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Citations for Leica :
151 - 200 of 407 citations for ATPase H+ Transporting V0 Subunit A1 ATP6V0A1 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... The tissue was H&E-stained and imaged with a bright-field microscope scanner (Aperio Whole Slide Scanner, Leica Biosystems) for reference ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
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bioRxiv - Cell Biology 2019Quote: ... Single-cell tracks were acquired using live-cell imaging at 10 min intervals over 24 h (AS MDW live cell imaging system, Leica). Multiple position imaging was gathered using Image Pro 6.3 (Media Cybernetics Ltd ...
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bioRxiv - Neuroscience 2020Quote: ... One hemisphere was post-fixed by incubation for 48 h in 4% paraformaldehyde (PFA) and cut into 35 μm sections with a vibratome (Leica) for histological analyses (brains used for RNAscope were perfused with both PBS and 4% PFA prior post-fixation and cut in 10 μm sections with a cryostat) ...
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bioRxiv - Immunology 2022Quote: ... in a blinded manner on 1 µm thick microtome sections of paraffin-embedded whole-lungs stained with H&E and scanned at a 40x magnification using a bright-field scanner (Aperio AT2, Leica). Morphological changes and immune cell infiltration in the lungs were graded as previously described (Li et al ...
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bioRxiv - Physiology 2022Quote: ... eight images were randomly captured at 20X magnification along the length of an H&E stained SI section from each dam (Leica DMIL LED inverted microscope ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated at 33° C for the for at least 24 h before being imaged with a fluorescence on a microscope (Leica).
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bioRxiv - Microbiology 2021Quote: ... Images were acquired every 15 min for 16 h using a Leica DMi-8 epifluorescent microscope fitted with a DFC365 FX camera (Leica) and Plan-Apochromat 63x oil objective (HC PL APO 63x/1.4 Oil ph3 objective Leica) ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μm sections were taken for H&E staining using standard methods and imaged using a DMI 6000B microscope (Leica). To examine tdTomato expression in sections ...
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bioRxiv - Cancer Biology 2022Quote: Hematoxylin and Eosin (H&E) staining was performed on 5 μm thick slices using an automated system (Leica Autostainer, ST5010) with the protocol as described in Supplemental Table 1 ...
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bioRxiv - Biochemistry 2022Quote: ... Images were acquired every 15 min at 30°C for 17 h using a Leica DMi-8 epifluorescent microscope fitted with a DFC365 FX camera (Leica) and Plan-Apochromat 63x oil objective (HC PL 614 APO 63x/1.4 Oil ph3 objective Leica) ...
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bioRxiv - Microbiology 2022Quote: ... and one-way repeated measures ANOVA (Figs. 1D–F, and 2C-H). Syncytia sizes (Figs. 3B, 4B, and 4D) were quantified by Leica Applications Suit X (Wetzlar ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Time-lapse microscopy of developing genitalia was performed at 20 – 25 h after the puparium had formed on a DMI8 inverted microscope (Leica), equipped with a spinning-disc head CSU-W1 (Yokogawa Corporation of America ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Brains were removed and post-fixed in 4 % PFA in PBS at 4 °C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm ...
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bioRxiv - Biochemistry 2023Quote: ... The H-E-stained tissue sections were visualized and exported from Aperio ImageScope (version 12.4.6, Leica Biosystems, Deer Park, IL).
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bioRxiv - Immunology 2023Quote: ... Samples were hardened for 48 h and ultrathin sections were collected using a Leica UC6 ultramicrotome (Leica Microsystems, Wetzlar, Germany). The sections were imaged at 80 kV in Hitachi 7800 microscope (Hitachi High-Tech in America ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with either H&E or specific immunohistochemistry markers (HA-Tag C29F4 #3724 from Cell Signaling Technology; GranzymeB Leica Biosystems PA0291 ...
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bioRxiv - Plant Biology 2023Quote: ... GFP signals were detected at different time intervals between 48-96 h post infiltration (hpi) with the TCS SP8 microscope (Leica).
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bioRxiv - Neuroscience 2023Quote: ... was cryoprotected with 30% sucrose in PBS 0.1 M for 48 h and cut afterwards in 50-µm-thick coronal sections using a sliding microtome (LEICA SM2000R, Leica). Slices were collected in 6 subseries and stored at −20°C in a cryoprotective solution (30% glycerol ...
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bioRxiv - Physiology 2024Quote: ... The images of the H&E stained pathology slides were taken using a digital pathology slide scanner (Leica, Aperio CS2) and analyzed by one of us (Dr ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by secondary antibodies (Leica) and stained with the Bond Polymer Refine Detection Kit (DS9800 ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies were diluted to their optimal staining concentration in Bond primary antibody diluent (Leica, AR9352) as follows ...
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bioRxiv - Cell Biology 2020Quote: ... Automated freeze-substitution in 0.1% uranyl acetate in acetone over a 72 h period and embedding in Lowicryl HM20 resin were performed using an EM AFS2 (Leica Microsystems) as previously described (Kukulski et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were embedded at 60°C for 48 h and cut into 70 nm sections with a microtome (EM UC7, Leica, Germany). After electron staining with uranyl acetate and lead citrate ...
