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Citations for Leica :
501 - 550 of 725 citations for Bordetella Pertussis Toxin Frozen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... the lungs from the mice were harvested and inflated with Surgipath frozen section compound (#3801480, Leica, Buffalo Grove, IL), frozen on dry ice ...
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bioRxiv - Molecular Biology 2023Quote: ... Each specimen was frozen at - 20 °C using a Leica CM1950 cryostat (Leica CM1950; Leica Biosystems Nussloch GmbH, Germany) and trimmed until the specimen appeared on the cutting surface ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen tissues were sliced coronally into 7 - 10 μm thick sections using a cryostat machine (Leica CM3050 S, Germany), mounted on glass slides and stored at −20 °C.
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bioRxiv - Biophysics 2023Quote: ... We cut the frozen samples in 20 µm thick serial sections at -20 C using a cryostat (Leica Biosystems), and mounted them on poly-L-lysine coated slides ...
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bioRxiv - Developmental Biology 2023Quote: ... Frozen samples were stored at -80℃ prior to a coronal sectioning using a cryostat (CM3050S Leica Microsystems, 12µm sections). After equilibration at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sucrose-saturated brains were frozen on dry ice and sectioned at 30μm using a sliding microtome (Leica, Bannockburn, IL). Sections were stored in cryoprotectant solution (0.01M PBS ...
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Visualizing translating dynamics in situ at high spatial and temporal resolution in eukaryotic cellsbioRxiv - Biophysics 2023Quote: ... Before 30s pre-blotting the grid was plunge-frozen into liquid ethane using a Leica EM GP (Leica Microsystems) with 6 ∼ 8 s back-side blotting at 16 □ with the chamber at 70% humidity.
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bioRxiv - Cell Biology 2023Quote: ... The sandwiched sample was high-pressure frozen using an EM ICE high pressure freezing device (Leica Microsystems, Vienna Austria). The frozen samples were dehydrated by freeze substitution in an AFS2 freeze substitution device (Leica Microsystems ...
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bioRxiv - Neuroscience 2023Quote: ... submerged in OCT medium, flash frozen, and then sliced (50μm, parasagittal plane) using a cryostat microtome (CM 3050S, Leica).
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bioRxiv - Neuroscience 2023Quote: ... Sucrose-saturated brains were frozen on dry ice and sectioned at 30μm using a sliding microtome (Leica, Bannockburn, IL). Sections were stored in cryoprotectant solution (0.01M PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... The sandwiched sample was high-pressure frozen using an EM ICE high pressure-freezing device (Leica Microsystems, GmbH, Germany). Frozen samples were dehydrated in a temperature-controlled AFS2 Freeze substitution device (Leica Microsystems) ...
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bioRxiv - Neuroscience 2023Quote: ... The mouse brains were then cut into 20 µm thick frozen tissue sections using a cryostat (Leica, Cat# CM1950) and placed on Superfrost Plus Adhesion Microscopic glass slides (Epredia ...
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bioRxiv - Physiology 2024Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Leica Biosystems) at 95 °C for 5 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... MDM cells were subsequently plunge frozen in liquid ethane at -183°C using a EM GP plunger (Leica Microsystems), with a set chamber humidity of 90% and temperature of 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were frozen and cut into coronal sections of 25 µm thickness by using a cryostat (Leica, Weltzar, Germany). The sections were stored at −20°C until use ...
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bioRxiv - Neuroscience 2024Quote: ... brains were frozen in isopentane and cut in coronal sections of 30 μm using a cryostat (Leica Biosystems, CM1950S). Hippocampal slices were stored in antifreeze solution at -20°C until further use.
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bioRxiv - Biophysics 2021Quote: ... and incubated for 10 s prior to blotting on Whatman 1 paper for 4 s and plunge-frozen in liquid ethane using a GP2 plunge freezer (Leica) equilibrated to 10°C and 85% humidity ...
