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Citations for Leica :
551 - 600 of 888 citations for 7 Chloro quinazoline 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed with 4% PFA in PB overnight and 50-µm coronal sections of the brainstem were obtained on a vibratome (Leica VT1200S). Expression of EGFP at the calyx of Held terminals was visualized using an epifluorescence inverted microscope (CKX41 ...
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bioRxiv - Microbiology 2023Quote: ... samples were stored light-protected in the same buffer at 4°C until imaging with a Leica TCS SP5 II laser scanning confocal microscope (Leica Microsystems) using sequential scanning mode.
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bioRxiv - Pathology 2023Quote: ... the specimens were paraffin-embedded and subsequently sectioned into 4-μm-thick tissue slices by a microtome (RM2235, Leica Microsystems Inc.). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... containing the dorsal hippocampus were cut in ice-cold (4 °C) choline chloride ACSF using a Leica VT1000 vibratome (Leica Biosystems) and transferred to warm (33 °C ...
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bioRxiv - Neuroscience 2023Quote: ... sagittal sections at 5° angle tilted to the midline with 300 μm thickness were obtained inside the same slicing solution at 4°C using Leica VT1200S vibrotome (Leica Biosystems). Slices were immediately transferred to a chamber filled with artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2023Quote: ... paraffin blocks were then sliced in coronal orientation at a thickness of 4-5 µm on a semiautomated rotary microtome (Leica, Germany). These sections were then collected ...
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bioRxiv - Cancer Biology 2023Quote: ... Paraffin blocks were then sectioned at 4 μm thickness and processed for H&E staining and the 20X objective of the Aperio-XT slide scanner (Leica Biosystems) was used to scan slides ...
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bioRxiv - Neuroscience 2023Quote: ... The L4 segments were fixed overnight at 4°C and cut into 60 μm thick transverse sections with a vibrating blade microtome (Leica VT1200). The sections were incubated for 3 days in rabbit anti-GFP (see Table S2) ...
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bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
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bioRxiv - Immunology 2023Quote: ... from Apoe-/-LysMCre and Apoe-/- LysMCreCnr1flox/flox male mice collected after 4 weeks WD were mounted on RNase-free membrane metal frame slides (POL FrameSlide, Leica Microsystems). Following deparaffinization under RNase-free conditions ...
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bioRxiv - Neuroscience 2023Quote: ... After the brain samples were fixed in 4% paraformaldehyde overnight, they were dehydrated in 30% sucrose (Daejung, Korea) and embedded in frozen section compound (Leica, Germany). Coronal sections (10 µm ...
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bioRxiv - Cell Biology 2023Quote: ... confocal microscope in 3 Z-stacks at 20x (1024x1024 resolution) and further imaged at 63x with immersion oil in 4 Z-stacks to highlight regions of interest (Leica Sp8x).
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bioRxiv - Neuroscience 2023Quote: ... Half brains were fixed in 4% paraformaldehyde for 24h and sectioned at 40 μm thickness in the coronal plane on a vibratome (Leica VT1000S).
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bioRxiv - Neuroscience 2023Quote: ... Brains were removed and postfixed overnight at 4°C and then cut into 50 µm coronal sections using a vibratome (#TPI1000; Ted Pella, or VT1000P; Leica). Sections were collected in cryoprotectant (25% glycerol ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating coronal sections with a vibrating microtome (Leica, VT1000). All the following steps were done with gentle shaking ...
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bioRxiv - Microbiology 2024Quote: ... Grids were sputter-coated with a ∼4 nm thick layer of tungsten using an ACE600 cryo-sputter coater (Leica Microsystems GmbH) and afterwards transferred into a Crossbeam 550 FIB-SEM dual-beam instrument (Carl Zeiss Microscopy) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The other hemibrain was drop-fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) and sectioned (30 μm) with a sliding microtome (Leica SM2000R). All mouse experiments were performed in accordance with European Union Council Directive 86/609/EEC ...
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bioRxiv - Cell Biology 2024Quote: ... The tissues in paraffin blocks were subjected to sectioning (3-4 μM) by rotary type of microtome (Leica RM2255 Rotary Microtome, Leica Biosystems). The tissue sections were fixed in a glass slide and stained with hematoxylin and eosin (H&E ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5-µm thick sections using a microtome (RM2155, Leica, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin (HE ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were stored in 4% formaldehyde for 24h at 4°C after which they were transferred to 30% sucrose solution at 4°C until they were cut in 50 μm thick coronal slices with a cryostat (Leica, Germany). The brain slices were washed twice for 10 minutes with phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... The frozen samples were subsequently transferred into a Leica EM AFS-2 freeze substitution unit (Leica Microsystems). Over a period of five days ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.05% uranyl acetate in acetone and placed into an AFS-2 Freeze Substitution Machine (Leica Microsystems, Austria). Samples were freeze-substituted at −90°C for 72 h ...
