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Citations for Leica :
1 - 50 of 194 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: Three independent positions per gel were acquired at the gel center using a confocal microscope (Leica SP5, Germany). For a general quantification of cell morphology ...
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bioRxiv - Immunology 2023Quote: ... the tissues were frozen using a cryo-gel embedding compound (Surgipath Cryo-Gel Embedding Compound for Frozen Section Leica, Ref: 39475237). Tissue sections with a size of 15-20 microns were obtained using a cryostat device (Leica CM1950 Cryostat) ...
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bioRxiv - Bioengineering 2020Quote: ... Gels were imaged using confocal microscopy (Leica TCS SP8).
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bioRxiv - Developmental Biology 2022Quote: ... Hearts were embedded in Surgipath Cryo-Gel (Leica, 39475237) the following day ...
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bioRxiv - Immunology 2023Quote: ... Gels were kept in PBS and imaged by Leica SP8 confocal microscopy ...
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bioRxiv - Cell Biology 2023Quote: ... The livers were then embedded and frozen in Cryo-Gel (Leica #39475237), and sectioned at a thickness of 16μm ...
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bioRxiv - Cancer Biology 2024Quote: ... gel and cut into 800μm-thick slices with a vibratome (Leica VT1200S). The slices farthest from the injection point were placed in an home-made polydimethoxysilane mold on a glass slide ...
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bioRxiv - Neuroscience 2020Quote: ... fixed in agarose gel (1.8%) and mounted on a vibratome (VT1000 A; Leica). Sagittal spinal cord slices (270 μm in thickness ...
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bioRxiv - Neuroscience 2022Quote: ... and mounted in Cryo-Gel Tissue Embedding Medium (Leica Biosystems GmBH, Wetzlar, Germany). Coronal brain sections (20-µm thick ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue gel hybrid was then resliced using a vibratome (VT1200, Leica Biosystems, Germany) in 75 μm (44 μm original ...
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bioRxiv - Biochemistry 2024Quote: ... Reaction products were resolved on 10-20% SDS-PAGE gels and visualized using LAS4000 (Leica), and stained with Simply Blue (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2024Quote: ... The coverslip containing the gel was then transferred to the microscope (Leica camera AG, Germany). A hollow glass capillary with an inner diameter 0.75mm (World Precision Instruments ...
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bioRxiv - Plant Biology 2021Quote: ... Calli embedded in each sodium alginate gel were counted under a dissection microscope (M165 FC, Leica) at 2.5× magnification ...
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bioRxiv - Cell Biology 2022Quote: ... The hearts were then cryopreserved in a sucrose gradient and embedded in Surgipath Cryo-Gel (Leica). Ten to twelve micrometer cryosections were prepared and consecutive sections were used for Acid Fuchsin Orange G (AFOG ...
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bioRxiv - Neuroscience 2023Quote: ... the brains were frozen in OCT gel (Tissue-Tek®) and sliced using a cryostat (Leica) into 50um slices ...
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bioRxiv - Developmental Biology 2023Quote: Fixed brains were embedded in 3% noble agar gel and sectioned with the Vibratome VT1000S (Leica) at 50 μm thickness as free-floating sections ...
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bioRxiv - Bioengineering 2020Quote: ... Gels were washed twice with PBS and subsequently imaged using confocal microscopy (Leica TCS SP8, Wetzlar, Germany) at 40X magnification.
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bioRxiv - Immunology 2023Quote: ... Cryoprotectant brains were then included in 4% agarose gel and sectioned sagitally on a vibratome (Leica, VT1200) at 40μm thickness ...
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bioRxiv - Biochemistry 2021Quote: ... proteins fixed in 30% ethanol/15% acetic acid and the gel piece embedded in tissue embedding media (Leica). After careful mounting on a cryo-holder ...
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bioRxiv - Plant Biology 2023Quote: Roots fixed with 2% (v/v) formaldehyde in Tris were embedded in Cryo-Gel (Leica Biosystems, ID:39475237) and cut onto a cryostat (Leica CM1950 ...
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bioRxiv - Cancer Biology 2024Quote: ... or frozen at –80 °C in optimal cutting temperature gel (OCT) for cryosectioning at 16 μm (Leica CM3050S). Permeabilization and blocking were done in PBS with 5% normal goat serum (NGS ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-p53 (anti-p53 #p53-protein-cm5 Leica Biosystems) were used at 1:200 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... anti-P53 (Leica biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Organoids embedded in the agarose gel were then sectioned into 500 μm thick slices with a vibratome (Leica VT1000S) using an advance speed of one and a vibration speed of nine ...
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bioRxiv - Plant Biology 2022Quote: ... Stained seed coats were fixed with 4-5% agarose gel and dissected by microtome (Leica, VT1000 S, Wetzlar, Germany) and were visualized by Nikon 80i-epi-microscope ...
