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Citations for Leica :
351 - 400 of 444 citations for 7 Fluoro 1H indazole 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... and 3-8 randomly chosen fields per view per Matrigel assay were acquired using confocal Z-stack imaging (TCS-SP8; Leica). Attached pericytes were counted by using the LASX Software.
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bioRxiv - Cell Biology 2023Quote: ... Tissues from at least 3 independent mice were imaged using an SP5X laser scanning confocal microscope (Leica Microsystems CMS GmbH) or an LSM980 microscope and ZEN Desk 3.4 software (Carl Zeiss GmbH) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC ...
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bioRxiv - Developmental Biology 2023Quote: ... was injected into the cytoplasm of wild-type embryos at one-cell stage using an MPPI-3 microinjector (ASI) under a stereomicroscope (S9E, Leica). Injected embryos were incubated in the embryo medium (10% Hank’s with full strength calcium and magnesium ...
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bioRxiv - Neuroscience 2023Quote: ... until the brains sank in the solution (1-3 days) and at which point 35um thick hypothalamic sections were obtained using a cryostat (Leica). Sections were first incubated for 1 hour in blocking buffer (1X PBS with 2% BSA and 0.1% tween-20) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue blocks of the substantia nigra were then embedded in 3% agarose and sectioned using a vibratome (Leica, VT1000S, Germany).
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bioRxiv - Bioengineering 2023Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Neuroscience 2023Quote: ... Brains were post-fixed 24h in 4% PFA before being cut coronally and serially (10 series of 40 µm sections or 3 series of 100 µm sections for morphological studies) with a vibratome (Leica).
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bioRxiv - Cell Biology 2023Quote: ... Zeiss) equipped with a 100× /1.4 NA objective lens and a super-resolution confocal microscope (Leica TCS SP8 STED × 3) equipped with a 100× /1.4 NA objective lens were used for cell observation ...
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bioRxiv - Neuroscience 2023Quote: ... which were subsequently blotted for 3 seconds and vitrified by plunging into liquid ethane using an EM-GP2 plunge-freezer (Leica). Grids were then stored in liquid nitrogen until data collection.
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bioRxiv - Bioengineering 2023Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Neuroscience 2023Quote: ... fixed brains were embedded in 3% agarose and sectioned at 50 μm thickness with a vibrating microtome (Leica VT1000S Microtome). Free floating sections were incubated in 1% hydrogen peroxide for 15 minutes then incubated with anti-ATP synthase subunit C (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were either embedded in 3% agarose for coronal sectioning at 150 µm thickness on a vibrating microtome (VT1000S; Leica) or cryoprotected samples and sectioned coronally at 20 µm on a Leica CM3050 cryostat ...
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bioRxiv - Plant Biology 2024Quote: ... the eYFP fluorescence of fusion proteins was assayed 2–3 days after infiltration using a confocal microscope (Leica TCS SP8). For the Split–LUC assay ...
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bioRxiv - Immunology 2023Quote: ... A total volume of 3 µl was used for plunge freezing into liquid ethane using an automatic plunge freezer EM GP2 (Leica). The ethane temperature was set to −183 °C and the chamber to 24 °C with 80% humidity ...
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bioRxiv - Developmental Biology 2024Quote: ... Live VL3/VL4 NMJs in abdominal hemi-segment 3 were imaged in ambient temperature using a Stellaris 5 laser-scanning confocal microscope (Leica) with a water-immersion objective (HC FLUOTAR L VISIR 25x/0.95 ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were embedded within a 3% agarose (BP160-100, Fisher Bioreagents) mixture and sectioned in a vibratome (VT 1200, Leica) into 300-µm thick sections ...
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bioRxiv - Bioengineering 2024Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Biophysics 2021Quote: ... These dauer samples were then transferred to carriers of 3 mm diameter and 0.1 mm depth and rapidly frozen in a high-pressure freezing machine (Leica, EM ICE). For automated freeze substitution ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were fixed 6 hours and 3 days after seeding and imaged in brightfield using a Leica DMI 4000 B microscope (Leica Microsystems).
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bioRxiv - Cell Biology 2019Quote: Images of ZEB1 transgenic ZEB1+/+ and ZEB1+/- cell cultures at day 1 (sub-confluent) and at day 3 post-seeding (confluent) were acquired using the Leica DMIL LED inverted microscope (Leica Microsystems) and the N PLAN L 20x/0.35 PH1 objective ...
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bioRxiv - Neuroscience 2021Quote: ... i.p.) at P120 and P123 (after 3 days of monocular deprivation) Images were visualized using a custom Leica microscope (Leica Microsystems). Red light illumination was provided by 8 individually addressable LEDs (WS2812 ...
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bioRxiv - Cell Biology 2021Quote: ... was assembled on a Narishige micromanipulator model MM-3 (Narishige group) and was placed on the magnetic board of a Leica MS5 dissection microscope (Leica Biosystems), using a Narishige GJ-8 magnetic stand (Narishige group) ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Plant Biology 2020Quote: ... and germlings was measured after 15 min of exposure to NCR044.1 (50 μL, 3 μM) using fluorescence confocal microscopy (Leica SP8-X) with an excitation and emission wavelength of 488 and 508-544 nm ...
