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Citations for Leica :
1 - 50 of 306 citations for Bicinchoninic Acid BCA Protein Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... proteins fixed in 30% ethanol/15% acetic acid and the gel piece embedded in tissue embedding media (Leica). After careful mounting on a cryo-holder ...
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bioRxiv - Cell Biology 2020Quote: ... acid alcohol (Leica) for 5 s ...
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bioRxiv - Immunology 2022Quote: ... CA) was used with the Leica RNAScope 2.5 LS Assay-RED kit and a hematoxylin counterstain (Leica Biosystems). Slides were treated in protease (Advanced Cell Diagnostics ...
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bioRxiv - Cancer Biology 2020Quote: ... The expression of proteins was detected using the Bond Polymer Refine Detection kit (Leica Biosystems, Cat# DS9800) with diaminobenzidine as chromogen (88) ...
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bioRxiv - Pathology 2021Quote: ... CA) was used with the Leica RNAScope 2.5 LS Assay-RED kit and a hematoxylin counterstain (Leica Biosystems, Richmond, IL). Slides were treated in protease (Advanced Cell Diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... with RNAscope 2.5 LS Assay Reagent Kit-Red (322150, Advanced Cell Diagnostics) and Bond Polymer Refine Red Detection (DS9390, Leica Biosystems). Control probes detecting a validated positive housekeeping gene (mouse peptidylprolyl isomerase B ...
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bioRxiv - Cancer Biology 2023Quote: ... with RNAscope 2.5 LS Assay Reagent Kit-Red (322150, Advanced Cell Diagnostics) and Bond Polymer Refine Red Detection (DS9390, Leica Biosystems). Control probes detecting a validated positive housekeeping gene (mouse peptidylprolyl isomerase B ...
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bioRxiv - Microbiology 2024Quote: ... with RNAscope 2.5 LS Assay Reagent Kit-Red (322150, Advanced Cell Diagnostics) and Bond Polymer Refine Red Detection (DS9390, Leica Biosystems). Control probes detecting a validated positive housekeeping gene (mouse peptidylprolyl isomerase B ...
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bioRxiv - Pathology 2022Quote: ... CA) was detected using the Leica RNAScope 2.5 LS Assay-RED kit with a hematoxylin counterstain (#322150, Leica Biosystems, Richmond, IL). An RNApol2 probe served as a host gene control to evaluate RNA quality ...
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bioRxiv - Immunology 2024Quote: FRAP assay was conducted by Leica TCS SP8 STED 3X confocal microscopy ...
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bioRxiv - Genomics 2024Quote: ... The nuclei were differentiated with mild acid treatment (Leica Biosystems 3803598), followed by “bluing” through exposure to a weakly alkaline solution (Leica Biosystems 3802918 ...
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bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
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bioRxiv - Neuroscience 2024Quote: ... myoinositol (3) and ascorbic acid (0.5) using a vibratome (Leica VT1200, Germany). Brains slices were transferred to an interface type holding chamber filled with 28 °C carbogen saturated ASCF containing (in mM) ...
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bioRxiv - Microbiology 2024Quote: ... and the RNAscope® ISH assay was performed using the RNAscope 2.5 LSx Reagent Kit (ACD) on the automated BOND RXm platform (Leica Biosystems, Buffalo Grove, IL, USA). Following automated baking and deparaffinization ...
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bioRxiv - Cancer Biology 2024Quote: Drug sensitivity assays were performed using THUNDER Imager Live Cell & 3D Cell Culture & 3D Assay imaging system (Leica-Microsystems, Wetzlar, Germany). Cells treated with different concentrations of Ketoconazole ...
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bioRxiv - Neuroscience 2023Quote: ... titrated to pH 7.4 with concentrated hydrochloric acid) using a VT1200s vibrating microtome (Leica). The transcardial perfusion procedure with 30 ml of chilled NMDG-HEPES aCSF was performed on P60 animals prior brain dissection and slicing ...
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bioRxiv - Bioengineering 2023Quote: ... Nonspecific protein-protein interactions were blocked with Leica PowerVision IHC/ISH Super Blocking solution (Leica PV6122) at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by decalcification of bone in a formic acid solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a Leica ASP6025 tissue processor ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein-protein signal was scanned and scored digitally by a pathologist with Aperio image scope software (Leica Microsystems).
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bioRxiv - Cancer Biology 2024Quote: ... for RNA assays and citrate based solution (ER1 Leica Cat. AR9961) for protein assays on a Leica Bond Rx Autostainer ...
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bioRxiv - Cancer Biology 2022Quote: ... p53 (1:2500, Leica Biosystems, P53-PROTEIN-CM5), p21 (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... femur and tibia were decalcified in a formic acid and formaldehyde solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a tissue processor (Leica ASP6025 ...
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bioRxiv - Cell Biology 2021Quote: The assay was performed using a confocal microscope (SP8, Leica, Wetzlar, Germany) with a controlled environment (37°C ...
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bioRxiv - Neuroscience 2023Quote: ... All in situ hybridization assays were imaged using a confocal (Leica SP5) or epifluorescence (Nikon Eclipse 90i ...
