Labshake search
Citations for Leica :
201 - 250 of 258 citations for 8 Bromoacetylquinoline hydrobromide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... The bright-field and immunofluorescent images were acquired using a Pannoramic MIDI system (3DHISTECH) and confocal microscopy (STELLARIS 8 WLL, Leica).
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bioRxiv - Neuroscience 2023Quote: ... Digital images of DAB-stained sections were acquired using a whole slide scanner (3D-Histech Pannoramic 250 Flash III, Sysmex Deutschland GmbH, Norderstedt, Germany or Aperio Versa 8, Leica Biosystems Nussloch GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... The fresh Phenol Red-free DMEM media with 10% FBS was added prior to confocal microscopic experiment (Leica Stellaris 8). A 405 nm laser excitation was used to image BFP and a 488 nm laser excitation was used to image EGFP.
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bioRxiv - Microbiology 2023Quote: ... The emitted fluorescence resulting from Fluo-8 or Rhod-2 AM staining was observed using a fluorescence microscope (DM6000B,Leica) with a 488-nm or 543-nm filter ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed tissues were embedded in CryoMount I (Muto PureChemicals) and 8 μm-thick sections were cut using a cryostat (Leica). For immunofluorescence assay ...
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bioRxiv - Physiology 2024Quote: Cardiac tissue was embedded in Optimal Cutting Temperature (OCT) (Tissue Tek) and cut into 8 μm thick using a cryostat (CM1850; Leica) with the temperature set as −20°C ...
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bioRxiv - Bioengineering 2024Quote: ... microscope or in the transmitted light and in the RFP channel (580 nm) on a Stellaris 8 microscope using the LAS X software in both cases (Leica).
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bioRxiv - Biophysics 2024Quote: ... Images of live embryos were captured using the LAS X Life Science Microscope Software with the Stellaris 8 FALCON (Leica) equipped with an HC PL APO 100x/1.40 OIL STED WHITE objective lens and a white light laser source ...
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bioRxiv - Cell Biology 2020Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). Platinum replicas were floated off the glass by 5% hydrofluoric acid ...
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bioRxiv - Developmental Biology 2021Quote: ... EBs or brain tissues were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica, 14020108926). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... bright-field photomicrographs of 15 wild-type larvae across three clutches were taken at each developmental time-point using an 8 megapixel iSight camera (Apple) through the ocular of a stereoscope (M80, Leica Microsystems). Larvae were immobilized dorsal up in 2% lowmelting temperature agar (Thermo Fisher Scientific 16520) ...
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bioRxiv - Plant Biology 2021Quote: ... Root segments were embedded in Technovit 7100 (Hereus-Kulzer, Germany) and cut into 8 μm longitudinal sections using a microtome (Leica RM2255) and stained with 0.1% Ruthenium Red for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... A block containing the hippocampus was dissected out of the remaining un-sectioned tissue then paraffin-embedded and sectioned transverse to the long axis of the hippocampus at 8 μm using a rotary microtome (Leica RM2235) and collected directly onto SuperFrost plus slides for spatial transcriptomics.
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bioRxiv - Molecular Biology 2020Quote: ... Tissue specimens from two patients for each disease histotype were sectioned (8 µm) by microtome onto polyethylene naphthalate membrane slides (Leica Microsystems) and hematoxylin-eosin stained ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated in 1 mL experimental buffer and placed inside the chamber of a Leica DMi‐8 microscope equipped with a Fura2 filter (Leica Microsystems) and a 40× objective ...
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bioRxiv - Cell Biology 2019Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Developmental Biology 2019Quote: ... heat shocked around 31 hpf at 39.5 °C for 30 min in a water bath and imaged 35 min after the end of the heat shock every 20 min for 8 hours on a Leica SP8 confocal microscope equipped with HyD detectors (Leica Microsystems) using a 40x (NA 0.8 ...
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bioRxiv - Developmental Biology 2019Quote: ... cxcr4b -/- embryos with and without the hsp70l:zGrad-IRES-h2a-TagBFP transgene were heat shocked at 31 hpf and 39.5 °C for one hour in a water bath and imaged 30 min after the end of the heat shock every 20 min for 8 hours on a Leica SP8 confocal microscope equipped with HyD detectors (Leica Microsystems) using a 20x (NA 0.5 ...
