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Citations for Leica :
1 - 50 of 571 citations for Aminomethylphosphonic Acid 13C 99%;15N 98%;Methylene D2 98% 100 Ug Ml H2O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... slides were dewaxed in xylene and rehydrated with EtOH series (100%, 90%, 80%, 70%, 50%, H2O) using an autostainer (Leica HistoCore Autostainer XL). Antigen retrieval was performed with high pH Tris-based Antigen unmasking solution (Vector Labs) ...
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bioRxiv - Immunology 2021Quote: ... 3 x H2O) on a linear stainer (Leica Biosystems, ST4020). Epitope retrieval was performed at 97C for 10 min with the Dako Target Retrieval Solution pH 9 (Agilent ...
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bioRxiv - Genetics 2020Quote: ... dyed with 0.5% methylene blue and observed in an optic microscope Leica DM200 (Leica Microsystems, Vienna, Austria). Ultrathin sections of 60 nm thick were obtained using a UC6 ultramicrotome (Leica Microsystems ...
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bioRxiv - Physiology 2019Quote: ... stained with methylene blue and observed at 40X magnification under a light microscope (DM6000 B, Leica Biosystems, Nussloch, Germany).
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bioRxiv - Molecular Biology 2021Quote: ... 1 μm thick sections were obtained and stained with Methylene Blue prior to obtaining ultrathin 70 nm sections using a Reichert Ultracut S ultramicrotome (Leica Microsystems). Sections were stained with lead and uranyl salts and images obtained using a Tecnai T12 microscope (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: ... Smears from infected CD71+ samples were stained with new methylene blue and examined under 100X oil immersion objective (Leica ICC50 W). Images of parasitic phenotypes were captured using a Leica digital camera24.
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bioRxiv - Cell Biology 2021Quote: ... freeze-substituted in acetone / 1% OsO4 / 5% H2O / 0.25% uranyl acetate (AFS2, Leica, Wetzlar, Germany) and embedded in Epon ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5% H2O) under liquid nitrogen and placed in an automatic freeze substitution system (Leica AFS2 ...
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bioRxiv - Cell Biology 2020Quote: ... acid alcohol (Leica) for 5 s ...
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bioRxiv - Microbiology 2020Quote: ... 2.3% methanol and 1–3% H2O in Acetone) tempered at −90°C in FS device (Leica AFS2) equipped with a robotic solution handler (Leica FSP) ...
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bioRxiv - Microbiology 2022Quote: ... sapphire discs placed in adapted carriers were filled with freeze substituent (0.1% uranyl acetate in acetone, 1% H2O) and placed in a temperature-controlling AFS2 (Leica) equipped with an FPS robot ...
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bioRxiv - Biochemistry 2023Quote: ... the grid was blotted for 1.5 s on the other side under 99% humidity and at 20 °C using EM GP2 plunge freezer (Leica) and immediately plunged into liquid ethane ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then transferred to 99% glycerol for imaging on a binocular microscope using Leica Application Suite (LAS 3.3, Leica).
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bioRxiv - Neuroscience 2021Quote: ... the pituitaries were embedded in 3% agarose (H2O) and para-sagittally sectioned with 60 μm thickness using a vibratome (Leica). From a single pituitary ...
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bioRxiv - Genetics 2020Quote: Third instar larvae expressing EOS-GFP were irradiated in H2O using a DAPI filter and 5x objective (Leica DMLB compound) for at least 2 minutes to ensure high conversion efficiency while maximising larval survival ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was embedded in 3% agarose (in H2O) and sectioned parasagitally with 70 μm thickness using a vibratome (Leica). The slices were mounted using Vectashield H-1000 Mounting Medium (Vector ...
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bioRxiv - Cell Biology 2021Quote: ... and freeze substituted in anhydrous acetone containing 1 % OsO4/ 2% H2O for 64h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in Epon ...
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bioRxiv - Cell Biology 2022Quote: ... and freeze substituted in anhydrous acetone containing 1% OsO4/2% H2O for 64 h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in EMbed 812 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5% H2O) under liquid nitrogen and placed in an automatic freeze substitution system (Leica AFS2, Leica Microsystems, precooled to −90 ℃) for 24 h ...
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bioRxiv - Cell Biology 2020Quote: Sample blocks were obtained by freeze substitution of the cell pellets in 0.5 % uranyl acetate diluted from a 20 % stock in methanol and 3 % double distilled H2O in acetone in a Leica AFS1 (Leica Microsystems GmbH, Wetzlar, Germany). Temperature was set to −130 °C for the first 2 hours and then raised to −90 °C with a 20 °C/hr gradient ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed and incubated in periodic acid (Leica 38016SS4A) followed by the Schiff Reagent (Fisher SS32-500).
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bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
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bioRxiv - Neuroscience 2023Quote: ... the mice were perfused intracardially with heparinized 0.1 M PBS (10 U/ml of Heparin, Ratiopharm, N68542.03; 100-125 mmHg pressure using a Leica Perfusion One system) for 5 minutes to remove the blood ...