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bioRxiv - Molecular Biology 2020Quote: Cells expressing EGFP protein were visualized 48 h post-transfection by fluorescent microscopy using Leica DMi8 inverted microscope (Leica Microsystems, Germany) at magnification 10x ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections of TA and liver were stained with hematoxylin and eosin (H&E) using an XL CV5030 autostainer (Leica, Wertzler, Germany). Samples stained with H&E were scanned with a MIRAX MIDI digital slide scanner (Zeiss ...
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bioRxiv - Microbiology 2022Quote: ... and were stained with hematoxylin and eosin (H&E) and coverslipped using a ST5010-CV5030 stainer/coverslipper combination (Leica, Wetzlar, Germany). Slides were examined blinded by a board-certified veterinary pathologist (N.A.C.) ...
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bioRxiv - Biochemistry 2022Quote: ... 36 h post-transfection both the cell lines were trysinised and co-cultured in 1:1 ratio for 12 h followed by fixing permialization and subsequent imaging using fluorescence microscope (Leica Microsystems) and subsequent analysis by ImageJ software.
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bioRxiv - Microbiology 2022Quote: ... the samples were freeze substituted at −90 °C for 80 h in acetone supplemented with 1% OsO4 and warmed up slowly (1°C h−1) to −60 °C in an automated freeze substitution device (AFS2; Leica Microsystems). After 8 to 12 h ...
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bioRxiv - Bioengineering 2022Quote: ... The gels were subsequently incubated with EM containing 10 μM forskolin (Wako) with DMSO or Inh-172 for 24 h before imaging with a Leica DMi8 (Leica Microsystems) using the Leica Application Suite X (LAS-X ...
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bioRxiv - Cancer Biology 2022Quote: ... spheres were incubated in n-Butanol (three times 30 min) and melted paraffin (three times) before they were Paraffin-embedded using the HistoCore Arcadia H (Leica Biosystems) following the manufacturer’s protocol in a medium size mold ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Plant Biology 2020Quote: ... by infiltration for 10 min in a vacuum and after further incubation for 4 h processed using the Leica Semi-Enclosed Benchtop Tissue Processor TP1020 (Leica Biosystems) for an ethanol series (75 % ...
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bioRxiv - Cell Biology 2021Quote: ... The enucleated eyes were further fixed in the same fixative for 24 h and cut on a vibratome (VT1000S, Leica, Germany) at 200μm thickness.
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bioRxiv - Physiology 2021Quote: ... The lung tissue sections of 3-5 micron were stained with H&E stain and were microscopically analyzed using microscope (Make: Leica DM1000 LED; Camera: Leica MC170HD). The images were taken with 10X objective lens (Magnification X 100 ...
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bioRxiv - Biochemistry 2020Quote: ... H&E staining on 10 µm frozen section was performed using the Leica ST5020 Multistainer workstation (Leica Biosystems, Buffalo Grove, IL) according manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... tissues were fixed in neutral buffered formalin (NBF) for 48 h prior to processing using standard protocols in a vacuum infiltration processor (Leica ASP300S). Tissue sections were embedded in paraffin and stained with hematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA for 48 h at 4 °C and cut into 60 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Neuroscience 2022Quote: ... timelapse phase contrast images were acquired for 2 h at 37 °C with a 10X or 20X fluotar objective on a Leica DMIRE2 inverted microscope (Leica, Germany) equipped with a MS-2000 XYZ automated stage (ASI ...
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bioRxiv - Pathology 2022Quote: Five micron sections of formalin-fixed and paraffin-embedded (FFPE) specimens were H&E-stained by using an automated stainer (Leica XL) at the University of Manchester Histology Core Facility as previously described [19] ...
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bioRxiv - Neuroscience 2022Quote: ... Viral titers were determined by transduction of HEK293T cells for 72 h with limiting dilution of MMLV suspension and counting of reporter expressing cell spots under a fluorescent microscope (Leica Microsystems). Titers applied were ~5 × 108 colony-forming units (CFU)/ml (Zhao et al. ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 1 h in a 1:1 solution of 100% ethanol and solution A (Leica Historesin Embedding kit). After 2 h in solution A ...
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bioRxiv - Cell Biology 2022Quote: ... and freeze substituted in anhydrous acetone containing 1% OsO4/2% H2O for 64 h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in EMbed 812 ...
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bioRxiv - Neuroscience 2023Quote: ... The fixed right brain hemisphere tissues were dehydrated using a vacuum infiltration processor (Excelsior ES, Thermo, USA) and embedded in paraffin using an Arcadia H heated embedding workstation (Leica, Germany).
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bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with half seed in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems). with vacuum infiltration ...
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bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with dozens of seeds in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems) with vacuum infiltration ...
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bioRxiv - Plant Biology 2023Quote: ... they were kept at 28±1°C in dark for 16 h and observed under confocal microscope (Leica microsystems, TCS, SP5). BiFC for each combination was repeated twice and appropriate negative controls were used (Kudla and Bock ...
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bioRxiv - Biochemistry 2022Quote: ... H&E staining on 10 μM frozen section was performed using the Leica ST5020 Multistainer workstation (Leica Biosystems, Buffalo Grove, IL) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... A 680 nm short pass filter and a 560 nm long pass dichroic mirror were used to respectively reduce the laser scattering and to split the NAD(P)H and FAD fluorescence toward two hybrid photomultiplier detectors (HyD, Leica Microsystems). 460/60 nm ...