly reactive at higher temperatures we removed the samples from the AFS2 at 0°C to preserve the “crispiness” of the staining ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were snap frozen in methylbutane (−45°C ± 5°C) and sectioned at -20°C in 40 µm-thick coronal slices using a cryostat (Leica CM 1860 UV). Floating sections at the level of the MLR ...
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bioRxiv - Bioengineering 2019Quote: ... A cryostat (Leica, −20°C) was used to cut the samples ...
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bioRxiv - Microbiology 2023Quote: ... the samples were warmed up slowly (2 °C/h) to - 60 °C (AFS2; Leica Microsystems). After 8 to 12 h ...
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bioRxiv - Cancer Biology 2020Quote: ... and confocal images acquired on a Leica T SC SP5 3X (Leica, Wetzlar, Germany). The number of LC3B-positive autophagosomes and autolysosomes in merged images was quantified in at least 10 cells from each treatment group.
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bioRxiv - Neuroscience 2023Quote: ... The frozen blocks (−25°C to -30°C) were transferred to a cryostat (CM1950, Leica Biosystems, USA) for sectioning ...
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bioRxiv - Genomics 2023Quote: ... Transgenic L1-EGFP brains were sectioned on a cryostat (Leica, settings OT=-20°C, CT=-20°C) at 40µm thickness ...
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bioRxiv - Genomics 2023Quote: ... CBA×C57BL/6 brains were sectioned on a cryostat (Leica, settings OT=-22°C, CT=-22°C) at 30µm thickness ...
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bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
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bioRxiv - Microbiology 2020Quote: ... or 20x (Leica C Plan, NA=0.3) objective and VisiView® software.
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bioRxiv - Biochemistry 2019Quote: ... Polymerization was visualized immediately (typically, t = ∼30 s) on a DMI6000B TIRF microscope (Leica, Wetzlar, Germany), controlled by LAS X (Leica software) ...
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bioRxiv - Cancer Biology 2020Quote: ... The TMA slides were sequentially stained with antibodies specific for CD4 (T cells, 4B12, Leica Biosystems), CD8 (T cells ...
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bioRxiv - Neuroscience 2022Quote: ... and C-X3-C motif chemokine receptor 1 (CX3CR1) and imaged with a Leica SP8 Confocal microscope (Leica Microsystems).
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bioRxiv - Neuroscience 2021Quote: ... Images were collected on a Leica DMi8 THUNDER Inverted Fluorescence Microscope with a DFC7000 T camera (Leica).
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bioRxiv - Developmental Biology 2021Quote: Images of mounted slides were captured with a DFC7000 T equipped with a M165 FC (Leica Microsystemsy) and analyzed with ImageJ (version 2.0.0).
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bioRxiv - Neuroscience 2021Quote: ... and stored at −80°C before being cut into 25 μm coronal sections in a cryostat at –20°C (CM3050S, Leica). Free-floating sections were washed with PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... and frozen in isopentane solution (−50 °C) and stored at -80 °C for later processing to 40 μm coronal sections on a freezing sliding microtome (Leica).
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bioRxiv - Neuroscience 2021Quote: ... and stored at −80 °C before being cut into 25-μm coronal sections in a cryostat at –20°C (CM3050S, Leica). Free-floating sections were washed with PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples and probes were co-denatured at 75°C for 2 min followed by incubation at 37°C overnight in a humidified chamber using ThermoBrite System (Leica). Slides were then washed for 2 min in 0.4x SSC at 75°C followed by a 30 second wash in 2x SSC at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were imaged at 37°C using a 20× water immersion objective (Leica C PL APO CS2 20×/0.75 IMM). FLIPPER-TR was excited at 488 nm with a tuned white light laser (WLL ...
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bioRxiv - Genetics 2023Quote: ... OCT blocks were stored at -80°C and brought up to -20°C prior to cutting (CM3050S, Leica, Wetzlar, Germany). Frozen tissue sections (14µm thickness ...
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bioRxiv - Neuroscience 2024Quote: ... The embedded brains were frozen in a bath of isopentane at −30°C and stored at −80°C until sectioning using a cryostat (Leica). Immunostainings were performed following standard protocols ...
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bioRxiv - Microbiology 2019Quote: ... equipped with a digital camera (Leica DFC450 C), or directly from the roll tube ...
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bioRxiv - Systems Biology 2020Quote: ... connected to a digital camera (Leica DFC420 C) and a computer equipped with a specific software for histomorphometric analyses (Bone 3.5 ...
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bioRxiv - Plant Biology 2021Quote: ... The medium blocks containing the tissue were stored at −80 °C and were acclimatized to −20 °C in the cryomicrotome (Leica CM1850). Longitudinal sections of 20 μm thickness were mounted on poly-L-lysine-coated glass slides (Zeiss ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μm thick brain slices for fluorescence in situ hybridization were cut from fresh frozen brains at -15 °C to -25 °C using a Leica CM1850 cryostat (Leica Biosystems). Slices were mounted on Superfrost Plus microscope slides (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... the samples were freeze substituted at −90 °C for 80 h in acetone supplemented with 1% OsO4 and warmed up slowly (1°C h−1) to −60 °C in an automated freeze substitution device (AFS2; Leica Microsystems). After 8 to 12 h ...
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bioRxiv - Neuroscience 2019Quote: ... Frozen brains were sectioned transversally into 30 μm thick slices at −20°C to −25°C using a Leica CM 3050S cryostat (Leica Microsystems). Floating brain sections were washed with 1X PBS ...
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bioRxiv - Microbiology 2021Quote: ... equipped with LEICA DFC7000 T CCD camera or a white light laser confocal microscope (Leica TCS SP8 X). Multicolor FISH was performed as described by Lukumbuzya et al. ...
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bioRxiv - Microbiology 2023Quote: ... equipped with LEICA DFC7000 T CCD camera or a white light laser confocal microscope (Leica TCS SP8 X). Raman microspectroscopy was applied in combination with FISH to look for the storage polymers polyphosphate (poly-P) ...
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bioRxiv - Plant Biology 2019Quote: ... the detection of protein–protein interaction by BiFC was performed using confocal microscopy (Leica SP5 CLSM). The excitation wavelength was 514 nm (argon laser ...
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bioRxiv - Bioengineering 2023Quote: ... Nonspecific protein-protein interactions were blocked with Leica PowerVision IHC/ISH Super Blocking solution (Leica PV6122) at RT ...
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bioRxiv - Neuroscience 2019Quote: ... the foil was removed and the frozen OCT block was mounted slice-side up on a specimen stub with OCT in a precooled (specimen holder, −18°C; chamber, −18°C) cryostat (Leica CM3050 S). Once the temperature of the embedded slice had equilibrated ...
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bioRxiv - Bioengineering 2020Quote: ... and frozen to −50°C before cryosliced with a step of 10 μm at −20 °C using a modified cryotome (CM 1950; 839 Leica Microsystems, Germany). The slices were air-dried for 2 hours before staining ...
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bioRxiv - Developmental Biology 2021Quote: ... -120 °C (BAF 60, Leica Micro-systems, Vienna, Austria) and transferred for cryo-SEM imaging using a vacuum cryo-transfer device (VCT 100 ...
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bioRxiv - Microbiology 2023Quote: ... The samples were cryosectioned (Leica CM 1860, −25°C) until a substantial amount of the frozen body and tentacles were exposed ...