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bioRxiv - Bioengineering 2021Quote: ... The uptake of the fatty acids was monitored in real-time using a fluorescence microscope with incubation (Leica DMi8 with incubation unit ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were rinsed in tap water and dipped 3-5 times in 0.5% Acid Alcohol (Leica Biosystems 3803651), followed by rinsing in tap water and bluing with Scott’s Tap Water (Electron Microscopy Sciences 2607007 ...
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bioRxiv - Microbiology 2024Quote: ... and tibia were decalcified in a formic acid and formaldehyde solution (Surgipath Decalcifier I, Leica Biosystems, Wetzlar, Germany). All fixed tissues were then processed in ethanol and xylene in a tissue processor and embedded in paraffin (Leica ASP6025 ...
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bioRxiv - Cell Biology 2024Quote: Brightfield images (H&E, Acid phosphatase) were collected via a DM4000B upright microscope (Leica Microsystems, Milton Keynes, UK) using either a 10x or 20x objective (HC PL FLUOTAR PH1/PH2 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-glial fibrillary acidic protein (1:400, Leica Biosystems); anti-Iba1 (1:1,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-p53 (1:1000, Leica Biosystems, P53-PROTEIN-CM5), anti-p21 (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ... All assays were conducted using a TCS SP8 confocal microscope (Leica, Solms, Germany). RFU were calculated using Fiji Software.
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bioRxiv - Neuroscience 2022Quote: ... frozen sapphire sandwiches were incubated acetone containing 0.1% tannic acid at –90 °C for 20 – 24 h inside an AFS machine (Leica) under constant agitation ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were then incubated with DAPI for nucleic acid staining and imaged using a SP8 fluorescence microscope (Leica).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the skull was decalcified in a formic acid and formaldehyde solution (Surgipath Decalcifier I, Leica Biosystems, Deer Park, IL). Tissues were then processed in ethanol and xylene ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were stained with Hematoxylin-eosin or Periodic Acid Schiff (PAS) with the Leica BondMax Immunostaining System (Leica Biosystems). All tissue processing was performed by the Magee-Womens Research Institute histology and microimaging core ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-p53 (anti-p53 #p53-protein-cm5 Leica Biosystems) were used at 1:200 dilution ...
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bioRxiv - Microbiology 2022Quote: ... The apple tissue containing acervuli were stained with cotton blue in lactic acid and examined with a Leica DM2000 LED microscope (Leica Microsystems Switzerland ...
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bioRxiv - Neuroscience 2023Quote: ... A Leica DMi8 automated microscope with Thunder 3D assay imaging software (Leica Microsystems, IL) was used to capture ...
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bioRxiv - Neuroscience 2024Quote: ... individual worms were then pipetted onto assay plates using a dissecting microscope (Leica S9E).
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bioRxiv - Microbiology 2024Quote: ... Multiplex Fluorescent Assay (ACD, Cat# 322800) using the Bond RX auto-stainer (Leica Biosystems) with a tissue pretreatment of 30 minutes at 100°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed with assay buffer and imaged on a MICA Microhub (Leica).
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bioRxiv - Microbiology 2021Quote: ... as follows: SARS-CoV-2 Spike protein: 1:1000 (Leica, AR9961) 30minutes ...
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bioRxiv - Cell Biology 2023Quote: ... slides were incubated with antibodies to p53 protein (CM5) (Leica Biosystems), EpCAM (CD326 ...
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bioRxiv - Immunology 2024Quote: ... and blocked for 30 min with Protein Block (Leica Novocastra, UK). Primary antibodies were added without washing the blocking solution and incubated overnight at 4 0С ...
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bioRxiv - Bioengineering 2021Quote: ... The uptake of the fatty acids was monitored in real-time using a fluorescence microscope with incubation (Leica DMi8 with incubation unit, Leica Microsystems) for 60 min ...
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bioRxiv - Bioengineering 2021Quote: ... gravid day 1 adults animals were picked onto prepared palmitic acid plates and plates were imaged at 2 and 5 hours at 1.6x on a stereomicroscope (Leica M165 FC) using a 1.3 MP CMOS camera (Thorlabs DCC1645C ...
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bioRxiv - Cancer Biology 2020Quote: ... In regions with CCF two to four consecutive cryosections with a thickness of 12 µm were put on membrane-slides (Leica Frame Slides, Nuclease and human nucleic acid free, PET-Membrane, 1,4 µm, Leica, Wetzlar, Germany) and incubated for one minute in 70% ethanol at −16°C ...
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bioRxiv - Plant Biology 2024Quote: ... The leaf sections were then mounted with Polyvinyl Alcohol-Lactic acid (PVA-L) medium (8% v/v) microscopy glue and observed under light microscope (Leica DM5500B). Pycnidia width was calculated using the custom scale bar in Leica DM5500B microscope ...
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bioRxiv - Plant Biology 2020Quote: ... Images of the growth assays were taken with a THUNDER Imager Model Organism Microscope (Leica) using a greyscale camera ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a proprietary automatic staining assay developed for the BOND RX automated stainer (Leica Biosystems Division of Leica Microsystems Inc ...