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bioRxiv - Cell Biology 2021Quote: ... whereas samples for STEM were attached to a sticky Post-It-Note (as described in (31)) and coated with 3 nm platinum and 4 nm carbon using an ACE600 coating device (Leica Microsystems). The STEM samples were then washed with Buffered Oxide Etchant (diluted 6:1 with surfactant ...
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bioRxiv - Cell Biology 2021Quote: ... around 1.5 µl of concentrated purified schizont pellet was transferred into aluminium or gold carriers (3 mm diameter, 100 or 200 µm depth; Leica Microsystems) and high pressure frozen with EM ice (Leica Microsystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... The glycolytic and oxidative portions of the TA muscles of 5 mice in the CTR group and 5 mice in the Crush - 3 days group were microdissected with a laser microdissection system (Leica LMD6) and recovered in RNAlater reagent (QIAGEN).
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in agarose (3% in Water) and 70 µm coronal sections were cut with a VT1000S vibratome (Leica Biosystems). Every second section ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were anesthetized with a mixture of oxygen (1L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Cell Biology 2019Quote: ... for 2 days at 4 °C and then transferred to 70% alcohol for 3-5 days before processed by using 4-hour tissue processing setting with Leica ASP6025 (Leica Biosystems). Placentas were embedded in paraffin (HistoCore Arcadia H and Arcadia C ...
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bioRxiv - Physiology 2020Quote: ... Finally, sections were incubated with DAB (3,3’-diaminobenzidine, Vector) for 3 min and counterstained with Gill’s hematoxylin (Leica, Buffalo Grove, IL) for 30 sec ...
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bioRxiv - Plant Biology 2020Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Microbiology 2021Quote: ... containing defined regions of the bacterial colonies were cut out and inverted on lecithin-coated 3-mm specimen carriers for high pressure freezing in a high- pressure freezing machine (Leica, ICE) with or without 10% polyvinylpyrrolidone as a filler (Δeps biofilms and one of the WT samples were frozen without filler) ...
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bioRxiv - Cell Biology 2019Quote: ... at least 3 adult worms for each condition were fixed using high pressure freezing (Leica EM PACT2, Leica Microsystems, Vienna, Austria) in 20% BSA (Sigma-Aldrich Co. ...
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bioRxiv - Plant Biology 2021Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Cell Biology 2021Quote: ... microscopy was performed with the 100×/1.4 Oil HC PL APO CS2 STED White objective of a Leica TCS SP8 3× STED microscope (Leica, Wetzlar, Germany) equipped with a white light laser ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed for 1–3 days and sectioned coronally (50–100 μm in thickness) using a vibratome (Leica; VT1000S). Brain sections were mounted on superfrost slides and coverslipped with ProLong antifade reagent with DAPI (Molecular Probes) ...
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bioRxiv - Plant Biology 2022Quote: ... were infiltrated in Nicotiana benthamiana leaves for transient expression and viewed after 3 days under confocal microscope Leica TCS SP5 (Leica Microsystems) equipped with appropriate lasers (514 nm -527 nm and 532 nm-588 nm for YFP and RFP ...
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bioRxiv - Cell Biology 2023Quote: ... The paraffin embedded clots were cut in 3 µm thick sections with a semi-automated rotary microtome (Leica Biosystems, Wetzlar, Germany) and placed on silanized glass slides ...
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bioRxiv - Neuroscience 2023Quote: ... cluster of differentiation 3 (CD3) and CD68 immunostaining was performed using a fully automated staining system (BOND-III; Leica Biosystems, UK). In brief ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections of 3 µm thickness were cut at the level of the hippocampus using a microtome (Leica Microsystems, Wetzlar, Germany). For immunohistochemistry either paraffin sections that were washed with 0.5% sodium azide (2 × 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 1h incubation in the same buffer containing the primary probes and a 3 min heat incubation step in a ThermoBrite slide hybridization system (Leica Biosystems), either at 75°C or 86°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Image acquisition was performed as z-stack images of 250 μm in depth in 3 separate channels using a Leica SP8 confocal microscope (Leica, Germany).
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bioRxiv - Biophysics 2023Quote: ... and then glued onto Leit-tabs (Plano, Germany) mounted on aluminum stubs (Plano, Germany) and sputter-coated with a 3 nm thin gold layer (ACD600, Leica, Austria). SEM images were acquired with a Zeiss Auriga FEG-SEM (Zeiss ...
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bioRxiv - Plant Biology 2023Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Plant Biology 2024Quote: ... and Antirrhinum majus stained with SR2200 and TO-PRO-3 iodide or DAPI was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Cell Biology 2024Quote: ... The tissues in paraffin blocks were subjected to sectioning (3-4 μM) by rotary type of microtome (Leica RM2255 Rotary Microtome, Leica Biosystems). The tissue sections were fixed in a glass slide and stained with hematoxylin and eosin (H&E ...
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bioRxiv - Biophysics 2019Quote: ... Then a bulk of muscle was washed 2 to 3 times in cold PBS and fixed on the specimen holder of VT1200S vibrating blade microtome (Leica Biosystems, US). The buffer tray of microtome was ice bathed and filled with cold PBS while slicing ...
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bioRxiv - Cancer Biology 2019Quote: ... homogeneous and mixed spheroids were generated and imaged repeatedly over time (2-3 times per week) using brightfield microscopy (4x objective; 0.3 NA, Leica DM IRBE, Hamamatsu). Spheroid volumes were calculated using SpheroidSizer58.