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Citations for Leica :
201 - 250 of 297 citations for 7 Methoxy 6 azaindole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Embedded tissues were sectioned into 6-µm sections and loaded into BOND RXm (Leica Biosystems, Wetzlar, Germany) for a series of staining including F4/80 ...
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bioRxiv - Neuroscience 2019Quote: ... unfixed C57BL/6 mouse brains (N = 3) were embedded in tissue freezing medium (Leica Biosystems, Richmond, UK), frozen on a dry ice ethanol bath ...
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bioRxiv - Neuroscience 2021Quote: Coronal sections of the rat brains were cut at 6 μm using a microtome (Leica RM2245, Germany). Dewaxed and rehydrated hippocampal sections were blocked in TBST (TBS with Tween20 ...
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bioRxiv - Cell Biology 2019Quote: ... embedded in 6% agarose and sectioned into 100 µm thin slices using a vibratome (VT1000 S, Leica). Tissue sections were further immunostained as described above.
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bioRxiv - Cell Biology 2020Quote: ... the wafers were coated with 6 nm of carbon via a high vacuum film deposition instrument (Leica) to avoid charging ...
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bioRxiv - Bioengineering 2022Quote: ... embedded in paraffin wax and sliced at a 6 μm thickness using a microtome (RM-2125RT, Leica). Slices were mounted on slides and stained with Picrosirius Red (PSR) ...
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bioRxiv - Neuroscience 2022Quote: ... embedded in 6% agarose cubes and cut into 60 µm coronal sections using a vibratome (Leica VT1200). Sections of Vglut2-ires2-FlpO/Chx10-Cre animals were immunhistochemically stained (primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: Breast tumor tissue was sectioned at 6 μm in a Leica CM 1850 Cryostat (Leica Microsystems GmbH). The sections were mounted on uncharged glass slides without the use of embedding media and placed immediately in 70% ethanol for 30 seconds ...
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bioRxiv - Neuroscience 2023Quote: ... Thin sections (70-90nm) were cut on a Leica Ultracut UC-6 ultramicrotome (Leica Microsystems, Wetzlar, Germany). Sections were collected on 3mm copper grids and subsequently stained for 30min with 2% aqueous uranyl acetate and for 5min with Reynold’s lead citrate ...
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bioRxiv - Physiology 2023Quote: ... Slides were sectioned at 6 µM on a Leica CM3050 S cryostat (Leica Biosystems; Deer Park, IL) and stored at −80°C until use ...
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bioRxiv - Genomics 2023Quote: ... CBA×C57BL/6 brains were sectioned on a cryostat (Leica, settings OT=-22°C, CT=-22°C) at 30µm thickness ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections (roughly 70 nm thick) were prepared using a Leica EM UC 6 ultramicrotome (Leica, Wetzlar, Germany) and observed using a JEM-1400 Flash transmission electron microscope (JEOL ...
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bioRxiv - Immunology 2024Quote: ... RV tissue was embedded in paraffin and 6 μm sections were cut using a microtome (Leica, Germany) and slides dried in a heated chamber ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit anti-Iba1 (Wako) ...
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bioRxiv - Plant Biology 2021Quote: ... the ears were embedded in 6% Agarose in PBS and sectioned at 100 μm using a vibratome (Leica) and fixed for 2 hours more at 4°C with gentle agitation ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). Embryonic and postnatal ventral midbrain tissues were fixed with 4% paraformaldehyde and dehydrated with 30% sucrose overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit mouse anti-sarcomeric α-actinin (SAA ...
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bioRxiv - Plant Biology 2019Quote: ... Ultrathin sections (∼90-nm) were taken longitudinally along the embedded root pieces using an ultramicrotome (UC-6; Leica) and mounted on nickel slot grids ...
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bioRxiv - Pathology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies are listed in Supplementary Table S2.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Thin sections of 6-8 μm were obtained from the paraffin block using a rotary microtome from Leica, Germany ...
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bioRxiv - Physiology 2023Quote: ... The embedded tissues were then sectioned into 6-μm slices at −20 °C using a CM1950 Cryostat (Leica), followed by fixing in 4% paraformaldehyde for 10 min ...
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bioRxiv - Physiology 2023Quote: ... The embedded tissues were then sectioned into 6- μm slices at −20 °C using a CM1950 Cryostat (Leica), followed by fixing in 4% paraformaldehyde for 10 min ...
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bioRxiv - Genomics 2024Quote: ... The resin blocks were sectioned at 6 μm thick by using a Leica SP1600 microtome (Leica Microsystems, Germany). The fluorescence signals of calcein (green ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were finally counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8).
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bioRxiv - Cell Biology 2019Quote: Quantification of protein by Western blotting was carried out using standard techniques and the following primary and secondary antibodies: anti-α-SMA (SMA-R-7-CE, Leica Biosystems, dilution: 1:100), anti-COL1 (1310-01 ...
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bioRxiv - Neuroscience 2023Quote: ... DCX+/CLR- and DCX-/CLR+ cells) were counted using Image J (Fiji 1.46) on 7 physical disectors obtained with confocal microscopy (Leica TCS SP5, oil immersion 63× objective) in each animal ...
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bioRxiv - Plant Biology 2020Quote: ... The division and elongation of AR founder cells were observed with a stereoscopic microscope (Leica MZ 6; Wetzlar, Germany) and ImageJ (v ...
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bioRxiv - Cell Biology 2020Quote: ... Resin was polymerised at 60°C overnight and 60 nm ultrathin sections were cut on an Ultracut 6 (Leica) ultramicrotome and imaged as described above.