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bioRxiv - Plant Biology 2021Quote: ... The GFP fluorescence in the cells was detected using a confocal laser scanning microscope (FluoView 300, OLYMPUS, Japan and STELLARIS 8, Leica, Germany) or a fluorescence microscope (THUNDER Imager ...
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bioRxiv - Neuroscience 2022Quote: Fresh-frozen brains from adult male C57BL/6J mice (8-12 weeks) were sectioned at a thickness of 16 µm using a Cryostat (Leica Biosystems). Sections were collected onto Superfrost Plus slides (Daigger Scientific ...
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bioRxiv - Immunology 2023Quote: ... cut into 4 groups of 8 sequential 7-μm-thick sections separated by a depth of 150-μm using a Leica CM1860 UV cryostat (Leica Microsystems), and mounted as duplicates on Fisherbrand ProbeOn Plus slides glass slides (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... or the donor only controls were imaged on 25% gelatin pad at room temperature on a Stellaris 8 Falcon laser scanning microscope (Leica, Germany) equipped with a 100X objective with 20% 440nm laser excitation at 600 speed ...
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bioRxiv - Bioengineering 2023Quote: ... Force maps were measured using a bio-AFM Nanowizard 4XP (Bruker-JPK, Germany) placed on a Leica DMi 8 inverted microscope with a 10x objective (Leica, Germany). A 5.73 µm melamine sphere (microParticles ...
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bioRxiv - Developmental Biology 2023Quote: ... confocal photomicrographs were taken at the level of the caudal fin with TCS-SP2 spectral or Stellaris 8 confocal laser microscopes (Leica Microsystems) with a 20x objective ...
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bioRxiv - Neuroscience 2023Quote: ... 20X (0.8 NA) 16 bit images of MCU and RGS14 immunolabeling were acquired on a Leica Thunder epifluorescence microscope (Leica DMi 8) using identical acquisition parameters for CTL and cKO and Lightning computationally clearing ...
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bioRxiv - Bioengineering 2023Quote: ... tissue blocks were embedded into optimal cutting temperature compounds (Sakura Finetek) and co-sectioned (8 μm) on a Cryostat instrument (Leica Biosystems) to prepare tissue sections ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence images of the cells on polyacrylamide gels obtained using DMI 8 were improved using a 3D non-blind deconvolution method in the LAS X software (Leica Microsystems).
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bioRxiv - Immunology 2023Quote: ... Transiently transfected HEK293 cells in 8 well chamber slide were directly imaged with a Leica SP8 inverted five channels confocal microscope (Leica Microsystems). HEK293 cells ...
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bioRxiv - Systems Biology 2023Quote: Frozen serial 8 μm sections of mice heart tissues were cut at −20°C with a Microtome (CM1950; Leica, Nussloch, Germany) and mounted on ITO-coated glass slides for MALDI-MSI and on coated glass slides for HE staining ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were then washed two times for 8 min in DPBS and mounted on slides (Leica Surgipath X-tra Adhesive, 3800202) using Vectashield Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... MMB were imaged by taking z-stacks (approximately 300 nm per image) of the entire consortia using an Inverted DMI8 Stellaris 8 Confocal Microscope (Leica Microsystems). Images focused on the center of the consortia were selected and Eman2 (88 ...
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bioRxiv - Pathology 2024Quote: ... The mt-Keima signals in epicardial myocytes were detected at 488 nm and 568 nm in situ with confocal microscope (STELLARIS 8, Leica Microsystems). The heatmaps representing 568/488 ratio were analyzed and generated using MATLAB software ...
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bioRxiv - Molecular Biology 2019Quote: ... sections from OCT-preserved samples were cut at a thickness of 8 μm using a cryotome (Leica Biosystems; Buffalo Grove, IL, USA) and were adhered to positively-charged histology slides ...
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bioRxiv - Neuroscience 2020Quote: ... of 2 μm between each plane (8 μm in total) were recorded and merged via the maximum projection tool in the LASX-software (Leica, Mannheim, Germany). The fluorescence intensity curves were measured along the cell nucleus within a region of interest (ROI ...