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bioRxiv - Bioengineering 2023Quote: ... This tissue-gel block was glued onto the slicing platform of a vibratome (VT 1000S, Leica vibratome, Wetzlar, Germany) and sliced in 100 µm thick sections ...
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bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
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bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were embedded in a 5% low-gelling agarose gel (in PBS) and 50µm coronal slices were cut using a vibroslicer (Leica VT1000S). Slices were then stored in PBS-Azide (0.02-0.05% ...
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bioRxiv - Systems Biology 2020Quote: ... ‘Force- loaded’ images (with cells) of the beads embedded in the polyacrylamide gels were obtained using a 40X oil-immersion objective (Leica Microsystems). The ‘null-force’ image was obtained at the end of each experiment by adding trypsin to the cells for 1 hour ...
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bioRxiv - Biophysics 2020Quote: ... ‘Force-loaded’ images (with cells) of the beads embedded in the polyacrylamide gels were obtained using a 60× oil-immersion objective (Leica Microsystems). The ‘null-force’ image was obtained at the end of each experiment by adding trypsin to the cells for 1 h ...
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bioRxiv - Developmental Biology 2022Quote: ... The fixed tails were placed in 1 × PBS solution for 2 hours and then embedded in 7% agarose gel (1 × PBS as the medium) and cut into 180 μm horizontal sections by a vibratome (LEICA VT1000S). All lizards were released at the exact location of capture in less than 4 hours.
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bioRxiv - Neuroscience 2023Quote: ... embedded in Cryo-Gel (Cat. #:475237; Instrumedics Inc.) and sectioned along the modiolus axis using a cryostat slicer (Leica CM3050 S, Leica Biosystems), at the thickness of 20 μm.
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence images of the cells on polyacrylamide gels obtained using DMI 8 were improved using a 3D non-blind deconvolution method in the LAS X software (Leica Microsystems).
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bioRxiv - Neuroscience 2023Quote: ... left in 4% PFA for 24h to post-fix in a refrigerator and then embedded in blocks of 1.5% agarose gel before collecting slices at 70 micrometre thickness using a vibratome (Leica VT1000 S). Slices were then stained with DAPI for 15 min (1:1000 solution) ...
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bioRxiv - Bioengineering 2023Quote: ... Z stack Photos of the gel surface were taken using a fluorescence inverted microscope (SP8 LIGHTNING Confocal Microscope, Leica, Wetzlar, Germany) and the fluorescence intensity was quantified using LAS X(V 1.4.5 27713 ...
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bioRxiv - Bioengineering 2023Quote: ... Cross sectional Z-stack photos of gels were taken using a fluorescence inverted microscope (SP8 LIGHTNING Confocal Microscope, Leica, Wetzlar, Germany), and the fluorescence intensity was quantified using LAS X (V 1.4.5 27713 ...
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bioRxiv - Bioengineering 2022Quote: ... collagen gel was visualized using second harmonic generation (SHG) and full-spectrum multiphoton fluorescence lifetime imaging system (Leica TCS SP8 DIVE FALCON). Z-stacks separated no more than 0.5 μm were collected ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-p53 (Leica, CM5P), mouse anti-γH2AX ...
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bioRxiv - Neuroscience 2021Quote: Anti-dystrophin: Dys1 (Leica Biosystems), Mandys1 ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD8 4B11 (Leica, PA0183) and anti-CD68 514H12 (Leica ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti-p53NCL-p53-CM5 (Leica). Anti-Rb (IF8) ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue blocks were then embedded in 3% agarose gel and cut in the sagittal plane at 30 μm-thickness by the vibratome (Leica, Buffalo Grove, IL), counter-stained with Dapi (1:2000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... embedded in Cryo-Gel (Cat. #:475237; Instrumedics Inc.) and sectioned along the modiolus axis using a cryostat slicer (Leica CM3050 S, Leica Biosystems), at the thickness of 20 μm.
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bioRxiv - Developmental Biology 2021Quote: ... or 1:300 (rabbit anti-cleaved caspase 3, Cell Signaling Technology, 9665S; rabbit anti-p53, Leica Microsystems ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibodies were PowerVision Poly-HRP anti-rabbit or anti-mouse (Leica, PV6114 and PV6119) and developed with DAB.
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bioRxiv - Bioengineering 2020Quote: U87-MG migration across the microfluidic device was monitored daily by acquiring fluorescent images of the central gel channel with a DMi8 Yokogawa W1 spinning disk confocal microscope with a Hamamatsu EM-CCD digital camera (Leica Microsystems, Buffalo Grove, IL). Three regions of interest (ROI ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were embedded in 3% agarose gel in a cubic cryomold and coronally sectioned at 50 μm thickness using a VT1000S vibrating blade microtome (Leica Biosystems, Buffalo Grove, IL). The sections were rinsed in PBS ON at 4°C ...
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bioRxiv - Genomics 2021Quote: ... or anti-rabbit IgG (PV6119 Leica) secondary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... and anti-CD68 514H12 (Leica, PA0273).