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Citations for Leica :
1 - 50 of 341 citations for Alpinone 3 Acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2 ...
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bioRxiv - Microbiology 2022Quote: ... Grids were post-stained for 30 min with 2% uranyl acetate (Roth) and 20 min with 3% lead citrate (Leica). TEM images were acquired using a Zeiss Leo 912 Omega (Zeiss ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Cell Biology 2020Quote: Sample blocks were obtained by freeze substitution of the cell pellets in 0.5 % uranyl acetate diluted from a 20 % stock in methanol and 3 % double distilled H2O in acetone in a Leica AFS1 (Leica Microsystems GmbH, Wetzlar, Germany). Temperature was set to −130 °C for the first 2 hours and then raised to −90 °C with a 20 °C/hr gradient ...
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bioRxiv - Immunology 2023Quote: ... and stained with uranyl acetate and lead citrate (Leica AC20). Micrographs were collected on a JEM1010 (JEOL ...
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bioRxiv - Neuroscience 2024Quote: ... and stained in uranyl acetate and lead citrate (Leica EM AC20). Sections were imaged in a JEOL1010 transmission electron microscope (JEOL ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were stained in 2% aqueous uranyl acetate and lead citrate (Leica) and imaged on a Hitachi HT7800 transmission electron microscope ...
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bioRxiv - Cell Biology 2022Quote: ... After automated staining with uranyl acetate and lead citrate (Leica EM AC20), samples were observed via either Zeiss TEM 902 A (50 kV ...
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bioRxiv - Cancer Biology 2023Quote: ... stained with uranyl acetate and lead citrate in an EM Stainer (Leica), and examined using a JEM 1010 transmission electron microscope (JEOL ...
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bioRxiv - Plant Biology 2023Quote: ... post-stained with uranyl acetate and lead citrate with an EMSTAIN (Leica) and observed with a Libra 120 transmission electron microscope (Carl Zeiss Microscopy GmbH ...
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bioRxiv - Neuroscience 2021Quote: ... and sections post-stained with 0.5% uranyl acetate (Leica Ultrostain I, Leica Microsystems) and 3% lead citrate (Leica Ultrostain II ...
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bioRxiv - Neuroscience 2021Quote: ... and sections post-stained with 0.5% uranyl acetate (Leica Ultrostain I, Leica Microsystems) and 3% lead citrate (Leica Ultrostain II ...
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bioRxiv - Bioengineering 2023Quote: ... were afterwards stained for 30 min in 1% aqueous uranyl acetate (Leica, Germany) and 20 min in 3% lead citrate (Leica ...
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bioRxiv - Immunology 2022Quote: ... 3′-3-diaminobenzidine (DAB) substrate (Leica Microsystems) was used as a chromogen followed by hematoxylin counterstain ...
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bioRxiv - Cell Biology 2021Quote: ... freeze-substituted in acetone / 1% OsO4 / 5% H2O / 0.25% uranyl acetate (AFS2, Leica, Wetzlar, Germany) and embedded in Epon ...
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bioRxiv - Bioengineering 2022Quote: ... 3′-diaminobenzidine (Leica).
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.05% uranyl acetate in acetone and placed into an AFS-2 Freeze Substitution Machine (Leica Microsystems, Austria). Samples were freeze-substituted at −90°C for 72 h ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 % glutaraldehyde and 0.25 % uranyl acetate for 60 h in a FS system (AFS-2, Leica Microsystems). Larvae were embedded in an Epon-Araldite mix (hard formula ...
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bioRxiv - Cell Biology 2022Quote: ... 0.05% uranyl acetate in acetone and placed in an AFS-2 Freeze-Substitution Machine (Leica Microsystems, Vienna). Samples were freeze-substituted at -90°C for 72 h ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in an FS system (AFS-2; Leica Microsystems). The embryos were embedded in an Epon-Araldite mix (EMS hard formula) ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were contrasted with uranyl acetate and lead citrate in an ultra-stainer (Leica EM AC20) and imaged in a JEOL1010 transmission electron microscope (JEOL ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in a FS system (AFS-2, Leica Microsystems). Larvae were embedded in Epon-Araldite mix (EMS hard formula) ...
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bioRxiv - Neuroscience 2023Quote: ... post-contrast was realized with uranyl acetate and lead citrate in an ultra-stainer (Leica EM AC20).
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bioRxiv - Cell Biology 2020Quote: ... 0.05% uranyl acetate in acetone and then placed in a AFS-2 freeze substitution machine (Leica Microsystems, Vienna). Samples were freeze substituted at −90°C for 72 h ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were post-contrasted with uranyl acetate and lead citrate in an ultra stainer (LEICA EM AC20).
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bioRxiv - Microbiology 2020Quote: ... 0.05 % uranyl acetate in acetone and placed in an AFS-2 freeze-substitution machine (Leica Microsystems, Wetzlar, Germany). Tissues were freeze-substituted at −90 °C for 72 h ...
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bioRxiv - Pathology 2021Quote: ... These were double contrasted using aqueous 2% uranyl acetate (Honeywell International Inc.; Morristown) and lead citrate solutions (Leica) for 10 min each ...
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bioRxiv - Cell Biology 2022Quote: ... After subsequent freeze substitution (using acetone, containing 0.25% osmium tetroxide, 0.2% uranyl acetate, 0.05% ruthenium red, and 5% water) (Leica AFS2), cells were embedded in Epon812 substitute resin (Fluka) ...