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bioRxiv - Physiology 2021Quote: Frozen heart sections embedded in OCT compound (Tissue-Tek®; Sakura, UAE) were cut into 8 µm sections with a cryostat (Leica, Germany), permeabilized ...
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bioRxiv - Microbiology 2022Quote: ... Single-cell resolution images of fixed samples were acquired using a DMI8 Leica SP-8 point scanning confocal microscope (Leica, Wetzlar, Germany) equipped with a 63x water immersion objective (Leica ...
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bioRxiv - Physiology 2021Quote: Liver tissue samples embedded in Tissue Tek (Weckert, Kitzingen, Germany) were cryosectioned (8 μm thick) using a Leica CM3050 S (Leica, Bensheim, Germany) cryostat microtome ...
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bioRxiv - Biochemistry 2021Quote: ... Femurs were cryosectioned at 8 μm thickness using a DB80 HS microtome blade in a CM3050 S cryostat (Leica Biosystems, Wetzlar, Germany) set to −25 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... (Sakura Finetek; Alphen an den Rijn, Netherlands) for cryo-sectioning at 8 µm sections using a CM1850 Cryostat (Leica Biosystems; Wetzlar, Germany).
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bioRxiv - Neuroscience 2022Quote: ... and a Leica STELLARIS 8 confocal microscope with a 20x HC PL APO NA 0.75 multi-immersion objective (Leica Microsystems, Wetzlar, Germany). Raw images were projected with Fiji (Schindelin et al ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the specimens were embedded in Technovit 8100 (Kulzer, Germany)100 and cut into 8 µm sagittal sections using a Leica HistoCore AUTOCUT R microtome (Leica, Wetzlar, Germany) equipped with glass knives ...
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bioRxiv - Biophysics 2019Quote: ... were cleaned with chloroform and hydrophilised by 60 s glow discharging at 8 W in a BAL-TEC MED 020 device (Leica Microsystems, Wetzlar, Germany), before 5 μl aliquots of the liposome/protein solution were applied to the grids ...
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bioRxiv - Neuroscience 2021Quote: ... Red light illumination was provided by 8 individually addressable LEDs (WS2812) attached to the objective (Leica Z6 APO coupled with a Leica PlanApo 2.0X 10447178) by a custom 3D-printed conical holder ...
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bioRxiv - Neuroscience 2021Quote: ... isogenic samples reared in the same environment were highpressure frozen (Leica HPM100 for datasets 1-5 and Leica ICE for datasets 6-8) at different stages of post-embryonic development ...
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bioRxiv - Plant Biology 2021Quote: ... Inflorescence stem subjected to GUS staining were then embedded in 8% agarose and sectioned with a Leica VT100S vibratome (Leica, https://www.leica-microsystems.com/). Sections were counterstained for lignin by phloroglucinol staining (Pradhan Mitra and Loqué ...
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bioRxiv - Plant Biology 2021Quote: ... GFP and YFP signals were imaged on a Leica SP2 or Leica Stellaris 8 Laser Scanning Confocal Microscope (Leica Microsystems, Buffalo Grove, IL) at 48hpi ...
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bioRxiv - Neuroscience 2019Quote: ... Coronal slices of paraffin sections with 8 µm thickness were continuously cut using a Leica RM2255 microtome (Leica Biosystems Inc. Buffalo Grove, IL) and mounted on Superfrost Plus microscope slides (cat# 12-550-15 ...
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bioRxiv - Neuroscience 2020Quote: ... and the brain was cut into 400-um thick coronal sections using a vibratome (Leica VT1000S; Leica, speed 5, vibration frequency 7-8), while in bubbling ice cold ACSF (recipe as above ...
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bioRxiv - Plant Biology 2022Quote: ... Inflorescence stems subjected to GUS staining were then embedded in 8% (w/v) agarose and sectioned with a Leica VT100S vibratome (Leica, https://www.leica-microsystems.com/). Sections were counterstained for lignin by phloroglucinol staining (Pradhan Mitra and Loqué ...
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bioRxiv - Neuroscience 2024Quote: ... High-resolution microphotographs (1024×1024 pixel resolution and 400Hz acquisition speed) were taken at 20X magnification using an inverted Stellaris 8 confocal microscope (Leica Microsystems, Wetzlar, Germany). Same laser gain ...