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bioRxiv - Bioengineering 2021Quote: ... they were intracardially perfused with cold heparinized 0.1 M PBS (10 U/mL of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Cancer Biology 2019Quote: ... before intracardial perfusion with heparinized 0.01 M PBS (10 U/ml of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Cell Biology 2022Quote: ... 100 × 1.45 NA objective (Leica), and an Evolve 512Delta EMCCD (Teledyne Photometrics ...
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bioRxiv - Cell Biology 2020Quote: ... 100×1.45 NA objective (Leica), and an Evolve 512Delta EMCCD (Photometrics ...
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bioRxiv - Plant Biology 2023Quote: ... 100% ethanol) and incubated overnight in a 1:1 solution of 100% ethanol:Historesin solution (Leica). The solution was exchanged with Historesin and the samples incubated again overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... titrated to pH 7.4 with concentrated hydrochloric acid) using a VT1200s vibrating microtome (Leica). The transcardial perfusion procedure with 30 ml of chilled NMDG-HEPES aCSF was performed on P60 animals prior brain dissection and slicing ...
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bioRxiv - Cell Biology 2023Quote: ... using an HCX PL APO 100×100/1.40/0.70 oil immersion objective lens (Leica Microsystems, Mannheim, Germany). Emission depletion was performed with 592 nm CW STED laser ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by decalcification of bone in a formic acid solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a Leica ASP6025 tissue processor ...
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bioRxiv - Developmental Biology 2019Quote: ... β-Dystroglycan (1:100; Leica #NCL-b-DG), aPKCς clone C-20 (1:400 ...
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bioRxiv - Neuroscience 2022Quote: ... High-pressure freezing instrument (HPM 100, Leica) was used to preserve membrane structure ...
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bioRxiv - Neuroscience 2019Quote: ... and kynurenic acid 1 for 2-3 min and then glued on a vibratome VT1000S (Leica). The slicing chamber was filled with the same ice-cold aCSF ...
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bioRxiv - Bioengineering 2021Quote: ... two washes with 100% ethanol (Leica Biosystems, 3803686) for 1 min each ...
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bioRxiv - Cancer Biology 2020Quote: ... was diluted 1:100 in Antibody Diluent (Leica).
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bioRxiv - Cell Biology 2020Quote: ... Images were acquired at 100 times magnification (Leica), and the number of cells was counted with the software ImageJ ...
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bioRxiv - Molecular Biology 2023Quote: ... A ×100 oil-immersion objective (Leica, N.A. 1.4) was employed.
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bioRxiv - Neuroscience 2024Quote: ... 100-μm-thick free-floating vibratome (Leica VT1200) coronal sections were cut at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... femur and tibia were decalcified in a formic acid and formaldehyde solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a tissue processor (Leica ASP6025 ...
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bioRxiv - Cell Biology 2021Quote: ... samples were dehydrated in a graded ethanol series (50,70, 90, 100 and 100%) for 10 minutes each and then critical point dried (Leica CPD 300). Glass slips were adhered to a conductive carbon adhesive tab on aluminum stubs ...
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bioRxiv - Cell Biology 2021Quote: ... Osmicated samples were dehydrated in graded ethanols (25% to 100%) at 4°C and stored in 100% acetone until critical point drying with liquid CO₂ (EM CPD300, Leica Microsystems Ltd.). Samples were mounted onto stubs using silver paint (Agar Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... 100 μM thick coronal sections were cut (Leica, VT1200s). Sections containing LC were incubated with rabbit anti-Tyrosine Hydroxylase (TH ...
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bioRxiv - Cell Biology 2022Quote: ... sectioned with a vibratome (100 mm thickness; VT1200s; Leica) and immune stained ...
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bioRxiv - Neuroscience 2022Quote: ... using liquid nitrogen under high pressure (Leica HPM 100). Samples then underwent freeze substitution (Reichert AFS ...
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bioRxiv - Biochemistry 2023Quote: ... using a VCT 100 Vacuum Cryo Transfer device (Leica). After fracturing at a temperature of −120°C ...
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bioRxiv - Neuroscience 2023Quote: ... using liquid nitrogen under high pressure (Leica HPM 100). After freezing ...
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bioRxiv - Cell Biology 2023Quote: ... anti-α-SMA (NCL-L-SMA, Leica, 1:100), anti-SOX9 (ab5535 ...
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bioRxiv - Cancer Biology 2023Quote: ... F4/80 (Abcam ab6640, 1:100, Enzyme 1 Leica), CD45R (Abcam ab64100 ...
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bioRxiv - Neuroscience 2024Quote: ... sliced (∼100 μm) by vibratome (Leica VT1200S, Wetzlar, Germany) after post-fixed overnight to confirm the imaging position and virus expression.