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bioRxiv - Microbiology 2022Quote: ... sapphire discs placed in adapted carriers were filled with freeze substituent (0.1% uranyl acetate in acetone, 1% H2O) and placed in a temperature-controlling AFS2 (Leica) equipped with an FPS robot ...
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bioRxiv - Cell Biology 2020Quote: ... Sections mounted on Pioloform filmed copper grids prior to staining with 2% aqueous uranyl acetate and lead citrate (Leica). Ultrathin sections were examined on a Phillips CM 100 Compustage (FEI ...
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bioRxiv - Plant Biology 2022Quote: ... followed by freeze substitution in dry acetone containing 0.4% uranyl acetate at −85°C in a freeze-substitution unit (EM AFS2, Leica). After three acetone rinses at −85°C ...
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bioRxiv - Neuroscience 2023Quote: ... The freeze-substitution process was performed using a cocktail of 0.1% uranyl acetate in acetone and EM AFS2 machine with FSP robot for automated reagent handling (Leica). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... the high-pressure-frozen samples were freeze-substituted in 0.2% glutaraldehyde with 0.2% uranyl acetate in acetone at -90°C in an automated freeze-substitution device (Leica AFS). After 3 days ...
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bioRxiv - Biophysics 2023Quote: ... Sections were then stained with a Leica EM AC20 for 30 min in 0.5 % uranyl acetate (Ultrostain I, Leica) at 40 °C and for 7 min in 3 % lead citrate (Ultrostain II ...
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bioRxiv - Cell Biology 2023Quote: ... Vitrified samples were freeze-substituted in 1% osmium tetroxide/0.1% uranyl acetate in acetone (AFS2, Leica Microsystems, Vienna, Austria), and processed to Epon-Araldite resin as previously described.39 Tissue samples were stained with 1% osmium tetroxide ...
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bioRxiv - Molecular Biology 2021Quote: ... Worms were freeze substituted for 2 h at −90 °C with 1% osmium tetroxide and 0.1% uranyl acetate in acetone using a Leica EM AFS2 freeze substitution processor (Leica Microsystems). After a temperature ramp to 0°C ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were stretched with chloroform to eliminate compression and mounted on Pioloform filmed copper grids prior to staining with 1% aqueous uranyl acetate and lead citrate (Leica). They were viewed on a Philips CM100 Compustage Transmission Electron Microscope with images collected using an AMT CCD camera (Deben) ...
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bioRxiv - Cell Biology 2020Quote: ... Ultrathin sections of 60 nm were contrasted with uranyl acetate and lead citrate using an AC20 automatic contrasting system (Leica) and examined with a Jeol 1010 electron microscope (Jeol Europe) ...
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bioRxiv - Cell Biology 2020Quote: ... Ultrathin sections (50nm) were deposited onto formvar-coated copper grids (Plano) and postcontrasted using 1% uranyl acetate in water and ultrostain (Leica). TEM images were acquired on a JEM 1400plus (JEOL ...
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bioRxiv - Immunology 2021Quote: ... were cut and deposited onto formvar-coated grids (Plano) and again contrasted using 1 % uranyl acetate in water and Ultrostain (Leica). Images were acquired on a JEM 1400plus (JEOL).
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bioRxiv - Biochemistry 2022Quote: ... were transferred to formvar-coated copper grids and poststained with uranyl acetate and lead citrate in an EM-Stain apparatus (Leica) and subsequently observed with a Zeiss EM 900 transmission electron microscope (Carl Zeiss Microscopy GmbH ...
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bioRxiv - Neuroscience 2024Quote: ... Frozen sections were immersed in 0.5% uranyl acetate in methanol at –90°C in the AFS freeze-substitution unit (Leica Microsystems), infiltrated at –45°C with Lowicryl HM-20 resin (Chemische Werke Lowi ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were automatically stained on the grids with uranyl acetate and lead citrate (EM AC20; Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Cell Biology 2024Quote: ... Frozen pellets were immersed in 0.5% uranyl acetate in methanol at −90°C in an AFS freeze-substitution unit (Leica Microsystems), infiltrated at −45°C with Lowicryl HM-20 resin (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... prior to subsequent freeze substitution in acetone containing 0.4% uranyl acetate at −85 °C in an AFS freeze-substitution unit (Leica, Wetzlar, Germany). After gradient infiltration with increasing concentration of HM20 ...
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bioRxiv - Physiology 2021Quote: ... Gel-embedded platelets were stained with uranyl acetate and lead aspartate followed by dehydration with ethanol and infiltrated with Epon resin in a Tissue Processor (Leica EMTP). 70nm sections were cut from blocks with a Reichert Ultracut E and imaged with a Tecnai 12 electron microscope (ThermoFisher UK).
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bioRxiv - Biochemistry 2022Quote: ... the sections were applied on copper grids with formvar coating and uranyl acetate and lead citrate was added for post-staining in a specialized EM-Stain device (Leica, Germany). For imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 spectral detectors (HyD-RLD, Leica) and a 37°C preheated 40X objective (NA 1.10 ...
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at −90°C in anhydrous acetone containing 1% OsO4 and 0.1% uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were then embedded in a thin layer of resin and polymerized for 3 d at 60°C ...
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bioRxiv - Cell Biology 2020Quote: ... Freeze substitution was performed over 3 days at –90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria) Serial semi-thick sections (300nm ...