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bioRxiv - Biophysics 2020Quote: ... S cerevisiae (Sc) cells strain #2 after ~5h of starvation were high pressure frozen (HPM010, AbraFluid) and freeze substituted (EM-AFS2, Leica) with 0.1% uranyl acetate in acetone for 55h at −90°C ...
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bioRxiv - Genomics 2020Quote: Fresh frozen tissue stored at −80° Celsius was adhered to cryostat compatible metal chucks using Optimal Cutting Temperature compound (Leica) and allowed to acclimate to a −17° Celsius cryostat (Leica CM3050 S ...
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bioRxiv - Cell Biology 2022Quote: Freshly-extracted brains were frozen in isopentane at –80°C and then cut into 10 μm sections with a CM1950 Cryostat (Leica). After cutting ...
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bioRxiv - Biochemistry 2022Quote: ... excess protein was blotted away for 2.5 seconds before being plunge frozen into liquid ethane using a Leica EM GP2 plunge freezer (Leica Microsystems). Frozen grids were imaged using a Titan Krios (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... They were then transferred to 30% sucrose until they sank before being rapidly frozen and cut into 60mm sections on a freezing microtome (Leica).
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bioRxiv - Neuroscience 2021Quote: Samples were placed atop the frozen solution and the cryotubes were transferred to an AFS2 freeze substitution unit (Leica Microsystems). Samples were held at -90° for 45 hours ...
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bioRxiv - Genomics 2020Quote: Fresh frozen tissue stored at −80° Celsius was adhered to cryostat compatible metal chucks using Optimal Cutting Temperature compound (Leica) and allowed to acclimate to a −17° Celsius cryostat (Leica CM3050 S ...
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bioRxiv - Biochemistry 2019Quote: ... The grid was then plunge frozen in liquid ethane using an EM GP2 plunge freezer (Leica, 90-95% relative humidity).
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of MutS-nucleotide sample were adsorbed onto glow-discharged grids and blotted for 1 second at 80% humidity at 4°C and flash frozen in liquid ethane using an EM GP plunge freezer (Leica). All cryo-EM data was collected at the The Netherlands Center for Electron Nanoscopy (NeCEN) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were then frozen in Tissue Tek and coronal sections were cut at 30μm thickness using a cryostat (Leica CM1850).
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bioRxiv - Neuroscience 2020Quote: ... The brains were quickly frozen in embedding solution (Sakura Finetek) and cut into coronal sections using a cryostat (CM3050, Leica) at a thickness of 50 μm ...
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bioRxiv - Neuroscience 2020Quote: ... or in frozen with liquid nitrogen under a pressure of 2100 bars using a high pressure freezing system (HPM100, Leica). For HPF-frozen samples ...
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bioRxiv - Neuroscience 2021Quote: ... then equilibrated in 30% sucrose, sectioned into six series of sections (30 μM, coronal sections) on a frozen sliding microtome (SM 2000R, Leica), and stored in PBS at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Cells cultured in M9 or LS medium containing 3% sucrose were added to flat specimen carriers and frozen using a high-pressure freezing machine (Leica EM PACT ...
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bioRxiv - Microbiology 2021Quote: ... excess protein was blotted away for 2.5 seconds before being plunge frozen into liquid ethane using a Leica EM GP2 plunge freezer (Leica Microsystems). Frozen grids were imaged using a Titan Krios (Thermo Fisher ...
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bioRxiv - Microbiology 2019Quote: ... Samples were frozen in liquid nitrogen and then sectioned with a Leica Ultracut UCT7 cryo-ultramicrotome (Leica Microsystems, Wetzlar, Germany). 50 nm sections were blocked with 5% fetal bovine serum/5% normal goat serum for 30 min and subsequently incubated with primary antibody for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Grids were blotted between 2.5 and 3 sec and plunged frozen in liquid ethane using a Leica vitrification plunger (EM GP2, Leica Microsystems). For RiboRad6mix ...