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bioRxiv - Physiology 2021Quote: ... CFP and DsRed fluorescence signal were detected after 2 days with a CLSM (Leica AF6000 modular microsystems). All experiments were biologically repeated for three times.
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bioRxiv - Plant Biology 2022Quote: ... seedlings were mounted on a glass slide in 1/2 MS medium and imaged on SP8 (Leica). YFP was excited by the 514-nm line of the argon laser and detected between 525 and 580 nm ...
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bioRxiv - Neuroscience 2021Quote: ... or inferior colliculus were cut at 300 µm with a vibratome (VT1200S, Leica, or 7000smz-2, Campden) in warm aCSF ...
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bioRxiv - Plant Biology 2021Quote: ... the embedded samples were sectioned at 2–5 μm thickness using a rotary microtome (Leica, Wetzlar, Germany) and stained with basic fuchsin ...
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bioRxiv - Neuroscience 2020Quote: ... Antigen retrieval was done by incubation with Bond Epitope Retrieval solution 2 (AR9640, Leica Biosystems, Wetzler, Germany) for 20 minutes and then sections were then washed with Bond Wash solution 3x ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 % glutaraldehyde and 0.25 % uranyl acetate for 60 h in a FS system (AFS-2, Leica Microsystems). Larvae were embedded in an Epon-Araldite mix (hard formula ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides for CD14 and CD163 stains were retrieved for 20 minutes using Epitope Retrieval 2 (EDTA; Leica) incubated in protein block (Rodent Block M ...
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bioRxiv - Bioengineering 2022Quote: ... and counted for expression of opie-2-dsRed and 3xP3-tdTomato using a fluorescent stereoscope (Leica M165FC). The difference in total larval counts compared to total egg counts were considered to be dead during embryonic or early larval stages ...
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bioRxiv - Cell Biology 2022Quote: ... 0.05% uranyl acetate in acetone and placed in an AFS-2 Freeze-Substitution Machine (Leica Microsystems, Vienna). Samples were freeze-substituted at -90°C for 72 h ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in an FS system (AFS-2; Leica Microsystems). The embryos were embedded in an Epon-Araldite mix (EMS hard formula) ...
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bioRxiv - Immunology 2022Quote: ... Paraffin embedded kidney sections (2 µm) were cut on a Leica RM 2165 microtome (Leica, Wetzlar, Germany) and dried at 60°C for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in a FS system (AFS-2, Leica Microsystems). Larvae were embedded in Epon-Araldite mix (EMS hard formula) ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... 2 μm thin slides were then prepared and immunohistochemistry was performed on a Bond Rxm system (Leica) with primary antibodies against Hexokinase 1 (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... Semi thin (2 μm) and ultra-thin (70 nm) sections were cut with an ultra-microtome (Leica Ultracut ...
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bioRxiv - Cell Biology 2023Quote: ... images were captured every 2 h for 72 h by the IncuCyte Live-Cell analysis system (Leica).
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bioRxiv - Cancer Biology 2023Quote: ... Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitope Retrieval Solution 2, Leica Biosystems, cat# AR9640).
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bioRxiv - Cell Biology 2019Quote: ... submersed in the media chamber containing 4°C Phosphate buffered Saline and cut using a Leica VT1200S vibrating blade microtome (Leica Biosystems, UK) at a speed 0.3mm/sec ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in 4% low temperature gelling agarose and cut into 100-150 micrometer thick section using a vibratome (Leica VT1200 S). The Proximity Ligation Assay (PLA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Cancer Biology 2021Quote: Larvae fixed in 4% PFA overnight at 4°C were embedded in 4% low melting temperature agarose and transverse sections collected at 200 µm intervals using a vibrating microtome (Leica VT 1000S). Sections were blocked with 1% BSA in PBS/0.3% Triton X-100 and incubated with a 1:750 dilution of mAb414 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections from V1 were mounted on gelatin-coated slides and cover-slipped with Prolong Diamond Antifade Mounting Media (P36965 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed for 24 hours and then included in agar 4% and serial cuts of 40 µm thick were made using vibratome (Leica Biosystems, S2000).
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bioRxiv - Cancer Biology 2021Quote: ... The specimens were embedded in paraffin blocks and serial sections of 4 μm thickness were cut using a microtome (Leica Biosystems, Germany). The sections were stained by Hematoxylin & Eosin (H&E)34 Histological photographs were obtained using a digital light microscope (Olympus ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were postfixed in 4 % PFA for 24 hours until they were sliced (70 µm thickness) using a Leica Vibratome (Leica, Wetzlar, Germany). Slices were directly mounted on an object slide (Gerhard Menzel ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were post-fixed overnight in 4% paraformaldehyde and then cryoprotected in a 30% sucrose and 0.1M PBS solution prior to sectioning (Leica CM3050S, Leica Biosystems). Three sets of immediately adjacent sections (40 μm ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections with LGN and V1 were then mounted on gelatin-coated slides and cover-slipped with mounting media (Prolong Diamond Antifade Mounting Media ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...