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bioRxiv - Neuroscience 2019Quote: ... brains from 6-month-old 5xFAD and WT animals were coronally sectioned through the hippocampus on a vibratome (Leica; settings ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was embedded in 6% agarose and coronal sections (40 μm) were cut using a vibratome (Leica VT1200). A total of 30 sections starting from Bregma −1.22 mm (Paxinos and Franklin(2004) ...
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bioRxiv - Developmental Biology 2021Quote: Live imaging of Rx3::H2B-GFP-derived organoids (Video 5 and 6) was performed with Sp8 confocal microscope (Leica). Organoids were imaged from day 1 to day 2 at room temperature directly in low binding 96-well plate (Nunclon Sphera U-Shaped Bottom Microplate ...
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bioRxiv - Neuroscience 2022Quote: ... brains were embedded in 6% agarose and sliced coronally at 50 μm using a vibratome (Leica VT1000s; Wetzlar, Germany). Slices were stored in PBS and 0.05% sodium azide at 4 °C before staining.
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bioRxiv - Plant Biology 2022Quote: ... images were taken using 6-day old young seedling using Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems).
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bioRxiv - Biochemistry 2022Quote: ... Images were acquired as z sections at 0.27 μm (using Slidebook Software 6 from Intelligent Imaging Innovations or using LCS 3D software from Leica). Images were converted into maximal intensity projections ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 100µm were performed at medium speed (around 5) and amplitude (approx. 6) using a vibratome Leica VT1000S (Leica). Sections were placed on a glass slide for immunostaining.
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bioRxiv - Developmental Biology 2019Quote: ... Serial sections of 4–6 µm thickness were cut using a Leica RM2255 rotary microtome (Leica Microsystems GmbH, Germany) with disposable blades ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were sectioned at 50µm (1 in 6 series) in the coronal plane with a vibratome (model VT1000, Leica). Sections were stored at -20°C in a cryoprotectant solution comprised of 60% glycerol and 0.01% sodium azide in 0.1M PBS.
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bioRxiv - Cell Biology 2022Quote: ... fluorescence images were captured every 2 s for 6 min using fluorescence microscopy (#AF6000-DMI6B; Leica Microsystems, Wetzlar, Germany) (objective lens ...
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bioRxiv - Plant Biology 2020Quote: Plasmolysis was induced by mounting 6-day-old seedlings in 0.8 M mannitol on microscope slides and directly observed using confocal microscopy (Leica SP8). The proportion of the cell wall length in direct contact with the plasma membrane marker SYP122-mCitrine after plasmolysis was measured using Fiji after plasmolysis ...
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bioRxiv - Neuroscience 2020Quote: 200 μm-thick hippocampal sections were isolated from 16 C57BL/6 mice of P22-28 using a vibratome (Leica, VT1200S), in the presence of ice-cold oxygenated a-CSF (125mM NaCl ...
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bioRxiv - Immunology 2022Quote: Tissue samples were fixed for a minimum of 7 days in 10% neutralized buffered formalin before being removed from BSL-4 and subsequently processed in a Tissue-Tek VIP-6 automated vacuum infiltration processor (Sakura Finetek) and embedded in paraffin using a HistoCore Arcadia paraffin embedding machine (Leica). 5 μm tissue sections were generated using an RM2255 rotary microtome (Leica ...
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bioRxiv - Microbiology 2021Quote: ... The coverslips were fitted onto sample stubs using carbon adhesive tabs and sputter coated with 6 nm Iridium (Leica SCD500). Samples were imaged at 2 KV ...
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bioRxiv - Plant Biology 2021Quote: ... 6’-diamidino-2-phenylindole (DAPI) fluorescence signals were detected by confocal laser-scanning microscopy analysis using TCS SP8 (Leica Microsystems). All the images were obtained and processed with Leica LAS AF Lite software ...
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bioRxiv - Microbiology 2021Quote: Tissue samples were fixed for a minimum of 24 h in 4% PFA before being removed from BSL-3 and subsequently processed in a Tissue-Tek VIP-6 automated vacuum infiltration processor (Sakura Finetek) and embedded in paraffin using a HistoCore Arcadia paraffin embedding machine (Leica). 5 μm tissue sections were generated using an RM2255 rotary microtome (Leica ...
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bioRxiv - Immunology 2020Quote: ... Paraffin blocks were sectioned from posterior to anterior at 6-μm thickness using a rotary microtome (Leica RM2255, Wetzlar, Germany). Sections were stained using Safranin-O/Fast Green at 90-μm intervals to assess cartilage morphology in the medial tibial plateau ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were cut to either 10 μm slices for immunofluorescence staining or 4-6 μm for STORM (CM1950, Leica) to produce coronal brain sections.
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bioRxiv - Cell Biology 2023Quote: ... Transplanted embryos were mounted in 0.2% agarose in embryo medium and imaged between shield stage and 85% epiboly (6 - 9 hpf) under an inverted TCS SP8 confocal microscope (Leica) equipped with environmental chamber (Life Imaging Services ...
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bioRxiv - Cell Biology 2023Quote: ... and an Andor DU-888 X-11056 camera (1,024 x 1,024 pixels). Other live-cell images (Fig. 6) were acquired on confocal DIVE-FALCON laser scanning confocal microscope (Leica Microsystems) controlled by the Leica LAS X Life Science software using the HCX PL APO CS 63.0×/1.30 NA glycerol immersion objective and 488 nm and 633 nm lasers ...
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bioRxiv - Cell Biology 2024Quote: ... The embedded tissues were flash-frozen in liquid nitrogen for 1 minute and then sectioned into 6 μm-thick slices using a frozen microtome (Leica). The sections were stained with hematoxylin and eosin (Servicebio ...