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bioRxiv - Biophysics 2020Quote: ... excess protein was blotted away for 2.5 seconds before being plunge frozen into liquid ethane using a Leica EM GP2 plunge freezer (Leica Microsystems). Frozen grids for the N234A mutant were imaged in a Titan Krios (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Switzerland) previously coated with a carbon film87 and frozen with a Leica ICE high pressure freezer machine (Leica microsystems, Austria) with foetal calf serum as cryoprotectant ...
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bioRxiv - Neuroscience 2022Quote: ... brains were flash frozen in 2-methylbutane and stored at −20°C until sectioning at 40μm on a cryostat (Leica Biosystems) into cryoprotectant.
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bioRxiv - Microbiology 2022Quote: ... Dehydrated samples were frozen in Tissue-Tek OCT and cut into 15 μm cross sections with a CM1950 cryostat (Leica). Cryostat sections were incubated in permeabilization buffer (PBS containing 1% saponin ...
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bioRxiv - Immunology 2020Quote: ... excess protein was blotted away for 2.5 seconds before being plunge frozen into liquid ethane using a Leica EM GP2 plunge freezer (Leica Microsystems).
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bioRxiv - Neuroscience 2021Quote: ... Tissue was rapidly frozen using optimal cutting temperature compound (OCT) and cut into 14-µm-thick slices on a cryostat (CM 3050S, Leica). Tissue and slices were stored at -80°C.
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bioRxiv - Physiology 2021Quote: ... snap-frozen in 2-methylbutane on dry ice and sectioned into 10 μm slices using CryoJane Tape-Transfer System (Leica). Masson’s trichrome stain for cardiac fibrosis was performed according to the manufacturer’s instructions (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... excess protein was blotted away for 2.5 seconds before being plunge frozen into liquid ethane using a Leica EM GP2 plunge freezer (Leica Microsystems). Frozen grids were imaged in a Titan Krios (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... embedded in OCT, frozen and cryosectioned (coronally at 40μm, between Bregma positions 1.0mm-2.0mm) in the CM3050S cryostat (Leica Biosystems, Nussloch, DE). Brain sections were collected on adherent slides and stored at −20°C.
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bioRxiv - Biochemistry 2020Quote: ... 2.5 μl of the solution was applied on glow discharged C-flatTM grids (Protochips) and the grids were blotted and frozen with KF80 specimen plunge-freezing device (Leica) or Vitrobot Mark IV (Thermo Fisher Scientific)
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bioRxiv - Cell Biology 2020Quote: ... The sandwiched samples were high-pressure frozen instantaneously using the using the HPM-010 high pressure freezing machine (Leica Microsystems), transferred to cryo-vials (Biozym ...
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bioRxiv - Genetics 2021Quote: ... Samples were frozen in an ethanol/dry-ice bath and 10 to 14-micron thick sections were cut with a cryostat (Leica). Sections were permeabilized and blocked for 1-hour at room temperature with 0.3% Triton X-100 and 10% normal donkey serum ...
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bioRxiv - Cell Biology 2020Quote: Frozen sections (10μm) of muscle embedded in OCT of mid-belly level were made using a cryostat (Leica CM 1950) and collected on Superfrost Ultra Plus slides (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... brains were frozen in isopentane (−40°C) and sliced in serial coronal 40μm sections by using a cryostat (CM-1900, Leica, Germany). Samples were maintained in antifreeze solution before immunostaining procedure.
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bioRxiv - Cell Biology 2021Quote: ... Ten μl of these pachytene cells in agarose were placed on a flat carrier (1.2 mm of diameter and 200 μm of depth) and high-pressure frozen in the EMPACT2 high-pressure freezer (in accordince with the manufacturer’s instructions, Leica Microsystems).
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bioRxiv - Genomics 2020Quote: Fresh frozen tissue stored at −80° Celsius was adhered to cryostat compatible metal chucks using Optimal Cutting Temperature compound (Leica) and allowed to acclimate to a −17° Celsius cryostat (Leica CM3050 S ...